Chronic hyperglycemia and intracranial meningiomas.

Meningiomas are among the most common primary tumors of the central nervous system. Previous research into the meningioma histological appearance, genetic markers, transcriptome and epigenetic landscape has revealed that benign meningiomas significantly differ in their glucose metabolism compared to aggressive lesions. However, a correlation between the systemic glucose metabolism and the metabolism of the tumor hasn't yet been found. We hypothesized that chronic levels of glycaemia (approximated with glycated hemoglobin (HbA1c)) are different in patients with aggressive and benign meningiomas. The study encompassed 71 patients with de novo intracranial meningiomas, operated on in three European hospitals, two in Croatia and one in Spain. Our results show that patients with WHO grade 2 meningiomas had significantly higher HbA1c values compared to patients with grade 1 lesions (P = 0.0290). We also found a significant number of patients (19/71; 26.7%) being hyperglycemic, harboring all the risks that such a condition entails. Finally, we found a significant correlation between our patients' age and their preoperative HbA1c levels (P = 0.0008, ρ(rho) = 0.388), suggesting that older meningioma patients are at a higher risk of having their glycaemia severely dysregulated. These findings are especially important considering the current routine and wide-spread use of corticosteroids as anti-edematous treatment. Further research in this area could lead to better understanding of meningiomas and have immediate clinical impact.

Analytical potential of total reflection X-ray fluorescence spectrometry for simultaneous determination of iron, copper and zinc in human blood serum and plasma.

Due to many roles of trace elements such as Fe, Cu and Zn in various physiological and pathophysiological processes, their determination in serum and plasma is of high clinical relevance. In the present study, for the first time, the effect of serum and plasma preparation parameters (dilution factor and sample deposition volume) on the quality of results obtained by TXRF analysis was evaluated by means of experimental design tools (response surface analysis). It was found that the best strategy was the direct analysis of both human fluids without a previous dilution step. The accuracy and precision of the proposed methods were evaluated by analysis of reference materials (ClinChek® Plasma Control Level II and Seronorm™ Trace Elements Serum L-1). TXRF results agreed with the reference values and no significant differences at 95% confidence level were found. Limits of detection for the elements of interest were also adequate, taking into account their typical concentration ranges in real serum and plasma samples. Finally, the developed TXRF methods were applied to a set of serum and plasma samples from patients with different genders, ages and diagnoses, previously analysed by ICP-OES and ICP-MS techniques. The results showed good agreement between both analytical approaches. These results suggest that the proposed TXRF method provides reliable results thus being suitable for plasma and serum analysis, but in a simpler and more sustainable way.