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1.
Clin Orthop Surg ; 15(1): 42-49, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36778997

RESUMO

Background: Management of periprosthetic femoral fractures (PFFs) is reportedly challenging. Different patterns of PFFs would occur based on whether stem fixation was primarily cemented or cementless and whether these patterns would be associated with clinical outcomes, such as subsidence, fracture union, and complications, after stem revision. Methods: A retrospective comparative study was performed, involving 52 PFF patients treated with tapered fluted modular stems (TFMSs). In the 52 patients with Vancouver B2 or B3, including 21 cemented stems and 31 cementless stems, fracture patterns and bone stock were analyzed. Clinical outcomes after revision surgery using the TFMSs were compared between the two groups. Results: Transverse or short oblique type PFFs occurred around the cemented stem with loosening at the bone-cement interface. The Paprosky type III femoral deficiency and Vancouver type B3 fracture were observed more frequently in the cemented stem group. Otherwise, spiral fractures occurred more frequently in the cementless group (p < 0.001). Excessive subsidence of > 5 mm was observed more frequently in the cemented stem group (p < 0.001). The re-revision rates were higher in the cemented group than in the cementless group (p = 0.047). Conclusions: In our study, it was found that the patterns of transverse or oblique PFFs were more frequently produced with cemented stems, while long spiral fractures were more frequent with cementless stems. Stem subsidence and reoperation related to complications were more common in patients with PFFs around cemented stems than those with PFFs around cementless stems.


Assuntos
Artroplastia de Quadril , Fraturas do Fêmur , Prótese de Quadril , Fraturas Periprotéticas , Humanos , Artroplastia de Quadril/efeitos adversos , Artroplastia de Quadril/métodos , Fraturas Periprotéticas/cirurgia , Fraturas Periprotéticas/etiologia , Prótese de Quadril/efeitos adversos , Estudos Retrospectivos , Desenho de Prótese , Fraturas do Fêmur/cirurgia , Reoperação/métodos
2.
Int J Biol Macromol ; 227: 641-653, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36549614

RESUMO

A cavity defect inside the bone is formed by deformed cancellous bone from the fixation of the cortical bone, and consequently, abnormal bone healing occurs. Therefore, repairing cancellous bone defects is a remarkable topic in orthopedic surgery. In this study, we prepared bone marrow-derived stem cell (BMSC)-laden and bone morphogenetic protein-2 (BMP-2)-laden visible light-cured carboxymethyl chitosan (CMCS) hydrogels for cortical and cancellous bone healing. Proton nuclear magnetic resonance (1H NMR) analysis confirmed the methacrylation of CMCS (CMCSMA), resulting in 55 % of substitution. The higher concentration of CMCSMA hydrogel resulted in the lower swelling ratio, the larger viscosity, the slower degradation behavior, and the stronger compressive strength. The 5 w/v% hydrogel exhibited a controlled BMP-2 release for 14 days, while the 7 and 10 w/v% hydrogels displayed a controlled BMP-2 release for 28 days. Results of in vitro cytotoxicity and cell proliferation assays revealed the biocompatibility of the samples. In vivo animal tests demonstrated that BMSC- and BMP-2-laden 7 w/v% CMCSMA (CMCSMA+Cell+BMP-2) improved bone formation in the defected cortical and cancellous bones of the femur, as analyzed by micro-computed tomography (micro-CT) and histological evaluations. Consequently, we suggested that CMCSMA+Cell+BMP-2 can be a valuable scaffold for restoring cortical and cancellous bone defects.


Assuntos
Quitosana , Hidrogéis , Células-Tronco , Animais , Proteína Morfogenética Óssea 2/farmacologia , Proteína Morfogenética Óssea 2/química , Osso Esponjoso , Quitosana/química , Hidrogéis/farmacologia , Hidrogéis/química , Luz , Microtomografia por Raio-X , Medula Óssea
3.
Medicina (Kaunas) ; 58(10)2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36295657

