RESUMO
Thymoma is one of the thymic epithelial tumors arising from the thymic epithelial cells of a variety of animal species including cat, cattle, dog, goat, horse, and pig. The cancer cells of thymoma are always localized within thymus. Feline thymoma was usually identified in the cranial mediastinum of elder cats. In this report, we present the full diagnostic characteristics of a young cat with a cranial mediastinal mass diagnosed as a cystic type B1 thymoma. A 3-year-old male neutered domestic short-haired cat was referred for further diagnosis due to the shortness of breath and pleural accumulation. One single cystic extra-pericardial mass was seen and the boundary between the mass and the surrounding tissue was clearly demarcated in three-view thoracic radiographs. Ultrasound showed pleural effusion and intra-thoracic mass. Pleural fluid biochemistry showed high level of triglycerides and complete blood count revealed the elevated number of basophils and high level of feline serum amyloid A (fSAA). Pleural effusion sediment cytology showed a large number of round cells, mainly small lymphocytes, as well as a moderate number of neutrophils and a small number of large lymphocytes. The high level of triglycerides in pleural fluid instead of serum and high level of fSAA seem to provide informative clues to the diagnosis of thymoma and are worthy of further investigation.
RESUMO
In the tobacco industry, impurity detection is an important prerequisite for ensuring the quality of tobacco. However, in the actual production process, the complex background environment and the variability of impurity shapes can affect the accuracy of impurity detection by tobacco robots, which leads to a decrease in product quality and an increase in health risks. To address this problem, we propose a new online detection method of tobacco impurities for tobacco robot. Firstly, a BCFormer attention mechanism module is designed to effectively mitigate the interference of irrelevant information in the image and improve the network's ability to identify regions of interest. Secondly, a Dual Feature Aggregation (DFA) module is designed and added to Neck to improve the accuracy of tobacco impurities detection by augmenting the fused feature maps with deep semantic and surface location data. Finally, to address the problem that the traditional loss function cannot accurately reflect the distance between two bounding boxes, this paper proposes an optimized loss function to more accurately assess the quality of the bounding boxes. To evaluate the effectiveness of the algorithm, this paper creates a dataset specifically designed to detect tobacco impurities. Experimental results show that the algorithm performs well in identifying tobacco impurities. Our algorithm improved the mAP value by about 3.01% compared to the traditional YOLOX method. The real-time processing efficiency of the model is as high as 41 frames per second, which makes it ideal for automated inspection of tobacco production lines and effectively solves the problem of tobacco impurity detection.
RESUMO
Gemcitabine (GEM) resistance affects chemotherapy efficacy of pancreatic cancer (PC). Cancer-associated fibroblasts (CAFs) possess the ability of regulating chemoresistance. This study probed the mechanism of hypoxia-treated CAFs regulating cell stemness and GEM resistance in PC. Miapaca-2/SW1990 were co-cultured with PC-derived CAFs under normoxic/hypoxic conditions. Cell viability/self-renewal ability was determined by MTT/sphere formation assays, respectively. Protein levels of CD44, CD133, Oct4, and Sox2 were determined by western blot. GEM tumoricidal assay was performed. PC cell GEM resistance was evaluated by MTT assay. CAFs were cultured at normoxia/hypoxia. HIF-1α and miR-21 expression levels were assessed by RT-qPCR and western blot, with their binding sites and binding relationship predicted and verified. CAF-extracellular vesicles (EVs) were incubated with Miapaca-2 cells. The RAS/AKT/ERK pathway activation was detected by western blot. PC xenograft models were established and treated with hypoxic CAF-EVs and GEM. CAFs and PC cell co-culture increased cell stemness maintenance, GEM resistance, cell viability, stem cell sphere number, and protein levels of CD44, CD133, Oct4, and Sox2, and weakened GEM tumoricidal ability to PC cells, with the effects further enhanced by hypoxia. Hypoxia induced HIF-1α and miR-21 overexpression in CAFs. Hypoxia promoted CAFs to secrete high-level miR-21 EVs via the HIF-1α/miR-21 axis, and activated the miR-21/RAS/AKT/ERK pathway. CAF-EVs promoted GEM resistance in PC via the miR-21/RAS/ATK/ERK pathway in vivo. Hypoxia promoted CAFs to secrete high-level miR-21 EVs through the HIF-1α/miR-21 axis, and activated the miR-21/RAS/AKT/ERK pathway via EVs to trigger stemness maintenance and GEM resistance in PC.
