Calcium oxide nanoparticles mitigate lead stress in Abelmoschus esculentus though improving the key antioxidative enzymes, nutritional content and modulation of stress markers.

Lead (Pb) is thought to be one of most injurious metals on the earth. Lead stress in plants enhances synthesis of highly toxic reactive oxygen species (ROS). During present research, impact of calcium-oxide nanoparticles (CaO-NPs) was observed on antioxidative defense mechanism in Abelmoschus esculentus plants prone to Pb stress. A CRD experiment was employed with 5 replicates having four treatments (T0 = Control, T1 = Pb stress (200 ppm), T2 = CaO-NPs and T3 = Pb + CaO-NPs). Pb-stressed seedlings exhibited decreased root growth, shoot growth, chlorophyll concentration and biomass accumulation. Moreover, higher synthesis of hydrogen-peroxide (H2O2), malondialdehyde (MDA) and electrolyte leakage (EL) resulting in cellular injuries were noted in plants growing in Pb spiked conditions. Similarly, stressed plants showed higher accumulation of total soluble sugar and proline content besides elevated activity of antioxidative enzymes counting catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX). On the contrary side, CaO-NPs alleviated the Pb induced phytotoxicity through improving activity of antioxidative enzymes. The elevated activity of antioxidant enzymes reduced biosynthesis of H2O2 and MDA which was revealed through the increased growth parameters. In addition, CaO-NPs persuaded enhancement in plant defence machinery by decreased chlorophyll deprivation and augmented the uptake of plant nutrients including K and Ca content. Hence, CaO-NPs can be potent regulators of the antioxidative enzymes and stress markers to ameliorate abiotic stresses.

Potassium silicate and zinc oxide nanoparticles modulate antioxidant system, membranous H+-ATPase and nitric oxide content in faba bean (Vicia faba) seedlings exposed to arsenic toxicity.

Current research focused on the potential role of zinc oxide nanoparticles (ZnONPs) and potassium (K+ ) in mitigation of arsenic (As) toxicity in Vicia faba L. seedlings. Faba bean seedlings were grown for 30days in potted soil. As stress curtailed root and shoot length, chlorophyll (Chl) content and net photosynthetic rate in V. faba seedlings. However, ZnONPs and K+ curtailed As stress in faba bean seedling through enhanced activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) enzyme. Furthermore, ZnONPs and K+ significantly enhanced cysteine (Cys) content and serine acetyletransferase (SAT) activity in faba bean seedling exposed to As-toxificated soil. Application of ZnONPs and K+ curtailed superoxide ionic content and hydrogen peroxide (H2 O2 ) accumulation in V. faba seedlings exposed to As-polluted soil. Nitric oxide (NO) content also increased in faba bean seedlings treated with ZnONPs and K+ in normal and As-polluted soil. As stress alleviation was credited to reduce As uptake in faba bean seedlings treated with synergistic application of ZnONPs and K+ . It is proposed that K+ interaction with nanoparticles can be exploited at molecular level to understand the mechanisms involved in abiotic stress tolerance.

Iron oxide nanoparticles and selenium supplementation improve growth and photosynthesis by modulating antioxidant system and gene expression of chlorophyll synthase (CHLG) and protochlorophyllide oxidoreductase (POR) in arsenic-stressed Cucumis melo.

Current research reveals the positive role of iron oxide nanoparticles (IONPs) and selenium (Se) in extenuation of arsenic (As) induced toxicity in Cucumis melo. C. melo plants grown in As spiked soil (20 mg kg-1 As) showed reduced growth, chlorophyll (Chl) content, photosynthetic rate, stomatal conductivity and transpiration. On the other hand, the alone applications of IONPs or Se improved growth and physiochemical parameters of C. melo plants. Additionally, exogenous application IONPs and Se synergistically improved the activity of antioxidative enzymes and glyoxalase system in C. melo plants. In addition, the collective treatment of IONPs and Se reduced As uptake, enhanced rate of photosynthesis and increased gas exchange attributes of C. melo plants under As stress. Interactive effect of IONPs and Se regulated reduced glutathione (GSH), oxidized glutathione (GSSG) and ascorbate (AsA) content in C. melo plants exposed to As-contaminated Soil. IONPs and Se treatment also regulated expression of respiratory burst oxidase homologue D (RBOHD) gene, chlorophyll synthase (CHLG) and protochlorophyllide oxidoreductase (POR). Therefore, the combined treatment of IONPs and Se may enhance the growth of crop plants by alleviating As stress.

