Histopathological Study of Breast Cancer at the Oran University Hospital Center on a Population from Western Algeria.

Breast cancer (BC) is the most common cancer among women worldwide and also in Algeria. Despite therapeutic progress, it remains a cancer with serious physical and psychological consequences. MATERIALS AND METHODS: The objective of this study was to perform a histological analysis on 244 patients from the western Algerian population who were receiving treatment at the Oran University Hospital. RESULTS AND DISCUSSION: The findings of our study indicates that women are more likely to be affected, with a predominance of tumors in the right breast (77%), we also found that (72%) of patients had a personal history risk of developing breast cancer. The majority of the cases that were examined were in the age range of 40 to 50. In addition, we noticed that unemployed and married women were the most affected, with (86% and 95%, respectively). Total of (63%) patients used contraceptives known to be endocrine disruptors and (68%) of cases were breast-feeding.Our results revealed that the most frequent histological type is the infiltrative ductal carcinoma (77%) followed by the ductal carcinoma in situ which is (13%), on the other hand the infiltrative lobular carcinoma presents a very low percentage (9%). The SBR II grade is the most dominant with (81%) followed by the SBRIII grade (18%) and no cases of SBR I grade were observed. CONCLUSION: Breast cancer is one of the most complex and difficult to control diseases, especially in developing countries. Breast cancer screening remains a powerful way of improving early detection and survival rate. The Findings of our study demonstrate that histopathological analysis provides an important prognostic information and, if the grading protocol is followed consistently, reproducible results can be obtained for a favorable prognosis and management of breast cancer to reduce the burden of the disease. KEY WORDS: Breast cancer, risk factor, histopathology, chemotherapy and radiotherapy.

Molecular characterization and haplotypes of hydatid cyst isolates collected from humans and ruminants in Setif Province (northeast of Algeria) based on mitochondrial cytochrome C oxidase subunit 1 (mt-CO1) gene sequences.

Cystic echinococcosis (CE) is a disease that can be transmitted from animals to humans, caused by the metacestode of Echinococcus granulosus. The disease has significant health and economic impacts worldwide, particularly in endemic areas. The study aimed to evaluate the prevalence of hydatid cysts in ruminants (cattle and sheep) (n = 2060) from the Setif Province of Algeria using microscopy. The results showed that hydatid cysts were detected in 9.6% (198/2060) of ruminants, with a higher prevalence in cattle (16.8%; 56/333) compared to sheep (8.2%; 142/1727). Molecular techniques were used to analyze a subset of animals consisting of 30 sheep and 4 cattle. Specifically, a fragment of the mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) gene was sequenced and compared to sequences from seven humans from the same region. The results indicated that all isolates were identified as E. granulosus sensu stricto. Haplotype analysis identified 19 E. granulosus s.s. haplotypes arranged like a star, with the dominant haplotype (Hap04) at the center. Hap04 has been assigned a total of 17 positives, including positives from sheep, cattle, and two humans. This study is noteworthy for being the first to use a molecular approach to human and ruminant echinococcosis in Setif, a significant breeding region in Algeria.

Initial characteristics of cystic fibrosis in Algeria: Description of 34 pediatric cases.

BACKGROUND: Cystic fibrosis (CF) is a rare disease in Algeria, and its prognosis is poor in developing countries. The clinical and demographic knowledge of Algerian pediatric patients diagnosed with CF is incomplete due to the nonexistence of a national medical registry. Hence, the present study is the first Algerian multicentre study on CF. METHODS: This retrospective study was conducted in western Algeria. Over 1 year, the study included all pediatric patients with a confirmed diagnosis of CF in the pediatric hospital of Oran. Patient characteristics, clinical manifestations, and the prescribed treatment were reported. RESULTS: Thirty-four children (16 boys and 18 girls) participated in this study. Only 15 were diagnosed before the age of 6 months. The sweat chloride test was positive in all patients. Respiratory manifestations were found in all patients, chronic diarrhoea in 29 of them, and growth retardation in 10. Moreover, 25 (73.5%) had low to low intermediate socioeconomic levels. After diagnosis, respiratory complications marked the evolution of the 34 patients, with bronchial congestion observed in 33 of them, while 10 (29.4%) patients presented severe bronchopneumonia and 4 (11.8%) were affected by asthma. Consequently,  three (8.8%) died at an average age of 9 years mainly because of respiratory failure. CONCLUSION: The prognosis of CF is poor in Algeria compared to other developed countries due to the longer diagnostic delay and limited therapeutic alternatives. This representative subset of Algerian pediatric patients with CF will serve as a reference for future studies on CF in Algeria.

Knowledge, awareness and practice regarding cystic echinococcosis in Algeria: A cross-sectional survey among the general population.

