Traceable and Integrated Pesticide Screening (TIPS), a Systematic and Retrospective Strategy for Screening 900 Pesticides and Unknown Metabolites in Tea.

Pesticide management is a crucial issue for sustainable agriculture and food safety. The high-resolution mass spectrometry (HRMS)-based screening method has become a popular choice to monitor pesticide residues in foods and the environment. Data-independent acquisition (DIA) was the first option allowing for this type of analysis due to the wide compound coverage compared to traditional targeted analysis using triple-quadrupole tandem mass spectrometry (QqQ). However, a higher false-positive detection rate is a critical shortcoming in DIA. To overcome this concern, a rigorous method is needed to determine the reliable information acquired from DIA screening. A systematic strategy, traceable and integrated pesticide screening (TIPS), was proposed in this study to comprehensively monitor pesticides and metabolites in a complex tea matrix, avoiding false-positive detection. A total of 900 pesticides were added to an in-house database and evaluated through precision tests, which showed good repeatability and reproducibility. One hundred pesticides and metabolites were detected and confirmed by TIPS in 98 commercial tea samples. In addition to the authorized pesticides that could be detected in TIPS, chlorfluazuron, diafenthiuron, and tolfenpyrad, which are pesticides not allowed to be used in tea farming, were frequently found in this study. In addition, dinotefuran DN and fenbuconazole metabolites RH-9129 and/or RH-9130 were tentatively identified in the archived data using retrospective analysis. The HRMS-based data in TIPS could be a record platform for tracing novel or emerging contaminants not initially targeted in samples. TIPS, a novel strategy, has great potential for rapidly conducting a risk assessment of unexpected pesticides in food.

Investigating the Impact of Extruded Dehulled Adlay with Specific In Vitro Digestion Properties on Blood Lipids in Subjects with Mild to Moderate Dyslipidemia.

Dyslipidemia, a major risk factor for cardiovascular diseases (CVDs), is modifiable by diet and lifestyle changes. A large population with mild to moderate dyslipidemia is at risk of developing CVDs, and early initiation of preventive measures can avert advancing into severe medical conditions. Studies suggest increasing slowly digestible starch (SDS) in diets can help lower blood lipids. We processed dehulled adlay, a cereal rich in bioactive compounds, such as polyphenols and phytosterols, into an instant meal by extrusion and milling and then assessed its starch composition and in vitro digestibility. The dehulled adlay was found to consist of 32% SDS and resistant starch combined. Then, eligible subjects with dyslipidemia were recruited to explore the adlay's hypolipidemic potential, safety, and acceptability. Subjects consumed the dehulled adlay as the sole carbohydrate source in their breakfast, without changing other components in the diet or lifestyle, for 12 weeks. After intervention, serum total cholesterol (TC) decreased significantly in subjects with hypercholesterolemia. In addition, both TC and triglyceride levels decreased significantly in those above 50 years old. In conclusion, the extruded dehulled adlay displays potential for favorably modulating blood lipids, and the effect is more pronounced in the middle-aged population.

Djulis (Chenopodium Formosanum) Prevents Colon Carcinogenesis via Regulating Antioxidative and Apoptotic Pathways in Rats.

Djulis is a cereal crop rich in polyphenols and dietary fiber that may have nutraceutical activity to prevent colon cancer. This study was designed to examine the preventive effect of djulis on colon carcinogenesis in rats treated with 1,2-dimethylhydrazine (DMH). Rats were fed different AIN-93G-based diets: groups N and DMH were fed AIN-93G diet and groups LD, MD, and HD were fed AIN-93G diet containing 5, 10, and 20% djulis, respectively. All rats except for group N were injected with DMH to induce colon carcinogenesis. After 10 weeks, rats were sacrificed and colon and liver tissues were collected for analysis. The results showed that djulis-treated rats had significantly lower numbers of colonic preneoplastic lesions, aberrant crypt foci (ACF), sialomucin-producing (SIM)-ACF, and mucin-depleted foci. Djulis treatment increased superoxide dismutase and catalase activities in colon and liver. Djulis also reduced p53, Bcl-2, and proliferating cell nuclear antigen expressions and increased Bax and caspase-9 expressions. Besides, phenolic compounds and flavonoids were found rich in djulis. These results demonstrate the chemopreventive effect of djulis on carcinogen-induced colon carcinogenesis via regulating antioxidative and apoptotic pathways in rats. Djulis may have the potential to be developed as a valuable cereal product for chemoprevention of colon cancer.