Clinical and pathological significance of proliferation index and p53 expression in gastric adenocarcinoma.

PURPOSE: The purpose of our work was to investigate the association between proliferative index [proIDX] and expression index p53 (p53IDX) with the clinical and pathological characteristics of gastric adenocarcinoma. METHODS: The biopsy material of 90 patients operated on for gastric cancer was routinely processed in paraffin and archived. After the histopathological report was made, two study groups were formed, the first group (n=45) comprised biopsies with intestinal carcinoma and the second (n=45) biopsies of diffuse gastric cancer. In both cases, the control group consisted of biopsies of surrounding non-tumor tissue The routine Hematoxylin-Eosin and immunohistochemical ABC method with anti-Ki67 and anti-p53 antibodies was applied at sections 3-5 µm thick. The expression of Ki67 and p53 was quantified stereometrically. For statistical analysis SPSS (19.0) was used. RESULTS: Significantly higher Ki67 expression was found in both types of adenocarcinoma compared to the control group, as well as significant association of proIDX with most of testing parameters. Expression of p53 was significantly higher in the intestinal type compared to the diffuse type and the control group and was significantly associated with age and histological grade. Diffuse type particulary showed, significant association of p53IDX with most of the histological parameters tested. CONCLUSION: Our results point a highly significant correlation of the Ki67 and p53 expression with indicators of gastric adenocarcinoma progression, which may help to identify patients with an aggressive gastric adenocarcinoma phenotype.

Interplay between expression of leptin receptors and mucin histochemical aberrations in colorectal adenocarcinoma.

BACKGROUND: There is no information on the effects of leptin receptors expression on mucin-histochemical alterations in human colorectal adenocarcinoma. AIM: Testing the correlation of leptin receptors expression with histochemical dysregulation of mucins in colorectal adenocarcinoma. PATIENTS AND METHODS: The study included 75 patients with colorectal adenocarcinoma who underwent surgical resection. Following a routine histopathological tissue analysis, 3-4 µm thick cuts were made onto resected tumors, which underwent a routine Hematoxylin-Eosin, histochemical Alcian Blue-Periodic Acid Schiff, pH 2.5, and High Iron Diamine-Alcian Blue, pH 2.5, methods for mucin differentiation and immunohistochemical Avidin-Biotin peroxidase complex method with anti-Ki67 and anti-leptin receptor antibodies. Following the quantification of results for the statistical analysis, the statistical software package SPSS for Windows (13.0) was used, and the tests for analyzing the significance of differences and correlation analysis - Spearman's rank correlation coefficient, were conducted. RESULTS: Increased expression of leptin receptors is with highly significant correlation coefficient associated with hypersecretion of sialomucins. Significant positive correlation coefficient exists between the leptin receptors expression against neutral-fucomucins secretion. With weak and negative, but a significant correlation coefficient, leptin receptors expression is associated to the sulfomucins generation. CONCLUSIONS: Increased expression of leptin receptors in colorectal adenocarcinoma is associated with mucin-histochemical abnormalities that are manifested by sialomucins hypersecretion and reduction, ultimately resulting in the absence of sulfomucins secretion.

Involvement of leptin receptors expression in proliferation and neoangiogenesis in colorectal carcinoma.

PURPOSE: This study tested whether there exists a correlation between leptin receptors (LEPR) expression with proliferation and neoangiogenesis in colorectal carcinoma. METHODS: Enrolled were 75 patients with colorectal carcinoma, who underwent surgical tumor resection. After routine histopathological preparation, sections 3-4 µm thick were prepared. Routine H&E and immunohistochemical ABC method with anti-LEPR, anti-Ki67 and anti-CD 105 antibodies were performed. RESULTS: Pronounced or moderate LEPR expression in colorectal carcinoma was found in 77.3% of the cases. Absence of expression of LEPR correlated with low rate of proliferation in 94.1% of the cases, while high proliferation rate showed 92% of the cases with pronounced LEPR expression. Low grade neoangiogenesis correlated with absence of LEPR expression in 88.2% of the cases. In 92% of the cases with pronounced LEPR expression, high rate of angiogenesis was observed. The LEPR expression correlated significantly (p<0.001) with proliferation index (proIDX) and neoangiogenesis index (mvdIDX). The corresponded correlation coefficients indicated considerable strength of association between variables (r=0.63 and r=0.66). CONCLUSION: Our results demonstrated that LEPR expression in colorectal carcinoma significantly corresponded to proliferation index of tumor cells and neoangiogenesis, which could have significant therapeutic and prognostic implications.