Assuntos
Fibroblastos Associados a Câncer , MicroRNAs , Neoplasias Pancreáticas , Humanos , Gencitabina , Fibroblastos Associados a Câncer/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Linhagem Celular Tumoral , Hipóxia/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Neoplasias Pancreáticas/tratamento farmacológico , Neoplasias Pancreáticas/metabolismoRESUMO
Given the increasing incidence of pancreatic cancer and the low survival rate, the exploration of the complex tumor microenvironment and the development of novel treatment options is urgent. NK cells, known for their cytotoxic abilities and modulation of other immune cells, are vital in recognizing and killing cancer cells. However, hypoxic conditions in the tumor microenvironment have been found to impair NK cell functionality and contribute to tumor immune escape. Therefore, we aimed to uncover the mechanism through which hypoxia mediates the immune escape of pancreatic cancer cells, focusing on the influence of miR-1275/AXIN2 on NK cells. Using a combination of GEO dataset screening, Tumor Immune Estimation Resource 2.0 immunoscore screening, and the Cancer Genome Atlas data, we identified a correlation between miR-1275 and NK cells. The down-regulation of miR-1275 was associated with decreased NK cell activity and survival in patients with pancreatic cancer. Pathway analysis further linked miR-1275 expression with the hypoxic HIF1A pathway. In vitro experiments were conducted using the NK-92 cell, revealing that hypoxia significantly reduced miR-1275 expression and correspondingly decreased the cell-killing ability of NK cells. Upregulation of miR-1275 increased perforin, IFN-γ and TNF-α expression levels and enhanced NK cell cytotoxicity. Additionally, miR-1275 was found to bind to and inhibit AXIN2 expression, which when overexpressed, partially alleviated the promotive effect of upregulated miR-1275 on NK-92 cell killing ability. In conclusion, this research underscores the critical role of the miR-1275/AXIN2 axis in hypoxia-mediated immune escape in pancreatic cancer, thus opening new potential avenues for treatment strategies.
Assuntos
MicroRNAs , Neoplasias Pancreáticas , Humanos , Células Matadoras Naturais , Hipóxia/genética , Hipóxia/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Microambiente Tumoral/genética , Proteína Axina/metabolismoRESUMO
OBJECTIVE: Thyroid cancer (TC) is one of the fastest-growing malignant tumors. This study sought to explore the mechanism of immune escape mediated by receptor tyrosine kinase (KIT) in TC. METHODS: The expression microarray of TC was acquired through the GEO database, and the difference analysis and Kyoto encyclopedia of genes and genomes pathway enrichment analysis were carried out. KIT levels in TC cell lines (K1/SW579/BCPAP) and human normal thyroid cells were detected using reverse transcription quantitative polymerase chain reaction and western blot analysis. TC cells were transfected with overexpressed (oe)-KIT and CD8+ T cells were cocultured with SW579 cells. Subsequently, cell proliferation, migration, and invasion abilities, CD8+ T cell proliferation, cytokine levels (interferon-γ [IFN-γ]/tumor necrosis factor-α [TNF-α]) were determined using colony formation assay, Transwell assays, flow cytometry, and enzyme-linked immunosorbent assay. The phosphorylation of MAPK pathway-related protein (ERK) was measured by western blot analysis. After transfection with oe-KIT, cells were treated with anisomycin (an activator of the MAPK pathway), and the protein levels of p-ERK/ERK and programmed death-ligand 1 (PD-L1) were detected. RESULTS: Differentially expressed genes (N = 2472) were obtained from the GEO database. KIT was reduced in TC samples and lower in tumor cells than those in normal cells. Overexpression of KIT inhibited immune escape of TC cells. Specifically, the proliferation, migration, and invasion abilities of TC cells were lowered, the proliferation level of CD8+ T cells was elevated, and IFN-γ and TNF-α levels were increased. KIT inhibited the activation of the MAPK pathway in TC cells and downregulated PD-L1. CONCLUSION: KIT suppressed immune escape of TC by blocking the activation of the MAPK pathway and downregulating PD-L1.
