Forkhead Box Protein FOXK1 Disrupts the Circadian Rhythm to Promote Breast Tumorigenesis in Response to Insulin Resistance.

The dysregulation of circadian rhythm oscillation is a prominent feature of various solid tumors. Thus, clarifying the molecular mechanisms that maintain the circadian clock is important. In the present study, we revealed that the transcription factor forkhead box FOXK1 functions as an oncogene in breast cancer. We showed that FOXK1 recruits multiple transcription corepressor complexes, including NCoR/SMRT, SIN3A, NuRD, and REST/CoREST. Among them, the FOXK1/NCoR/SIN3A complex transcriptionally regulates a cohort of genes, including CLOCK, PER2, and CRY2, that are critically involved in the circadian rhythm. The complex promoted the proliferation of breast cancer cells by disturbing the circadian rhythm oscillation. Notably, the nuclear expression of FOXK1 was positively correlated with tumor grade. Insulin resistance gradually became more severe with tumor progression and was accompanied by the increased expression of OGT, which caused the nuclear translocation and increased expression of FOXK1. Additionally, we found that metformin downregulates FOXK1 and exports it from the nucleus, while HDAC inhibitors (HDACi) inhibit the FOXK1-related enzymatic activity. Combined treatment enhanced the expression of circadian clock genes through the regulation of FOXK1, thereby exerting an antitumor effect, indicating that highly nuclear FOXK1-expressing breast cancers are potential candidates for the combined application of metformin and HDACi.

Long-term evaluating the strengthening effects of iron-carbon mediator for coking wastewater treatment in EGSB reactor.

Coking wastewater (CWW) treatment is difficult due to its complex composition and high biological toxicity. Iron-carbon mediators was used to enhance the treatment of CWW through iron-carbon microelectrolysis (ICME). The results indicated that the removal rate of COD and phenolic compounds were enhanced by 24.1 % and 23.5 %, while biogas production and methane content were promoted by 50 % and 7 %. Microbial community analysis indicated that iron-carbon mediators had a transformative impact on the reactor's performance and dependability by enriching microorganisms involved in direct and indirect electron transfer, such as Anaerolineae and Methanothrix. The mediator also produced noteworthy gains in LB-EPS and TB-EPS, increasing by roughly 109.3 % and 211.6 %, respectively. PICRISt analysis demonstrated that iron-carbon mediators effectively augment the abundance of functional genes associated with metabolism, Citrate cycle, and EET pathway. This study provides a new approach for CWW treatment.

Diverse drug delivery systems for the enhancement of cancer immunotherapy: an overview.

Despite the clear benefits demonstrated by immunotherapy, there is still an inevitable off-target effect resulting in serious adverse immune reactions. In recent years, the research and development of Drug Delivery System (DDS) has received increased prominence. In decades of development, DDS has demonstrated the ability to deliver drugs in a precisely targeted manner to mitigate side effects and has the advantages of flexible control of drug release, improved pharmacokinetics, and drug distribution. Therefore, we consider that combining cancer immunotherapy with DDS can enhance the anti-tumor ability. In this paper, we provide an overview of the latest drug delivery strategies in cancer immunotherapy and briefly introduce the characteristics of DDS based on nano-carriers (liposomes, polymer nano-micelles, mesoporous silica, extracellular vesicles, etc.) and coupling technology (ADCs, PDCs and targeted protein degradation). Our aim is to show readers a variety of drug delivery platforms under different immune mechanisms, and analyze their advantages and limitations, to provide more superior and accurate targeting strategies for cancer immunotherapy.

FOXK1 promotes hormonally responsive breast carcinogenesis by suppressing apoptosis.