Iron Oxide and Silicon Nanoparticles Modulate Mineral Nutrient Homeostasis and Metabolism in Cadmium-Stressed Phaseolus vulgaris.

The application of nanoparticles (NPs) has been proved as an efficient and promising technique for mitigating a wide range of stressors in plants. The present study elucidates the synergistic effect of iron oxide nanoparticles (IONPs) and silicon nanoparticles (SiNPs) in the attenuation of Cd toxicity in Phaseolus vulgaris. Seeds of P. vulgaris were treated with IONPs (10 mg/L) and SiNPs (20 mg/L). Seedlings of uniform size were transplanted to pots for 40 days. The results demonstrated that nanoparticles (NPs) enhanced growth, net photosynthetic rate, and gas exchange attributes in P. vulgaris plants grown in Cd-contaminated soil. Synergistic application of IONPs and SiNPs raised not only K+ content, but also biosynthesis of polyamines (PAs), which alleviated Cd stress in P. vulgaris seedlings. Additionally, NPs decreased malondialdehyde (MDA) content and electrolyte leakage (EL) in P. vulgaris plants exposed to Cd stress. These findings suggest that stress alleviation was mainly attributed to the enhanced accumulation of K+ content, improved antioxidant defense system, and higher spermidine (Spd) and putrescine (Put) levels. It is suggested that various forms of NPs can be applied synergistically to minimize heavy metal stress, thus increasing crop production under stressed conditions.

Calcium Nanoparticles Impregnated With Benzenedicarboxylic Acid: A New Approach to Alleviate Combined Stress of DDT and Cadmium in Brassica alboglabra by Modulating Bioacummulation, Antioxidative Machinery and Osmoregulators.

At present, the alleviation of stress caused by climate change and environmental contaminants is a crucial issue. Dichlorodiphenyltrichloroethane (DDT) is a persistent organic pollutant (POP) and an organochlorine, which causes significant health problems in humans. The stress caused by cadmium (Cd) and the toxicity of DDT have direct effects on the growth and yield of crop plants. Ultimately, the greater uptake and accumulation of DDT by edible plants affects human health by contaminating the food chain. The possible solution to this challenging situation is to limit the passive absorption of POPs into the plants. Calcium (Ca) is an essential life component mandatory for plant growth and survival. This study used impregnated Ca (BdCa) of benzenedicarboxylic acid (Bd) to relieve abiotic stress in plants of Brassica alboglabra. BdCa mitigated the deleterious effects of Cd and reduced DDT bioaccumulation. By increasing the removal efficacy (RE) up to 256.14%, BdCa greatly decreased pollutant uptake (Cd 82.37% and DDT 93.64%) and supported photosynthetic machinery (86.22%) and antioxidant enzyme defenses (264.73%), in applied plants. Exogenously applied Bd also successfully improved the antioxidant system and the physiochemical parameters of plants. However, impregnation with Ca further enhanced plant tolerance to stress. This novel study revealed that the combined application of Ca and Bd could effectively relieve individual and combined Cd stress and DDT toxicity in B. alboglabra.

Alleviation of cadmium phytotoxicity in triacontanol treated Coriandrum sativum L. by modulation of physiochemical attributes, oxidative stress biomarkers and antioxidative system.