Assuntos
Antígeno B7-H1 , Neoplasias da Glândula Tireoide , Humanos , Proliferação de Células/genética , Proteínas Tirosina Quinases , Neoplasias da Glândula Tireoide/genética , Neoplasias da Glândula Tireoide/patologia , Fator de Necrose Tumoral alfaRESUMO
Toxic effects of abamectin on non-target aquatic organisms have been well documented due to its extensive use in both agricultural and aquacultural areas. However, knowledge of the abamectin induced cytotoxicity in crustacean hepatopancreas is still incomplete. In this study, we investigated the cytotoxic effects of abamectin on hepatopancreas cells of Chinese mitten crab, Eriocheir sinensis by an in vitro assay. The results showed that abamectin inhibited cell viability with elevated reactive oxygen species (ROS) and malondialdehyde (MDA) levels in a dose-dependent manner. Increased olive tail moment (OTM) values and 8-hydroxy-2'-deoxyguanosine (8-OHdG) contents indicate the DNA damage under abamectin exposure. The up-regulation of the typical apoptosis-related protein BCL2-associated X protein (Bax) and the down-regulation of B cell leukemia/lymphoma 2 (Bcl-2) demonstrate apoptosis in hepatopancreas cells. Meanwhile, the activities of both caspase-3 and caspase-9 were increased, indicating caspase-mediated apoptosis. In addition, qRT-PCR results showed the up-regulation of antioxidant genes superoxide dismutase (SOD) and catalase (CAT). The mRNA expression of Cap 'n' Collar isoform-C (CncC) and c-Jun NH2-terminal kinases (JNK) was also significantly increased, implying the involvement of the Nrf2/MAPK pathway in the antioxidative response. The alteration of innate immune-associated genes Toll-like receptor (TLR) and myeloid differentiation primary response gene 88 (Myd88) also indicates the influence of abamectin on immune status. In summary, the present study reveals the cytotoxicity of abamectin on hepatopancreas cells of E. sinensis and this in vitro cell culture model could be used for further assessment of pesticide toxicity.
RESUMO
In this study, an attempt was made to clarify the impact of substrates on the microbial fuel cell coupled with constructed wetland (CW-MFC) towards the treatment of nickel-containing wastewater. Herein, zeolite (ZEO), coal cinder (COA), ceramsite (CER), and granular activated carbon (GAC) were respectively introduced into lab-scaled CW-MFCs to systematically investigate the operational performances and microbial community response. GAC was deemed as the most effective substrate, and the corresponding device yielded favorable nickel removal efficiencies over 99% at different initial concentrations of nickel. GAC-CW-MFC likewise produced a maximum output voltage of 573 mV, power density of 8.95 mW/m2, and internal resistance of 177.9 Ω, respectively. The strong adsorptive capacity of nickel by GAC, accounting for 54.5% of total contaminant content, was mainly responsible for the favorable nickel removal performances of device GAC-CW-MFC. The high-valence Ni2+ was partially reduced to elemental Ni0 on the cathode, which provided evidence for the removal of heavy metals via the cathodic reduction of CW-MFC. The microbial community structure varied considerably as a result of substrates addition. For an introduction of GAC into the CW-MFC, a remarkably enriched population of genera Thermincola, norank_f__Geobacteraceae, Anaerovorax, Bacillus, etc. was noted. This study was dedicated to providing a theoretical guidance for an effective regulation of CW-MFC treatment on nickel-containing wastewater and accompanied by bioelectricity generation via the introduction of optimal substrate.
