RESUMO
MicroRNAs (miRNAs) have been identified as potential biomarkers for endometrial carcinoma (EC) diagnosis, prognosis and therapy. The purpose of the present study was to investigate the detailed role and molecular mechanism of miR-195 in EC metastasis. qRT-PCR assay was performed to assess the expression of miR-195 and SRY-related high-mobility group box 4 (SOX4) mRNA in EC tissues and cells. The levels of N-cadherin, Vimentin, E-cadherin and SOX4 protein were determined by western blot. SOX4 protein expression in EC tissues was also determined by Immunohistochemistry (IHC) experiment. Transwell assay was used to analyze cell migration and invasion abilities. Dual-luciferase reporter assay and RNA Immunoprecipitation (RIP) assay were performed to confirm the targeted interaction between miR-195 and SOX4. Our data supported that miR-195 was downregulated and SOX4 was upregulated in EC tissues and cell lines. Upregulation of miR-195 inhibited migration, invasion and epithelial-mesenchymal transition (EMT) of EC cells. Moreover, SOX4 was a direct target of miR-195. MiR-195 overexpression-mediated anti-migration, anti-invasion and anti-EMT effects were antagonized by SOX4 restoration in EC cells. In conclusion, our study suggested that miR-195 inhibited the migration, invasion and epithelial mesenchymal transition (EMT) of EC cells at least partly by targeting SOX4. Our study provided a novel underlying mechanism for EC metastasis and a promising therapeutic target for EC management.
Assuntos
Neoplasias do Endométrio/genética , Transição Epitelial-Mesenquimal/genética , MicroRNAs/genética , Fatores de Transcrição SOXC/genética , Idoso , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Neoplasias do Endométrio/patologia , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Pessoa de Meia-Idade , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , RNA Mensageiro/genéticaRESUMO
Primary carcinoma of the renal calyx is extremely rare. The present study reported nephron sparing endoscopic treatment for primary carcinoma of the renal calyx. An 81-year-old female presented with a 1-year history of intermittent painless gross hematuria. Computed tomography and X-ray of the urinary tract were unable to definitively identify any lesion. Flexible ureteroscopic examination revealed a tumor with epicenter in the lower calyx of the right kidney, with additional involvement around the calyx. Biopsies were obtained and pathology revealed low-grade urothelial carcinoma. Considering additional co-morbidities, the patient elected to undergo endoscopic management with thulium laser. The present report described the feasibility of flexible ureteroscopic thulium laser resection for the treatment of renal calyx carcinoma.
RESUMO
Malignant mesothelioma is a rare type of cancer, most commonly associated with exposure to asbestos. Mesothelioma of the peritoneum, the membrane lining the abdominal cavity, is extremely rare. The current study reports the case of a 60-year-old female who presented with intestinal fistula, recurrent incisional hernia and abdominal infection, with no history of asbestos exposure, and was diagnosed with clear cell MPM. Computed tomography scans of the abdomen revealed extensive small bowel adhesions and massive peritoneal effusion. Histological examination of biopsy specimens indicated a diagnosis of malignant peritoneal mesothelioma with clear cell morphology. A laparotomy was performed, with subsequent resection of the bowel with fistula. Follow-up examination performed at 1-year post-surgery revealed that the patient was alive and in generally good health.