[GOAL-TARGET INFUSION THERAPY BASED ON NONINVASIVE HEMODYNAMIC MONITORING ESCOO].

We described our experience in using the new noninvasive hemodynamic monitoring (esCCO), which allowed recording most important parameters of heart capacity. Infusion therapy during laparoscopic operations might be based on dynamic of systolic blood volume. This functional approach can be used for evaluation of heart reaction on bolus fluid load, to optimike volemic status in situations associated with dynamical blood circulation changes. We compared intraoperative infused fluid volumes, calculated by traditional approach and by target approach. The obtained results had significant differences among groups, and didn't correspond with "liberal" and "restrictive" strategy for infusion therapy.

[Dexmedetomidine as a part of analgesic component of general anesthesia for laparoscopic operations].

UNLABELLED: The article deals with a study of efficacy and safety of the dexmedetomidine as an analgesic adjuvant drug for anaesthesia during laparoscopic gynecological and abdominal surgeries. Patients and methods: 32 patients received intraoperative infusion of dexmedetomidine. We assessed an effects of dexmedetomidine on data of central circulation during surgeries. CONCLUSIONS: The use of dexmedetomidine allows to decrease an amount of opioid analgesics, inhalation anaesthetics and to avoid severe changes of circulation during traumatic phases of surgeries.

Extensive changes in the transcriptional profile of human adipose tissue including genes involved in oxidative phosphorylation after a 6-month exercise intervention.

AIM: Adipose tissue has an important function in total energy homeostasis, and its dysregulation may contribute to lifestyle-related diseases such as type 2 diabetes, cancer and cardiovascular diseases. The aim of this study was to investigate genome-wide mRNA expression in adipose tissue in healthy men before and after an exercise intervention to identify genes or pathways that mediate the beneficial effect of regular exercise. We also investigated the difference in adipose tissue mRNA expression between individuals with or without a family history of type 2 diabetes. METHODS: The 6-month supervised exercise intervention was conducted in 47 healthy men (age 37.8 ± 4.3 years, BMI 28.5 ± 3.6 kg m(-2) ) with a previous low level of physical activity. RNA was analysed using GeneChip Human Gene 1.0 ST arrays (Affymetrix) before and after the exercise. RESULTS: We identified 2,560 significant transcripts differentially expressed before vs. after exercise with a false discovery rate (FDR) < 0.1%, including genes encoding the respiratory chain, histone subunits, small nucleolar RNAs and ribosomal proteins. Additionally, pathways enriched in response to exercise include the ribosome, oxidative phosphorylation, proteasome and many metabolic pathways, whereas the WNT and MAPK signalling pathways were down-regulated (FDR < 5%) after exercise. There were no significant differences in mRNA expression between individuals with or without a family history of type 2 diabetes. CONCLUSION: Exercise increased the expression of genes involved in oxidative phosphorylation, which is the opposite of what has been seen in adipose tissue from elderly or obese individuals with low physical fitness, and our study thereby demonstrates a mechanism for the beneficial effect of exercise.