Assuntos
Fontes de Energia Bioelétrica , Níquel , Águas Residuárias , Áreas Alagadas , Eletrodos , EletricidadeRESUMO
In order to deeply analyze the application of CT images based on artificial intelligence algorithm in clinical treatment of AIDS patients with gastric cancer, and to provide reference for intervention of AIDS patients with gastric cancer, a total of 100 AIDS patients with gastric cancer were included as the research objects. The patients with CD4+ T lymphocyte count less than 200 cells/µL were in the control group (50 cases), whereas those higher than 200 cells/µL were in the experimental group (50 cases). All the patients underwent 64-slice spiral CT scanning. An improved algebraic reconstruction technology (ART) under L0 algorithmic approach (L0-ART) was proposed, and compared with the total variation (TV), filtered back projection (FBP), and weighted total variation (WTV) models. The standard deviation (STD) and average processing time of the L0-ART algorithm were significantly lower than those of the TV, FBP, and WTV algorithms (P < 0.05). The operation time of the experimental group was longer than that of the control group; the intraoperative blood loss, the diameter of the surgical wound, the time of first farting, the length of hospital stays, and the incidence of adverse reactions were all greatly lower than those of the control group (P < 0.05). Postoperatively, the total adipose tissue (TAT), visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in the abdominal area were higher in the experimental group than those in the control group (P < 0.05). In conclusion, the improved L0-ART algorithm proposed in this study had an excellent processing effect on CT images with a clinical promotion value. Patients with CD4+ T lymphocytes over 200 cells/µL had better surgical outcomes and prognosis than those with less than 200 cells/µL.
Assuntos
Síndrome da Imunodeficiência Adquirida , Neoplasias Gástricas , Algoritmos , Inteligência Artificial , Humanos , Interpretação de Imagem Radiográfica Assistida por Computador/métodos , Neoplasias Gástricas/complicações , Neoplasias Gástricas/diagnóstico por imagem , Neoplasias Gástricas/cirurgia , Tomografia Computadorizada Espiral , Tomografia Computadorizada por Raios X/métodosRESUMO
This study was carried out to explore the preoperative predictive value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in extramural vascular invasion (EMVI) in patients with rectal cancer. 124 patients with rectal cancer were randomly divided into two groups, with 62 groups in each group. One group used conventional magnetic resonance imaging (MRI) and was recorded as the control group. The other group used DCE-MRI and was recorded as the experimental group. The diagnostic value was evaluated by comparing the MRI quantitative parameters of EMVI positive and EMVI negative patients, as well as the area under the curve (AUC) of the receiver operating characteristic curve (ROC), diagnostic sensitivity, and specificity of the two groups. The results showed that the Ktrans and Ve values of EMVI positive patients in the experimental group and the control group were 1.08 ± 0.97 and 1.03 ± 0.93, and 0.68 ± 0.29 and 0.65 ± 0.31, respectively, which were significantly higher than those in EMVI negative patients (P < 0.05). The AUC of EMVI diagnosis in the experimental group and the control group were 0.732 and 0.534 (P < 0.05), the sensitivity was 0.913 and 0.765 (P < 0.05), and the specificity was 0.798 and 0.756 (P > 0.05), respectively. In conclusion, DCE-MRI has a higher diagnostic value than conventional MRI in predicting EMVI in patients with rectal cancer, which was worthy of further clinical promotion.