RESUMO

Background: Position-related compressive nerve injury is a frequently reported complication of the lithotomy position. In contrast, compartment syndrome-induced neuropathy after lithotomy with prolonged surgery is rare and prone to misdiagnosis. This case describes the successful open decompression of sciatic neuropathy due to compartment syndrome after a prolonged lithotomy position. Case presentation: A 56-year-old male patient complained of an abnormal sensation in the lower leg and difficulty in dorsiflexion and plantarflexion of the left foot and toes after laparoscopic anterior hepatic sectionectomy for 16 h in a lithotomy position. Physical examination revealed severe pain and paresthesia below the distal left thigh. In manual muscle test grading, dorsiflexion and plantarflexion of the left ankle and toes were classified as grade 1. Computed tomography and magnetic resonance imaging showed ischemic changes in the mid-thigh posterior muscles, and the sciatic nerve was severely swollen at the distal thigh, which was compressed by the proximal edge of the well-leg holder. After debridement of the necrotic tissue and decompression of the sciatic nerve, the pain subsided immediately, and the ankle and toe dorsiflexion motor function improved to grade 4. Conclusions: Most case reports of compressive neuropathy associated with the lithotomy position have been related to conservative treatment. However, if a lesion compressing the nerve is confirmed in an imaging study and the correlation with the patient's symptoms is evident, early surgical intervention can be an effective treatment method to minimize neurological deficits.


Assuntos
Síndromes Compartimentais , Neuropatia Ciática , Masculino , Humanos , Pessoa de Meia-Idade , Síndromes Compartimentais/etiologia , Síndromes Compartimentais/cirurgia , Síndromes Compartimentais/patologia , Nervo Isquiático/patologia , Nervo Isquiático/cirurgia , Neuropatia Ciática/etiologia , Neuropatia Ciática/cirurgia , Dor , Descompressão/efeitos adversos
4.
World J Clin Cases ; 10(25): 9028-9035, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36157671

RESUMO

BACKGROUND: A large ganglionic cyst extending from the hip joint to the intrapelvic cavity through the sciatic notch is a rare space-occupying lesion associated with compressive lower-extremity neuropathy. A cyst in the pelvic cavity compressing the intrapelvic-sciatic nerve is easily missed in the diagnostic process because it usually presents as atypical symptoms of an extraperitoneal-intrapelvic tumor. We present a case of a huge ganglionic cyst that was successfully excised laparoscopically and endoscopically by a gynecologist and an orthopedic surgeon. CASE SUMMARY: A 52-year-old woman visited our hospital complaining of pain and numbness in her left buttock while sitting. The pain began 3 years ago and worsened, while the numbness in the left lower extremity lasted 1 mo. She was diagnosed and unsuccessfully treated at several tertiary referral centers many years ago. Magnetic resonance imaging revealed a suspected paralabral cyst (5 cm × 5 cm × 4.6 cm) in the left hip joint, extending to the pelvic cavity through the greater sciatic notch. The CA-125 and CA19-9 tumor marker levels were within normal limits. However, the cyst was compressing the sciatic nerve. Accordingly, endoscopic and laparoscopic neural decompression and mass excision were performed simultaneously. A laparoscopic examination revealed a tennis-ball-sized cyst filled with gelatinous liquid, stretching deep into the hip joint. An excisional biopsy performed in the pelvic cavity and deep gluteal space confirmed the accumulation of ganglionic cysts from the hip joint into the extrapelvic intraperitoneal cavity. CONCLUSION: Intra- or extra- sciatic nerve-compressing lesion should be considered in cases of sitting pain radiating down the ipsilateral lower extremity. This large juxta-articular ganglionic cyst was successfully treated simultaneously using laparoscopy and arthroscopy.

5.
J Clin Med ; 11(9)2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35566596

RESUMO

Dysphagia is the most common complication of anterior cervical discectomy and fusion (ACDF). Several studies have reported dysphagia's incidence, severity, and prognosis after ACDF; however, few have investigated the objective effects of dysphagia management. We aimed to elucidate the efficacy of laryngeal rehabilitation therapy for dysphagia following ACDF. This prospective randomized control trial included 20 patients who underwent more than two-level ACDF. Laryngeal rehabilitation therapy was performed on 10 patients for 7 days, whereas the remaining 10 comprised the control group. Pharyngeal transit time (PTT) by videofluoroscopic swallowing study (VFSS) was performed to evaluate the objective state of swallowing. We analyzed Bazaz scale and total variance of prevertebral soft tissue swelling (PSTS) from C2 to C7 on lateral cervical radiographs during hospitalization and at 4 and 8 weeks post-surgery. The PTT of the rehabilitation group was shorter than that of the control group at 7 days and 4 weeks post-surgery (p-value; POD 7D = 0.003, POD 4W = 0.042, POD 8W = 0.097). Perioperative laryngeal rehabilitation therapy effectively reduces postoperative dysphagia after ACDF.

