RESUMO
This study indicates that the immunobiology of macroscopically normal peritoneum is relevant to understand the pathogenesis of endometriosis. Peritoneal interleukin 6, interleukin 12, and ferritin were differentially expressed in women with and without endometriosis.
Assuntos
Endometriose/etiologia , Endometriose/patologia , Perfilação da Expressão Gênica , Peritônio/imunologia , Peritônio/patologia , Endometriose/fisiopatologia , Feminino , Ferritinas/genética , Expressão Gênica/imunologia , Humanos , Interleucina-12/genética , Interleucina-6/genética , Ciclo Menstrual/fisiologia , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Exposure of insulin-secreting ß-cells to inflammatory cytokines or high concentrations of free fatty acids, factors involved in the pathogenesis of type 1 and type 2 diabetes, leads to endoplasmic reticulum (ER) stress, ß-cell dysfunction, and eventually apoptotic ß-cell death. The aim of this study was to investigate the impact of ER stress on ß-cells at the protein level to evaluate the contribution of post-transcriptional and post-translational changes in ER stress-induced ß-cell damage. INS-1E cells were exposed in vitro to the ER-stress inducer cyclopiazonic acid (CPA) at two concentrations, and protein changes were evaluated using 2D-DIGE. CPA, 25 µM, led to massive apoptosis, accompanied by a near complete protein translation shut-down. CPA, 6.25 µM, led to adaptation of the ß-cells to ER stress. Identification of the differentially expressed proteins in the two conditions led to the discovery of a clear pattern of defense pathways, with post-translational modifications playing a crucial role. Key alterations included inhibition of insulin translation and post-translational modifications in ER chaperones HYOU1 and HSPA5. Also, a central role for 14-3-3 proteins is suggested. In conclusion, INS-1E cells are highly sensitive to ER stress, leading to important post-transcriptional and post-translational modifications that may contribute to ß-cell dysfunction and death.