Mechanisms of the novel pesticide sodium dodecyl benzene sulfonate in the mitigation of protozoan ciliated pathogens during microalgal cultivation.

Protozoan ciliates represent a common biological contaminant during microalgae cultivation, which will lead to a decline in microalgae productivity. This study investigated the effectiveness of sodium dodecyl benzene sulfonate (SDBS) in controlling ciliate populations within microalgae cultures. SDBS concentrations of 160 mg/L and 100 mg/L were found to effectively manage the representative species of ciliates contamination by Euplotes vannus and Uronema marinum during the cultivation of Synechococcus and Chlorella, and the growth vitality of microalgae has been restored. Additionally, SDBS at these concentrations reduced oxidative stress resistance and induced membrane damage to remove biological pollutants by modulating enzyme activity, affecting lipid, energy, amino acid metabolism pathways, and processes such as translation and protein folding. This research provides insights into the mechanisms through which SDBS effectively combats protozoan ciliates during the microalgal cultivation. This contributes to reduce biological pollution, ensure the overall productivity and healthy and sustainable management of microalgae ecosystems.

Physiological and transcriptomic responses of seawater halobios to micro/nano-scale polystyrene-cadmium exposure in a marine food web.

Micro/nano-plastics (MPs/NPs) represent an emerging contaminant, posing a significant threat to oceanic halobios. While the adverse effects of joint pollutants on marine organisms are well-documented, the potential biological impacts on the food chain transmission resulting from combinations of MPs/NPs and heavy metals (HMs) remain largely unexplored. This study exposed the microbial loop to combined contaminants (MPs/NPs + HMs) for 48h, bacteria and contaminants are washed away before feeding to the traditional food chain, employing microscopic observation, biochemical detection, and transcriptome analysis to elucidate the toxicological mechanisms of the top predator. The findings revealed that MPs/NPs combined with Cd2+ could traverse both the microbial loop and classical food chain. Acute exposure significantly affected the carbon biomass of the top predator Tigriopus japonicus (75.8% lower). Elevated antioxidant enzyme activity led to lipid peroxidation, manifesting in increased malondialdehyde levels. Transcriptome sequencing showed substantial differential gene expression levels in T. japonicus under various treatments. The upregulation of genes associated with apoptosis and inflammatory responses, highlighting the impact of co-exposure on oxidative damage and necroptosis within cells. Notably, NPs-Cd exhibited stronger toxicity than MPs-Cd. NPs-Cd led to a greater decrease in the biomass of top predators, accompanied by lower activities of GSH, SOD, CAT, and GSH-PX, resulting in increased production of lipid peroxidation product MDA and higher oxidative stress levels. This investigation provides novel insights into the potential threats of MPs/NPs combined with Cd2+ on the microbial loop across traditional food chain, contributing to a more comprehensive assessment of the ecological risks associated with micro/nano-plastics and heavy metals.

The transformation of dissolved organic matter and formation of halogenated by-products during electrochemical advanced oxidation pretreatment for shale gas produced water.

Electrochemical advanced oxidation processes (EAOPs) have shown great potential for the treatment of shale gas produced water (SGPW). In this study, we investigated the transformation of dissolved organic matter (DOM) during EAOPs of SGPW and the formation of toxic halogenated by-products at various current densities, using fluorescence spectroscopy and Fourier transform ion cyclotron resonance mass spectrometry. We found that the priority of DOM removal was terrestrial humic-like > microbial humic-like > protein-like substances. Non-Halogenated organic compounds (non-HOCs) and HOCs were predominantly CHO, and CHOCl/CHOBr compounds in EAOP-treated SGPW, respectively. As applied current density and treatment time increased, the production of oxyhalides increased, with chlorate > bromate > perchlorate. Meanwhile, most DOM was mineralized, resulting in residual products with higher modified aromaticity index (AImod) and nominal oxidation state of carbon (NOSC). The resistants had lower mass-to-charge ratio (m/z), AImod, NOSC, and double bond equivalent minus oxygen per carbon ((DBE-O)/C). The dominant reactions were the addition of tri-oxygen and deallyl. Bromine addition dominated the reactions of halogenating addition, while chlorine addition took second place. Furthermore, the acute toxicity of SGPW was positively correlated with inorganic halogenated by-products. This study contributes to the understanding and improvement of EAOPs for the treatment of SGPW.

Oxidation treatment of shale gas produced water: Molecular changes in dissolved organic matter composition and toxicity evaluation.

Produced water (PW) is the largest waste stream generated by hydraulic fracturing in an unconventional shale gas reservoir. Oxidation processes (OPs) are frequently used as advanced treatment method in highly complicated water matrix treatments. However, the degradation efficiency is the main focus of research, organic compounds and their toxicity have not been properly explored. Here, we obtained the characterization and transformation of dissolved organic matters of PW samples from the first shale gas field of China by two selected OPs using FT-ICR MS. CHO, CHON, CHOS, and CHONS heterocyclic compounds associated with lignins/CRAM-like, aliphatic/proteins, and carbohydrates compounds were the major organic compounds identified. Electrochemical Fe2+/HClO oxidation preferentially removed aromatic structures, unsaturated hydrocarbons, and tannin compounds with a double-bond equivalence (DBE) value below 7 to more saturated compounds. Nevertheless, Fe (VI) degradation manifested in CHOS compounds with low DBE values, especially single bond compounds. Oxygen- and Sulfur-containing substances, primarily O4-11, S1O3-S1O12, N1S1O4, and N2S1O10 classes, were the main recalcitrant components in OPs. The toxicity assessment showed that the free-radical-formed Fe2+/HClO oxidation could cause significant DNA damage. Therefore, the toxicity response byproducts need spcial attention when conducting OPs. Our results led to discussions on designing appropriate treatment strategies and the development of PW discharge or reuse standards.

