IGF2BP1 promotes LPS-induced NFκB activation and pro-inflammatory cytokines production in human macrophages and monocytes.

BACKGROUND: Lipopolysaccharide (LPS)-induced macrophage/monocyte activation and pro-inflammatory cytokines production are important mediators for periodontitis progression. The current study tested the potential role of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) in the process. METHODS: THP-1 human macrophages and primary human peripheral blood mononuclear cells (PBMCs) were treated with LPS. mRNA and protein expression of IGF2BP1 were tested by qPCR and Western blotting assay. IGF2BP1 expression was altered by shRNAs or CRISPR/Cas-9 gene editing methods. LPS-induced cytokine production was tested by ELISA assay. Cytokine mRNA expression was tested by the quantitative real-time reverse transcriptase polymerase chain reaction (qPCR) assay. RESULTS: In THP-1 human macrophages and PBMCs, treatment with LPS induced mRNA and protein expression of IGF2BP1. IGF2BP1 silencing (by targeted shRNAs) or CRISPR/Cas-9 knockout largely inhibited LPS-induced production of multiple pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin (IL)-1ß and IL-6. Conversely, forced over-expression of IGF2BP1 facilitated LPS-induced pro-inflammatory cytokines production in THP-1 cells. For the mechanism study, we show that IGF2BP1 co-immunoprecipitated with p65-p52 nuclear factor kappa B (NFκB) complex in nuclei of LPS-treated THP-1 cells. Significantly, LPS-induced p65-p52 nuclear translocation and NFκB activation were inhibited by IGF2BP1 silencing or CRISPR/Cas-9 knockout. CONCLUSION: IGF2BP1 promotes LPS-induced NFκB signalling and transcriptional activation in human macrophages and monocytes.

Non-joint effusion is associated with osteoarthritis in temporomandibular joints with disk displacement.

PURPOSE: The aim of this study was to examine the possible association between different grades of joint effusion (JE) and osteoarthritis (OA) in temporomandibular joint (TMJ) anterior disk displacement without reduction (ADDwoR). MATERIAL AND METHODS: A sample of 101 female patients 20-40 years of age with unilateral TMJ ADDwoR were retrospectively reviewed. JE and OA were diagnosed with magnetic resonance imaging (MRI). JE was subdivided into three different grades: grade 0, no or minimal effusion; grade 1, moderate effusion; and grade 2, extensive effusion. Eight categories of degenerative changes were used for screening for the existence of OA. Cases with no less than one type of degenerative change were diagnosed as OA. RESULTS: In all, 71 patients (70.3%) were diagnosed as having OA in the joints with disk displacement. In the univariate analysis, the proportion of subjects with non-JE (grade 0) was higher in the OA group (p = 0.003), while the proportion of subjects with extensive effusion (grade 2) was lower in the OA group (p = 0.02). In the multivariate logistic regression analysis, non-JE was independently associated with the development of OA (odds ratio = 5.68, 95% confidence interval = 1.10-29.37, P = 0.04). CONCLUSION: The results suggested that non-JE was associated with OA in the joints with ADDwoR.

Chronological changes in the microstructure of bone during peri-implant healing: a microcomputed tomographic evaluation.

Our objectives were to examine the titanium-bone interfaces chronologically and to clarify the process of osseointegration using microcomputed tomography (microCT). The mandibular premolars of 3 dogs were extracted and 12 weeks later 2 Straumann dental implants were installed in each quadrant. The microstructural changes at the bone-implant interface at the first, fourth, and eighth weeks after installation were evaluated by microCT and a 3-dimensional image was constructed. The microstructural measurements at the bone-implant interface, including the trabecular number (Tb.N), bone volume fraction (BV/TV), structure model index (SMI), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp) and connectivity density (Conn.D), were measured. Experimental outcomes were analysed for correlations between time and microstructural variables of trabecular bone. Tb.N increased significantly during the eighth week compared with that during the first and fourth week (p=0.001, 0.002). BV/TV increased generally with time and there were significant differences (p=0.003) between each time group. While Tb.Sp decreased with time and changed significantly during the eighth week compared with that during the first week (p=0.021), differences in SMI, Tb.Th and Conn.D did not differ significantly according to time of implant. The significant increase in the Tb.N and BV/TV during the eighth week after insertion of implants indicates that the formation of new bone before the eighth week is the key to osseointegration.

Effect of sinus membrane perforation on the survival of implants placed in combination with osteotome sinus floor elevation.

This study was conducted to evaluate implant survival with regard to effects of sinus membrane perforation that occurred during osteotome sinus floor elevation (OSFE). The study group consisted of 42 patients with edentulous posterior maxilla. Implants placement combined with OSFE without a grafting material was performed. All patients underwent a radiographic examination before surgery, to reveal the periimplant bone formation immediately after implant placement and 6 to 33 months later. Sinus membrane perforations were detected in 6 patients, and 6 implants were inserted in 6 sinus sites. No infection occurred in all sites, and all implants succeeded in the observation follow-up period. There was a 100% survival rate of implant in perforated sinuses, the same as in intact sinuses. Within the limits of this study, we can conclude that perforation of the sinus membrane does not compromise the short-term survival of dental implants placed in combination with OSFE.

Magnetic resonance imaging assessment of temporomandibular joint soft tissue injuries of intracapsular condylar fracture.

We evaluated the soft tissue of the temporomandibular joint (TMJ) with magnetic resonance imaging (MRI) after intracapsular condylar fracture. Eighteen consecutive patients (19 TMJ) were diagnosed between 1 January 2010 and 30 October 2011. They were examined using bilateral sagittal and coronal MRI, which were obtained immediately after injury to assess the displacement of the disc, whether there was a tear in capsule or the retrodiscal tissue, and whether there was an effusion in the joint. On the affected side MRI showed disc displacement in 15 of 19, tears in the capsule in 9, and tears in the retrodiscal tissue in 16. All 19 had joint effusions. It also showed 2 joints with abnormalities on the unaffected side. We conclude that MRI is useful for diagnosis and for estimating the amount of damage to the TMJ, and is helpful in planning treatment.

Real time 3D simulation for nose surgery and automatic individual prosthesis design.

This paper presents an intuitive nose surgery planning and simulation system, using 3D laser scan image and lateral X-ray image, to provide high quality prediction of the postoperative appearance, and design the patient specific prosthesis model automatically. After initial registration, the internal surface of soft tissue at the nose region was generated by the statistical data for soft tissue thickness adapted by the individual thickness information from the X-ray image. Then, the sketch contour of the 3D scan data on the lateral X-ray image was modified manually or adjusted automatically according to some aesthetic statistical data, to drive the simulation in real time by the state-of-the-art Laplacian surface deformation method. When satisfied with the 3D postoperative appearance, the deformation was mapped to the internal surface of soft tissue, and the change before and after simulation was utilized to generate the patient specific prosthesis model automatically. The surgeons who used the system confirmed that this planning system is attractive and has potential for daily clinical practice.

[A finite element analysis system for two-demension morphological analysis of the soft tissue in orthognathic surgery].

OBJECTIVE: To evaluate a finite element method (FEM) for analysis of the cranial-facial morphology. METHODS: The two-dimensional finite element analysis system was established and used to analysis the lateral side morphology of the soft tissue by the change of each finite unit of the soft tissue in a X-ray cranial-facial lateral cepholometrics film. RESULTS: The finite element analysis system was showing very well in the figures and data made by the system. CONCLUSION: Finite element analysis system may be a good supplement of the traditional X-ray cephalometrics to the soft tissue of orthognatics.