Cadmium (Cd) is a hazardous metal that has a significant risk of transfer from soil to edible parts of food crops including shoots and seeds. Reduction of Cd accumulation is required to lower the risk of Cd exposure in humans and animals feeding on metal contaminated parts of such plants. Coriandrum sativum L. (coriander) exposed to Cd showed stress symptoms such as stunted growth, reduced photosynthetic activity and synthesis of chlorophyll pigments. Growth inhibition in Cd-treated plants was attributed to induction of oxidative stress as demonstrated by higher level of stress biomarkers such as electrolyte leakage, lipid peroxidation and hydrogen peroxide. Primary objective of the current study was to observe the ameliorative role of triacontanol (Tria) in Cd-stressed coriander seedlings. For this purpose, coriander seeds were primed with Tria concentrations of 5, 10, and 20 µmol L-1. Seedlings developed from Tria treated seeds exhibited reduced loss of photosynthetic pigments; mitigated oxidative stress caused by Cd, through improved efficacy of antioxidant machinery comprising superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT) enzymes besides non-enzymatic antioxidants including proline, phenolics and flavonoids. Triacontanol treated seedlings showed enhanced yield attributes suggesting that exogenous Tria could be employed to improve plant tolerance to Cd stress.

Role of exogenously applied putrescine in amelioration of cadmium stress in Coriandrum sativum by modulating antioxidant system.

Abiotic stress reduces the plant growth and biomass production. Putrescine (Put) may be applied to alleviate numerous types of abiotic stresses in plants. The present research was intended to evaluate the role of exogenously applied Put in extenuation of cadmium (Cd) stress in coriander plants. Coriander seeds primed with 0.25, 0.5, and1 mM Put were allowed to grow in 50 mg kg-1 Cd contaminated soil for one month. Put treatment improved seed germination, gas exchange attributes, root growth and shoot growth of coriander. The improved activity of stress-responsive enzymes such as superoxide dismutase, catalase and peroxidase, besides amplification of proline was observed in Put treated seedlings under Cd stress. In addition, a reduced amount of total soluble protein and sugars content were noticed in Cd stressed seedlings. Nevertheless, Put reduced MDA level in treated plants. Our results demonstrated that Put mitigated Cd induced stress by modulating antioxidants and photosynthetic activity of coriander plants.Novelty statement Most of the researchers have studied the role of endogenous putrescine in alleviation of plant stress. However, during current study, we primed coriander seeds with putrescine. Our results elucidated very promising role of exogenously applied putrescine in stress mitigation and growth improvement of coriander seedlings under Cd stress. The findings of current study advocate the application of putrescine for stress alleviation in crop plants.

Titanium dioxide nanoparticles mitigate cadmium toxicity in Coriandrum sativum L. through modulating antioxidant system, stress markers and reducing cadmium uptake.

Anthropogenic activities are the foremost reason of metal pollution in soils of the cultivated areas, resulting abnormal physiochemical processes in plants. Among metals contaminants, cadmium (Cd) is one of the most injurious contaminants that deleteriously affect physiological activities, growth and yield of the crop plants. Keeping in view the stress mitigation potential of titanium dioxide (TiO2), the existing research work was premeditated to inspect the beneficial role of seed priming with titanium dioxide nanoparticles (TiO2-NPs) on biochemical, morphological and physiological characteristics of Coriandrum sativum L. (coriander) plants under Cd stress. For this purpose, C. sativum seeds were primed with 0, 40, 80 and 160 mg L-1 TiO2-NPs. Cadmium stress triggered a significant decrease in chlorophyll a content (49%), chlorophyll b content (44%), photosynthetic rate (62%) and plant growth (51%) as compared with control. Tanium dioxide nanoparticles treated seedlings exhibited reduced Cd contents besides improved agronomic traits (seedlings biomass, number of seeds and yield). The TiO2-NPs treatment declined the magnitude of EL and MDA by 1.5 fold and 1.71 fold, respectively. Furthermore, TiO2-NPs diminished oxidative injuries in plants exposed to Cd stress. Additionally, TiO2-NPs enhanced the biosynthesis of osmatic regulators (proline) by 47% which helped in the mitigation of Cd persuaded toxicity in plants. Briefly, treatment of 80 mg L-1 TiO2-NPs perhaps ameliorates the deleterious influence of Cd stress and enhance the yield of coriander.