BACKGROUND: Globally, breast cancer constitutes the predominant malignancy in women. Abnormal regulation of epigenetic factors plays a key role in the development of tumors. Anti-apoptosis is a characteristic of tumor cells. Therefore, exploring and identifying relevant epigenetic factors that regulate the apoptosis of tumor cells is the foundation for clarifying the pathogenesis of tumors and achieving precision antitumor therapy. METHOD: This study focused on exploring the epigenetic mechanism of FOXK1 in the development of estrogen receptor-positive (ER+ ) breast cancer. We used overexpressing FLAG-FOXK1 MCF-7 cells to perform silver staining mass spectrometry analysis and conducted Co-IP experiments to verify the interactions. ChIP-seq was conducted on MCF-7 cells to examine FOXK1's binding across the genome and its transcriptional target sites. To validate the ChIP-seq results, qChIP, western blotting, and quantitative polymerase chain reaction (qPCR) were performed. Through TUNEL assay, cell counting assay, colony formation assay, and the mouse xenograft models, the effect of FOXK1 on breast cancer progression was detected. Finally, by analyzing online databases, the correlation between FOXK1 and the survival of breast cancer patients was examined. RESULTS: FOXK1 interacts with the REST/CoREST transcriptional corepression complex to transcriptionally inhibit target genes representing the apoptotic pathway. Abnormally high expression of FOXK1 prevents the apoptosis of ER+ breast cancer cells in vitro and promotes ER+ breast tumor progression in vivo. Furthermore, the expression of FOXK1 is negatively correlated with the survival of ER+ breast cancer patients. CONCLUSION: FOXK1 promotes ER+ breast carcinogenesis through anti-apoptosis and acts as a potential target for ER+ breast cancer treatment.

Zinc-finger protein CXXC5 promotes breast carcinogenesis by regulating the TSC1/mTOR signaling pathway.

CXXC5, a member of the CXXC family of zinc-finger proteins, is associated with numerous pathological processes. However, the pathophysiological function of CXXC5 has not been clearly established. Herein, we found that CXXC5 interacts with the CRL4B and NuRD complexes. Screening of transcriptional targets downstream of the CXXC5-CRL4B-NuRD complex by next-generation sequencing (chromatin immunoprecipitation sequencing) revealed that the complex regulates the transcriptional repression process of a cohort of genes, including TSC1 (tuberous sclerosis complex subunit 1), which play important roles in cell growth and mammalian target of rapamycin signaling pathway regulation, and whose abnormal regulation results in the activation of programmed cell death-ligand protein 1 (PD-L1). Intriguingly, CXXC5 expression increased after stimulation with vitamin B2 but decreased after vitamin D treatment. We also found that the CXXC5-CRL4B-NuRD complex promotes the proliferation of tumor cells in vitro and accelerates the growth of breast cancer in vivo. The expression of CXXC5, CUL4B, and MTA1 increased during the occurrence and development of breast cancer, and correspondingly, TSC1 expression decreased. Meanwhile, a high expression of CXXC5 was positively correlated with the histological grade of high malignancy and poor survival of patients. In conclusion, our study revealed that CXXC5-mediated TSC1 suppression activates the mammalian target of rapamycin pathway, reduces autophagic cell death, induces PD-L1-mediated immune suppression, and results in tumor development, shedding light on the mechanism of the pathophysiological function of CXXC5.

Eruptive syringomas: Summary of ninety cases and a brief literature review.

BACKGROUND: Eruptive syringomas is a rare variant of syringoma, which is a benign adenoma differentiated from the terminal ducts of the eccrine glands. Nowadays, it's widely valued because of obvious skin lesions, large scope of influence, and high misdiagnosis rate. OBJECTIVES: We aim to explore the clinical features of eruptive syringomas and the current research progress. MATERIALS AND METHODS: We firstly summarized the clinical features of 90 cases of eruptive syringomas. Then, the chi-square test was used to analyze the relationship between the onset site of eruptive syringomas and age, as well as gender. Finally, we briefly reviewed the previous literature. RESULTS: During 12 years, 90 cases of eruptive syringomas were diagnosed in our hospital, including 28 males (31.1%) and 62 females (68.9%). The average diagnosed age was 28.8. Patients from 20 to 40 years old is 63 (70%), which is the most. 60 (66.7%) patients had the course for more than 1 year. Among onset sites, the neck, chest, and abdomen were in the top three. The chi-square test showed that there were no significant differences in the onset sites of patients aged ≤ 20 and >20 years old (p-value = 0.181), as well as male and female (p-value = 0.363). CONCLUSION: We found that more female than male was affected, and the most common onset sites were the neck, chest, and abdomen. Neither age nor gender was significantly associated with onset site distribution. Our study provides some data support for the research of eruptive syringomas.

