The effect of rice protein-polyphenols covalent and non-covalent interactions on the structure, functionality and in vitro digestion properties of rice protein.

The characteristics of the crosslinking between rice protein (RP) and ferulic acid (FA), gallic acid (GA), or tannin acid (TA) by covalent binding of Laccase and non-covalent binding were evaluated. The RP-polyphenol complexes greatly improved the functionality of RP. The covalent effect with higher polyphenol binding equivalence showed higher emulsion activity than the non-covalent effect. The solubility, and antioxidant activity of covalent binding were higher than that of non-covalent binding in the RP-FA group, but there was a contrasting behavior in the RP-GA group. The RP-FA was most soluble in conjugates, while the RP-GA had the highest solubility in mixtures. It was found that the covalent complexes were more stable in the intestinal tract. The content of polyphenols in the RP-TA group was rapidly increased at the later intestinal digestion, which indicated the high polyphenol-protective effect in this group. Meanwhile, the RP-TA group showed high reducing power but low digestibility.

Pulsed electric field improves the EGCG binding ability of pea protein isolate unraveled by multi-spectroscopy and computer simulation.

Understanding molecular mechanisms during protein modification is critical for expanding the application of plant proteins. This study investigated the conformational change and molecular mechanism of pea protein isolate (PPI) under pulsed electric field (PEF)-assisted (-)-Epigallocatechin-Gallate (EGCG) modification. The flexibility of PPI was significantly enhanced after PEF treatment (10 kV/cm) with decrease (23.25 %) in α-helix and increase (117.25 %) in random coil. The binding constant and sites of PEF-treated PPI with EGCG were increased by 2.35 times and 10.00 % (308 K), respectively. Molecular docking verified that PEF-treated PPI had more binding sites with EGCG (from 4 to 10). The number of amino acid residues involved in hydrophobic interactions in PEF-treated PPI-EGCG increased from 5 to 13. PEF-treated PPI-EGCG showed a significantly increased antioxidant activity compared to non-PEF-treated group. This work revealed the molecular level of PEF-assisted EGCG modification of PPI, which will be significant for the application of PPI in food industry.

High-mechanical strength carboxymethyl chitosan-based hydrogel film for antibacterial wound dressing.

Hydrogels, being highly biocompatible and adaptable with biological tissues, have shown great usability in biomedical applications. In this research, a novel hydrogel film developed from carboxymethyl chitosan (CMCS) loaded with waterborne polyurethane-gelatin hydrolysate was synthesized via aqueous emulsion copolymerization. The synthesized hydrogel film was characterized using mechanical strength tests, FTIR, XPS, SEM, AFM, and various other analysis technologies. The results demonstrated that the hydrogel film exhibited good thermal stability, swelling behavior, as well as controllable biodegradability. Specifically, when the CMCS content was loaded at 6 %, the maximum tensile strength and elongation at the break of the hydrogel film were reached 31.69 MPa and 447.187, respectively. The disk diffusion tests indicated that the hydrogel film presented significant antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). These results indicate that hydrogel films with high mechanical strength and high antibacterial activity could be used for wound dressing applications.

Characterisation of thermal and structural behaviour of lipid blends composed of fish oil and milkfat.

The blend of fish oil with a high percentage of long chain poly-unsaturated fatty acids, and milkfat with a high percentage of saturated fatty acids, could potentially demonstrate desirable characteristics from both components, such as increased omega-3 fatty acids and melting point, as well as improved crystallization and oxidative stability. In this study, the effect of various milkfat concentrations on thermal properties and crystalline structure of these blends were analysed to understand parameters determining the overall characteristics of the blend. Blends with different ratios of fish oil: milkfat (9:1, 7:3, 5:5, 3:7, 1:9), as well as pure fish oil and pure milkfat, were investigated at different cooling conditions. The crystallization behaviour in all samples shifted to lower temperature ranges, by increasing the cooling rate from 1 to 32 °C/min. However, the changes in cooling rate did not have significant effect on the melting profile of the samples. Whereas changes in milkfat ratio affect both the crystallization and melting behaviour. New crystallization peaks were observed on DSC spectra between the range of -4 to -13 °C in the blends. Moreover, new melting peaks appeared in two ranges of -1 to -8 °C and 8-9 °C, in the blends. The crystallization and melting behaviour of the blends were similar to those of milkfat when >30% milkfat was used. This was further confirmed via XRD where milkfat demonstrated the dominant polymorphic behaviour. Regarding shape of the crystals, fractal dimension analysis showed a similarity between clusters in blends containing 50% milkfat or higher. Increasing the ratio of milkfat led to an increase in fractal dimension which indicates higher mass-spatial distribution of the crystal networks in the blends. The data showed that adding 30% or more milkfat to pure fish oil resulted in blends demonstrating similar characteristics to milkfat, including thermal, structural, and oxidative stability. This shows the potential of blending a high percentage of docosahexaenoic acid in milk fat to improve their overall stability.