Roles of very long-chain fatty acids in compound leaf patterning in Medicago truncatula.

Plant cuticles are composed of hydrophobic cuticular waxes and cutin. Very long-chain fatty acids (VLCFAs) are components of epidermal waxes and the plasma membrane and are involved in organ morphogenesis. By screening a barrelclover (Medicago truncatula) mutant population tagged by the transposable element of tobacco (Nicotiana tabacum) cell type1 (Tnt1), we identified two types of mutants with unopened flower phenotypes, named unopened flower1 (uof1) and uof2. Both UOF1 and UOF2 encode enzymes that are involved in the biosynthesis of VLCFAs and cuticular wax. Comparative analysis of the mutants indicated that the mutation in UOF1, but not UOF2, leads to the increased number of leaflets in M. truncatula. UOF1 was specifically expressed in the outermost cell layer (L1) of the shoot apical meristem (SAM) and leaf primordia. The uof1 mutants displayed defects in VLCFA-mediated plasma membrane integrity, resulting in the disordered localization of the PIN-FORMED1 (PIN1) ortholog SMOOTH LEAF MARGIN1 (SLM1) in M. truncatula. Our work demonstrates that the UOF1-mediated biosynthesis of VLCFAs in L1 is critical for compound leaf patterning, which is associated with the polarization of the auxin efflux carrier in M. truncatula.

Molecular mechanisms of cell death in bronchopulmonary dysplasia.

Bronchopulmonary dysplasia (BPD) in neonates is the most common pulmonary disease that causes neonatal mortality, has complex pathogenesis, and lacks effective treatment. It is associated with chronic obstructive pulmonary disease, pulmonary hypertension, and right ventricular hypertrophy. The occurrence and development of BPD involve various factors, of which premature birth is the most crucial reason for BPD. Under the premise of abnormal lung structure and functional product, newborns are susceptible to damage to oxides, free radicals, hypoxia, infections and so on. The most influential is oxidative stress, which induces cell death in different ways when the oxidative stress balance in the body is disrupted. Increasing evidence has shown that programmed cell death (PCD), including apoptosis, necrosis, autophagy, and ferroptosis, plays a significant role in the molecular and biological mechanisms of BPD and the further development of the disease. Understanding the mode of PCD and its signaling pathways can provide new therapeutic approaches and targets for the clinical treatment of BPD. This review elucidates the mechanism of BPD, focusing on the multiple types of PCD in BPD and their molecular mechanisms, which are mainly based on experimental results obtained in rodents.

Effects of Antioxidants in Human Milk on Bronchopulmonary Dysplasia Prevention and Treatment: A Review.

Bronchopulmonary dysplasia (BPD) is a severe chronic lung illness that affects neonates, particularly premature infants. It has far-reaching consequences for infant health and their families due to intractable short- and long-term repercussions. Premature infant survival and long-term quality of life are severely harmed by BPD, which is characterized by alveolarization arrest and hypoplasia of pulmonary microvascular cells. BPD can be caused by various factors, with oxidative stress (OS) being the most common. Premature infants frequently require breathing support, which results in a hyperoxic environment in the developing lung and obstructs lung growth. OS can damage the lungs of infants by inducing cell death, inhibiting alveolarization, inducing inflammation, and impairing pulmonary angiogenesis. Therefore, antioxidant therapy for BPD relieves OS and lung injury in preterm newborns. Many antioxidants have been found in human milk, including superoxide dismutase, glutathione peroxidase, glutathione, vitamins, melatonin, short-chain fatty acids, and phytochemicals. Human milk oligosaccharides, milk fat globule membrane, and lactoferrin, all unique to human milk, also have antioxidant properties. Hence, human milk may help prevent OS injury and improve BPD prognosis in premature infants. In this review, we explored the role of OS in the pathophysiology of BPD and related signaling pathways. Furthermore, we examined antioxidants in human milk and how they could play a role in BPD to understand whether human milk could prevent and treat BPD.

Two R2R3-MYB genes cooperatively control trichome development and cuticular wax biosynthesis in Prunus persica.

The fruit surface has an enormous impact on the external appearance and postharvest shelf-life of fruit. Here, we report two functionally redundant genes, PpMYB25 and PpMYB26, involved in regulation of fruit skin texture in peach. PpMYB25 can activate transcription of PpMYB26 and they both induce trichome development and cuticular wax accumulation, resulting in peach fruit with a fuzzy and dull appearance. By contrast, nonfunctional mutation of PpMYB25 caused by an insertional retrotransposon in the last exon in nectarine fails to activate transcription of PpMYB26, resulting in nectarine fruit with a smooth and shiny appearance due to loss of trichome initiation and decreased cuticular wax accumulation. Secondary cell wall biosynthesis in peach fruit pubescence is controlled by a transcriptional regulatory network, including the master regulator PpNAC43 and its downstream MYB transcription factors such as PpMYB42, PpMYB46 and PpMYB83. Our results show that PpMYB25 and PpMYB26 coordinately regulate fruit pubescence and cuticular wax accumulation and their simultaneous perturbation results in the origin of nectarine, which is botanically classified as a subspecies of peach.

Synthesis of ethylene diamine-based ferrocene terminated dendrimers and their application as burning rate catalysts.

Ferrocene-based derivatives are widely used as ferrocene-based burning rate catalysts (BRCs) for ammonium perchlorate (AP)-based propellant. However, in long storage, small ferrocene-based derivatives migrate to the surface of the propellant, which results in changes in the designed burning parameters and finally causes unstable combustion. To retard the migration of ferrocene-based BRCs in the propellant and to increase the combustion of the solid propellant, zero to third generation ethylene diamine-based ferrocene terminated dendrimers (0G, 1G, 2G and 3G) were synthesized. The synthesis of these dendrimers was confirmed by 1H NMR and FT-IR spectroscopy. The electrochemical behavior of 0G, 1G, 2G and 3G was investigated by cyclic voltammetry (CV) and the burning rate catalytic activity of 0G, 1G, 2G and 3G on thermal disintegration of AP was examined by thermogravimetry (TG) and differential thermogravimetry (DTG) techniques. Anti-migration studies show that 1G, 2G and 3G exhibit improved anti-migration behavior in the AP-based propellant.

High efficiency degradation crude oil by a novel mutant irradiated from Dietzia strain by 12C6+ heavy ion using response surface methodology.

Crude oil is an extremely complex mixture of hydrocarbons; also contaminate environmental, leading to carcinogenic, teratogenic and mutagenic. Petroleum hydrocarbons degradation Dietzia strain DMYR9 was isolated from oilfield. Response surface methodology was applied for statistical designing of process parameters for dry weight of biomass production in the process of degradation. The optimization process parameters were successfully employed for degradation crude oil and confirmed through confirmatory experiments. On 28th day, analysis was done by GC-MS, These data show that the crude oil samples of n-Hexadecane, Octadecane, n-Nonadecanec, n-Pentacosane, n-Hexacosane, n-Heneicosane, n-Docosane, n-Tetracosane, n-Octacosane and Tetraethyl removal efficiency could reach up to 0%. RSM optimization and use of effective (12)C(6+)-ion irradiation methods can considerably enhance ability to degradation of microbial. Hence, bioresource Dietzia strain DMYR9, high ability to degradation, can be further used for subsequent repair hydrocarbons polluted of environment.