Assuntos
Neoplasias Retais , Humanos , Imageamento por Ressonância Magnética/métodos , Invasividade Neoplásica/diagnóstico por imagem , Invasividade Neoplásica/patologia , Curva ROC , Neoplasias Retais/diagnóstico por imagem , Neoplasias Retais/patologia , Estudos RetrospectivosRESUMO
The infusion of coronavirus disease 2019 (COVID-19) patients with mesenchymal stem cells (MSCs) potentially improves clinical symptoms, but the underlying mechanism remains unclear. We conducted a randomized, single-blind, placebo-controlled (29 patients/group) phase II clinical trial to validate previous findings and explore the potential mechanisms. Patients treated with umbilical cord-derived MSCs exhibited a shorter hospital stay (P = 0.0198) and less time required for symptoms remission (P = 0.0194) than those who received placebo. Based on chest images, both severe and critical patients treated with MSCs showed improvement by day 7 (P = 0.0099) and day 21 (P = 0.0084). MSC-treated patients had fewer adverse events. MSC infusion reduced the levels of C-reactive protein, proinflammatory cytokines, and neutrophil extracellular traps (NETs) and promoted the maintenance of SARS-CoV-2-specific antibodies. To explore how MSCs modulate the immune system, we employed single-cell RNA sequencing analysis on peripheral blood. Our analysis identified a novel subpopulation of VNN2+ hematopoietic stem/progenitor-like (HSPC-like) cells expressing CSF3R and PTPRE that were mobilized following MSC infusion. Genes encoding chemotaxis factors - CX3CR1 and L-selectin - were upregulated in various immune cells. MSC treatment also regulated B cell subsets and increased the expression of costimulatory CD28 in T cells in vivo and in vitro. In addition, an in vivo mouse study confirmed that MSCs suppressed NET release and reduced venous thrombosis by upregulating kindlin-3 signaling. Together, our results underscore the role of MSCs in improving COVID-19 patient outcomes via maintenance of immune homeostasis.
Assuntos
COVID-19/terapia , Imunomodulação , Transplante de Células-Tronco Mesenquimais , Idoso , Animais , Anticorpos Antivirais/sangue , Subpopulações de Linfócitos B/citologia , Subpopulações de Linfócitos B/imunologia , Subpopulações de Linfócitos B/metabolismo , Proteína C-Reativa/análise , COVID-19/imunologia , COVID-19/virologia , Citocinas/genética , Citocinas/metabolismo , Proteínas do Citoesqueleto/metabolismo , Modelos Animais de Doenças , Armadilhas Extracelulares/metabolismo , Feminino , Humanos , Leucócitos Mononucleares/citologia , Leucócitos Mononucleares/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , SARS-CoV-2/isolamento & purificação , Linfócitos T/citologia , Linfócitos T/imunologia , Linfócitos T/metabolismo , Trombose Venosa/metabolismo , Trombose Venosa/patologiaRESUMO
Objective To explore whether oncolytic adenovirus expressing CCL19 can inhibit the growth of gastric cancer cells and activate anti-tumor immune response. Methods Mouse CCL19 gene was inserted into the E3 region of oncolytic adenovirus Ad5 to obtain engineered oncolytic adenovirus Ad5-CCL19. The expression of CCL19 in Ad5-CCL19-infected mouse MFC cells was detected by Western blotting. The effects of Ad5-CCL19 on the proliferation of MFC cells, MGC803 cells and BGC823 cells were tested by MTT assay. The anti-tumor activity of Ad5-CCL19 in vivo was examined by MFC cell subcutaneous transplantation tumor model. Immunofluorescence histochemical staining was used to detect CD4 and CD8 expression in tumor tissue. The secretion levels of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) in tumor infiltrating T cells were detected by flow cytometry. Results Ad5-CCL19 could effectively infect MFC cells to secrete CCL19. Also, Ad5-CCL19 could induce significant dose-dependent cytotoxicity against target cells in vitro. The experiment in vivo showed that Ad5-CCL19 had stronger inhibitory effects on MFC cell tumor than Ad5 in the mice, and it could effectively enhance the infiltration of CD4+ T cells and CD8+ T cells and increase the secretion of IFN-γ and TNF-α in tumor tissues. Conclusion Ad5-CCL19 can significantly infect MFC gastric cancer cells to inhibit their growth and improve the anti-tumor immune activity of the tumor site.