Cystic echinococcosis (CE) is a parasitic zoonotic disease with a widespread occurrence, particularly in developing countries. Known as a neglected disease, it is highly prevalent in Algeria with a significant public health concern and substantial financial losses. The objectives of this study was to explore knowledge and awareness and practice of the Algerian population regarding this disease. A cross-sectional online-based survey was conducted between July 15 and September 25, 2022 using a self-administered questionnaire targeting all Algerian people of more than 18 years of age. Out of the total respondents, 87.7% have ever heard of CE with livestock rearing connection (OR:2.550, CI 95% OR: 1.108-5.870) and educational level (OR:3.826, CI 95% OR: 1.599-9.156) as the main factors associated with the level of awareness. Regarding their knowledge, results showed dispersing notions about the disease. The majority (84.8%) were aware of the zonnotic nature of the disease while only 49.6% knew that CE is caused by parasitic disease. Also, 83.3% were knowledgeable of the role of contaminated offal in the contamination of dogs which are considered as the main source of contamination of humans (65.19%). Regarding their practices, even home slaughtering was common (92.96%), some positive attitudes were prevalent where most of the participants declared washing vegetable before consumption (97.41%) and washing their hands after touching a dog (91.11%). Also, 68.29% among the dog owners declared that deworming their pets. This study has revealed that despite the high level of awareness and some positive attitudes, the level of knowledge about CE among the Algerian public has shown some gaps related to the epidemiology of the disease. These results could be helpful for the awareness campaigns programmers in their missions through focusing their efforts on raising the level of knowledge for the items that have shown a lack of understand which will be of great importance in the strategy of CE elimination.

[Hydatid cyst sampling by FTA Card method for characterizing Echinococcus granulosus sensu lato in Algeria. Preliminary results]. / Prélèvement des kystes hydatiques par la méthode FTA Card pour la caractérisation moléculaire d'Echinococcus granulosus sensu lato en Algérie. Résultats préliminaires.

Introduction and objectives: Cystic echinococcosis is highly endemic in Algeria and constitutes a major socio-economic problem. Typing the species of the Echinococcus granulosus sensu lato complex circulating in cattle requires the use of a hydatid cyst sampling method adapted to difficult field conditions (high heat and humidity, long transport time). The FTA Card method currently constitutes an effective means of preserving biological samples before their molecular analysis. In the present study, the FTA Card method was used in the collection of hydatid cysts to identify the species of E. granulosus sensu lato circulating in ruminants (intermediate hosts) in eastern Algeria. Material and methods: A PCR was carried out for 41 samples of hydatid cysts taken from six slaughterhouses in eastern Algeria, targeting the cox1 mitochondrial gene. PCR products were visualized by electrophoresis in a 1% agarose gel. Results and conclusion: The results of the molecular analysis of all hydatid cyst samples confirmed the presence of E. granulosus sensu stricto in sheep, cattle and camels. The ubiquitous nature of the G1 genotype has been demonstrated. The use of FTA Card sampling is an efficient and simple method to obtain a biological sample in order to characterize the species of E. granulosus sensu lato in Algeria. The good preservation of the DNA in this matrix will make it easier to obtain new molecular data from difficult regions. The identification of the species of the E. granulosus sensu lato complex involved in the biological cycle is an essential prerequisite for the implementation of control measures, since different host species participate in their evolutionary cycle. The characterization of E. granulosus genotypes is essential to define an appropriate control strategy against cystic echinococcosis.

Bayesian spatio-temporal analysis of the incidence of lung cancer in the North West of Algeria, 2014-2020.

Lung cancer is the most common type of cancer worldwide. This study assessed the spatio-temporal variations of the incidence rate of lung cancer between 2014 and 2020 in Chlef, a province in the North West of Algeria. Case data recoded by municipality, sex and age were collected from the oncology department in a local hospital. A hierarchical Bayesian spatial model, adjusted by urbanization level, with zero inflated Poisson distribution was used to study the variation of lung cancer incidence. A total of 250 lung cancer cases were registered during the study period, with a crude incidence rate of 4.12 per 100,000 inhabitants. The results of the model showed that residents in urban municipalities had a significantly higher risk of lung cancer than those in rural municipalities: incidence ratio rate (IRR) = 2.83 (95% CI: 1.91 - 4.31) and 1.80 (95% CI: 1.02 - 3.16) for men and women, respectively. In addition, the estimated incidence rate by the model for both sexes in the Chlef province indicated that only three urban municipalities had a higher incidence rate of lung cancer than the average of the province. The results of our study suggest that the risk factors for lung cancer in the North West of Algeria were mainly related to the level of urbanization. Our findings provide important information to guide the health authorities in designing measures for the surveillance and control of lung cancer.