Multilayered insights: a machine learning approach for personalized prognostic assessment in hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) is a complex malignancy, and precise prognosis assessment is vital for personalized treatment decisions. Objective: This study aimed to develop a multi-level prognostic risk model for HCC, offering individualized prognosis assessment and treatment guidance. Methods: By utilizing data from The Cancer Genome Atlas (TCGA) and the Surveillance, Epidemiology, and End Results (SEER) database, we performed differential gene expression analysis to identify genes associated with survival in HCC patients. The HCC Differential Gene Prognostic Model (HCC-DGPM) was developed through multivariate Cox regression. Clinical indicators were incorporated into the HCC-DGPM using Cox regression, leading to the creation of the HCC Multilevel Prognostic Model (HCC-MLPM). Immune function was evaluated using single-sample Gene Set Enrichment Analysis (ssGSEA), and immune cell infiltration was assessed. Patient responsiveness to immunotherapy was evaluated using the Immunophenoscore (IPS). Clinical drug responsiveness was investigated using drug-related information from the TCGA database. Cox regression, Kaplan-Meier analysis, and trend association tests were conducted. Results: Seven differentially expressed genes from the TCGA database were used to construct the HCC-DGPM. Additionally, four clinical indicators associated with survival were identified from the SEER database for model adjustment. The adjusted HCC-MLPM showed significantly improved discriminative capacity (AUC=0.819 vs. 0.724). External validation involving 153 HCC patients from the International Cancer Genome Consortium (ICGC) database verified the performance of the HCC-MLPM (AUC=0.776). Significantly, the HCC-MLPM exhibited predictive capacity for patient response to immunotherapy and clinical drug efficacy (P < 0.05). Conclusion: This study offers comprehensive insights into HCC prognosis and develops predictive models to enhance patient outcomes. The evaluation of immune function, immune cell infiltration, and clinical drug responsiveness enhances our comprehension and management of HCC.

Effect of hydrothermal pretreatment on the degrease performance and liquid substances transformation of kitchen waste.

Hydrothermal treatment (HT) is a pragmatic approach for pretreatment of kitchen waste (KW). This work investigated the effect of hydrothermal pretreatment (HTP) on the deoiling, desalting and liquid substances transformation of KW. The orthogonal test method was used to study the effects of three factors at five levels, including solid to liquid ratio (A1-5), heating time (B1-5) and hydrothermal temperature (C1-5). The results indicated that the floatable oil content was improved significantly after HTP. The highest floatable oil content was 84.54 mL/kg at the hydrothermal condition of 1/1.5, 20 min and 100 °C, which was 2.42 times higher than the control. The maximum desalination ratio (92.66%) was at A5B1C5 (1/2.5, 5 min, 100 °C), which was 4.48 times higher than control group (No.0) (20.67%). The VFAs concentration was the highest (11441.05 mg/kg) at 1/2.5, 5 min and 100 °C, which increased by 711.03% compared to the No.0 (1410.78 mg/kg). In addition, the maximum TOC value was obtained at 53530.84 mg/kg. After HTP, the acetic acid and butyric acid concentrations of the liquid phase increased, while the ethanol concentration decreased. The contents of T,NH4+-N and organic nitrogen in the liquid phase of the HTP system increased, while NO3--N remained at a low level (4.96-20.48 mg/kg). The range and variance analysis showed that the temperature had the greatest effect on the deoiling and the liquid substances transformation of KW among these three factors, followed by solid to liquid ratio and heating time. Based on the orthogonal experiment, the optimal parameters for KW deoiling were A3 (1/1.5), B4 (25 min) and C5 (100 °C). This work provided a reference for the KW deoiling and hence improve the efficient utilization of KW.

Coupling direct voltage and granular activated carbon modified nanoscale zero valent iron for enhancing anaerobic methane production.

Anaerobic digestion technology has been widely used because it has a unique advantage of producing biogas as a renewable energy source. Therefore, several methods were studied to facilitate anaerobic methane production process. Coupling direct voltage and single conductive particles was an effective method to improve anaerobic wastewater treatment efficiency and methane production. However, the enhancement method was limited in this process due to the current of direct voltage or the toxicity of nanoparticles. Therefore, the granular activated carbon loaded with nanoscale zero valent iron (GAC-NZVI) particles prepared by co-precipitation method were added to the anaerobic synthetic wastewater system with direct voltage (0.10 V) to improve the treatment efficiency in this study. GAC-NZVI particles were added into anaerobic system with 0.10 V direct voltage to enhance CH4 production process. The COD removal and total CH4 production were enhanced by 4.22 % and 10.83 % with GAC-NZVI particles. The measurement results of EPS and Fe concentration showed that GAC-NZVI particles promoted the secretion of EPS by microorganisms, which could improve the floc strength of granular sludge. The measurements of conductivity and cyclic voltammetry (CV) showed that particles accelerated the metabolism of microorganism and promoted the electron transfer process. The increasing of Methanothrix and Methanobacterium could strengthen the methanogenesis. The abundances of bacteria and archaea using indirect interspecies electron exchange form (such as H2 or formate transfer microorganisms) were decreased after adding the particles. The results indicated that anaerobic treatment efficiency could be enhanced under the combined action of direct voltage and particles.

