[Color characterization and its correlation with quality index during ripening of Rubus chingii].

The color of Rubus chingii was characterized by digital method, and the content of water extract, alcohol extract, total flavonoids, total polysaccharides, total polyphenols, ellagic acid, linden glycoside, kaophenol-3-O-rutin were determined. Correlation regression was used to analyze the correlation between color and composition. The results showed that L~* was positively correlated with total polyphenols, kaophenol-3-O-rutin and tilide, and moderately positively correlated with total flavones, ellagic acid and aqueous extracts. The a~* value was negatively correlated with total polyphenols, kaophenol-3-O-rutin, and linden glycosides, while was moderately correlated with total flavones, aqueous extracts, and ellagic acid. The b~* value was negatively correlated with the water extract, and moderately correlated with the content of total polyphenols, total polysaccharides, alcohol extract and kaophenol-3-O-rutin, which showed that R. chingii mature color had a significant correlation with material composition in the process of dynamic change. According to the law of dynamic change in the color and quality indexes, it is determined that the appropriate harvest time is in late April to May 1, while the fruit is not turn yellow. The agronomic traits related to fruit was(12.49±0.56) mm in diameter,(14.25±1.19)mm in height,(1.20±0.14) g in weight, the chroma L~* value was 52.87±3.14,a~* value was 2.01±1.58, b~* values was 28.31±3.88. The results lay a foundation for establishing an objective quantitative evaluation model of R. chingii color from experience.

Isaria cicadae conidia possess antiproliferative and inducing apoptosis properties in gynaecological carcinoma cells.

Isaria cicadae is an entomogenous fungus that has been used as a traditional Chinese medicinal materials to treat different diseases, including cancer. However, Isaria cicadae conidia for inhibitory activity against breast cancer cells growth are still not systematically studied. The present aim was to elucidate the phytochemical composition of Isaria cicadae conidia and to explore relevant anti-cancer potential in gynaecological carcinoma MCF-7 and Hela cells. Isaria cicadae conidia were identified by UPLC-ESI-Q-TOF-MS: high performance liquid chromatography-electrospray/quadrupole time of flight tandem mass spectrometry technology. Eight main compounds were identified which are nucleosides, cordycepic acid, cordycepin, beauvericin and myriocin by MS fragmentation ions. The nuclear morphology indicated the typical characteristics of apoptosis by Hoechst staining. Annexin V/PI staining revealed that the number of apoptotic cells was increased by Isaria cicadae conidia treatment. Furthermore, Isaria cicadae conidia also induced the caspase-mediated mitochondrial apoptosis pathway. The findings suggest that the full-scale active ingredients highlight the significance of Isaria cicadae conidia as potential anti-cancer agent in China.

Influence of ribosomal protein L39-L in the drug resistance mechanisms of lacrimal gland adenoid cystic carcinoma cells.

BACKGROUND: Cancer constitutes a key pressure on public health regardless of the economy state in different countries. As a kind of highly malignant epithelial tumor, lacrimal gland adenoid cystic carcinoma can occur in any part of the body, such as salivary gland, submandibular gland, trachea, lung, breast, skin and lacrimal gland. Chemotherapy is one of the key treatment techniques, but drug resistance, especially MDR, seriously blunts its effects. As an element of the 60S large ribosomal subunit, the ribosomal protein L39-L gene appears to be documented specifically in the human testis and many human cancer samples of different origins. MATERIALS AND METHODS: Total RNA of cultured drug-resistant and susceptible lacrimal gland adenoid cystic carcinoma cells was seperated, and real time quantitative RT-PCR were used to reveal transcription differences between amycin resistant and susceptible strains of lacrimal gland adenoid cystic carcinoma cells. Viability assays were used to present the amycin resistance difference in a RPL39-L transfected lacrimal gland adenoid cystic carcinoma cell line as compared to control vector and null-transfected lacrimal gland adenoid cystic carcinoma cell lines. RESULTS: The ribosomal protein L39-L transcription level was 6.5-fold higher in the drug-resistant human lacrimal gland adenoid cystic carcinoma cell line than in the susceptible cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells revealed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. CONCLUSIONS: The ribosomal protein L39-L gene could possibly have influence on the drug resistance mechanism of lacrimal gland adenoid cystic carcinoma cells.

[Factors influence volatile oil content in Ocimun basilicum introduced from Xinjiang province to south-east China].

OBJECTIVE: To obtain the information on ecological adaptation of Ocimun basilicum introduced from Xinjiang to Hangzhou and study the effect of different harvesting times, drying methods, and different organs of Ocimun basilicum on Volatile oil content METHODS: Extraction was undertaken according to The Pharmacopoeia of China, 2010 edition. RESULTS: Sun-drying was the most efficient way to obtain Volatile oil compared with other methods. The largest biomass was harvested at 3rd, September. Furthermore, Volatile oil was found to accumulate mostly in the flowers and little in the stems. CONCLUSION: Ocimun basilicum can readily inhabit in Hangzhou and its economic value can be significant improved by growing two seasons per year. Only harvest leaves and flowers can significantly reduce the cost for transport and also increase oil extract rate of Volatile oil.

[GC-MS analysis of essential oil from anthodiums of Chrysanthemum morifolium processed by microwave-airflow and steam calefaction].

OBJECTIVE: Analysis of the constituents of the essential oil extracted from the flowers of the Chrysanthemum morifolium processed by the microwave-airflow and the calefaction after steam process from the State Chrysanthemum GAP bases in Zhejiang Province and to provide scientific basis for quality control. METHOD: The essential oil was extracted by water-steam distillation and separated by GC capillary column chromatography. The components were quantitatively determined with normalization method, and identified by GC-MS. RESULT: From the microwave-airflow dried sample and the calefactively dried sample after steam process, 119 and 175 components were detected, among them fifty and fifty-five components were identified, which were composed of 67.89% and 63.64% of the total essential oil, respectively. CONCLUSION: The yield of the essential oil extracted from the microwave-airflow dried sample was 0.40%, and that from the calefactively dried sample after steam process was 0. 19%. The former is 2.1 times higher than the latter. The components of the essential oil are similar and there are differences between the content of corresponding compounds in the two samples. The results showed that application of microwave-airflow combined drying technology remained original and essential constituents. The production benefit was improved greatly. The grade and quality of C. morifolium enhanced, and it's market selling price was increased compared to the traditional drying calefaction after steam process.