6.
Hip Pelvis ; 32(3): 148-155, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32953707

RESUMO

PURPOSE: The utility of total hip arthroplasty (THA) for the treatment of displaced femoral neck fractures in elderly patients (≥80 years of age) remains controversial as a patient's general condition is an essential factor impacting recovery. This study aims to determine if THA is a valuable option for appropriately selected elderly patients. MATERIALS AND METHODS: All patients underwent cementless THA using a direct lateral approach with a rectangular stem. Eighty-two patients ≥80 years of age underwent THA due to a displaced femoral neck. Clinical frailty scale (CFS) scores <5 were indicated for THA. The modified Harris hip score (mHHS), visual analogue scale (VAS), and patient satisfaction were used to assess outcomes. RESULTS: Nine of 82 patients died in the study period with another underlying disease. One, a 90-year-old male with pneumonia expired in the intensive care unit at 7-day postoperatively, while the other eight died due to causes unrelated to THA. Of the remaining 73 patients: (i) mean mHHS score increased to 80.57±21.36 at 1-year postoperatively; (ii) VAS was 2.3±0.9 points six-months postoperatively; and (iii) 78.7% of patients reported that they were very satisfied or satisfied 1-year postoperatively. The number of perioperative complications was 10.8% (9 hips) without the need for revision surgery. CONCLUSION: The use of THA in patients ≥80 years of age with low CFS scores (<5) described here yielded favorable results and a relatively low rate of complications. However, a well-controlled comparative study or randomized trial is required to further refine selection criteria for THA in this patient population.

7.
Nanomaterials (Basel) ; 9(12)2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31766334

RESUMO

Osteosarcoma (OSA) is a difficult cancer to treat due to its tendency for relapse and metastasis; advanced methods are therefore required for OSA treatment. In this study, we prepared a local drug-delivery system for OSA treatment based on doxorubicin·hydrochloride (DOX·HCl)/cisplatin (CP)-loaded visible light-cured glycol chitosan (GC) hydrogel/(2-hydroxypropyl)-beta-cyclodextrin (GDHCP), and compared its therapeutic efficiency with that of DOX·HCl- and CP-loaded GC hydrogels (GD and GHCP). Because of diffusion driven by concentration gradients in the swollen matrix, the three hydrogels showed sustained releases of DOX·HCl and CP over 7 days, along with initial 3-h bursts. Results of in vitro cell viability and in vivo animal testing revealed that GDHCP had a stronger anticancer effect than GD and GHCP even though there were no significant differences. Body weight measurement and histological evaluations demonstrated that the drug-loaded GC hydrogels had biocompatibility without cardiotoxicity or nephrotoxicity. These results suggested that GDHCP could be a good platform as a local drug-delivery system for clinical use in OSA treatment.

8.
Hip Pelvis ; 31(2): 102-109, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31198777

RESUMO

PURPOSE: Hip arthroscopy has been considered for treating hip dysplasia; however, its efficacy is still a matter of controversy. Here, we report outcomes of patients with borderline dysplasia treated with a contemporary hip arthroscopy technique. MATERIALS AND METHODS: Forty-seven hips with borderline hip dysplasia were treated using hip arthroscopy. Patients underwent procedures to correct torn labrums or ligamentum teres with additional procedure on the acetabular capsule. Patient outcomes were assessed using visual analogue scale (VAS), modified Harris Hip Score (mHHS), Nonarthritic Hip Score (NAHS) and patient satisfaction. Risk factors for poor prognosis were also investigated. RESULTS: The mean follow up period was 25.9 months. At the last follow up, mean VAS score decreased from 6.1±1.6 to 3.5±2.8 (P=0.016). The mHHS and NHAS at the last follow up improved from 61.0±7.6 to 78.6±19.5 (P=0.001) and 62.1±7.5 to 80.0±18.5 (P=0.002), respectively. While significant improvement was observed in all patient reported outcome measures tested, 19 (40.4%) hips indicated that "the operation was unsatisfactory." The only factor shown to influence outcomes was preoperative VAS (i.e., worse scores potentially an indicator of poor outcomes). CONCLUSION: The results of the current study indicate that arthroscopic management may be beneficial for a subset of patients with borderline dysplasia; however, the dissatisfaction rate associated with this treatment approach may be as high as 40%. The poor preoperative pain score appears to be the sole indicator for poor outcomes.