Enhancing methane production of synthetic brewery water with granular activated carbon modified with nanoscale zero-valent iron (NZVI) in anaerobic system.

Anaerobic digestion is an effective treatment technology for wastewater. However, long HRT and low CH4 production limit the application of anaerobic treatment. Iron-based materials, carbon-based materials and Fe-C composite particles have been used in anaerobic processes. However, the strengthening effect of Fe-C composite particles on anaerobic systems requires further research. In this study, granular activated carbon (GAC) loaded with nanoscale zero-valent iron (NZVI) was prepared by a co-precipitation method and its morphology was characterized. Different concentrations of GAC-NZVI particles were used in the batch experiment to study the enhancing effect of the anaerobic biological treatment process. The water quality, sludge properties and microbial community were analyzed. The degradation rate of COD and total CH4 production increased by 9.38% and 14.29% with particles at a concentration of 1000 mg/L, respectively. The average methane yield was 169.86 mL CH4/g-COD removed, which was 9.39% higher than that of the control. The measurement results of extracellular polymeric substance (EPS), conductivity, cyclic voltammetry (CV) and Fe concentration indicated that the composite particles showed excellent electrical conductivity and promoted microorganism metabolism, which accelerated the use of substrates and methane generation. The 3-dimensional excitation (Ex) - emission (Em) matrix (3D-EEM) fluorescence spectroscopy of soluble microbial product (SMP) and EPS indicated that the particles could affect the endogenous respiration of microorganisms. Microbial community analysis revealed that the dominant genus Methanothrix (acetoclastic methanogens) increased by 13.32%, which could strengthen acetoclastic methanogenesis and lead to higher CH4 production. The abundance of hydrogenotrophic archaea decreased after the addition of GAC-NZVI. These results provide an alternate method for enhancing anaerobic wastewater treatment using conductive particles.

Distribution, inhalation and health risk of PM2.5 related PAHs in indoor environments.

To investigate the potential cancer risk resulting from exposure to air pollutants, polycyclic aromatic hydrocarbons (PAHs) bound to airborne particles (PM2.5) were assessed in one outdoor environment and four indoor environments before and during the Spring Festival of 2015. The average total PAH concentration was site-dependent, and the concentration decreased before and during the Spring Festival. Fluoranthene (Flt) was the most commonly occurring among the 16 priority PAHs, and benzo(a)pyrene (BaP) accounted for the largest portion of the total carcinogenic potency of PAHs in PM2.5. The average BaP levels, in both indoor and outdoor environments, considerably exceeded the maximum permissible risk level of 1 ng/m3. Hazard quotients were found to be much less than 1, indicating little risk in terms of non-carcinogenic effects. Carcinogenic health risks resulting from possible carcinogens were determined to be much less than 1.00E-06. According to the California and WHO reference protocol, using empty room data to estimate the carcinogenic health risk produced values that were 10% lower than those calculated using outdoor environmental data.

Bioaccessible trace metals in lip cosmetics and their health risks to female consumers.

Females can be exposed to toxic elements in lip cosmetics following ingestion. The bioaccessibility of Cr, Mn, Co, Ni, Cu, Cd, Sb and Pb in lip cosmetics (n = 32) were assessed via the dilute HCl extraction method, In Vitro Gastrointestinal protocol (IVG) and the United States Pharmacopeia Methodology (USPM), and then health risks were characterized. The total concentrations of trace metals (TMs) in lip cosmetics were in the range of 15.55-111.97 mg/kg (Mean: 60.99 mg/kg). Cu, Pb and Cr were the three major TMs and accounting for >75% of the total concentrations. Except Sb and Pb in 4/32 and 4/32 samples were higher than the US FDA (Food and Drug Administration of the United States) limits, the other TMs were lower than that limits. Only bioaccessible Pb in all samples significantly exceeded the FDA limit 0.1 mg/kg in candy. Using IVG or USPM might be preferable for evaluating the TMs exposure over HCl since they better represent gastrointestinal physiology. The estimated average daily intake (ADI) of bioaccessible ∑TMs through lip cosmetics ingestion of career women and female college students were under safety level. The long-term exposure of bioaccessible TMs by lip cosmetics using would inevitably cause non-carcinogenic health risk. This is the first report on the in vitro tests used for evaluating bioaccessible TMs in lip cosmetics.