Selenium nanoparticles reduced cadmium uptake, regulated nutritional homeostasis and antioxidative system in Coriandrum sativum grown in cadmium toxic conditions.

Nanotechnology has become a valuable novel approach to manage several environmental challenges through providing innovative and effective solutions. Heavy metal stress is an important abiotic limiting factor. Seed priming with selenium (Se) alleviates various kinds of environmental stresses; yet, the potential of seed priming with selenium nanoparticles (SeNPs) under cadmium (Cd) stress for coriander crop has never been evaluated. This research work was designed to explore the effects of seed priming with three levels (0, 5, 10 and 15 mg L-1) of SeNPs solution on the physio-biochemical characteristics, nutrition, antioxidative defense system and growth of coriander under Cd stress. Cadmium toxicity reduced chlorophyll content, photosynthetic activity and growth of treated plants. Moreover, Cd stressed plants exhibited modulations in proline level, together with decreased water potential, and leaf osmotic potential. However, SeNPs increased growth attributes, chlorophyll content, total soluble sugars, leaf relative water content, and gas exchange parameters in treated plants which were conversely decreased by Cd toxicity. The seeds priming with SeNPs promoted antioxidant response by increasing catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POX) activity and safeguarding cellular structures through scavenging free radicals and reactive oxygen species. Furthermore, Cd stressed plants displayed an upper level of MDA (1.91 fold) while SeNPs improved membranous integrity through detoxification of hydrogen peroxide. Additionally, SeNPs enhanced nutrients contents (P, K, Ca, Mg, Zn), metal tolerance index and diminished Cd content in plants resulting in the improved growth and development of Cd affected coriander plants.

Synergistic application of silver nanoparticles and indole acetic acid alleviate cadmium induced stress and improve growth of Daucus carota L.

Cadmium (Cd) is one of the major hazardous elements that is very toxic to the health of both human and plants. The toxicity of Cd causes plants to suffer by disabling their overall physiological mechanisms. Therefore, present study was intended to investigate the synergistic role of AgNPs and IAA in improving the resilience against Cd toxicity and underlaying physiological and biochemical mechanisms in carrot (Daucus carota L.) plants. Also, the existence of genotypic variation for Cd tolerance in D. carota was also studied. The results revealed that Cd stress decreased plant growth attributes like root diameter, root length, root weight, shoot weight, shoot length, leaves fresh weight and leaves dry weight. Nonetheless, AgNPs and IAA mitigated Cd stress by detoxifying reactive oxygen species (ROS). Additionally, the application of AgNPs and IAA boosted plant growth through reducing the level of malondialdehyde (MDA). Enhancement in the activity of phenol synthesizing and oxidizing enzymes including peroxidase, polyphenol oxidase and phenylalanine ammonia-lyase was also observed by application of AgNPs and IAA. The increased activities of antioxidant enzymes including POX, PPO and PAL by the combined application of AgNPs and IAA advocate stress ameliorative role against Cd stress in plants. The enhanced Cd content was detected in the roots as compared to shoots of treated plants. Pre breed 22 was found as a Cd tolerant genotype.

Selenium seed priming enhanced the growth of salt-stressed Brassica rapa L. through improving plant nutrition and the antioxidant system.