Molecular-level variation of dissolved organic matter and microbial structure of produced water during its early storage in Fuling shale gas field, China.

Shale gas-produced water (PW), the waste fluid generated during gas production, contains a large number of organic contaminants and high salinity matrix. Previous studies generally focused on the end-of-pipe treatment of the PW and ignored the early collection process. In this study, the transformation of the molecular composition and microbial community structure of the PW in the transportation and storage process (i.e., from the gas-liquid separator to the storage tank) were investigated. As the PW was transported from the gas-liquid separator to the portable storage tank, the dissolved organic matter (DOM) showed greater saturation, less oxidation, and lower polarity. DOMs with high O/C and low H/C ratios (numbers of oxygen and hydrogen divided by numbers of carbon) were eliminated, which may be due to precipitation or adsorption by the solids suspended in the PW. The values of double-bond equivalent (DBE), DBE/C (DBE divided by the number of carbon), and aromatic index (AI) decreased, likely because of the microbial degradation of aromatic compounds. The PW in the gas-liquid separator presented a lower biodiversity than that in the storage tank. The microbial community in the storage tank showed the coexistence of anaerobes and aerobes. Genera related to biocorrosion and souring were detected in the two facilities, thus indicating the necessity of more efficient anticorrosion strategies. This study helps to enhance the understanding of the environmental behavior of PW during shale gas collection and provides a scientific reference for the design and formulation of efficient transportation and storage strategies to prevent and control the environmental risk of shale gas-derived PW.

Foliar application of selenium and zinc to alleviate wheat (Triticum aestivum L.) cadmium toxicity and uptake from cadmium-contaminated soil.

Due to the large area of agricultural soils contaminated by Cd worldwide, cost-effective and practical method for safety food production are necessary. The roles of micronutrient on reducing Cd accumulation in crops are recently introduced. In the current study, a pot-culture experiment in the greenhouse was conducted to study the foliar spraying of Se (Na2SeO4) and Zn (ZnSO4) on physiological and growth parameters, as well as Cd concentrations in wheat plants grown in Cd-contaminated soil. The foliar was sprayed with four concentration of Se and Zn (0, 10, 20, and 40 mg L-1) at different growth stage (tillering, elongating and heading) and whole wheat plants were collected after maturity. Both foliar spraying with Se and Zn significantly enhanced the photosynthesis, tissue biomass and antioxidant enzyme activity. Additionally, Se and Zn application can also increase Se and Zn concentrations in different plant tissues. Selenium and Zn decreased malondialdehyde (MDA) and Cd concentrations in wheat grains, hulks, leaves, stalks and root in a dose-additive manner. Overall, Se and Zn both efficiently enhanced the wheat growth and Se and Zn concentrations, and simultaneously decreased the Cd concentration in wheat plant. Compared with Zn, Se more efficiently improved wheat growth and reduced Cd concentration in the wheat in a Cd-contaminated soil. Present results suggest that use of foliar spraying, especially Se, could be a cost-effective strategy and could be recommended for remediation of light-or moderate-polluted soils contaminated by Cd.

Evaluation of zeolite-supported microscale zero-valent iron as a potential adsorbent for Cd2+ and Pb2+ removal in permeable reactive barriers.

A new composite adsorbent, zeolite-supported microscale zero-valent iron (Z-mZVI) was evaluated as a potential adsorbent for the removal of Cd2+ and Pb2+ from aqueous solution using batch and column experiments. Adsorption isotherms were well fitted by Langmuir model, and the maximum adsorption capacity was 63.14 mg/g for Cd2+ and 154.61 mg/g for Pb2+, respectively. Both adsorption processes followed the pseudo-second-order model which indicated that the rate-limiting step for different initial concentration was dominated by chemical adsorption process. The coexistence of Cd2+ and Pb2+ caused the reduction of Cd2+ removal efficiency, but not for Pb2+. Z-mZVI has a high removal capacity for Cd2+ and Pb2+ over a wide pH range (3.0-6.8) as well as in the presence of competitive Ca2+ or Mg2+ ions (<2 mmol/L). Moreover, Z-mZVI shows a high immobilization capacity for the adsorbed Cd2+ and Pb2+ products, even at the acid solution (pH = 3.95). Column experiment confirmed that Z-mZVI could simultaneously remove Cd2+ and Pb2+ from solution efficiently. Thomas model can simulate the equilibrium adsorption capacity of Cd2+ and Pb2+ of the Z-mZVI column well. This study demonstrates that Z-mZVI is an efficient and promising reactive material in permeable reactive barriers for Cd2+ and Pb2+ removal from aqueous solution.