Response of anaerobic granular sludge to iron oxide nanoparticles and multi-wall carbon nanotubes during beet sugar industrial wastewater treatment.

The accelerated use of iron oxide nanoparticles (IONPs) and multi-wall carbon nanotubes (MWCNTs) in the consumer and industrial sectors has triggered the need to understand their potential environmental impact. The response of anaerobic granular sludge (AGS) to IONPs and MWCNTs during the anaerobic digestion of beet sugar industrial wastewater (BSIW) was investigated in this study. The IONPs increased the biogas and subsequent CH4 production rates in comparison with MWCNTs and the control samples. This might be due to the utilization of IONPs and MWCNTs as conduits for electron transfer toward methanogens. The MWCNTs majorly enriched the bacterial growth, while IONP enrichment mostly benefitted the archaea population. Furthermore, scanning electron microscopy and confocal laser scanning microscopy revealed that AGS produced extracellular polymeric substances, which interacted with the IONPs and MWCNTs. This provided cell protection and prevented the nanoparticles from piercing through the membranes and thus cytotoxicity. The results provide useful information and insights on the adjustment of anaerobic microorganisms to the natural complex environment based on nanoparticles infiltration.

Exposure and health risk assessment of PM2.5-bound trace metals during winter in university campus in Northeast China.

In order to better understand the risk to students' health caused by pollution derived from fine particulate matter with an aerodynamic diameter <2.5mm (PM2.5), this study collected 189 samples in one outdoor and four different functional indoor environments of a research center in a university campus. Trace metals (TMs) bound to PM2.5 in outdoor and indoor environments were measured using X-ray fluorescence spectrometry. The TMs measured were: As, Co, Cd, Cr, Ni, Cu, Zn, Mn, Hg, and Pb. The measurements of PM2.5-bound TMs before and during the 2015 Spring Festival held in Northeast China were compared. Results showed that pollution due to PM2.5-bound TMs in outdoor and indoor environments was higher before than during the Spring Festival. Cu (in three indoor environments) and Zn (in an outdoor environment) showed the highest concentrations among the ten TMs that were measured. Hg showed the lowest concentrations in all the environments analyzed. The concentrations of PM2.5-bound TMs declined among four indoor environments in the following order: the atrium, the students' office (sampled just nine days before the Spring Festival), the laboratory, and an empty room. The potential carcinogenic and non-carcinogenic health risks derived from PM2.5-bound TMs were within safe limits for graduate and undergraduate students, according to the standards established by the United States Environmental Protection Agency (USEPA).

Influence of operating parameters on the fate and removal of three estrogens in a laboratory-scale AAO system.

A laboratory-scale anaerobic-anoxic-oxic (AAO) process was constructed to investigate the influence of hydraulic residence time (HRT) and sludge retention time (SRT) on the removal and fate of estrone (E1), 17ß-estradiol (E2) and 17α-ethinylestradiol (EE2), and their removal mechanisms in a biological treatment system. In an HRT range of 5-15 h, the highest removal efficiencies for E1, E2 and EE2 were obtained at an HRT of 8 h, with values of 91.2, 94.6 and 81.5%, respectively. When the SRT was increased from 10 to 20 d, all three estrogen removal efficiencies stayed above 80%, while the optimal SRT for each estrogen was different. The contribution of each tank for removal of the three estrogens was in the order of aerobic tank>anoxic tank>anaerobic tank. The optimal HRT and SRT for the removal of both the three estrogens and nutrients were 8 h and 15d, respectively. At this condition, respectively, about 50.7, 70.1 and 11.3% of E1, E2 and EE2 were biodegraded, 28.8, 17.2 and 50% were accumulated in the system, 8.3, 5.4 and 17.3% were discharged in the effluent, and 12.2, 7.3 and 20.34% were transported into excess sludge. It indicated that biodegradation by sludge microorganisms was the main removal mechanism of E1 and E2, while adsorption onto sludge was the main mechanism for EE2 removal.

Assessment on the occupational exposure of urban public bus drivers to bioaccessible trace metals through resuspended fraction of settled bus dust.

