RESUMO
Bronchial asthma poses a serious threat to human health. Previous studies have documented the role of long noncoding RNAs (lncRNAs) in asthma. However, the molecular mechanism underlying bronchial asthma remains unclear. The aim of the present study was to evaluate the role of the lncRNA Opainteracting protein 5 antisense RNA1 (OIP5AS1) in the house dust miteinduced inflammatory response in human bronchial epithelial cells. BEAS2B cells were treated with Dermatophagoides pteronyssinus peptidase 1 (Der p1) to establish an in vitro model of asthma. OIP5AS1 expression levels increased in BEAS2B cells following Der p1 treatment, while microRNA (miR)1433p was downregulated. Additionally, the levels of the proinflammatory factors tumor necrosis factorα, interleukin (IL)6 and IL8 were measured, and apoptosis was evaluated following OIP5 silencing. OIP5AS1 knockdown reduced the inflammatory response and apoptosis in BEAS2B cells. Furthermore, using dual luciferase reporter assays and cotransfection experiments, it was demonstrated that the function of OIP5AS1 was mediated by miR1433p. miR1433p overexpression attenuated the Der p1induced inflammatory response and apoptosis of BEAS2B cells by targeting high mobility group box 1 (HMGB1). In summary, OIP5AS1 exacerbated Der p1induced inflammation and apoptosis in BEAS2B cells by targeting miR1433p via HMGB1.