Agonistic and reproductive behaviors in males of red hybrid tilapia, Oreochromis niloticus (Linnaeus, 1758) x O. mossambicus (Peters, 1852) (Osteichthyes: Cichlidae)

The red hybrid tilapia, Oreochromis niloticus (Linnaeus, 1758) x O. mossambicus (Peters, 1852) is a fertile hybrid used in the semi-intensive level of fish culture in the Northeast of Brazil. It is a territorial cichlid and is highly aggressive towards conspecifics during the breeding season. The purpose of this study was to investigate and describe the aggressive behaviour displayed by the males of this hybrid in non-reproductive and reproductive contexts. Behavioural observations revealed that aggression displayed by the reproductive males of red hybrid tilapia included threatening, undulation, parallel, lateral and frontal attacks, chasing, escape and submission. Possession of a territory influenced male aggressiveness, which was more intense in their own territory than that observed in a neutral situation. The males built nests, irrespective of female presence. All the behavioural patterns were in accordance with those previously described for one parental species, the Nile tilapia, O. niloticus.

O híbrido vermelho de tilápia Oreochromis niloticus (Linnaeus, 1758) x O. mossambicus (Peters, 1852) é um híbrido fértil utilizado na piscicultura numa escala semi-intensiva no Nordeste brasileiro. É um ciclídeo territorial e altamente agressivo frente a coespecíficos na época reprodutiva. O objetivo deste estudo foi investigar e descrever os comportamentos agressivos exibidos pelos machos deste híbrido em contextos não-reprodutivos e reprodutivos. As observações comportamentais mostraram que as agressões exibidas pelos machos de híbrido vermelho de tilápia incluíram ameaça, ondulação, confronto paralelo, lateral e frontal, perseguição, fuga e submissão. A posse do território influenciou a agressividade dos machos, cuja intensidade foi maior no próprio território em relação àquela observada em situação neutra. Os ninhos foram escavados pelos machos independente da presença das fêmeas. Todos os padrões comportamentais descritos estavam de acordo com os padrões previamente descritos para uma das espécies parentais, a Tilápia do Nilo, O. niloticus.

Mating system of the amazonian cichlid angel fish, Pterophyllum scalare

The species, Pterophyllum scalare distinguishes itself by its breeding behavior, involving competition for territory, sexual partners, courtship and parental care. The purpose of this study was to identify the mating system adopted by this species of fish. Twenty males and twenty females were observed under semi-natural and experimental conditions to test the hypothesis of serial monogamy. Under semi-natural conditions, after the third breeding cycle, the couples changed mates. Under experimental conditions, the couples changed partners after the first breeding cycle. Under experimental conditions, mate recognition was investigated through the preference of the females, indicated by the time they spent with the males. The females were available or not for courtship from new males, depending on their aggressiveness or submission. The larger and more aggressive males obtained new mating opportunities while the submissive males were rejected by the females. The mated fish were aggressive towards intruders in the presence of the mate, protecting their pair bond. In the interval between breeding cycles, the couples did not display aggression towards intruders, confirming the hypothesis of serial monogamy. Best mate selection by the females and the opportunity of new matings for both sexes influenced the reproductive success of this species.

A espécie Pterophyllum scalare se destaca pela complexidade de seu comportamento reprodutivo, envolvendo competição por território e parceiros sexuais, corte, acasalamento e cuidado parental. Este trabalho teve como objetivo identificar o tipo de sistema de acasalamento adotado pelos peixes desta espécie. Vinte machos e vinte fêmeas foram observados nas situações seminatural e experimental para testar a hipótese de monogamia serial. Em situação seminatural, após o terceiro ciclo reprodutivo, os casais mudaram de parceiros, enquanto que, em situação experimental, os casais mudaram de parceiros a partir do primeiro ciclo reprodutivo. Em situação experimental foi investigado o reconhecimento do parceiro, pela permanência das fêmeas que se mostraram disponíveis ou não à corte de novos machos, em função da agressividade e submissão deles. Machos maiores e mais agressivos obtiveram novas oportunidades de acasalamento e machos submissos foram rejeitados pelas fêmeas. Os peixes acasalados foram agressivos a intrusos na presença do parceiro, protegendo a ligação do par. No intervalo entre os ciclos reprodutivos, os pares não mostraram agressão a intrusos, confirmando a hipótese de monogamia serial. A escolha do melhor parceiro pelas fêmeas e a oportunidade de novos acasalamentos para ambos os sexos influenciaram o sucesso reprodutivo desta espécie.

Contested dominance modifies the anovulatory consequences of social subordination in female marmosets.

