Isolated limb perfusion with cytostatic drug leakage. / Perfusión aislada de extremidad inferior con fuga de quimioterapéutico.

Isolated limb perfusion is the treatment of stage III melanoma with in-transit metastasis. This technique allows the administration of cytostatics at an effective concentration and temperature, which could not be administered systemically because of their toxicity. The toxicity due to leakage of the chemotherapy agent from the limb into the systemic circulation is the most serious short-term complication, and is manifested by a systemic inflammatory response syndrome in the immediate post-intervention period. Early detection of this complication and its peri-operative management requires a multidisciplinary approach, in which the anaesthesiologist plays a key role. A case of isolated lower limb perfusion is reported in which the procedure had to be interrupted due to the passage of tumour necrosis factor into the systemic circulation, with severe intra-operative haemodynamic repercussions.

Seleccion biologica de Bordetella pertussis y Salmonella schottmuelleri empleando raton diabetico. / Biological selection of Bordetella pertussis and Sallmonella schottmuelleri using diabetic mices

Por inoculacion de ratones diabetizados con alizana y recuperacion de los microorganismos inoculados, se logro incrementar la virulencia de una cepa de Bordetella pertussis y se aumento la antigenicidad para reacciones de aglutinacion de una cepa de Salmonella schottmuelleri