RESUMO
PROBLEM: To study the prevalence of HHV-6 in endometrial biopsies among women experiencing recurrent implantation failure (RIF) after IVF/ET compared with controls. METHOD OF STUDY: Thirty women experiencing RIF after IVF/ET and 10 fertile women participated in the study. All women had endometrial biopsies taken in the luteal phase of their menstrual cycle for an endometrial immune profile (EIP) and HHV-6 mRNA as well as lymphocyte and granulocyte populations. The prevalence of HHV-6 in endometrial biopsies was determined, and biopsies for positive and negative expression of HHV-6 were compared with the results of their EIP and lymphocyte and granulocyte populations. RESULTS: Thirty-seven percentage of women with a history of RIF and 0% of controls demonstrated the presence of HHV-6 in their endometrial biopsies. No associations were found when the results of the endometrial immune profile were compared with the presence or absence of HHV-6. Significant increase in neutrophil-specific CD16b mRNA was found in HHV-6-positive samples, and the levels of B cells-related CD19 mRNA were lower in biopsies from women with RIF in comparison with normal controls. CONCLUSION: HHV-6 infection is an important factor in RIF.
Assuntos
Aborto Habitual/virologia , Endométrio/virologia , Infertilidade Feminina/virologia , Infecções por Roseolovirus/epidemiologia , Aborto Habitual/imunologia , Antígenos CD19/metabolismo , Linfócitos B/imunologia , Linfócitos B/metabolismo , Linfócitos B/virologia , Biópsia/métodos , Endométrio/imunologia , Endométrio/metabolismo , Feminino , Fertilização in vitro/métodos , Granulócitos/imunologia , Granulócitos/metabolismo , Granulócitos/virologia , Herpesvirus Humano 6 , Humanos , Infertilidade Feminina/imunologia , Infertilidade Feminina/metabolismo , Linfócitos/imunologia , Linfócitos/metabolismo , Linfócitos/virologia , Ciclo Menstrual/imunologia , Ciclo Menstrual/metabolismo , Neutrófilos/imunologia , Neutrófilos/metabolismo , Neutrófilos/virologia , Prevalência , RNA Mensageiro/metabolismo , Receptores de IgG/metabolismo , Infecções por Roseolovirus/metabolismoRESUMO
The goal of this work was the evaluation of the frequency of human CMV infection among the women, whose pregnancy ended in miscarriage, detection of active forms of infection and treatment before pregnancy. Virological and sero-immunological techniques were used. A total of 116 women who had miscarriages before the 28 week of pregnancy were submitted to the CMV test. 109 women (94.0%) demonstrated positive results. 49 women (42.2%) had active form of the cytomegalovirus infection. 13 women (26.5%) had the recurrent form and 36 patients (73.5%) had the persistent form of CMV infection (stage of productive replication). All the women with active CMVI were treated before the next pregnancy. Immunomodulatory therapy for the treatment was used.