Dominance status among female marmosets is reflected in agonistic behavior and ovarian function. Socially dominant females receive submissive behavior from subordinates, while exhibiting normal ovulatory function. Subordinate females, however, receive agonistic behavior from dominants, while exhibiting reduced or absent ovulatory function. Such disparity in female fertility is not absolute, and groups with two breeding females have been described. The data reported here were obtained from 8 female-female pairs of captive female marmosets, each housed with a single unrelated male. Pairs were classified into two groups: "uncontested" dominance (UD) and "contested" dominance (CD), with 4 pairs each. Dominant females in UD pairs showed significantly higher frequencies (4.1) of agonism (piloerection, attack and chasing) than their subordinates (0.36), and agonistic behaviors were overall more frequently displayed by CD than by UD pairs. Subordinates in CD pairs exhibited more agonistic behavior (2.9) than subordinates in UD pairs (0.36), which displayed significantly more submissive (6.97) behaviors than their dominants (0.35). The data suggest that there is more than one kind of dominance relationship between female common marmosets. Assessment of progesterone levels showed that while subordinates in UD pairs appeared to be anovulatory, the degree of ovulatory disruption in subordinates of CD pairs was more varied and less complete. We suggest that such variation in female-female social dominance relationships and the associated variation in the degree and reliability of fertility suppression may explain variations of the reproductive condition of free-living groups of common marmosets.

Contested dominance modifies the anovulatory consequences of social subordination in female marmosets

Dominance status among female marmosets is reflected in agonistic behavior and ovarian function. Socially dominant females receive submissive behavior from subordinates, while exhibiting normal ovulatory function. Subordinate females, however, receive agonistic behavior from dominants, while exhibiting reduced or absent ovulatory function. Such disparity in female fertility is not absolute, and groups with two breeding females have been described. The data reported here were obtained from 8 female-female pairs of captive female marmosets, each housed with a single unrelated male. Pairs were classified into two groups: "uncontested" dominance (UD) and "contested" dominance (CD), with 4 pairs each. Dominant females in UD pairs showed significantly higher frequencies (4.1) of agonism (piloerection, attack and chasing) than their subordinates (0.36), and agonistic behaviors were overall more frequently displayed by CD than by UD pairs. Subordinates in CD pairs exhibited more agonistic behavior (2.9) than subordinates in UD pairs (0.36), which displayed significantly more submissive (6.97) behaviors than their dominants (0.35). The data suggest that there is more than one kind of dominance relationship between female common marmosets. Assessment of progesterone levels showed that while subordinates in UD pairs appeared to be anovulatory, the degree of ovulatory disruption in subordinates of CD pairs was more varied and less complete. We suggest that such variation in female-female social dominance relationships and the associated variation in the degree and reliability of fertility suppression may explain variations of the reproductive condition of free-living groups of common marmosets.

Behavior and progesterone levels in Callithrix jacchus females.

In callitrichids, and especially in the Callithrix and Saguinus genera, reproductive suppression maintains the breeding exclusivity of dominant females. It has been suggested that dominance may be inferred from agonistic behavior of females. The purpose of this study was to assess the adequacy of behaviors as indicators of hormonal status in Callithrix jacchus females. Two pairs of twin females were observed for affiliative, agonistic and sexual behaviors for 20 weeks, in four stages of increasingly potential competition for one male. During the same period, blood samples were taken regularly for progesterone determination by ELISA. The two pairs differed markedly as to behaviors and hormonal status: pair 1 showed a well established behavioral dominance and only one ovulating female from the beginning of the observation period; pair 2 showed competition for dominance until the last stage of observation, when one of the females started ovulating. Correlations between behaviors and progesterone levels showed differences between subordinate and dominant females mostly in pair 2. Although limited in number of animals studied, these results suggest that there is a relationship between behavior and hormones in female marmosets, but this relationship may be useful in the identification of the physiological status of females only in groups where the dominance is not well established.

Behavior and progesterone levels in Callithrix jacchus females

In callitrichids, and especially in the Callithrix and Saguinus genera, reproductive suppression maintains the breeding exclusivity of dominant females. It has been suggested that dominance may be inferred from agonistic behavior of females. The purpose of this study was to assess the adequacy of behaviors as indicators of hormonal status in Callithrix jacchus females. Two paires of twin females were observed for affiliative, agonistic and sexual behaviors for 20 weeks, in four stages of increasingly potential competition for one male. During the same period, blood samples were taken regularly for progesterone determination by ELISA. The two pairs differed markedly as to behaviors and hormonal status: pair 1 showed a well established behavioral dominance and only one ovulating female from the beginning of the observation period; pair 2 showed competition for dominance until the last stage of observation, when one of the females started ovulating. Correlations between behaviors and progesterone levels showed differences between subordinate and dominant females mostly in pair 2. Although limited in number of animals studied, these results suggest that there is a relationship between behavior and hormones in female marmosets, but this relationship may be useful in the identification of the physiological status of females only in groups where the dominance is not well estblished