Assuntos
Terapia Viral Oncolítica , Neoplasias Gástricas , Adenoviridae/genética , Animais , Linfócitos T CD8-Positivos , Linhagem Celular Tumoral , Quimiocina CCL19 , Imunidade , Camundongos , Neoplasias Gástricas/genética , Neoplasias Gástricas/terapiaRESUMO
The mechanism of natural-hazard-triggered technological (Na-tech) cascading disasters is complex, and the extent to which their damage is aggravated by various secondary events is difficult to quantify. This study selected a large oil depot and constructed a full-scale three-dimensional scene model based on the surrounding geographical environment. The discrete element method (DEM), finite element method (FEM) and finite volume method (FVM) were employed to conduct numerical simulations of the process and consequences of the following Na-tech disasters: heavy-rainfall-induced landslide â blocks impacting an oil transportation pipeline and breaking it â oil leaking, spreading and resulting in a vapor cloud explosion. According to the results, the maximum impact of the 1 m3 of sliding mass formed in the landslide on the pipeline was over 7 MN (meganewton), and the pipeline fractured completely when it was loaded with a contact force of only 1.44 MN. The numerical simulation methods revealed the mechanism of Na-tech cascading disasters in a large oil depot and quantified the consequences of each event in the cascading disasters.
Assuntos
Desastres , Deslizamentos de Terra , Meio Ambiente , TecnologiaRESUMO
BACKGROUND: COVID-19 (coronavirus disease 2019) has become a global public health emergency since patients were first detected in Wuhan, China, in December 2019. Currently, there are no satisfying antiviral medications and vaccines available. CASE PRESENTATION: We reported the treatment process and clinical outcome of a 48-year-old man critically ill COVID-19 patient who received transfusion of allogenic human umbilical cord mesenchymal stem cells (UC-MSCs). CONCLUSIONS: We proposed that UC-MSC transfusion might be a new option for critically ill COVID-19. Although only one case we were shown, more similar clinical cases are inquired for further evidence providing the potential effectiveness of UC-MSC treatment.
RESUMO
Quorum sensing is a population density-dependent gene expression regulation mechanism in bacteria. The substrate specificity of RhlI, an enzyme in the RhlI-RhlR quorum sensing system of Pseudomonas aeruginosa, was explored by directed evolution to gain insight into the molecular mechanisms of quorum sensing. RhlI catalyzes S-adenosyl methionine and butanoyl or hexanoyl acyl carrier protein to form N-butanoyl homoserine lactone (BHL) and or N-hexanoyl homoserine lactone (HHL), respectively, none of which contain 3-oxo groups. We developed high-throughput genetic screening and selection methods to identify RhlI mutants via four rounds of directed evolution and identified RhlI-4M1 as the mutant that generated new catalytic activity and synthesized 3-oxo-hexanoyl homoserine lactone (OHHL) containing the 3-oxo group in Escherichia coli. Additionally, the synthesizing activities of BHL and HHL were improved by 3.98- and 3.01-fold, respectively. RhlI-4M1 contains five amino acid substitutions (A15D, K31R, T92S, Y129N, and L184Q) and one stop codon (Q193*) mutations. The deletion of nine amino acids in the C-terminus was crucial for OHHL production by RhlI mutants. This work demonstrates that the genetic screen/selection should be useful in the development of applications involving the manipulation of bacterial quorum sensing. The new catalytic activity of these RhlI mutants will prove beneficial in elucidating the mechanistic understanding of bacterial quorum sensing and similarly, may prove beneficial in the development of new drugs including antimicrobial compounds.
