RESUMO
High oil and protein content make tetraploid peanut a leading oil and food legume. Here we report a high-quality peanut genome sequence, comprising 2.54 Gb with 20 pseudomolecules and 83,709 protein-coding gene models. We characterize gene functional groups implicated in seed size evolution, seed oil content, disease resistance and symbiotic nitrogen fixation. The peanut B subgenome has more genes and general expression dominance, temporally associated with long-terminal-repeat expansion in the A subgenome that also raises questions about the A-genome progenitor. The polyploid genome provided insights into the evolution of Arachis hypogaea and other legume chromosomes. Resequencing of 52 accessions suggests that independent domestications formed peanut ecotypes. Whereas 0.42-0.47 million years ago (Ma) polyploidy constrained genetic variation, the peanut genome sequence aids mapping and candidate-gene discovery for traits such as seed size and color, foliar disease resistance and others, also providing a cornerstone for functional genomics and peanut improvement.
Assuntos
Arachis/genética , Arachis/embriologia , Arachis/fisiologia , Mapeamento Cromossômico , Cromossomos de Plantas/genética , Resistência à Doença/genética , Domesticação , Secas , Ecótipo , Evolução Molecular , Genoma de Planta , Cariótipo , Óleo de Amendoim/metabolismo , Melhoramento Vegetal , Doenças das Plantas/prevenção & controle , Proteínas de Vegetais Comestíveis/metabolismo , Poliploidia , Sementes/anatomia & histologia , Sementes/genéticaAssuntos
Aminoácidos , Conformação Proteica em alfa-Hélice , Temperatura , Proteínas do Envelope Viral , Zika virus/química , Humanos , Microscopia Eletrônica/métodos , Proteínas do Envelope Viral/química , Proteínas do Envelope Viral/metabolismo , Zika virus/genética , Zika virus/fisiologia , Infecção por Zika virusRESUMO
Zika virus (ZIKV) has evolved into a global health threat because of its unexpected causal link to microcephaly. Phylogenetic analysis reveals that contemporary epidemic strains have accumulated multiple substitutions from their Asian ancestor. Here we show that a single serine-to-asparagine substitution [Ser139âAsn139 (S139N)] in the viral polyprotein substantially increased ZIKV infectivity in both human and mouse neural progenitor cells (NPCs) and led to more severe microcephaly in the mouse fetus, as well as higher mortality rates in neonatal mice. Evolutionary analysis indicates that the S139N substitution arose before the 2013 outbreak in French Polynesia and has been stably maintained during subsequent spread to the Americas. This functional adaption makes ZIKV more virulent to human NPCs, thus contributing to the increased incidence of microcephaly in recent ZIKV epidemics.
Assuntos
Microcefalia/virologia , Proteínas do Envelope Viral/genética , Infecção por Zika virus/virologia , Zika virus/genética , Zika virus/patogenicidade , América/epidemiologia , Substituição de Aminoácidos , Animais , Asparagina/genética , Linhagem Celular Tumoral , Cricetinae , Surtos de Doenças , Humanos , Incidência , Camundongos , Microcefalia/epidemiologia , Mutação , Células-Tronco Neurais/virologia , Polinésia/epidemiologia , Serina/genética , Infecção por Zika virus/complicações , Infecção por Zika virus/epidemiologiaRESUMO
Although several different flaviviruses may cause encephalitis, Japanese encephalitis virus is the most significant, being responsible for thousands of deaths each year in Asia. The structural and molecular basis of this encephalitis is not fully understood. Here, we report the cryo-electron microscopy structure of mature Japanese encephalitis virus at near-atomic resolution, which reveals an unusual "hole" on the surface, surrounded by five encephalitic-specific motifs implicated in receptor binding. Glu138 of E, which is highly conserved in encephalitic flaviviruses, maps onto one of these motifs and is essential for binding to neuroblastoma cells, with the E138K mutation abrogating the neurovirulence and neuroinvasiveness of Japanese encephalitis virus in mice. We also identify structural elements modulating viral stability, notably Gln264 of E, which, when replaced by His264 strengthens a hydrogen-bonding network, leading to a more stable virus. These studies unveil determinants of neurovirulence and stability in Japanese encephalitis virus, opening up new avenues for therapeutic interventions against neurotropic flaviviruses.Japanese encephalitis virus (JEV) is a Flavivirus responsible for thousands of deaths every year for which there are no specific anti-virals. Here, Wang et al. report the cryo-EM structure of mature JEV at near-atomic resolution and identify structural elements that modulate stability and virulence.