SMS2, a Novel Allele of OsINV3, Regulates Grain Size in Rice.

Grain size has an important effect on rice yield. Although several key genes that regulate seed size have been reported in rice, their molecular mechanisms remain unclear. In this study, a rice small grain size 2 (sms2) mutant was identified, and MutMap resequencing analysis results showed that a 2 bp insertion in the second exon of the LOC_Os02g01590 gene resulted in a grain length and width lower than those of the wild-type Teqing (TQ). We found that SMS2 encoded vacuolar acid invertase, a novel allele of OsINV3, which regulates grain size. GO and KEGG enrichment analyses showed that SMS2 was involved in endoplasmic reticulum protein synthesis, cysteine and methionine metabolism, and propionic acid metabolism, thereby regulating grain size. An analysis of sugar content in young panicles showed that SMS2 reduced sucrose, fructose, and starch contents, thus regulating grain size. A haplotype analysis showed that Hap2 of SMS2 had a longer grain and was widely present in indica rice varieties. Our results provide a new theoretical basis for the molecular and physiological mechanisms by which SMS2 regulates grain size.

Effect of Rapid Hollow Cathode Plasma Nitriding Treatment on Corrosion Resistance and Friction Performance of AISI 304 Stainless Steel.

Low-temperature plasma nitriding of austenitic stainless steel can ensure that its corrosion resistance does not deteriorate, improving surface hardness and wear performance. Nevertheless, it requires a longer processing time. The hollow cathode discharge effect helps increase the plasma density quickly while radiatively heating the workpiece. This work is based on the hollow cathode discharge effect to perform a rapid nitriding strengthening treatment on AISI 304 stainless steels. The experiments were conducted at three different temperatures (450, 475, and 500 °C) for 1 h in an ammonia atmosphere. The samples were characterized using various techniques, including SEM, AFM, XPS, XRD, and micro-hardness measurement. Potentiodynamic polarization and electrochemical impedance spectroscopy methods were employed to assess the electrochemical behavior of the different samples in a 3.5% NaCl solution. The finding suggests that rapid hollow cathode plasma nitriding can enhance the hardness, wear resistance, and corrosion properties of AISI 304 stainless steel.

Percutaneous Endoscopic Lumbar Discectomy for Lumbar Disc Herniation Using an Endoscopic Staining: A Technical Note.

Symptomatic lumbar disc herniation (LDH) is widely treated using percutaneous endoscopic lumbar discectomy (PELD). In the present PELD surgery, performing decompression under endoscope still takes a long time to explore the rupture site of annulus fibrosus, resulting in prolonged operation time and over-invasion of the undegenerated annulus fibrosus. A wide range of intraoperative exploration also induces an iatrogenic injury of the normal annulus fibrosus, even aggravating intervertebral disc degeneration, which may lead to early postoperative recurrence in severe case. Hence, it is important to seek a precise decompression in PELD surgery. Under this kind of realization, more spinal surgeons possibly choose a disc staining before performing decompression. However, the classical disc staining technique still has its shortcomings. First of all, an appropriate dose of staining cannot be accurately mastered, even induces unqualified staining effect. Second, the duration of surgery and the times of fluoroscopy will be increased. Finally, what surgeons see under the endoscope is the staining result but not the staining process. Hence, this is accomplished more effectively by designing procedures that perform fully visible disc staining under spinal endoscope. There is no specific research to discuss the technique note of endoscopic staining in PELD surgery. We have come up with a new original technology of endoscopic staining with methylene blue injection in PELD for treatment of LDH.