Multiple-bead assay for the differential serodiagnosis of neglected human cestodiases: Neurocysticercosis and cystic echinococcosis.

BACKGROUND: Neurocysticercosis (NCC), and cystic echinococcosis (CE) are two neglected diseases caused by cestodes, co-endemic in many areas of the world. Imaging studies and serological tests are used in the diagnosis of both parasitic diseases, but cross-reactions may confound the results of the latter. The novel multiplex bead-based assay with recombinant antigens has been reported to increases the diagnostic accuracy of serological techniques. METHODOLOGY: We set-up an immunoassay based on the multiplex bead-based platform (MBA), using the rT24H (against Cysticercus cellulosae, causing cysticercosis) and r2B2t (against Echinococcus granulosus sensu lato, causing CE) recombinant antigens, for simultaneous and differential diagnosis of these infections. The antigens were tested on 356 sera from 151 patients with CE, 126 patients with NCC, and 79 individuals negative for both diseases. Specificity was calculated including sera from healthy donors, other neurological diseases and the respective NCC or CE sera counterpart. The diagnostic accuracy of this assay was compared with two commercial ELISA tests, Novalisa and Ridascreen, widely used in the routine diagnosis of cysticercosis and CE, respectively. MAIN FINDINGS: For the diagnosis of NCC, sensitivity ranged from 57.94-63.49% for the rT24H-MBA, and 40.48-46.03% for Novalisa ELISA depending on exclusion or inclusion of sera having equivocal results on ELISA from the analysis; specificities ranged from 90.87-91.30% and 70.43-76.96%, respectively. AUC values of the ROC curve were 0.783 (rT24H) and 0.619 (Novalisa) (p-value < 0.001). For the diagnosis of CE, the sensitivity of the r2B2t-MBA ranged from 68.87-69.77% and of Ridascreen ELISA from 50.00-57.62%; specificities from 92.47-92.68% and from 74.15-80.98%, respectively. AUC values were 0.717 and 0.760, respectively. CONCLUSIONS/SIGNIFICANCE: Overall, the recombinant antigens tested with the bead-based technology showed better diagnostic accuracy than the commercial assays, particularly for the diagnosis of NCC. The possibility of testing the same serum sample simultaneously for the presence of antibodies against both antigens is an added value particularly in seroprevalence studies for cysticercosis linked to control programs in endemic areas where these two parasites coexist.

Los contenidos de la formación parental y sus implicaciones en el comportamiento de los adolescentes: elementos desde una revisión / Contents of parental training and its implications for adolescent behavior: elements from a review

Resumen Los padres buscan apoyo para enfrentar los desafíos que sus hijos tienen en la adolescencia. El objetivo de este trabajo es analizar los tópicos abordados en la formación de padres con hijos adolescentes y su relación con las tendencias comportamentales de estos. Para ello, se llevó a cabo una revisión cualitativa de intervenciones y programas centrados en fortalecer el proceso de crianza, estableciéndose una concentración en los aspectos regulatorios, comunicativos y emocionales de la relación padre-hijo. Por medio de un análisis cuantitativo, también se pudo establecer que los tópicos abordados tienen efectos significativos sobre los adolescentes, como la reducción en problemas de comportamiento, manejo adecuado de la sexualidad y disminución en la tendencia al consumo de sustancias. Teniendo en cuenta la información registrada, es posible plantear que la formación parental puede abordarse en diferentes formatos de acuerdo con los objetivos y condiciones disponibles, lo que puede representar beneficios importantes para el desarrollo saludable de los adolescentes.

Abstract Parents seek support to cope with the challenges their adolescents face. The objective of this paper is to analyze the topics addressed in the training of parents and their relation with adolescent behavioral tendencies. A qualitative review of interventions and programs focused on strengthening the parenting process was carried out, establishing a concentration on the regulatory, communicative and emotional aspects of the parent-child relationship. Through a quantitative analysis, it was also identified that the topics addressed have significant effects on adolescents, such as the reduction of behavioral problems, adequate management of sexuality and a decrease in the tendency to use substances. With this considered, it is possible to state that parental training can be addressed in different formats according to the objectives and conditions available, which can represent important benefits for the healthy development of adolescents.

Patrones de la oomposición de aves acuáticas enel río la vieja, valle geográfiico del río cauca, Colombia / Composition Patterns of Waterbirds from La Vieja River, Geographic Valley of Cauca River, Colombia

Compilamos y analizamos los datos de observaciones realizadas entre los años 2001 y 2013 en tres sectores a lo largo del cauce del río La Vieja, ubicado en el valle geográfico del río Cauca, Colombia. Describimos los datos espacial y temporalmente, enfocándonos en identificar patrones de composición y abundancia de especies. Registramos 28 especies de aves acuáticas en 33 transectos, siendo 22 especies observadas en más del 50 % de los transectos. Las diferencias en el número de especies por transecto no fueron significativas entre los tres sectores del río. Sin embargo, dos análisis de agrupamiento, considerando tanto los datos de presencia/ausencia como de abundancia, indicaron que la composición de especies presenta estructura espacial a lo largo del río. En contraste, aunque las observaciones se realizaron durante más de diez años no se encontró evidencia de cambios temporales en la composición de especies. No obstante, algunas especies mostraron tendencias de aumento o disminución en su frecuencia. Presentamos el nuevo registro de una especie (Chloroceryle aenea) para la región. A pesar de que el paisaje asociado al río La Vieja ha sufrido un gran impacto antropogénico, aún conserva una diversidad significativa de aves acuáticas que pueden servir para aportar valor a los planes de conservación en la zona.

