Do changes in the frailty score differ by the type of group sports and exercises participated in? A 3-year longitudinal study.

BACKGROUND: Older adults who engage in group sports and exercises achieve greater health benefits than those who exercise by themselves. The benefits of group participation may vary depending on the type of sports/exercise they engage in. The present study aimed to identify the association between specific sports and exercise types performed in groups and evaluate the longitudinal changes in multidimensional frailty scores among community-dwelling older adults in Japan. METHODS: We used 3-year follow-up data from the Japan Gerontological Evaluation Study and analyzed 33,746 men and 36,799 women aged ≥ 65 years. To elucidate the relationship between participation in 20 types of group sports/exercises in 2016 (baseline) and the change in frailty score (using the Kihon Checklist, KCL) from 2016 to 2019, we performed linear regression analyses through multivariate adjustments for age group, self-rated health, marital status, living alone, occupational status, years of education, alcohol drinking status, smoking status, equivalent income, and disease status using an inverse probability weighting method. P < 0.05 was considered statistically significant. RESULTS: The mean change in KCL scores over 3 years was + 0.62 and + 0.61 points in men and women, respectively, implying the degree of frailty worsened. The sports/exercise types that significantly prevented increments in KCL scores for both sexes compared to non-participants were hiking (men: B, - 0.36; women: B, - 0.29), walking (men: B, - 0.26; women: B, - 0.24), tennis (men: B, - 0.23; women: B, - 0.24), ground golf (men: B, - 0.21; women: B, - 0.19), and weight exercises (men: B, - 0.19; women: B, - 0.16). CONCLUSION: Participation in specific sports and exercise groups offer significant physical and psychological benefits for frailty prevention among older adults in Japan. The results of this study may offer substantive evidence to encourage older adults to participate in group activities for the prevention of multidimensional frailty. It will also help public health stakeholders to decide which type of sports and exercise groups to promote in a community.

Correlates of engaging in sports and exercise volunteering among older adults in Japan.

This study aimed to identify factors associated with engaging in sports and exercise volunteering among older adults. We used cross-sectional data from the Japan Gerontological Evaluation Study (JAGES), a nationwide mail survey of 20,877 older adults from 60 municipalities. Multilevel mixed-effects logistic regression analysis was used to investigate the correlation between engaging in sports and exercise volunteering and 39 variables classified into five factors: (1) demographic and biological, (2) behavioral, (3) psychological, cognitive, and emotional, (4) social and cultural, and (5) environmental factors. Among the analyzed samples, 1580 (7.6%) participants volunteered a few times/year or more often. Factors that showed positive association with the volunteering were older age, a current drinking habit, excellent self-rated health, high proportion of sports group participants in a living area, low municipal population density, and rich social and cultural features (i.e., social cohesion, support, network, and participation). Meanwhile, those that had a negative association were women, low level of education, deteriorated instrumental activities of daily living, having a past or current smoking habit, poor self-rated health, and depressive symptoms. We clarified the characteristics of the population that is more likely to participate in sports and exercise volunteering as well as those of the population that is less likely to participate and requires support.

[Does participation in community gatherings suppress aggravation of functional decline risk among older people? A study based on 2013-2016 data from the Japan Gerontological Evaluation Study].

