Clinical significance of raf-1 kinase inhibitor protein in oral squamous cell carcinoma and its role in cell metastasis.

OBJECTIVE: To detect the expression pattern of raf-1 kinase inhibitor protein (RKIP) in oral squamous cell carcinoma (OSCC) samples and to explore its clinical significance in OSCC metastasis. PATIENTS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot assay were conducted to detect the expression of RKIP in OSCC tissues and cells. The relationship between RKIP expression and OSCC clinicopathological characteristics was statistically analyzed. Transwell assay, wound healing assay, and Western blot were used to detect the influence of RKIP on the metastasis ability of OSCC cells. RESULTS: RKIP was significantly downregulated in OSCC samples. Low expression of RKIP predicted high incidence of metastasis in OSCC patients. In vitro experiments demonstrated that overexpression of RKIP could significantly inhibit invasion and migration abilities of OSCC cells. CONCLUSIONS: RKIP was a novel factor involved in OSCC progression, which was a potential biomarker and therapeutic target for the patients.

[Radioimmunological localization study using 131-iodine radioalbelled monoclone antibody ASC5 against human tongue squamous-cell carcinoma.].

Nude mice carrying human tongue squamous-cell carcinoma xenografts were studied in vivo.McAb ASC5 were purified from ascitic fluid obtained from BALB/c mice pristane-primed prior to being inocualted intraperitoneally with hybridoma cell,using with protein A sepharose CL-4B.Nude mice bearing xenografts were given i.v,injection of McAb labelled with 1311 and 1251.Mice were imaged daily with PHO gamma camera and sacrificed at interval after i.v.injection,weighted and counted.Gamma scanning indicated that there were a larger amount of activity detectable in tumor at 72h after i.v.injection 1311ASC5,then the tumor could be more clearly visualized at 96h and remained strongly position over the 7-day period,with the background activity decreasing.Tumor to non-tumor ratio was more than 2.9.It was secondary best of the antibody uptake at 72h,approximately 9.20+/-1.96%ID/g(mean+/-SD),which compared with isotypical antibody-paired normal mouse IgG,the difference was significant at P<0.05.Results demonstrated that ASC5 is useful in localization diagnosis and management of cancer.