Limited information is available on the bioaccessible fraction of trace metals in the resuspended fraction of settled bus dust in order to estimate bus drivers' occupational exposure. In this study, 45 resuspended fraction of settled dust samples were collected from gasoline and compressed natural gas (CNG) powered buses and analyzed for trace metals and their fraction concentrations using a three-step sequential extraction procedure. Experimental results showed that zinc (Zn) had the greatest bioaccessible fraction, recorded as an average of 608.53 mg/kg, followed in order of decreasing concentration by 129.80 mg/kg lead (Pb), 56.77 mg/kg copper (Cu), 34.03 mg/kg chromium (Cr), 22.05 mg/kg nickel (Ni), 13.17 mg/kg arsenic (As) and 2.77 mg/kg cadmium (Cd). Among the three settled bus dust exposure pathways, ingestion was the main route. Total exposure hazard index (HIt) for non-carcinogenic effect trace metals was lower than the safety level of 1. The incremental lifetime cancer risk (ILCR) for drivers was estimated for trace metal exposure. Pb and Ni presented relatively high potential risks in the non-carcinogenic and potentially carcinogenic health assessment for all drivers. ILCR was in the range of 1.84E-05 to 7.37E-05 and 1.74E-05 to 6.95E-05 for gasoline and CNG buses, respectively.

Occurrence and removal efficiencies of eight EDCs and estrogenicity in a STP.

The occurrence and removal of eight endocrine disrupting compounds (EDCs), including estrone (E(1)), 17ß-estradiol (E(2)), estriol (E(3)), 17α-ethinylestradiol (EE(2)), diethylstilbestrol (DES), bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP), and their estrogenicities were investigated in a sewage treatment plant in Harbin city, China. The EDCs were extracted from wastewater samples by solid phase extraction (SPE) method and analyzed with gas chromatography coupled with mass spectrometry (GC-MS). The average concentrations in the influents and effluents ranged from 6.3 (EE(2)) to 1725.8 ng L(-1) (NP) and from

Kinetic degradation model and estrogenicity changes of EE2 (17alpha-ethinylestradiol) in aqueous solution by UV and UV/H2O2 technology.

The photochemical degradation and estrogenicity removal of 17alpha-ethinylestradiol in aqueous solutions was investigated via ultraviolet (UV) photolysis and UV/H(2)O(2) process with a low-pressure UV lamp. The results indicated that the kinetics of both oxidation processes well fitted the pseudo-first-order kinetics. EE(2) can be partially removed by UV radiation alone with kinetic constants increasing from 0.0054 to 0.2753 min(-1) with the UV intensity increase. The EE(2) degradation rate enhanced from 0.0364 to 0.0684 min(-1) when H(2)O(2) was combined with the photolysis process (UV/H(2)O(2)), even though EE(2) was not oxidized when same amounts of H(2)O(2) existed in the aqueous solutions. The kinetic parameters of pseudo-first-order kinetics showed positive correlation to UV intensity and also H(2)O(2) concentration, however negative to the initial EE(2) concentration. A regression model was developed for pseudo-first-order rate constant as a function of the UV intensity, H(2)O(2) concentration and initial EE(2) concentration, which could be used to estimate the EE(2) degradation rate at various operational conditions. The yeast estrogen screen (YES) was employed to evaluate the estrogenicity of photolytic water samples. Results showed that more than 95% of the estrogenicity was removed after 40 min irradiation and the parent compound EE(2) was mainly responsible for the observed estrogenicity.

Electrochemical degradation of 17-alpha-ethinylestradiol (EE2) and estrogenic activity changes.

17-alpha-ethinylestradiol (EE(2)) as one of typical representatives of steroid estrogens is detrimental to endocrine systems of animals and humans due to its potential estrogenic activity. The degradation of EE(2) in aquatic solution by electrocatalytic oxidation applying Ti-based SnO(2) (Ti/SnO(2)) electrode as the anode was investigated. A pseudo-first-order kinetics under various initial concentrations of EE(2) was obtained and it was found that the calculated kinetic constant was determined as 0.112 min(-1), 0.053 min(-1), 0.023 min(-1) and 0.019 min(-1), which tended to rise in inverse proportion to EE(2) concentration within selected content of EE(2) as 0.5 mg L(-1), 2.0 mg L(-1), 5 mg L(-1) and 10 mg L(-1). Frozen recombinant yeast was introduced to evaluate estrogenic activities of an aqueous solution of 2.0 mg L(-1) EE(2) and oxidation products. The concentration of EE(2) and EE(2) equivalents (EEEQs) decayed 96.5%, 96.3% respectively in 15 min and 78.5% reduction of TOC in 8 h was observed under current density conditions of 10 mA cm(-2) and 0.2 M Na(2)SO(4) as the electrolyte in the process of electrolysis. On the basis of the results obtained, it indicates that the estrogenic activities of the intermediate products can be negligible.