RESUMO
The exacerbation of pneumonia in children with human adenovirus type 3 (HAdV-3E) is secondary to a Staphylococcus aureus (S. aureus) infection. The influence of host-pathogen interactions on disease progression remains unclear. It is important to note that S. aureus infections following an HAdV-3E infection are frequently observed in clinical settings, yet the underlying susceptibility mechanisms are not fully understood. This study utilized an A549 cell model to investigate secondary infection with S. aureus following an HAdV-3E infection. The findings suggest that HAdV-3E exacerbates the S. aureus infection by intensifying lung epithelial cell damage. The results highlight the role of HAdV-3E in enhancing the interferon signaling pathway through RIG-I (DDX58), resulting in the increased expression of interferon-stimulating factors like MX1, RSAD2, and USP18. The increase in interferon-stimulating factors inhibits the NF-κB and MAPK/P38 pro-inflammatory signaling pathways. These findings reveal new mechanisms of action for HAdV-3E and S. aureus in secondary infections, enhancing our comprehension of pathogenesis.
Assuntos
Infecções por Adenovirus Humanos , Adenovírus Humanos , Proteína DEAD-box 58 , Transdução de Sinais , Infecções Estafilocócicas , Staphylococcus aureus , Humanos , Células A549 , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Infecções por Adenovirus Humanos/metabolismo , Infecções por Adenovirus Humanos/imunologia , Infecções por Adenovirus Humanos/virologia , Adenovírus Humanos/fisiologia , Adenovírus Humanos/imunologia , Coinfecção/microbiologia , Proteína DEAD-box 58/metabolismo , Interações Hospedeiro-Patógeno/imunologia , Inflamação/metabolismo , NF-kappa B/metabolismo , Receptores Imunológicos/metabolismo , Infecções Estafilocócicas/imunologia , Infecções Estafilocócicas/metabolismo , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/patogenicidade , Ubiquitina TiolesteraseRESUMO
The aim of this study is to access influences of scan-position on clinical ultra-high-resolution CT scanning. We proposed a breath-hold assisted ultra-high-resolution scanning technology (scan scheme G) and compared with scan scheme A (regular CT plain scan) and scheme B (1024 ultra-high-resolution scan with patients stay in supine position). A total of 30 patients with fGGO were included in this study. Three highly experienced chest imaging doctors were employed to score the image and to select regions of interest (ROIs) for CT value and signal-to-noise ratio (SNR) calculation. In comparison with scan A and B, this new scan scheme G shows more clear CT images and higher SNRs at overall lung field (the p-values of A versus G and B versus G are 0.041 and 0.065, respectively). These findings suggest that scan-G provides a better image quality and contributes significantly to clinical detection accuracy of fGGO.