Various abiotic stresses may affect the germination, growth, and yield of direct-seeded vegetable crops. Seed priming with effective antioxidant mediators may alleviate these environmental stresses by maintaining uniformity in seed germination and improving the subsequent health of developing seedlings. Salt-induced stress has become a limiting factor for the successful cultivation of Brassica rapa L., especially in Southeast Asian countries. The present study was performed to elucidate the efficacy of seed priming using selenium (Se) in mitigating salt-induced oxidative stress in turnip crops by reducing the uptake of Na+. In this study, we administered three different levels of Se (Se-1, 75 µmol L-1; Se-2, 100 µmol L-1; and Se-3, 125 µmol L-1) alone or in combination with NaCl (200 mM). Conspicuously, salinity and Se-2 modulated the expression levels of the antioxidant genes, including catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and ascorbate peroxidase (APX). The upregulated expression of stress-responsive genes alleviated salt stress by scavenging the higher reactive oxygen species (ROS) level. The stress ameliorative potential of Se (Se-2 = 100 µmol L-1) enhanced the final seed germination percentage, photosynthetic content, and seedling biomass production up to 48%, 56%, and 51%, respectively, under stress. The advantageous effects of Se were attributed to the alleviation of salinity stress through the reduction of the levels of malondialdehyde (MDA), proline, and H2O2. Generally, treatment with Se-2 (100 µmo L-1) was more effective in enhancing the growth attributes of B. rapa compared to Se-1 (75 µmo L-1) and Se-3 (125 µmo L-1) under salt-stressed and non-stressed conditions. The findings of the current study advocate the application of the Se seed priming technique as an economical and eco-friendly approach for salt stress mitigation in crops grown under saline conditions.

Liquiritoside Alleviated Pb Induced Stress in Brassica rapa subsp. Parachinensis: Modulations in Glucosinolate Content and Some Physiochemical Attributes.

Current research was conducted to explore the effects of liquiritoside on the growth and physiochemical features of Chinese flowering cabbage (Brassica rapa subsp. parachinensis) under lead (Pb) stress. Lead stressed B. rapa plants exhibited decreased growth parameters, chlorophyll, and carotenoid contents. Moreover, Pb toxicity escalated the synthesis of malondialdehyde (MDA), hydrogen peroxide (H2O2), flavonoids, phenolics, and proline in treated plants. Nevertheless, foliar application of liquiritoside mitigated Pb toxicity by decreasing oxidative stress by reducing cysteine, H2O2, and MDA contents in applied plants. Liquiritoside significantly increased plant height, shoot fresh weight and dry weight, number of leaves, and marketable value of Chinese flowering cabbage plants exposed to Pb toxicity. This biotic elicitor also enhanced the proline, glutathione, total phenolics, and flavonoid contents in Chinese flowering cabbage plants exposed to Pb stress compared with the control. Additionally, total glucosinolate content, phytochelatins (PCs), and non-protein thiols were effectively increased in plants grown under Pb regimes compared with the control plants. Overall, foliar application of liquiritoside can markedly alleviate Pb stress by restricting Pb translocation in Chinese flowering cabbage.

Silicon assisted ameliorative effects of iron nanoparticles against cadmium stress: Attaining new equilibrium among physiochemical parameters, antioxidative machinery, and osmoregulators of Phaseolus lunatus.

Currently, producing safe agricultural commodities from the crop plants cultivated in the soil with increasing heavy metal toxicity is a gigantic challenge in front of researchers. Heavy metals are absorbed and translocated in the crop plants and then transferred to every downstream consumer of the food chain, including humans, causing serious disorders and ailments. The current research presents a combined schematic application of iron nanoparticles (Fe-NPs) and/or silicon (Si), to mitigate cadmium (Cd) stress in Lima bean (Phaseolus lunatus). It was noted that Cd-induced toxicity curtailed growth, antioxidative machinery, glyoxalase system and nutrient uptake of the plants. Furthermore, the physiochemical features of Cd stressed plants, including carotenoids, chlorophyll, photochemical quenching, photosynthetic efficiency, and leaf relative water contents, were improved by the combined application of Si and Fe-NPs. Moreover, higher levels of malondialdehyde (MDA), methylglyoxal (MG), hydrogen peroxide (H2O2), and electrolyte leakage (EL) were observed in Cd stressed plants. Nevertheless, the independent treatment or combined application of Si and/or Fe-NPs attenuated the adversative effects of Cd on the aforementioned growth attributes. Furthermore, Si and Fe-NPs defended plants from the injurious effects of MG by improving the activities of the glyoxalase enzyme. The Si and Fe-NPs reduced Cd contents but at the same time improved uptake and accumulation of nutrients in treated plants exposed to the Cd regime. This study highlights that Si and Fe-NPs have enormous potential to mitigate Cd-induced phytotoxicity by declining Cd uptake and improving the growth attributes of plants if applied in combination.

