RESUMO
Evidence from certain geographical areas links lymphomas of the ocular adnexa marginal zone B-cell lymphomas (OAMZL) with Chlamydophila psittaci (Cp) infection, suggesting that lymphoma development is dependent upon chronic stimulation by persistent infections. Notwithstanding that, the actual immunopathogenetical mechanisms have not yet been elucidated. As in other B-cell lymphomas, insight into this issue, especially with regard to potential selecting ligands, could be provided by analysis of the immunoglobulin (IG) receptors of the malignant clones. To this end, we studied the molecular features of IGs in 44 patients with OAMZL (40% Cp-positive), identifying features suggestive of a pathogenic mechanism of autoreactivity. Herein, we show that lymphoma cells express a distinctive IG repertoire, with electropositive antigen (Ag)-binding sites, reminiscent of autoantibodies (auto-Abs) recognizing DNA. Additionally, five (11%) cases of OAMZL expressed IGs homologous with autoreactive Abs or IGs of patients with chronic lymphocytic leukemia, a disease known for the expression of autoreactive IGs by neoplastic cells. In contrast, no similarity with known anti-Chlamydophila Abs was found. Taken together, these results strongly indicate that OAMZL may originate from B cells selected for their capability to bind Ags and, in particular, auto-Ags. In OAMZL associated with Cp infection, the pathogen likely acts indirectly on the malignant B cells, promoting the development of an inflammatory milieu, where auto-Ags could be exposed and presented, driving proliferation and expansion of self-reactive B cells.
Assuntos
Autoantígenos/imunologia , Neoplasias Oculares/imunologia , Genes de Imunoglobulinas , Linfoma de Zona Marginal Tipo Células B/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Análise por Conglomerados , Regiões Determinantes de Complementaridade , Neoplasias Oculares/etiologia , Feminino , Humanos , Cadeias Pesadas de Imunoglobulinas/genética , Região Variável de Imunoglobulina/genética , Ativação Linfocitária , Linfoma de Zona Marginal Tipo Células B/etiologia , Masculino , Pessoa de Meia-Idade , Psitacose/complicaçõesAssuntos
Linfoma de Células B/microbiologia , Linfoma Cutâneo de Células T/microbiologia , Psitacose/complicações , Neoplasias Cutâneas/microbiologia , Chlamydophila psittaci/genética , DNA Bacteriano/isolamento & purificação , Humanos , Reação em Cadeia da Polimerase Multiplex , Estudos RetrospectivosRESUMO
INTRODUCTION: We report an occasional biopsy of primary mucinous adenocarcinoma of the prostate with review of the literature and discussion about all criteria used to classify this clinical-pathological entity. MATERIALS AND METHODS: Histochemical (Alcian Blue and P.A.S.) and immunohistochemical (P.A.P. and P.S.A.) stainings were performed.