A glycoside hydrolase 30 protein BpXynC of Bacillus paralicheniformis NMSW12 recognized as A MAMP triggers plant immunity response.

Bacillus spp. has been widely used as a biocontrol agent to control plant diseases. However, little is known about mechanisms of the protein MAMP secreted by Bacillus spp. Herein, our study reported a glycoside hydrolase family 30 (GH30) protein, BpXynC, produced by the biocontrol bacteria Bacillus paralicheniformis NMSW12, that can induce cell death in several plant species. The results revealed that the recombinant protein triggers cell death in Nicotiana benthamiana in a BAK1-dependent manner and elicits an early defense response, including ROS burst, activation of MAPK cascades, and upregulation of plant immunity marker genes. BpXynC was also found to be a glucuronoxylanase that exhibits hydrolysis activity on xlyan. Two mutants of BpXynC which lost the glucuronoxylanase activity still retained the elicitor activity. The qRT-PCR results of defense-related genes showed that BpXynC induces plant immunity responses via an SA-mediated pathway. BpXynC and its mutants could induce resistance in N. benthamiana against infection by Sclerotinia sclerotiorum and tobacco mosaic virus (TMV). Furthermore, BpXynC-treated tomato fruits exhibited strong resistance to the infection of Phytophthora capsica. Overall, our study revealed that GH30 protein BpXynC can induce plant immunity response as MAMP, which can be further applied as a biopesticide to control plant diseases.

Bifunctional magnetic nanoparticles for analysis of aldehyde metabolites in exhaled breath of lung cancer patients.

We report here the preparation of dual-functionalized magnetic nanoparticles, with the nanoparticles as extraction sorbents, a magnetic solid phase extraction method was developed and applied for the analysis of trace amount of aldehydes in human exhaled breath condensate. In the material, octyl-functionalized internal surface provided hydrophobic groups for extraction, non-ionic surfactant (Tween-20)-coated outer surface offered hydrophilic network structure to prevent the access of macromolecules, strong magnetic property of nanoparticles simplified the analytical procedure. The experimental results showed that the prepared nanoparticles exhibited good dispersibility in aqueous solution and excellent extraction efficiency toward aldehydes. Six aldehydes were derivatized with 2,4-dinitrophenylhydrazine and then the formed hydrazones were extracted by the nanoparticles and analyzed by high-performance liquid chromatography-photo diode array detector. Under the optimal conditions, the method provided low limits of detection (2.9-21.5 nmol L(-1)), satisfactory reproducibility (relative standard deviations, 2.9-13.1%) and acceptable recoveries (73.7-133.1%). The developed method was applied successfully to determine the aldehydes metabolites in the exhaled breath condensate samples of healthy people and lung cancer patients. The dual-functionalized material is suitable for biological sample analysis. The proposed method provides an alternative approach for quantification of aldehyde metabolites in complex biological samples.