We compiled and analyzed data gathered from observations during the period 2001-2013 in three sectors along La Vieja river, located in the Cauca river valley, Colombia. We describe spatial and temporal aspects of such dataset, focusing in indentify patterns of species' composition and abundance. We recorded 28 waterbird species in 33 transects, being 22 species observed in more than 50 % of these transects. The species richness among transects did not shows significant differences. However, two cluster analyses, considering both presence/absence and abundance data, showed that there is spatial structure in the species composition along the river. In contrast, although observations were conducted during more than ten years there is no evidence of temporal changes in species composition. Still, some species showed increase or decrease trends in their frequency. We present a new record for one species (Chloroceryle aenea) for the region. Despite that the landscape surrounding La Vieja river have faced a high anthropogenic impact, the river still presents a significant diversity of waterbirds, which could add value to the conservation plans in the zone.

Inmunolocalización hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia Oreochromis spp) / Liver immunolocation of CYP P450 in an subacute toxicity experiment with Clorpirifos o,o-(3, 5, 6- trichloride -2-pyridyl phosphorotioato in young male tilapia (Oreochromis spp) / Inmunolocalização hepática de CYP P450 num teste de toxicidade subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) em machos juvenis de tilápia (Oreochromis spp.)

Resumen En Colombia son escasas las técnicas de diagnóstico implementadas con biomarcadores inmunohistoquímicos para realizar biomonitoreo de contaminación ambiental. Objetivo: Implementar una técnica inmunohistoquímica para inmunolocalizar CYP 1A en tejidos de peces, luego de realizar un experimento de exposición subaguda al Clorpirifos en tilapias juveniles machos. Métodos: el experimento de dosis subletal, se realizó en un sistema semiestático, con recambio diario del 50% del volumen de agua manteniendo la concentración correspondiente en cada grupo experimental mediante la adición de la mitad de la dosis hasta el día 28. Las concentraciones de Clorpirifos para la exposición fueron 4, 8, y 12 μg/L, en los grupos tratados y 0,0 μg/L en el grupo control. Se tomaron muestras para estudio histopatológico e inmunohistoquímico. Resultados: se encontró diferencia significativa (p<0,05) para algunas lesiones en el hígado, confirmando que este es un órgano de impacto de los efectos del Clorpirifos a bajas dosis. Se verificó la inducción de CYP 1A en el hepatocitos, endotelio y células biliares tanto de los animales expuestos como en los no expuestos. Desde el punto de vista multidimensional no se encontró diferencia estadística entre los tratamientos para las variables estudiadas. Conclusión: se implementó y normalizó una técnica de inmunohistoquímica para la inmunolocalización tisular de CYP 1A, que podrá utilizarse en futuras investigaciones para realizar biomonitoreo de contaminación en las cuencas hidrográficas de Colombia.

Abstract In Colombia the diagnostic techniques for implementing immunohistochemical biomarkers to fulfill biomonitoring of environmental contamination are limited. Objective: To implement an immunohistochemical technique to immunolocate CYP 1A in the tissue of fish, performing a subacute exposition experiment of Clofopirifos in the liver of young male tilapia. Methods: the sublethal dosage was carried out in semi-static state, with a daily refill of 50% of the water volume, maintaining the corresponding concentration in each group through the addition of half of the dosage until the 28th day. The Cloropirifos concentrations for the exposition were 4, 8, and 12 μg/L in the treated groups and 0.0 μg/L in the control group. Samples were taken for histopathological and immunohistochemical study. Results: A significant difference (p<0.05) for some liver lesions, confirming that it is an organ affected by low dosages of Cloropirifos. It verified that the induction of CYP 1A in hepatocyte, endothelium and biliary cells equally in exposed animals and those that weren't exposed. From a multidimensional perspective, no statistical difference was found between the treatments for the variables under study. Conclusion: this study implemented and normalized an immunohistochemical technique for the tissue immunolocation of CYP 1A that could be used in future investigations to fulfill biomonitoring of contamination in Colombia's hydrographic basins.

Resumo Na Colômbia são escassas as técnicas de diagnóstico realizadas com biomarcadores imuno-histoquímicos para realizar biomonitoramento da contaminação ambiental. Objetivo: Estandardizar uma técnica imuno-histoquímica para fazer imunolocalização do CYP 1ª em tecidos de peixes, logo de realizar um experimento de exposição subaguda ao Clorpirifós em machos juvenis de tilápia. Métodos: O teste da dose subletal realizou-se num sistema semiestático, com recambio diário de 50% do volume de água mantendo a concentração correspondente em cada grupo experimental mediante a adição da metade da dose até o dia 28. As concentrações de Clorpirifós para a exposição dos peixes foram 4, 8 e 12 μg/L, nos grupos tratados e 0,0 μg/L no grupo controle. Tomaram-se amostras para o estudo histopatológico e imuno-histoquímico. Resultados: Encontrou-se diferença significativa (p<0.05) para lesões no fígado, confirmando que este é um órgão que recebe diretamente o impacto dos efeitos do Clorpirifos em baixas doses. Verificou-se a indução de CYP 1A nos hepatócitos, endotélio e células biliares tanto nos animais que estiveram expostos ao Clorpirifós quanto nos animais que não estiveram expostos. Desde o ponto de vista multidimensional não se encontrou diferença estadística entre os tratamentos para as variáveis estudadas. Conclusão: Estandardizou-se e normalizou-se uma técnica de imuno-histoquímica para a imunolocalização tissular de CYP 1A, que poderá se utilizada em futuras pesquisas para realizar biomonitoramento da contaminação das bacias hidrográficas da Colômbia, além disto.