Enhanced performance of Bacillus megaterium OSR-3 in combination with putrescine ammeliorated hydrocarbon stress in Nicotiana tabacum.

Hydrocarbon stress (HS) has been causing decreased plant growth and productivity. Putrescine (Put) and growth promoting microbes are vital for plant growth and development under hydrocarbon stress. Current research work was carried out to evaluate the potential of Bacillus megaterium OSR-3 alone and in combination with Put to alleviate HS in Nicotiana tabacum (L.). The crude petroleum contaminated soil significantly reduced growth attributes of N. tabacum. B. megaterium OSR-3 inoculated plants subjected to HS exhibited improved photosynthetic rate, gas exchange characteristics, poline contents and protein level. Furthermore, bacterial inoculation enhanced the antioxidative activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in tobacco plants subjected to HS. The HS alleviation in B. megaterium OSR-3 inoculated N. tabacum can be credited to the heightened activity of antioxidative enzymes, reduction in hydrogen peroxide (H2O2) and abridged synthesis of malondialdehyde (MDA). The increased synthesis of indole acetic acid (IAA) in HS stressed N. tabacum plants treated with co-application of B. megaterium OSR-3 and Put attenuated toxicity and amplified growth of plants. Additionally, the co-application of B. megaterium OSR-3 and Put also upregulated the activity of antioxidative enzymes and induced augmented level of proline and IAA in plants under HS regimes. Current research provides novel insight into the potential and mechanism of B. megaterium OSR-3 and Put in mitigation of HS in N. tabacum plants.

Hydrogen sulfide mitigates cadmium induced toxicity in Brassica rapa by modulating physiochemical attributes, osmolyte metabolism and antioxidative machinery.

Hydrogen sulfide (H2S) is helpful for maintaining plant growth under abiotic stresses. The current study elucidated the physiological and biochemical strategies by which sodium hydrosulfide (NaHS), a donor of H2S, alleviated cadmium (Cd) toxicity in Brassica rapa. B. rapa plants growing under 50 mgkg-1 Cd stress showed reduced leaf relative water contents (LRWC), photosynthetic pigments, total soluble proteins, minerals uptake, antioxidants and growth. Furthermore, enhanced accumulation of Cd contents caused augmentation in levels of electrolyte leakage (EL) and methylglyoxal (MG). Nevertheless, improved physiochemical parameters in B. rapa seedlings obtained from seeds primed with 1.5 mM NaHS resulted better phenotype, growth and biomass production in Cd stressed plants. Protective stimulus of H2S regulated minerals and Cd homeostasis besides increased activity of antioxidants which decreased level of reactive oxygen species (ROS), EL, malondialdehyde (MDA) and MG in Cd regimes. Furthermore, H2S treated seedlings exhibited reduction in Cd content and revealed an active participation in the indole acetic acid (IAA) mediated pathway during stress. The findings of current study propose that H2S improved stress tolerance and mitigated Cd stress in B. rapa by modulating growth biomarkers and antioxidative system.

Combined effect of Bacillus fortis IAGS 223 and zinc oxide nanoparticles to alleviate cadmium phytotoxicity in Cucumis melo.

