Highly efficient photocathodic cascade material for constructing sensitive photoelectrochemical biosensor.

Photocathodic biosensor possesses excellent anti-interference capability in bioanalysis, which however suffers from high electron-hole recombination rate with low photocurrent. Herein, a high-performance inorganic organic P3HT@C60@ZnO nanosphere with cascade energy band arrangement was synthesized as photoactive signal probe, which inherited the advantages of inorganic strong optical absorptivity and organic high mobility for photo-generated holes. Specifically, the well-matched band gap endowed not only the improved life for light generated carrier and promoted separation of electron-hole pairs, but also the expansion of charge-depletion layer, significantly improving the photoelectric conversion efficiency for acquiring an extremely high photocathodic signal that increased by 30 times compared with individual materials. Accordingly, by integrating with the efficient amplification of DNA nanonet derived from clamped hybrid chain reaction (C-HCR), a sensitive P3HT@C60@ZnO nanosphere based photocathodic biosensor was proposed for accurate detection of p53. The experimental results showed that the biosensor had a wide detection range from 0.1 fM to 10 nM and a low detection limit of 0.37 fM toward p53, offering a new avenue to construct sensitive PEC platform with superior anti-interference ability and hold a prospective application in early disease diagnosis and biological analysis.

[Analysis of serum reproductive hormone levels in men with hyperlipemia in the urban area of Shijiazhuang].

OBJECTIVE: To investigate the aging-related changes of reproductive hormones in hyperlipemia men in the urban area of Shijiazhuang and the relationship between the levels of reproductive hormones and that of blood lipid. METHODS: We enrolled 297 males aged 25－65 years in this study, determined the levels of serum total testosterone (TT), luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol (E2), prolactin (PRL), triglyeride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and fasting blood glucose (FBG), and compared the parameters among different age groups. RESULTS: The level of serum TT was decreased while that of FSH increased significantly in the 36－45 years group and that of LH elevated only in the 56－65 years old hyperlipemia males(P<0.05). No statistically significant changes were observed in PRL and E2 among different age groups. Spearman correlation analysis manifested that age was correlated negatively with TT, FSH, and LH (r=－0.266, 0.514, and 0.350, P<0.05), TT weakly negatively with TC and TG (r=－0.215 and －0.286, P<0.05) but weakly positively with HDL-C (r=0.281, P<0.01), and FSH and LH weakly positively with TG (r=0.282 and 0.343, P<0.05). Regression analysis showed age to be an independent influencing factor of TT, FSH and LH, while HDL-C to be that of TT. CONCLUSIONS: The levels of serum TT and FSH gradually change with aging and low TT and high FSH and LH are closely related to hyperlipemia in adult men.