α-Labeling of J591, an Antibody Targeting Prostate-Specific Membrane Antigen: The Technique and Considerations from the First Dedicated Production Lab at an Academic Institution in the United States.

The protein expression of the prostate-specific membrane antigen correlates with unfavorable or aggressive histologic features of prostate cancer, resulting in use as a diagnostic PET imaging radiotracer and therapeutic target. Here, we discuss the methods to develop 225Ac-DOTA-J591, an α-labeled compound targeting an extracellular epitope of prostate-specific membrane antigen, which is currently being studied in early clinical trials. In addition, we review quality control, radiation safety measures, and clinical considerations before administration of this radioimmunotherapeutic agent.

Radium-223 Dichloride in Peritoneal Dialysate Following Treatment of Metastatic Castration-resistant Prostate Cancer.

ABSTRACT: Radium-223 dichloride was administrated to a patient with castration-resistant metastatic prostate cancer (mCRPC) undergoing in-home peritoneal dialysis. The peritoneal dialysate and tubing sets were collected following four treatment cycles and analyzed for removable contamination, dose rate, and radioactivity. The purpose of the study was to assess radiation safety concerns and compare data with patients having normal renal function. Sixty-two liters of dialysate in 22 samples were collected over 4 mo. The mean surface dose rate of the dialysate bags was 0.16 µSv h-1 (range 0.11-0.23 µSv h-1). The highest measured removable surface activity was 150 dpm per 100 cm2. The mean cumulative percent of administered activity was 1.7% (range 1.4-1.9%). The mean concentration of 223Ra in dialysate was 4.0 kBq L-1 (range 0.56-14.1 kBq L-1). There was no association between the measured 223Ra activity in dialysate with peritoneal dwell time or the number of exchanges following administration. The measurement of the 211Bi and 223Ra ratio in the dialysate compared with a standard showed an increase of 23% by 40 h post administration. The data presented suggest that 223Ra dialysate can be safely managed in the home without risk of radiation dose or contamination if minimal precautions are taken. Patients with normal renal function have been shown to excrete up to six times more 223Ra in urine compared to those undergoing hemodialysis or peritoneal dialysis. A potential consequence may be an increase in 223Ra activity transiting GI tract leading to intestinal effects.