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1.
J Steroid Biochem Mol Biol ; 152: 1-7, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25869557

RESUMO

Liver X receptor is a ligand-activated transcription factor, which is mainly involved in cholesterol homeostasis, bile acid and triglycerides metabolism, and, as recently discovered, in the glucose metabolism by direct regulation of liver glucokinase. Its modulation by exogenous factors, such as drugs, industrial by-products, and chemicals is documented. Owing to the abundance of these synthetic molecules in the environment, and to the established target role of this receptor, a number of representative compounds of phthalate, organophosphate and fibrate classes were tested as ligands/modulators of human liver X receptor, using an integrated approach, combining an in silico molecular docking technique with an optical SPR biosensor binding study. The compounds of interest were predicted and proved to target the oxysterols-binding site of human LXRα with measurable binding kinetic constants and with affinities ranging between 4.3 × 10(-7) and 4.3 × 10(-8)M. Additionally, non-cytotoxic concentration of these chemicals induced relevant changes in the LXRα gene expression levels and other target genes (SREBP-1c and LGK) in human liver hepatocellular carcinoma cell line (HepG2), as demonstrated by q-RT-PCR.


Assuntos
Poluentes Ambientais/metabolismo , Ácidos Fíbricos/metabolismo , Organofosfatos/metabolismo , Receptores Nucleares Órfãos/metabolismo , Ácidos Ftálicos/metabolismo , Sítios de Ligação , Linhagem Celular Tumoral , Poluentes Ambientais/farmacologia , Ácidos Fíbricos/farmacologia , Células Hep G2 , Humanos , Receptores X do Fígado , Simulação de Acoplamento Molecular , Organofosfatos/farmacologia , Receptores Nucleares Órfãos/biossíntese , Receptores Nucleares Órfãos/genética , Ácidos Ftálicos/farmacologia , Ligação Proteica , RNA Mensageiro/biossíntese , Receptores de Esteroides/metabolismo , Proteína de Ligação a Elemento Regulador de Esterol 1/biossíntese , Proteína de Ligação a Elemento Regulador de Esterol 1/genética , Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo
3.
Clinics ; 66(3): 407-410, 2011. tab
Artigo em Inglês | LILACS | ID: lil-585948

RESUMO

OBJECTIVES: The aim of this prospective study was to compare changes in lipid metabolism and nutritional status after either 6 and 12 months of follow-up in subjects with lipodystrophy syndrome after traditional lifestyle therapy with or without fibric acid analogue intervention (bezafibrate and clofibrate). METHODS: Food intake, alterations in body composition and metabolic abnormalities were assessed in subjects with lipodystrophy syndrome at the beginning of the study. The nutritional status and metabolic alterations of the subjects were monitored, and the subjects received nutritional counseling each time they were seen. The subjects were monitored either two times over a period no longer than six months (Group A; n = 18) or three times over a period of at least 12 months (Group B; n = 35). All of the subjects underwent nutrition counseling that was based on behavior modification. The fibric acid analogue was only given to patients with serum triglyceride levels above 400 mg/dL. RESULTS: After six months of follow-up, Group A showed no alterations in the experimental parameters. After twelve months, there was a decrease in serum triglyceride levels (410.4 ± 235.5 vs. 307.7 ± 150.5 mg/dL, p< 0.05) and an increase in both HDLc levels (37.9±36.6 vs. 44.9 ±27.9 mg/dL, p,0.05) and lean mass (79.9 ± 7.8 vs. 80.3 ± 9.9 percent, p< 0.05) in Group B. CONCLUSION: After one year of follow-up (three sessions of nutritional and medical counseling), the metabolic parameters of the subjects with lipodystrophy improved after traditional lifestyle therapy with or without fibric acid analogue intervention.


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ácidos Fíbricos/metabolismo , Síndrome de Lipodistrofia Associada ao HIV/metabolismo , Estado Nutricional , Antropometria , Terapia Antirretroviral de Alta Atividade , HDL-Colesterol/metabolismo , Ingestão de Alimentos , Seguimentos , Síndrome de Lipodistrofia Associada ao HIV/terapia , Hiperlipidemias/terapia , Estilo de Vida , Estudos Prospectivos , Estatísticas não Paramétricas , Fatores de Tempo , Triglicerídeos/metabolismo
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