Cadmium (Cd), prevailing in most of the agricultural lands of the world contaminates food chain, thereby causing several health implications. It has become the main heavy metal contaminant in most of the agricultural lands of Pakistan due to the widespread use of phosphate fertilizers besides application of irrigation water contaminated with industrial and mining effluents. Plant growth promoting bacteria (PGPB) are capable to enhance growth and metal stress tolerance in supplemented plants. Zinc oxide nanoparticles (ZnO-NPs) are capable to alleviate various abiotic stresses when applied to plants. During current research, the efficacy of single and combined application of Bacillus fortis IAGS 223 and ZnO-NPs was evaluated for alleviation of Cd (75 mg kg-1) induced phytotoxicity in Cucumis melo plants. For this purpose, C. melo plants, subjected to Cd stress were treated with B. fortis IAGS 223 and ZnO-NPs (20 mg kg-1), either alone or in combination. The growth relevant characteristics including photosynthetic pigments, hydrogen peroxide (H2O2), malondialdehyde (MDA), and activities of antioxidative enzymes as well as Zn and Cd contents in treated plants were examined. The individual application of ZnO-NPs and B. fortis IAGS 223 slightly enhanced all the above-mentioned growth characteristics in plants under Cd stress. However, the combined application of ZnO-NPs and B. fortis IAGS-223 considerably modulated the activity of antioxidant enzymes besides upgradation of the biochemicals and growth parameters of Cd stressed plants. The decreased amount of stress markers such as H2O2, and MDA in addition with reduction of Cd contents was observed in shoots of ZnO-NPs and B. fortis IAGS-223 applied plants. B. fortis IAGS-223 inoculated plants supplemented with ZnO-NPs, exhibited reduced amount of Cd as well as protein bound thiols and non-protein bound thiols under Cd stress. Subsequently, the reduced Cd uptake improved growth of ZnO-NPs and B. fortis IAGS-223 applied plants. Henceforth, field trials may be performed to formulate appropriate combination of ZnO-NPs and B. fortis IAGS-223 to acquire sustainable crop production under Cd stress.

Synergistic effects of nitric oxide and silicon on promoting plant growth, oxidative stress tolerance and reduction of arsenic uptake in Brassica juncea.

Arsenic (As) polluted food chain has become a serious issue for the growth and development of humans, animals and plants. Nitric oxide (NO) or silicon (Si) may mitigate As toxicity. However, the combined application of NO and Si in mitigating As uptake and phytotoxicity in Brassica juncea is unknown. Hence, the collegial effect of sodium nitroprusside (SNP), a NO donor and Si application on B. juncea growth, gas exchange parameters, antioxidant system and As uptake was examined in a greenhouse experiment. Arsenic toxicity injured cell membrane as signposted by the elevated level of malondialdehyde (MDA) and hydrogen peroxide (H2O2), thus decreasing the growth of stressed plants. Moreover, As stress negatively affected gas exchange parameters and antioxidative system of plants. However, NO or/and Si alleviated As induced oxidative stress through increasing the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione S-transferase (GST), glutathione (GSH), along with thiol and proline synthesis. Furthermore, plants treated with co-application of NO and Si showed improved growth, gas attributes and decreased As uptake under As regimes. The current study highlights that NO and Si synergistically interact to mitigate detrimental effects of As stress through reducing As uptake. Our findings recommend combined NO and Si application in As spiked soils for improvement of plant growth and stress alleviation.

Synergistic Effect of Bacillus thuringiensis IAGS 199 and Putrescine on Alleviating Cadmium-Induced Phytotoxicity in Capsicum annum.

