Phased, chromosome-scale genome assemblies of tetraploid potato reveal a complex genome, transcriptome, and predicted proteome landscape underpinning genetic diversity.

Cultivated potato is a clonally propagated autotetraploid species with a highly heterogeneous genome. Phased assemblies of six cultivars including two chromosome-scale phased genome assemblies revealed extensive allelic diversity, including altered coding and transcript sequences, preferential allele expression, and structural variation that collectively result in a highly complex transcriptome and predicted proteome, which are distributed across the homologous chromosomes. Wild species contribute to the extensive allelic diversity in tetraploid cultivars, demonstrating ancestral introgressions predating modern breeding efforts. As a clonally propagated autotetraploid that undergoes limited meiosis, dysfunctional and deleterious alleles are not purged in tetraploid potato. Nearly a quarter of the loci bore mutations are predicted to have a high negative impact on protein function, complicating breeder's efforts to reduce genetic load. The StCDF1 locus controls maturity, and analysis of six tetraploid genomes revealed that 12 allelic variants of StCDF1 are correlated with maturity in a dosage-dependent manner. Knowledge of the complexity of the tetraploid potato genome with its rampant structural variation and embedded deleterious and dysfunctional alleles will be key not only to implementing precision breeding of tetraploid cultivars but also to the construction of homozygous, diploid potato germplasm containing favorable alleles to capitalize on heterosis in F1 hybrids.

The use of DNA microarrays for the developmental expression analysis of cDNAs from the oesophageal gland cell region of Heterodera glycines.

Summary A microarray was printed containing cDNAs from a library made from cytoplasm microaspirated from the oesophageal gland cell region of parasitic stages of the soybean cyst nematode, Heterodera glycines. The array contained both previously described clones (Wang et al. Mol. Plant-Microbe Interact. 2001, 14, 536-544) and uncharacterized cDNAs. Fluorescent probes for array hybridization were prepared using RNA polymerase amplification of nematode mRNA. Developmental expression profiles of the arrayed cDNAs were determined by hybridizing the microarray with probes from parasitic and non-parasitic H. glycines life stages. Distinct patterns of developmental expression were ascertained for the previously described gland expressed genes. In addition, four H. glycines cDNAs (SCN1018, SCN1020, SCN1028 and SCN1167) were identified that showed up-regulation in one or more parasitic life stages. Clone SCN1018 encodes a C-type lectin domain and is expressed in the hypodermis of females. Clone SCN1020 encodes a probable S-adenosylmethionine synthetase. Clone SCN1028 encodes a piwi protein with high similarity to the germ-line-specific protein R06C7.1 of Caenorhabditis elegans. The sequence of SCN1167 had no similarity to known genes. This paper describes the first use of cDNA microarrays to analyse genes of a plant-parasitic nematode and establishes a functional method to mine nematode cDNA libraries.