RESUMO
INTRODUCTION: A family history of psychiatric disorders is one of the strongest risk factors for schizophrenia. The characteristics of patients with a family history of psychiatric disorders have not been systematically evaluated. METHODS: This multicenter study (26 centers, 2425 cases) was performed in a Chinese population to examine the sociodemographic and clinical characteristics of schizophrenia patients with a family history of psychotic disorders in comparison with those of patients with sporadic schizophrenia. RESULTS: Nineteen percent of patients had a family history of mental disease. Multiple logistic regression analysis revealed that ≥4 hospitalizations (OR = 1.78, P = .004), tobacco dependence (OR = 1.48, P = .006), alcohol dependence (OR = 1.74, P = .013), and physical illness (OR = 1.89, P = .001) were independently and significantly associated with a family history of mental disease. CONCLUSION: Patients with a family history of mental disorders present different demographics and clinical features than patients without a family history of psychiatric disorders.
Assuntos
Transtornos Psicóticos , Esquizofrenia , Hospitalização , Humanos , Transtornos Psicóticos/epidemiologia , Transtornos Psicóticos/genética , Fatores de Risco , Esquizofrenia/epidemiologia , Esquizofrenia/genéticaRESUMO
MicroRNA (miR)200b3p is downregulated in multiple human cancer types. Wnt signaling serves a role in human colorectal cancer (CRC). The present study aimed to examine the effect of miR200b3p on human CRC and its potential association with Wnt signaling. The Cell Counting Kit8 (CCK8) was employed to assess cell viability. A flow cytometric assay was conducted to examine cell proliferation and apoptosis. The regulation model of miR200b3p and Wnt1 was assessed by a luciferase reporter assay. A commercial kit was used to evaluate the activity of caspase3 following treatment of the cells by miR200b3p or Wnt1. The expression of target factors was determined by a quantitative realtime polymerase chain reaction and western blot analysis. The expression of miR200b3p was decreased in human CRC tissues and in cell lines. The bioinformatics analysis and the luciferase reporter assay revealed that Wnt1 may be a direct target of miR200b3p. Moreover, the viability and proliferation of CRC cells was suppressed by miR200b3p. miR200b3p additionally induced apoptosis in CRC cells. Furthermore, the caspase3 activity was enhanced in the miR200b3p mimics group. The expression of antigen Ki67 (additionally termed KI67) and ßcatenin was decreased, while the expression of cleaved caspase3 was increased by miR200b3p. In conclusion, miR200b3p inhibited proliferation and induced apoptosis in CRC cells by inactivating Wnt/ßcatenin signaling. The present study provided potential biomarkers and candidate modalities for the management of CRC.
Assuntos
Apoptose , Proliferação de Células , Neoplasias Colorretais/patologia , MicroRNAs/metabolismo , Proteína Wnt1/metabolismo , Regiões 3' não Traduzidas , Adulto , Idoso , Antagomirs/metabolismo , Caspase 3/genética , Caspase 3/metabolismo , Linhagem Celular Tumoral , Neoplasias Colorretais/metabolismo , Feminino , Humanos , Antígeno Ki-67/genética , Antígeno Ki-67/metabolismo , Masculino , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Pessoa de Meia-Idade , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Via de Sinalização Wnt , Proteína Wnt1/antagonistas & inibidores , Proteína Wnt1/genética , beta Catenina/genética , beta Catenina/metabolismoRESUMO
BACKGROUND & AIMS: Patients with irritable bowel syndrome (IBS) often have psychiatric comorbidities. Alterations in the intestinal microbiota have been associated with IBS and depression, but it is not clear if there is a microbial relationship between these disorders. We studied the profiles of fecal microbiota samples from patients with IBS, depression, or comorbidities of IBS and depression; we determined the relationships among these profiles and clinical and pathophysiological features of these disorders. METHODS: We used 454 pyrosequencing to analyze fecal microbiota samples from 100 subjects (40 with diarrhea-predominant IBS [IBS-D], 15 with depression, 25 with comorbidities of IBS and depression, and 20 healthy individuals [controls]), recruited at Peking University. Abdominal and psychological symptoms were evaluated with validated questionnaires. Visceral sensitivity was evaluated using a barostat. Colonic mucosal inflammation was assayed by immunohistochemical analyses of sigmoid tissue biopsy specimens. RESULTS: Fecal microbiota signatures were similar between patients with IBS-D and depression in that they were less diverse than samples from controls and had similar abundances of alterations. They were characterized by high proportions of Bacteroides (type I), Prevotella (type II), or nondominant microbiota (type III). Most patients with IBS-D or depression had type I or type II profiles (IBS-D had 85% type I and type II profiles, depression had 80% type I and type II profiles). Colon tissues from patients with type I or type II profiles had higher levels of inflammatory markers than colon tissues from patients with type III profiles. The level of colon inflammation correlated with the severity of IBS symptoms. CONCLUSIONS: Patients with IBS-D and depression have similar alterations in fecal microbiota; these might be related to the pathogenesis of these disorders. We identified 3 microbial profiles in patients that could indicate different subtypes of IBS and depression or be used as diagnostic biomarkers.