9.
Clin J Sport Med ; 29(3): 203-208, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31033613

RESUMO

OBJECTIVE: The purpose of this study was to assess the endoscopic findings of the sciatic nerve and clinical outcomes of major traumatic sciatic nerve neuropathies after fracture or reconstructive surgery of the acetabulum with idiopathic deep gluteal syndrome (DGS) groups. DESIGN: Retrospective review of patient reports. SETTING: Level I trauma center of a tertiary university hospital. PARTICIPANTS: The study included 70 patients who consecutively underwent endoscopic sciatic nerve decompression. Patients who had previous fractures or reconstructive surgeries of the acetabulum were categorized as the major trauma group, whereas those without major trauma were categorized as the idiopathic group (45 patients) after a minimum of 24-months of follow-up period. MAIN OUTCOME MEASURES: The results were evaluated using the modified Harris Hip Score (mHHS), Hip outcome, and 12-Item Short Form Health Survey scores, respectively. RESULTS: In the major trauma group, all patients with sensory symptoms showed some degree of relief after the endoscopic sciatic nerve release. None of the patients with complete foot drop demonstrated complete improvement. Three patients with motor weakness without foot drop showed complete improvement in motor function. The mean mHHS increased from 61.5 ± 13.4 to 84.1 ± 8.1 (P = 0.031). In the idiopathic DGS group, the mean mHHS increased from 73.8 ± 10.3 to 94.4 ± 5.3 (P = 0.003). The Benson outcomes rating in the major trauma group was statistically lower than that in the idiopathic DGS group. CONCLUSION: Endoscopic release of the sciatic nerve after fractures or reconstructive surgeries could provide some improvements without complications. However, more favorable outcomes were observed in the idiopathic DGS group.


Assuntos
Acetábulo/cirurgia , Descompressão Cirúrgica , Fraturas Ósseas/complicações , Síndrome do Músculo Piriforme/etiologia , Procedimentos de Cirurgia Plástica/efeitos adversos , Nervo Isquiático/cirurgia , Ciática/etiologia , Adulto , Endoscopia , Feminino , Fraturas Ósseas/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Dor/etiologia , Estudos Retrospectivos , Nervo Isquiático/fisiopatologia , Resultado do Tratamento
10.
Cell Death Differ ; 26(11): 2358-2370, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30787391

RESUMO

Emerging evidence indicates that estrogen receptor (ER) α is an important modulator of bone homeostasis, which occurs partly by promoting osteoclast apoptosis. Sirtuin 6 (Sirt6) is an anti-aging molecule, and its deficiency in mice results in skeletal malformations associated with progeroid features. However, the effects of Sirt6 on ERα function in osteoclasts, and thus on aging- or estrogen deficiency-induced bone loss, have not been studied. Here, we show that myeloid-specific deletion of Sirt6 led to decreased ERα protein level and apoptotic cell death in preosteoclasts. Consequently, myeloid Sirt6 KO mice showed aggravated cancellous bone loss both with aging and following an ovariectomy compared to wild-type littermates. In contrast, Sirt6 transgenic mice were protected from ovariectomy-induced bone loss. Mechanistically, Sirt6 deacetylated ERα protein to prevent its proteasomal degradation, in which lysine 171 and lysine 299 were critical residues. Sirt6-mediated ERα stabilization promoted transcription of Fas ligand in preosteoclasts, resulting in apoptosis of osteoclasts. Finally, the level of Sirt6 in human preosteoclasts was correlated positively with bone density and ERα but negatively with age. In conclusion, our results suggest that deacetylation and upregulation of ERα by Sirt6 in preosteoclasts prevent bone loss by inhibiting osteoclast-mediated bone resorption. Activation of Sirt6 in preosteoclasts may provide a new therapeutic approach to attenuate osteoporosis in older or postmenopausal patients.


Assuntos
Reabsorção Óssea/patologia , Receptor alfa de Estrogênio/metabolismo , Osteoclastos/metabolismo , Osteoporose/patologia , Sirtuínas/metabolismo , Animais , Apoptose/fisiologia , Densidade Óssea/genética , Reabsorção Óssea/prevenção & controle , Linhagem Celular , Estrogênios/deficiência , Feminino , Fêmur/fisiologia , Células HEK293 , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Osteoclastos/citologia , Osteogênese/fisiologia , Osteoporose/prevenção & controle , Ovariectomia , Sirtuínas/genética
11.
Drug Deliv ; 25(1): 1664-1671, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30183420

