RESUMO
Salmonella typhi and Helicobacter infections have been shown to increase risk of gallbladder cancer (GBC), but findings have been inconsistent. Other bacterial infections may also be associated with GBC. However, information on microbial pathogens in gallbladder bile of GBC patients is scarce. We aimed to investigate the microbial communities in gallbladder bile of patients with GBC and cholelithiasis (CL). Seven GBC patients and 30 CL patients were enrolled in this study. Genomic DNA was extracted from bile and the V3-V4 region of 16S rRNA was amplified. The sequencing results were compared with the 16S database, and the bacteria were identified by homology searches and phylogenetic analysis. DNA was detected in the bile of three GBC (42.9%; Bolivia, 1; Chile, 2) and four CL patients (13.3%; Bolivia, 1; Chile, 3). Of the 37 patients, 30 (81.1%) were negative and unable to analyze. Salmonella typhi and Helicobacter sp. were not detected in bile from any GBC patients. As the predominant species, Fusobacterium nucleatum, Escherichia coli, and Enetrobacter sp. were detected in bile from GBC patients. Those in bile from CL patients were Escherichia coli, Salmonella sp., and Enerococcus gallinarum. Escherichia coli was detected in bile samples from both GBC and CL patients. Whether the bacteria detected in bile from GBC patients would associated with the development of GBC warrant further investigation.
Assuntos
Bactérias/genética , Infecções Bacterianas/complicações , Bile/metabolismo , Colelitíase/etiologia , Neoplasias da Vesícula Biliar/etiologia , Vesícula Biliar/metabolismo , Metagenômica , Idoso , Bactérias/classificação , Bactérias/isolamento & purificação , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/microbiologia , Bile/microbiologia , Estudos de Casos e Controles , Colelitíase/patologia , Feminino , Seguimentos , Vesícula Biliar/microbiologia , Neoplasias da Vesícula Biliar/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , RNA Ribossômico 16S/genéticaAssuntos
Ductos Biliares/cirurgia , Colestase Extra-Hepática/cirurgia , Obstrução da Saída Gástrica/cirurgia , Gastroenterostomia/métodos , Jejuno/cirurgia , Estômago/cirurgia , Colestase Extra-Hepática/etiologia , Endossonografia , Derivação Gástrica/métodos , Obstrução da Saída Gástrica/etiologia , Humanos , Masculino , Pessoa de Meia-Idade , Cuidados Paliativos/métodos , StentsRESUMO
It is presented a 48 year-old male patient with antecedent of a foreign body intake (a beer metallic top) 4 years ago, who rejected surgery at first previous a failed attempt of endoscopic extraction. Four years later he was admitted in the Thorax National Institute in an emergency, with acute respiratory failure and sepsis signs that were corrected quickly; later on after failed surgical attempts of trachea-esophageal fistula correction and extraction of the foreign body, the patient was sent to BJIG where the endoscopic extraction of the foreign body located in the trachea was carried out together with the correction of the trachea-esophageal fistula by means of implantation, in esophagus, of covered self-expanding prosthesis.