RESUMO
Suppression of resistance in acute myeloid leukemia cells to TRAIL-induced apoptosis in multicellular aggregates, was studied using small molecule inhibitors of the activation of the transcription factor NF-kB - NF-k9 Activation Inhibitor IV and JSH-23 at non-toxic concentrations. NF-kB Activation Inhibitor IV and JSH-23 reduced resistance in the acute myeloid leukemia cells in multicellular aggregates to cytotoxic action of recombinant protein izTRAIL. It is shown that the use of these inhibitors decreased the phosphorylation of the RelA (p65) as a main marker activation of the transcription factor NF-kB. We discuss a possible reason for increasing resistance in acute myeloid leukemia cells to TRAIL-induced apoptosis in multicellular aggregates.
Assuntos
Leucemia Mieloide Aguda/tratamento farmacológico , NF-kappa B/antagonistas & inibidores , Ligante Indutor de Apoptose Relacionado a TNF/metabolismo , Fator de Transcrição RelA/metabolismo , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/genética , Humanos , Leucemia Mieloide Aguda/genética , Fenilenodiaminas/administração & dosagem , Fosforilação/efeitos dos fármacos , Agregados Proteicos/efeitos dos fármacos , Ligante Indutor de Apoptose Relacionado a TNF/administração & dosagem , Fator de Transcrição RelA/química , Ativação Transcricional/efeitos dos fármacosRESUMO
Data on the current radioecological status of lake Bolshoi Igish in the Middle Ural are presented in the paper. The lake is situated on the central axis of the East-Urals radioactive trace which was formed as a result of the 1957 nuclear accident at the Mayak Production Association. The distribution of 90Sr and 137Cs in the major components of the water basin is shown. Seasonal dynamics of the specific activity of radionuclides in water and in bottom deposits has been investigated. The current community of the higher water plant species has been studied. The paper discusses the concentration of 90Sr and 137Cs in the macrophyts of lake Bolshoi Igish depending on their species.