RESUMO

Systemic drug delivery systems (SDDSs) for thyroid cancer treatment are associated with serious side effects including nausea, anorexia, and hair loss as a result of damage to normal tissues. In this study, we investigated the feasibility of a local DDS (LDDS) based on visible light-cured glycol chitosan (GC) hydrogel and doxorubicin⋅hydrochloride (DOX⋅HCl), called GC10/DOX, on thyroid cancer treatment in vivo. Visible light irradiation increased the storage modulus and swelling ratio of the GC10/DOX hydrogel precursor. The release of DOX⋅HCl from GC10/DOX exhibited two unique patterns comprising an initial burst within 18 hours, followed by a controlled and sustained release thereafter. In vitro cell viability testing showed that GC10/DOX had a greater antitumor effect than free DOX⋅HCl and GC10 hydrogel controls. In vivo, local injection of GC10/DOX near tumor tissue led to a superior antitumor effect compared with controls consisting of free DOX⋅HCl intravenously injected to the tail vein of thyroid cancer-bearing mouse and GC10 hydrogel subcutaneously injected near the tumor. Altogether, our results suggest that GC10/DOX may have clinical potential for thyroid cancer treatment.


Assuntos
Antibióticos Antineoplásicos/administração & dosagem , Quitosana , Doxorrubicina/administração & dosagem , Sistemas de Liberação de Medicamentos , Neoplasias da Glândula Tireoide/tratamento farmacológico , Animais , Linhagem Celular Tumoral , Quitosana/química , Humanos , Luz , Masculino , Camundongos , Camundongos Endogâmicos BALB C
12.
BMC Musculoskelet Disord ; 19(1): 184, 2018 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-29866097

RESUMO

BACKGROUND: Sciatic nerve injuries following total hip arthroplasty are disabling complications. Although degrees of injury are variable from neuropraxia to neurotmesis, mechanical irritation of sciatic nerve might be occurred by protruding hardware. This case shows endoscopic decompression for protruded acetabular screw irritating sciatic nerve, the techniques described herein may permit broader arthroscopic/endoscopic applications for management of complications after reconstructive hip surgery. CASE PRESENTATION: An 80-year-old man complained of severe pain and paresthesias following acetabular component revision surgery. Physical findings included right buttock pain with radiating pain to lower extremity. Radiographs and computed tomography imaging showed that the sharp end of protruded screw invaded greater sciatic foramen anterior to posterior and distal to proximal direction at sciatic notch level. A protruding tip of the acetabular screw at the sciatic notch was decompressed by use of techniques gained from experience performing endoscopic sciatic nerve decompression. The pre-operative pain and paresthesias resolved post-operatively after recovering from anesthesia. CONCLUSIONS: This case report describes the first documented endoscopic resection of the tip of the acetabular screw irritating sciatic nerve after total hip arthroplasty. If endoscopic resection of an offending acetabular screw can be performed in a safe and minimally invasive manner, one can envision a future expansion of the role of hip arthroscopic surgery in several complications management after total hip arthroplasty.


Assuntos
Acetábulo/cirurgia , Parafusos Ósseos/efeitos adversos , Descompressão Cirúrgica/métodos , Neuroendoscopia/métodos , Nervo Isquiático/cirurgia , Acetábulo/diagnóstico por imagem , Idoso de 80 Anos ou mais , Humanos , Masculino , Nervo Isquiático/diagnóstico por imagem
13.
J Am Acad Orthop Surg ; 26(8): e181-e190, 2018 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-29557799

RESUMO

INTRODUCTION: The restoration of range of motion after surgery in patients with femoroacetabular impingement syndrome is considered an important factor that improves clinical outcomes and determines the quality of life of patients, especially in the Asian population. METHODS: Arthroscopic femoroplasty was done in 109 Asian patients with cam impingement. The patients were retrospectively evaluated using range of motion and clinical outcomes (Harris Hip Score [HHS], visual analog scale score, Hip Outcome Score-Activity of Daily Living, and Hip Outcome Score-Sports subscales) between preoperative and postoperative conditions, with a follow-up period of >2 years. RESULTS: The increments of hip motion were an internal rotation of 10.1° (P < 0.05) at 3 postoperative months and an external rotation of 11.8° (P < 0.05) at approximately 6 postoperative months; both were maintained at 2 postoperative years. Mean clinical outcomes improved from 69.1 to 82.1 for HHS, from 6.0 to 2.1 for the visual analog scale score, and from 40.1 to 68.7 for the Hip Outcome Score-Sports Subscale at 6 postoperative months (P < 0.05) and were maintained at 2 years. Younger subjects (<45 years of age) displayed even greater improvements in the HHS and Hip Outcome Score-Activity of Daily Living/Sports Subscale than the other group (≥45 years of age) (P < 0.05). CONCLUSION: Patients who underwent arthroscopic femoroplasty showed improvements in internal rotation at 3 months postoperatively and external rotation at approximately 6 months postoperatively. In addition, younger age (<45 years and the restoration of rotational hip motion resulted in better clinical outcomes. LEVEL OF EVIDENCE: III a.