Plant growth-promoting bacteria (PGPB) and putrescine (Put) have shown a promising role in the mitigation of abiotic stresses in plants. The present study was anticipated to elucidate the potential of Bacillus thuringiensis IAGS 199 and Put in mitigation of cadmium (Cd)-induced toxicity in Capsicum annum. Cadmium toxicity decreased growth, photosynthetic rate, gas exchange attributes and activity of antioxidant enzymes in C. annum seedlings. Moreover, higher levels of protein and non-protein bound thiols besides increased Cd contents were also observed in Cd-stressed plants. B. thuringiensis IAGS 199 and Put, alone or in combination, reduced electrolyte leakage (EL), hydrogen peroxide (H2O2) and malondialdehyde (MDA) level in treated plants. Synergistic effect of B. thuringiensis IAGS 199 and Put significantly enhanced the activity of stress-responsive enzymes including peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD). Furthermore, Put and microbial interaction enhanced the amount of proline, soluble sugars, and total soluble proteins in C. annum plants grown in Cd-contaminated soil. Data obtained during the current study advocates that application of B. thuringiensis IAGS 199 and Put establish a synergistic role in the mitigation of Cd-induced stress through modulating physiochemical features of C. annum plants.

Butanolide alleviated cadmium stress by improving plant growth, photosynthetic parameters and antioxidant defense system of brassica oleracea.

Current study was performed to explore the effect of butanolide (KAR1) in mitigation of cadmium (Cd) induced toxicity in Brussels sprout (Brassica oleracea L.). Brussels sprout seeds, treated with 10-5 M, 10-7 M and 10-10 M solution of KAR1 were allowed to grow in Cd-contaminated (5 mg L-1) regimes for 25 d. Cadmium toxicity decreased seed germination and growth in B. oleracea seedlings. Elevated intensity of electrolyte leakage (EL), malondialdehyde (MDA) and hydrogen peroxide (H2O2) were observed in Cd-stressed seedlings. Additionally, reduced level of stomatal conductivity, transpiration rate, photosynthesis rate, intercellular carbon dioxide concentration, and leaf relative water content (LRWC) was also observed in Cd-stressed seedlings. Nevertheless, KAR1 improved seed germination, seedling growth and biomass production in Cd stressed plants. KAR1 application showed elevated LRWC, osmotic potential, and higher membranous stability index (MSI) in seedlings under Cd regime. Furthermore, seedlings developed by KAR1 treatment exhibited higher stomatal conductivity, and intercellular carbon dioxide concentration together with improved rate of transpiration and photosynthetic rate in B. oleracea under Cd stress. These findings elucidate that the reduced level of MDA, EL and H2O2, as well as improvement in antioxidative machinery increased growth and alleviated Cd toxicity in KAR1 treated seedlings under Cd stress.

Synergistic ameliorative effect of iron oxide nanoparticles and Bacillus subtilis S4 against arsenic toxicity in Cucurbita moschata: polyamines, antioxidants, and physiochemical studies.

The present study was intended to assess the potential of iron oxide nanoparticles (IONPs) and Bacillus subtilis S4 in mitigation of arsenic (As) stress in Cucurbita moschata. Cucurbita moschata seedlings were subjected to As stress for 60 days. Reduced level of growth parameters including photosynthetic pigments, rate of photosynthesis and gas exchange characteristics was observed in seedlings subjected to As stress. However, IONPs and B. subtilis S4 improved growth attributes and proline contents in supplemented C. moschata seedlings. Bacillus subtilis S4 inoculated seedlings showed higher activity of peroxidase (POD) and superoxide dismutase (SOD) under As toxicity. Similarly, the co-application of IONPs and B. subtilis S4 further increased the activity of these antioxidative enzymes. The As stress alleviation in inoculated C. moschata seedlings is credited to reduced levels of hydrogen peroxide (H2O2), malondialdehyde (MDA) and electrolyte leakage (EL) in IONPs and B. subtilis S4-treated plants. Furthermore, synergism between plant growth promoting bacteria (PGPB) and IONPs enhanced the biosynthesis of stress mitigating polyamines including spermidine and putrescine in As-stressed seedlings. Current research reveals that synergistic application of IONPs and B. subtilis S4 is an effective sustainable and ecofriendly approach for alleviation of As stress in C. moschata seedlings.