RESUMO
The aim of this study was to determine, using multiple logistic models, factors affecting pregnancy per AI (P/AI) following fixed-time artificial insemination (FTAI) in repeat breeders cows (RBC) treated with rbST throughout lactation. 498 RBC received a CIDR device and 100µg of GnRH on day 0. CIDR removal and PGF(2α) (25mg) treatment were done concurrently on day 7. Estradiol benzoate (EB, 1mg) was injected on day 8 and GnRH on day 9; cows were inseminated 16-20h later. Cows with an average milk protein <3% were 43% more likely (P<0.05) to become pregnant at FTAI than cows with milk protein ≥3%. Cows with <6 services had significantly increased chances of becoming pregnant than cows with ≥6 services at FTAI (P/AI 36 vs. 27%; P<0.05). CIDR-treated cows with less than three lactations were 1.7 times more likely (P/AI 35 vs. 21%; P<0.05) to become pregnant than cows in third or greater lactation. Cows with peak milk yields lower than 55kg were 1.5 times more likely to get pregnant than cows with peak milk yields greater than 55kg (P/AI 37 vs. 28%; P<0.05). P/AI was lower (30 vs. 35%; P<0.01) for cows with dry periods <62 days than cows with dry periods ≥62 days. Cows subjected to FTAI with a temperature-humidity index (THI) <76 were 45% more likely (P<0.05) to become pregnant than cows inseminated at a THI≥76. It was concluded that an acceptable proportion (32%) of RBC can become pregnant with the protocol used in the present study. Also, subfertility in CIDR-treated cows was associated with high peak yields, high milk protein, increased service, increased lactation, high THI at AI and short dry periods.
Assuntos
Bovinos , Inseminação Artificial/veterinária , Indução da Ovulação/métodos , Resultado da Gravidez/veterinária , Prenhez , Administração Intravaginal , Animais , Cruzamento/métodos , Bovinos/fisiologia , Causalidade , Indústria de Laticínios , Sincronização do Estro/métodos , Feminino , Inseminação Artificial/fisiologia , Dispositivos Intrauterinos Medicados/veterinária , Indução da Ovulação/veterinária , Paridade/fisiologia , Gravidez , Resultado da Gravidez/epidemiologia , Progesterona/administração & dosagemRESUMO
The current study evaluated the effect of glutamate supply on the onset of puberty and possible links to changes in serum concentrations of insulin [INS], glucose [GLU] and triiodothyronine [T(3)]. The study was conducted from June to November in prepuberal female goats (n=18; 3 mo. old, 7/8 Saanen-Alpine, 1/8 Criollo, 26° north) randomly assigned to two experimental groups: (i) excitatory amino acids group (group AA, n=10; 16.52±1.04 kg LW, 3.4±0.12 body condition score [BCS], receiving an intravenous infusion of 7 mg kg(-1) live weight [LW] of l-glutamate, twice a week, and (ii) control group (group CC, n=8; 16.1±1.04 kg LW, 3.1±0.12 BCS) receiving saline. Blood samples were obtained twice a week, for assessing progesterone [P(4)], as well as in a monthly basis to evaluate INS and T(3) by RIA. Mean final LW and BCS were 23.2±0.72 kg, 3.53±0.10 units, without differences between groups. The AA group depicted an earlier onset of puberty (6.9±0.3 compared to 7.5±0.4 mo.; P<0.05) and an increased ovarian activity (70±0.28% compared to 25±0.26%; P<0.05). Neither serum INS concentrations nor serum glucose concentrations differed between treatments (1.2±0.06 ng mL(-1) and 89.6±1.8 mg 100 mL(-1); P>0.05, respectively). Serum T(3) concentrations, however, were greater in AA goats (1.55±0.03 compared to 1.39±0.04 ng mL(-1)). In addition, a treatment x time interaction occurred (P<0.05) across the experimental period for both T(3) and INS, with increases by the last third of the experimental period, time at which the onset of puberty occurred in both experimental groups. No differences (P>0.05) for glucose concentrations across time occurred between treatments. Results indicate that, in prepuberal goats, glutamate acts as a cue for sexual maturation in a glucose-independent pathway, while both T(3) and INS seem to act as metabolic modulators for the establishment of puberty in goats. Actions of INS and T(3) are mediated directly on hypothalamic centers regulating the pulsatile release of GnRH or indirectly by peripheral cues reflecting INS-T(3) actions on somatic development remains to be determined.