Assuntos
Artroscopia/métodos , Impacto Femoroacetabular/fisiopatologia , Fêmur/cirurgia , Articulação do Quadril/fisiopatologia , Procedimentos de Cirurgia Plástica/métodos , Amplitude de Movimento Articular/fisiologia , Adulto , Povo Asiático , Feminino , Impacto Femoroacetabular/etnologia , Impacto Femoroacetabular/cirurgia , Articulação do Quadril/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , República da Coreia , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento
14.
Mol Clin Oncol ; 7(5): 915-918, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29181188

RESUMO

Osteochondromas are usually extra-articular lesions originating from the metaphysis of long bones. Intra-articular osteochondromas may also occur, causing pain and discomfort and restricting the range of motion. Osteochondromas of the femoral neck are intra-articular lesions that are difficult to access for surgical resection, particularly when located posteriorly. We herein present a rare case of an intra-articular osteochondroma involving the posteroinferior aspect of the femoral neck associated with secondary synovial osteochondromatosis (SOC) of the hip joint in a 25-year-old woman. Determining the optimal treatment was difficult due to the high risk of avascular necrosis (AVN) following surgical excision. The patient was successfully treated with arthroscopic surgery and she remained in good condition at 2 years postoperatively, with a full range of motion of the hip joint, and without signs of limping, recurrence of the SOC, or AVN of the femoral head.

15.
Molecules ; 22(9)2017 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-28891961

RESUMO

Scarless wound healing is ideal for patients suffering from soft tissue defects. In this study, we prepared a novel wet dressing (ß-CD-ic-CUR/GC) based on the visible light-cured glycol chitosan (GC) hydrogel and inclusion complex between beta-cyclodextrin (ß-CD) and curcumin (CUR). We also evaluated its efficacy in the acceleration of wound healing as compared to that of CUR-loaded GC (CUR/GC). The conjugation of glycidyl methacrylate (GM) to GC for photo-curing was confirmed by ¹H-NMR measurement, and the photo-cured GC hydrogel was characterized by the analyses of rheology, swelling ratio, SEM and degradation rate. After visible light irradiation, the surface/cross-sectional morphologies and storage (G')/loss (G'') moduli revealed the formation of hydrogel with interconnected porosity. The dressing ß-CD-ic-CUR/GC exhibited a controlled release of 90% CUR in a sustained manner for 30 days. On the other hand, CUR/GC showed CUR release of 16%. ß-CD acted as an excipient in improving the water-solubility of CUR and affected the release behavior of CUR. The in vivo animal tests including measurement of the remaining unhealed wound area and histological analyses showed that ß-CD-ic-CUR/GC may have potential as a wet dressing agent to enhance soft tissue recovery in open fractures.


Assuntos
Curativos Hidrocoloides , Curcumina/farmacologia , Preparações de Ação Retardada/farmacologia , Hidrogéis/farmacologia , Ferida Cirúrgica/tratamento farmacológico , Cicatrização/efeitos dos fármacos , Animais , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Quitosana/química , Curcumina/química , Curcumina/farmacocinética , Preparações de Ação Retardada/síntese química , Preparações de Ação Retardada/farmacocinética , Preparações de Ação Retardada/efeitos da radiação , Liberação Controlada de Fármacos , Compostos de Epóxi/química , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Hidrogéis/síntese química , Hidrogéis/farmacocinética , Hidrogéis/efeitos da radiação , Luz , Metacrilatos/química , Camundongos , Camundongos Endogâmicos BALB C , Processos Fotoquímicos , Ferida Cirúrgica/patologia , Cicatrização/fisiologia , beta-Ciclodextrinas/química
16.
Int J Mol Sci ; 18(8)2017 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-28771196

