RESUMO
BACKGROUND AND OBJECTIVE: The primary reasons for the failure were recurrence and metastasis in the treatment of gastric cancer. This study was designed to investigate the distributive regularity of EGFR, IL-6R, PCNA, DI and the diploid in the gastric cancer patients with recurrence and metastasis, to provide reliable basis to judge whether it has metastasis before operation and recurrence post operation. METHOD: Immunohistochemistry SP method and image analyzer (Fegulgen staining) were used to test the EGFR, IL-6R, PCNA, DI and the diploid in 199 patients with gastric cancer, 20 cases with normal gastric mucous membrane, and 20 cases of atypical hyperplasia. RESULTS: The level of expression of EGFR was higher in the patients with metastasis and residual gastric cancer(61.11%, 66%, 66.67%) than in the patients with other cancers, normal gastric mucous membrane, atypical hyperplasia(47.83%, 0%, 35%) (P < 0.01); In the distribution form of DNA diploid, the rate of non-diploid cells was remarkably higher in the patients with metastasis and residual gastric cancer(56.9 +/- 13.3% vs 12.8 +/- 6.3%, 0%, 12.2), and so is the DNA index (2.91 +/- 0.33 vs 2.17 +/- 0.29), but the rate of 2C cells is lower(43.8 +/- 12.9 vs 10.2 +/- 7.5, P < 0.01). CONCLUSION: EGFR, DI, and the diploid are valuable targets for judging metastasis and recurrence of gastric cancer before and after operation.