Assuntos
Evolução Molecular Direcionada/métodos , Ligases/genética , Pseudomonas aeruginosa/genética , Percepção de Quorum , Fatores de Transcrição/genética , 4-Butirolactona/análogos & derivados , 4-Butirolactona/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Catálise , Escherichia coli/genética , Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica , Ensaios de Triagem em Larga Escala , Ligases/metabolismo , Mutação , Pseudomonas aeruginosa/enzimologia , S-Adenosilmetionina/metabolismo , Especificidade por Substrato , Fatores de Transcrição/metabolismoRESUMO
PURPOSE: This study aimed to investigate the efficacy and safety of transanal endoscopic microsurgery for radical resection of sigmoid cancer. METHODS: 91 patients with sigmoid cancer who underwent sigmoid cancer resection were divided to the Control Group (43 patients who underwent conventional laparoscopic surgery and pathological specimens were taken through the abdomen) and the Study Group (48 patients who were subjected to transanal endoscopic microsurgery and pathological specimens were taken through the anus). Comparisons were made about the operation time and the amount of surgical bleeding of the two groups, as well as the postoperative exhaust time and postoperative visual analogue score (VAS) of the two groups. Also, factors like the complications, postoperative hospitalization time, additional analgesic treatment, and treatment efficacy of patients within the first month after the surgery were compared between the two groups. Finally, a 3-year follow up for patients was performed to record the 3-year recurrence rates. RESULTS: The operation time and the amount of surgical bleeding of the Study Group were significantly lower than those of the Control Group (p<0.05); the 3-year recurrence rate of the Study Group was lower than that of the Control Group. On the contrary, the 3-year survival rate of the Study Group was significantly higher than that of the Control Group (p<0.05). CONCLUSION: The application of transanal endoscopic microsurgery for radical resection of sigmoid cancer is worthy of clinical promotion despite its high technical requirements for the surgeon and certain degree of promotion difficulty, since it boasts high effective rate, low rate of complications, and the contribution for decreased recurrence rate and improved survival. Key words: anal endoscopic microsurgery, sigmoid cancer, effectiveness, safety analysis.
Assuntos
Adenocarcinoma/cirurgia , Neoplasias Retais/cirurgia , Neoplasias do Colo Sigmoide/cirurgia , Microcirurgia Endoscópica Transanal , Adenocarcinoma/patologia , Canal Anal/patologia , Canal Anal/cirurgia , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Intervalo Livre de Progressão , Neoplasias Retais/patologia , Neoplasias do Colo Sigmoide/patologiaRESUMO
The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4). Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1). The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2. Except for C4, cordycepin was not detected in C1-C3 or S1. The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3. Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3. Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2. These results can help us better understand products of Cordyceps strains.
Assuntos
Antineoplásicos/análise , Cordyceps/química , Polissacarídeos/análise , Aminoácidos/análise , China , Ácidos Graxos/análise , Fermentação , Carpóforos/química , Minerais/análise , Monossacarídeos/análise , Micélio/química , Nucleosídeos/análise , Triterpenos/análiseRESUMO
Baseline blood concentrations of metals are important references for monitoring metal exposure in environmental and occupational settings. The purpose of this study was to determine the blood levels of manganese (Mn), copper (Cu), zinc (Zn), lead (Pb), and cadmium (Cd) among the residents (aged 12-60 years old) living in the suburb southwest of Beijing in China and to compare the outcomes with reported values in various developed countries. Blood samples were collected from 648 subjects from March 2009 to February 2010. Metal concentrations in the whole blood were determined by ICP-MS. The geometric means of blood levels of Mn, Cu, Zn, Pb and Cd were 11.4, 802.4, 4665, 42.6, and 0.68 µg/L, respectively. Male subjects had higher blood Pb than the females, while the females had higher blood Mn and Cu than the males. There was no gender difference for blood Cd and Zn. Smokers had higher blood Cu, Zn, and Cd than nonsmokers. There were significant age-related differences in blood levels of all metals studied; subjects in the 17-30 age group had higher blood levels of Mn, Pb, Cu, and Zn, while those in the 46-60 age group had higher Cd than the other age groups. A remarkably lower blood level of Cu and Zn in this population as compared with residents of other developed countries was noticed. Based on the current study, the normal reference ranges for the blood Mn were estimated to be 5.80-25.2 µg/L; for blood Cu, 541-1475 µg/L; for blood Zn, 2349-9492 µg/L; for blood Pb, <100 µg/L; and for blood Cd, <5.30 µg/L in the general population living in Beijing suburbs.