Modulating cultivation regimes of Messastrum gracile SVMIICT7 for biomass productivity integrated with resource recovery via hydrothermal liquefaction.

The present study was designed to assess Messastrum gracile SVMIICT7 potential in treating dairy wastewater (autoclaved (ADWW) and raw (DWW)) with relation to nutrient removal, in-vivo Chl-a-based biomass, and bio-oil synthesis. Chlorophyll a fluorescence kinetics revealed improved photochemical efficiency (0.639, Fv/Fm) in M. gracile when grown with DWW. This may be owing to enhanced electron transport being mediated by an effective water-splitting complex at photosystem (PSII) of thylakoids. The increase in ABS/RC observed in DWW can be attributed to the elevated chlorophyll content and reduced light dissipation, as evident by higher values of ETo/RC and a decrease in non-photochemical quenching (NPQ). M. gracile inoculated in DWW had the highest Chl-a-biomass yield (1.8 g L-1) and biomolecules while maximum nutrient removal efficiency was observed in ADWW (83.7% TN and 60.07% TP). M. gracile exhibited substantial bio-oil yield of 29.6% and high calorific value of 37.19 MJ kg-1, predominantly composed of hydrocarbons along with nitrogen and oxygen cyclic compounds. This research offers a thorough investigation into wastewater treatment, illustrating the conversion of algal biomass into valuable energy sources and chemical intermediates within the framework of a biorefinery.

Biorefinery-centric ethanol and oleochemical production employing Yarrowia lipolytica and Pichia farinosa.

The research examined the capabilities of Yarrowia lipolytica (YL) and Pichia farinosa (PF) in converting sugars to ethanol and oleochemicals. Lipid, ethanol, protein yield and gene-expressions were analysed at different substrate concentrations (3 to 30 g/L) with glucose, food waste, and fermentation-effluent. Optimal results were obtained at 20 g/L using both synthetic carbon with 4.6 % of total lipid yield. Lauric and Caprylic acid dominance was noted in total lipid fractions. Protein accumulation (6 g/L) was observed in glucose system (20 g/L) indicating yeast strains potential as single-cell proteins (SCP). Fatty-acid desaturase (FAD12) and alcohol dehydrogenase (ADH) expressions were higher at optimum condition of YL (1.15 × 10-1, 3.8 × 10-2) and PF (5.8 × 10-2, 3.8 × 10-2) respectively. Maximum carbon reduction of 87 % depicted at best condition, aligning with metabolic yield. These findings highlights promising role of yeast as biorefinery biocatalyst.

Catalytic hydrothermal deoxygenation of sugarcane bagasse for energy dense bio-oil and aqueous fraction acidogenesis for biohydrogen production.

The study focuses on the effective conversion of sugarcane bagasse (SCB) by catalytic deoxygenation using various alkali and metal-based catalysts under N2 pressure employing water as solvent. The specific influence of catalyst over bio-crude yields (bio-oil and aqueous fraction) including energy recovery ratio was explored. The optimum catalytic condition (Ru/C) resulted in âˆ¼ 70% of bio-crude and 28% of bio-oil with an improved HHV (31.6 MJ/kg) having 11.6% of aliphatic/aromatic hydrocarbons (C10-C20) which can be further upgraded to drop-in fuels. The biocrude composed of 44% of aqueous soluble organic fraction (HTL-AF). Further, the carbon-rich HTL-AF was valorized through acidogenic fermentation to yield biohydrogen (Bio-H2). The maximum bio-H2 production of 201 mL/g of TOC conversion (K2CO3 catalyst) was observed with 7.7 g/L of VFA. The SCB was valorized in a biorefinery design with the production of fuels and chemical intermediates in a circular chemistry approach.

Dual-stage biorefinery to convert spentwash hydrolysate into oleochemicals using Trichosporon cutaneum and Yarrowia lipolytica.

Yeast lipids from low-cost renewable feedstock are valuable resources for oleochemicals thus enabling circular chemistry. Current study focuses on lipid and volatile fatty acid (VFA) production through dual-stage fermentation of spentwash in a biorefinery framework with Trichosporon cutaneum (Tc) and Yarrowia lipolytica (Yl). During cell proliferation phase, Tc and Yl accumulated 2.9 and 2.5 g/L of dry biomass respectively in acid-hydrolysed spentwash (AHSW) and produced 16 and 5.5 g/L of total VFA respectively. Lipid yields (29.8%) and lipid titres (0.89 g/L) were higher in Tc/AHSW, when compared to Yl indicating the efficacy of Tc in spentwash bioremediation. Lipid accumulation was enhanced to 35% in Tc/AHSW, in presence of 0.05% NH4Cl due to oxidative stress of ammonium ions. Analysis of fatty acid composition revealed the presence of higher oleic acid, which is ideal for biodiesel production. The results demonstrate a sustainable biorefinery model for bioremediation of spentwash and its value addition.

Design and evaluation of gas fermentation systems for CO2 reduction to C2 and C4 fatty acids: Non-genetic metabolic regulation with pressure, pH and reaction time.

Addressing the carbon emissions through microbial mediated fermentation is an emerging interest. Custom designed and fabricated gas fermentation (GF) systems were evaluated to optimize the headspace pressure, pH (6.5, 7.5, and 8.5), fermentation time, and substrate concentration by employing enriched homoacetogenic chemolithoautotrophs in non-genetic approach. Headspace pressure showed marked influence on the metabolic conversion of inorganic carbon to acetic and butyric acids with 26% higher productivity than the control (atmospheric pressure). Maximum volatile fatty acid (VFA) yield of 3.7 g/L was observed at alkaline pH (8.5) under 2 bar pressure at carbon load of 10 g/L, 96 h). Acetic (3.0 g/L) and butyric (0.7 g/L) acids were the major products upon conversion of 85% of the inorganic substrate. A better in-situ buffering (ß = 0.048) at pH 8.5 along with higher reductive current (RCC: -4.4 mA) depicted better performance of GF towards CO2 reduction.