Objective　Through the amendment of the Long-Term Care Insurance Law in 2014, Japan's Ministry of Health, Labour and Welfare established a general long-term care and prevention project centered on "Kayoinoba" to promote participation in social and physical activities for older people, which included environmental approaches for individual health and well-being through community-building. However, reports show that the effectiveness of long-term care and prevention in Kayoinoba across multiple municipalities is limited. The purpose of this study was to verify the effect of participation in Kayoinoba in reducing the risk of functional decline among older people, using data from 24 municipalities of 10 prefectures nationwide.Methods　This study examined self-administered mail survey data from the Japan Gerontological Evaluation Study. The participants were older people aged ≥65 years who lived in 24 municipalities of 10 prefectures, in 2013 and 2016. The dependent variable was an increase in total score of ≥5 points on a risk assessment scale predicting incident functional disability ("incident functional disability risk score")(Tsuji et al., 2018), and the explanatory variable was existence of participation in a Kayoinoba program. Nine variables were used as the covariates: educational attainment, equivalent income, depression, smoking, drinking, instrumental activities of daily living, incident functional disability risk score in 2013 (including sex and age), living status (whether the person lived alone), and employment status in 2013. We conducted Poisson regression analysis with stratification of the participants into two groups according to age: young older people and old older people. Sensitivity analysis of the possible increase of ≥3 or 7 points in the incident functional disability risk score was also conducted.Results　Of the 3,760 participants in the study, 472 (316 young older people and 156 old older people)[12.6% (11.8%, 14.5%)] participated in Kayoinoba. Compared with those who did not participate in Kayoinoba, the incidence rate ratio (IRR) of increase in risk assessment score was 0.88 (95% confidence interval: 0.65-1.18) for all who did participate, 1.13 (0.80-1.60) in the young older people and 0.54 (0.30-0.96) in the old older people, and was significant in the latter. In addition, similar results were obtained in the sensitivity analysis with the dependent variable as an increase in total score of ≥3 or 7 points on the risk assessment scale predicting incident functional disability.Conclusions　Compared with those who did not participate in Kayoinoba, functional decline risk was suppressed in those who did participate. The IRR was suppressed 46% in old older people. Promoting participation in Kayoinoba may effectively prevent the need for long-term care in old older people.

Acidic xylooligosaccharide preserves hepatic iron storage level in adult female rats fed a low-iron diet.

Iron deficiency anemia (IDA) is one of the most serious forms of malnutrition. This experiment was conducted to investigate whether acidic xylooligosaccharide (U-XOS), expected to have a high iron bioavailability, was useful in the prevention of iron deficiency. Experiment 1: Nineteen female Sprague-Dawley rats (20 wk old) were fed three different diets for 28 d; a U-XOS-supplemented low-iron diet (LI-X, n=7), a low-iron diet (LI, n=6), and a control diet (C, n=6). On day 28, the LI-X and LI groups showed iron deficiency without anemia. A significant difference in the total and unsaturated iron binding capacity, and serum transferrin saturation level was shown in the LI-X and LI groups, compared with the C group. However, the decrease of hepatic iron content of the LI-X group was suppressed compared with the LI group. Experiment 2: Eleven male Sprague-Dawley rats (7 wk old) were fed a U-XOS-supplemented diet (X, n=5) or a control diet (C, n=6) for 7 d. No significant difference in body weight gain or food intake was demonstrated between the two groups; the apparent iron absorption rate of the X group increased clearly compared with that of the C group. These results suggested that a U-XOS diet could preserve storage of hepatic iron in adult female rats fed a low-iron diet and could prevent IDA by promotion of dietary iron absorption, inhibition of iron excretion, and/or improvement of iron bioavailability.

Actin and Vimentin proteins with N-terminal deletion detected in tumor-bearing rat livers induced by intraportal-vein injection of Ha-ras-transfected rat liver cells.

The introduction of the tumorigenic v-Ha-ras oncogene-transformed rat liver epithelial cells (WBras), which is deficient in gap junctional intercellular communication (GJIC), into F344 rats, induces significant formation of hepatocellular tumors. GJIC plays a major role in maintaining tissue homeostasis. Using this in vivo tumor model system, we used 2-dimensional electrophoresis with isoelectric focusing in the first dimension and SDS-PAGE in the second dimension to globally identify proteins that are uniquely expressed in the livers of WBras-treated rats as compared to the sham control. Immunoblotting was used to identify Ras and Connexin43, which were the positive and negative marker proteins, respectively, of the introduced WBras cells. As predicted, immunoblotting indicated that the whole liver of tumor-bearing animals exhibited a decreased level of Connexin43 and an increased level of Ras. Connexin43 and GJIC were expressed and functional in normal liver, but not in the tumor. In addition to these 2 markers, an additional 4 proteins exhibited decreased levels and 2 proteins exhibited increased levels in the livers of tumor-bearing animals. N-Terminal sequencing analysis was used to identify these proteins, which were glucose-regulated protein 78, 2 isoforms of heat shock protein 60, and the beta-chain of ATP synthase for the down regulated proteins, and beta-Actin with a 46 amino acid deletion from its N-terminus and Vimentin with a 71 amino acid deletion from its N-terminus for the up regulated proteins. These data offer potentially new markers of liver tumorigenicity, particularly, Vimentin. (