RESUMO

The most ideal implant models in the dental and orthopedic fields to minimize the failure rate of implantation involve the improvement of osseointegration with host bone. Therefore, a focus of this study is the preparation of surface-modified titanium (Ti) samples of disc and screw types using dexamethasone (DEX) and/or growth and differentiation factor-5 (GDF-5), as well as the evaluation of their efficacies on bone formation in vitro and in vivo. X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and contact angle measurement were used to evaluate the surface chemical composition, surface morphology and wettability, respectively. The results showed that implant surfaces were successfully modified with DEX and/or GDF-5, and had rough surfaces along with hydrophilicity. DEX, GDF-5 or DEX/GDF-5 on the surface-modified samples were rapidly released within one day and released for 28 days in a sustained manner. The proliferation and bone formation of MC3T3-E1 cells cultured on pristine and surface-modified implants in vitro were examined by cell counting kit-8 (CCK-8) assay, as well as the measurements of alkaline phosphatase (ALP) activity and calcium deposition, respectively. MC3T3-E1 cells cultured on DEX/GDF-5-Ti showed noticeable ALP activity and calcium deposition in vitro. Active bone formation and strong osseointegration occurred at the interface between DEX/GDF-5-Ti and host bone, as evaluated by micro computed-tomography (micro CT) analysis. Surface modification using DEX/GDF-5 could be a good method for advanced implants for orthopaedic and dental applications.


Assuntos
Materiais Revestidos Biocompatíveis , Dexametasona , Fator 5 de Diferenciação de Crescimento , Heparina , Osteogênese/efeitos dos fármacos , Titânio , beta-Ciclodextrinas , Animais , Antígenos de Diferenciação/biossíntese , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Dexametasona/química , Dexametasona/farmacologia , Fator 5 de Diferenciação de Crescimento/química , Fator 5 de Diferenciação de Crescimento/farmacologia , Heparina/química , Heparina/farmacologia , Humanos , Camundongos , Coelhos , Titânio/química , Titânio/farmacologia , beta-Ciclodextrinas/química , beta-Ciclodextrinas/farmacologia
17.
Int J Mol Sci ; 18(7)2017 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-28686210

RESUMO

The management of severe acetabular bone defects in revision reconstructive orthopedic surgery is challenging. In this study, cyclic precalcification (CP) treatment was used on both nanotube-surface Ti-mesh and a bone graft substitute for the acetabular defect model, and its effects were assessed in vitro and in vivo. Nanotube-Ti mesh coated with hydroxyapatite/ß-tricalcium phosphate (HA/ß-TCP) was manufactured by an anodizing and a sintering method, respectively. An 8 mm diameter defect was created on each acetabulum of eight rabbits, then treated by grafting materials and covered by Ti meshes. At four and eight weeks, postoperatively, biopsies were performed for histomorphometric analyses. The newly-formed bone layers under cyclic precalcified anodized Ti (CP-AT) meshes were superior with regard to the mineralized area at both four and eight weeks, as compared with that under untreated Ti meshes. Active bone regeneration at 2-4 weeks was stronger than at 6-8 weeks, particularly with treated biphasic ceramic (p < 0.05). CP improved the bioactivity of Ti meshes and biphasic grafting materials. Moreover, the precalcified nanotubular Ti meshes could enhance early contact bone formation on the mesh and, therefore, may reduce the collapse of Ti meshes into the defect, increasing the sufficiency of acetabular reconstruction. Finally, cyclic precalcification did not affect bone regeneration by biphasic grafting materials in vivo.


Assuntos
Acetábulo/patologia , Hidroxiapatitas/farmacologia , Nanotubos/química , Titânio/farmacologia , Animais , Calcificação Fisiológica/efeitos dos fármacos , Cerâmica/farmacologia , Materiais Revestidos Biocompatíveis/farmacologia , Masculino , Nanotubos/ultraestrutura , Coelhos , Cicatrização/efeitos dos fármacos
18.
BMC Musculoskelet Disord ; 18(1): 40, 2017 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-28122599

RESUMO

BACKGROUND: The purpose of this study was to determine whether additional tension band wiring in the plate for traumatic disruption of symphysis pubis has clinical benefits. Therefore, outcomes and complications were compared between a plate fixation group and a plate with tension band wiring group. METHODS: We retrospectively evaluated 64 consecutive patients who underwent open reduction and internal fixation of the symphysis pubis by using a plate alone (n = 39) or a plate with tension band wiring (n = 25). All the patients were followed up for a minimum of 24 months (mean, 34.4 months; range, 26-39 months). Demographic characteristics, outcomes, movement of the metal works, complications, revision surgery, and Majeed functional score were compared. RESULTS: Significant screw pullout was relatively significantly more frequently found in the plate fixation group than in the plate with tension band wiring group (P = 0.009). In terms of the overall rate of all-cause revision surgery, including significant loosening, symptomatic hardware, and patient-requested hardware removal during follow-up period, the plate with tension band wiring group showed a significantly lower rate. CONCLUSION: Tension band wiring in combination with a symphyseal plate showed better radiological outcomes, a lower incidence of hardware loosening, and a lower rate of revision surgery than plate fixation alone. This technique would have some potential advantages in terms of avoiding significant movement of plate, symptomatic hardware failure, and revision surgery.