Green Hydrogen-Compressed natural gas (bio-H-CNG) production from food waste: Organic load influence on hydrogen and methane fusion.

Biogenic hydrogen (bioH2) enriched compressed natural gas (bio-H-CNG or biohythane) is emerging interest due to its feasibility to use in the existing transportation infrastructure with induced environmental benefits. This study evaluated the production of bioH2and biomethane (bioCH4) towards bio-H-CNG formation at a varying organic load (OL: 30,40,50 g COD/L) of food waste (FW). Acidogenic reactor operated with FW at 40 g COD/L showed the highest cumulative bioH2production while elevated OL (50 g COD/L)showedhigher cumulative bioCH4production (CMP: 11.92 L) from the methanogenic reactor. BioH2 and bioCH4 produced at different time intervals were combined to assess bio-H-CNG. The nature of biocatalyst and OLsignificantly regulated the composition of bio-H-CNG varying between 0.1 and 0.3 of H2/(H2+CH4) ratio accounting for5-12.6 kJ/g COD. Chain elongation, converting short (C2-C4) to medium-chain fatty acids(Caproic acid,1.16 g/L) was specifically observed during the acidogenic process.

Microalgal Cell Biofactory-Therapeutic, Nutraceutical and Functional Food Applications.

Microalgae are multifaceted photosynthetic microorganisms with emerging business potential. They are present ubiquitously in terrestrial and aquatic environments with rich species diversity and are capable of producing significant biomass. Traditionally, microalgal biomass is being used as food and feed in many countries around the globe. The production of microalgal-based bioactive compounds at an industrial scale through biotechnological interventions is gaining interest more recently. The present review provides a detailed overview of the key algal metabolites, which plays a crucial role in nutraceutical, functional foods, and animal/aquaculture feed industries. Bioactive compounds of microalgae known to exhibit antioxidant, antimicrobial, antitumor, and immunomodulatory effects were comprehensively reviewed. The potential microalgal species and biological extracts against human pathogens were also discussed. Further, current technologies involved in upstream and downstream bioprocessing including cultivation, harvesting, and cell disruption were documented. Establishing microalgae as an alternative supplement would complement the sustainable and environmental requirements in the framework of human health and well-being.

Tunable production of succinic acid at elevated pressures of CO2 in a high pressure gas fermentation reactor.

Production of bio-based alternative of succinic acid (SA) has been growing due to the awareness on environmental advantages it offers, such as CO2 sequestration. Current study focuses on evaluating the impact of different CO2 partial pressures (0.6, 0.8, 1, 2 bar) on SA production and yield as well as on other parameters like acids profile and CO2 fixation rate in Citrobacter amalonaticus. Increasing partial pressure to 2 bar enhanced SA production and maximum of 14.86 gL-1 was achieved with a productivity of 0.36 gL-1h-1 and yield of 52.10%. Varying partial pressures depicted significant influence on total acids profile, where at lower pressures (0.6 bar) lactic (5.6 gL-1) and acetic acids (4.1 gL-1) became dominant products, while concentration of SA was 2.07 gL-1, by the end of cycle. The desirable effect of moderately elevated pressures for converting CO2 to platform chemicals can be a potential strategy in overcoming current challenges related to CO2 abatement.

Recent advances in nutrient removal and recovery in biological and bioelectrochemical systems.

Nitrogen and phosphorous are key pollutants in wastewater to be removed and recovered for sustainable development. Traditionally, nitrogen removal is practiced through energy intensive biological nitrification and denitrification entailing a major cost in wastewater treatment. Recent innovations in nitrogen removal aim at reducing energy requirements and recovering ammonium nitrogen. Bioelectrochemical systems (BES) are promising for recovering ammonium nitrogen from nitrogen rich waste streams (urine, digester liquor, swine liquor, and landfill leachate) profitably. Phosphorus is removed from the wastewater in the form of polyphosphate granules by polyphosphate accumulating organisms. Alternatively, phosphorous is removed/recovered as Fe-P or struvite through chemical precipitation (iron or magnesium dosing). In this article, recent advances in nutrients removal from wastewater coupled to recovery are presented by applying a waste biorefinery concept. Potential capabilities of BES in recovering nitrogen and phosphorous are reviewed to spur future investigations towards development of nutrient recovery biotechnologies.

Retrofitting hetrotrophically cultivated algae biomass as pyrolytic feedstock for biogas, bio-char and bio-oil production encompassing biorefinery.

Algal biomass grown hetrotrophically in domestic wastewater was evaluated as pyrolytic feedstock for harnessing biogas, bio-oil and bio-char. Freshly harvested microalgae (MA) and lipid extracted microalgae (LEMA) were pyrolysed in packed bed reactor in the presence and absence of sand as additive. MA (without sand additive) depicted higher biogas (420 ml/g; 800 °C; 3 h) and bio-oil (0.70 ml/g; 500 °C; 3 h). Sand addition enhanced biogas production (210 ml/g; 600 °C; 2 h) in LEMA operation. The composition of bio-gas and bio-oil was found to depend on the nature of feedstock as well as the process conditions viz., pyrolytic-temperature, retention time and presence of additive. Sand additive improved the H2 composition while pyrolytic temperature increment caused a decline in CO2 fraction. Bio-char productivity increased with increasing temperature specifically with LEMA. Integration of thermo-chemical process with microalgae cultivation showed to yield multiple resources and accounts for environmental sustainability in the bio-refinery framework.