RESUMO
Previously, it was isolated from the fruiting bodies of the gilled mushroom Pholiota spumosa (Basidiomycetes, Strophariaceae), putrescine-1,4-dicinnamide, a phenylpropanoid derivative conjugated with polyamine putrescine never isolated before as a natural compound. Recently, polyamine analogs that are similar in structure to the natural polyamines but that cannot mimic their functions that are essential for cellular growth and differentiation, have shown antitumor activity in several types of human cancer cells. Therefore, we have now investigated the response of DU-145 cells, a well characterized androgen-independent human prostate cancer (PCA) cell line, to this phenylpropanoid derivative. The results presented here demonstrate that putrescine-1,4-dicinnamide, as suggested for polyamine analogs synthesized artificially, inhibits the cell growth of cancer cells inducing apoptosis cell death, mediated, at least in part, by the activation of caspase cascades, that at higher doses shift to necrosis, through the increase of reactive oxygen species (ROS) generation.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Basidiomycota , Proliferação de Células/efeitos dos fármacos , Fitoterapia , Putrescina/análogos & derivados , Antineoplásicos Fitogênicos/administração & dosagem , Antineoplásicos Fitogênicos/uso terapêutico , Linhagem Celular Tumoral/efeitos dos fármacos , Linhagem Celular Tumoral/metabolismo , Carpóforos , Humanos , Masculino , Neoplasias da Próstata/tratamento farmacológico , Neoplasias da Próstata/patologia , Putrescina/administração & dosagem , Putrescina/farmacologia , Putrescina/uso terapêuticoRESUMO
2-Geranylgeranyl-1,4-dihydroxybenzene [1] and a mixture of fatty acid esters of this phenol were isolated from the fruiting bodies of Lactarius lignyotus. Compound 1 was highly active in the brine shrimp test and showed significant inhibitory activity on DNA, RNA, and protein synthesis in HeLa and HL-60 cell lines.
Assuntos
Basidiomycota/química , Diterpenos , Terpenos/isolamento & purificação , DNA de Neoplasias/biossíntese , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Humanos , Leucina/metabolismo , Proteínas de Neoplasias/biossíntese , RNA Neoplásico/biossíntese , Terpenos/química , Terpenos/farmacologia , Timidina/metabolismo , Células Tumorais Cultivadas , Uridina/metabolismoRESUMO
This study of lipids of Lactarius uvidus has revealed the presence of new fatty acid esters of uvidin A [2a-d] and drimenol [6a-d]. Treatment of uvidin A [1] with base induced a Favorskii-like rearrangement leading to compounds with a new sesquiterpenoid skeleton. Uvidin A [1] showed insect antifeedant and cytotoxic activities.
Assuntos
Basidiomycota/química , Ácidos Graxos/isolamento & purificação , Sesquiterpenos/isolamento & purificação , Animais , Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Artemia , Ensaios de Seleção de Medicamentos Antitumorais , Ésteres , Ácidos Graxos/farmacologia , Comportamento Alimentar/efeitos dos fármacos , Humanos , Insetos , Sesquiterpenos/farmacologia , Células Tumorais CultivadasRESUMO
The composition of the essential oil of Aframomum giganteum was investigated by GC-MS. Forty seven terpenes were identified, the oil contained a high percentage of linalool and linalyl acetate which are mainly responsible for the smell of the A. giganteum stems. Fatty acids, paraffins, n-octanol, n-nonanol and guaiacol were also identified.