Assuntos
Placas Ósseas , Fios Ortopédicos , Diástase da Sínfise Pubiana/diagnóstico por imagem , Diástase da Sínfise Pubiana/cirurgia , Adolescente , Adulto , Idoso , Placas Ósseas/estatística & dados numéricos , Fios Ortopédicos/estatística & dados numéricos , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Sínfise Pubiana/diagnóstico por imagem , Sínfise Pubiana/cirurgia , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
19.
Hip Int ; 25(1): 61-6, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25264205

RESUMO

Cementation of a polyethylene liner into the well-fixed shell is a convenient option for revision total hip arthroplasty. We retrospectively reviewed 45 patients who had liner cementation to investigate the risk factors which gave rise to major complications and reoperation. Patients were observed for a minimum of 7 years (range 7.8-14 years). Relevant risk factors (age, BMI, surgical approach, previous cup size and position, types of coated surface) were assessed with Cox regression analysis. The mean Harris Hip Score was improved from 62.5 (range 57-68) preoperatively to 87.1 (range 70-97). A total of 7 hips (15.5%) had acetabular component loosening that was treated with reoperation. Prevalence of acetabular component loosening was statistically significantly higher in hydroxyapatite-coated group (5 of 13) than in the Ti-coated group (2 of 32, p = 0.015). All recurrent dislocations occurred in patients treated with a posterior approach. Diameter of the previous metal shell of below 54 mm showed a lower 10-year survival rate than those greater than 54 mm in diameter. PE liner cementation in stable metal cup is a useful alternative option for carefully selected patients. Pre-existing HA-coated cups as well as small sized cups were indicative of poor outcomes.


Assuntos
Acetábulo/cirurgia , Artroplastia de Quadril/métodos , Cimentação/métodos , Prótese de Quadril , Polietileno , Adulto , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Desenho de Prótese , Falha de Prótese , Reoperação , Fatores de Tempo , Resultado do Tratamento
20.
Arthroscopy ; 30(8): 957-63, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24835839

RESUMO

PURPOSE: The aim of this study was to assess complications related to arthroscopy for femoroacetabular impingement (FAI) and how these complications have changed as we have gained more experience with this procedure. METHODS: The authors reviewed 200 hips (197 patients). The average patient age was 44.64 years and the mean follow-up time was 28.2 months. All patients underwent hip arthroscopy in the supine position. Clinically, Modified Harris Hip Scores (MHHS) and patient satisfaction with outcome were used. We divided complications into 3 groups: Group 1 related to traction, group 2 related to surgical technique or implant failure, and group 3 related to outcomes. RESULTS: Clinically, the MHHS improved from 69.96 (±6.10) to 80.45 (±7.00), and patient satisfaction with the achieved outcome increased to 8.87 (±0.76). The overall complication rate was 15% (30 of 200 hips). Group 1 consisted of 4 patients with pudendal neuropraxia and 2 patients with ankle joint pain (P = .013). Group 2 consisted of 2 patients with lateral femoral cutaneous neuropraxia, 2 patients with iatrogenic labral perforations, one patient with a labral tear, and 4 patients with femoral head scuffs. There were 4 incidents of instrument breakage. Furthermore, 3 suture anchors failed, a second-degree burn occurred in one patient, and there was incomplete reshaping in 5 hips (P = .045). Group 3 included one patient with a snapping sound and heterotopic ossification. Second-look arthroscopy was performed for 5 hips. All the complications outlined in groups 1 and 2 are related to the learning curve and have statistical significance (P < .05). CONCLUSIONS: Complications relating to hip arthroscopy took different forms during the early learning period, but overall complication rates decreased along the learning curve. Surgical technique-related complications such as problems with suture anchors and the reshaping of cam impingements were also considered during the later stage. LEVEL OF EVIDENCE: Level IV, therapeutic case series.


Assuntos
Artroscopia/efeitos adversos , Impacto Femoroacetabular/cirurgia , Adolescente , Adulto , Idoso , Feminino , Humanos , Curva de Aprendizado , Masculino , Pessoa de Meia-Idade , Satisfação do Paciente , Complicações Pós-Operatórias/classificação , Estudos Retrospectivos , Adulto Jovem
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