First study of genital HPV infection among women living with HIV recruited from May to September 2018 in Eastern Algeria.

BACKGROUND: Genital HPV infection is recognized worldwide as one of the most common, if not the most common, sexually transmitted infection. Several studies have shown a higher prevalence of HPV infection in women living with HIV (WLWH). We aimed to determine the prevalence of HPV, the circulating genotypes, and its association with risk factors among Algerian WLHIV. METHODS: Cervical specimens were collected from 100 WLHIV. The detection of HPV infection was performed by Roche Linear Array test. RESULTS: The overall prevalence of HPV infection was determined at 32%, all including high risk HPV (HR-HPV). We found a great diversity of HR-HPV genotypes, the most common individually were HPV52, HPV16, HPV18 and HPV58, The highest prevalence was found in groups of genotype 52, 25%. HPV16 and 18 were less common (16%). Cervical cytology was abnormal in 66% (81.3% in the HPV + population), with a predominance of inflammatory lesions (75% HPV +), The most important determining risk factor for HPV infection in this series was a low CD4 T cell count <200/mm3 found in 72% of the HPV positive participants. CONCLUSION: Our study is an initial database, to be completed by a multicenter study to determine the most frequent genotypes in Algerian WLHIV, in order to discuss the introduction of the vaccine against HPV in Algeria, especially among WLHIV.

Breast cancer knowledge and practices amongst women in Algeria.

PURPOSE: The present study aims at investigating how Algerian women feel about Breast Cancer (BC) and Breast Self-Examination (BSE) in terms of knowledge and attitude and to determine the factors associated with performing BSE and its reluctance. METHODS: We carried out a cross-sectional survey between October 14, 2021 and November 14, 2022, targeting Algerian females aged more than 18 years and living in the country using a self-administered questionnaire. RESULTS: A total of 436 participants were involved in this study among which 41.28% were aged between 21 and 30 years and 34.86% were aged between 31 and 40 years. The average level of correct responses with regard to knowledge about BC was estimated at 51.31%, whereas it was 32.93% concerning knowledge about BC risk factors. Family history was the less reported incriminating factor for developing BC among the asked women (7.34%). Concerning alarming signs of BC, the current study revealed that Algerian women were less knowledgeable about breast dimpling-puckering (44.27%), breast inward traction (50.23%), breast redness (54.13%), and nipple position change (54.13%). In terms of belief in the utility of BSE in the early detection of BC, almost all the participants (97.98%) were convinced of its utility, whereas 96.33% of them showed an interest in learning more about it. In what concerns the early screening tests, approximately, four-fifth of the participant (77.52%) heard about them, while 94.72% believed that its early detection could reduce the disease's severity and mortality. CONCLUSION: These findings revealed an insufficient level of knowledge concerning BC, especially in regard to its risk factors and some alarming signs as well as a lack of knowledge about BSE and other BC screening tests; hence the necessity of awareness campaigns about this disease that should target categories with the lowest level of knowledge.

Pathologically confirmed women's breast cancer: A descriptive study of Tunisian and Algerian series.

BACKGROUND: Breast cancer (BC) is the most frequent malignancy among women in Tunisia and Algeria. Clinical and pathological characteristics of this cancer among these populations are not widely reported. The aim of the study was to report clinical and pathological characteristics of women's BC in a Tunisian and Algerian series. METHODS: Pathologically confirmed 1089 BCs were gathered in the pathology departments of three Northern Tunisian hospitals: Tunis military, Charles Nicolle and Jendouba and in the pathology department of Alger Douera hospital between January 2015 and December 2020. Clinical and pathological findings of the two series: age, tumor size, histological type, grading according to Scarff-Bloom Richardson grading system, lymph node status at the time of diagnosis in axillary lymphadenectomy specimens and the immunohistochemical expression of estrogen and progesterone receptors (ER/PR), HER2 and Ki-67, were collected from the pathological reports. RESULTS: The median age at diagnosis was 50 and 48 years in Tunisian and Algerian series, respectively (p = 0.016). The diagnosis of BC was made on surgical specimens (lumpectomy or mastectomy) in 373/491 (76%) cases of the Tunisian series and in 225/598 (37.6%) cases of the Algerian one. Median tumor size was 2.8 cm and 2.5 cm in Algerian and Tunisian series, respectively (p = 0.252). Invasive BCs not otherwise specified was observed in 440/481 (91.5%) BCs in Tunisian series and in 519/586 (88.6%) BCs in Algerian series. Axillary lymph node positive tumors were observed in 64.6% and 58.8% of Tunisian and Algerian women, respectively (p = 0.926). BCs were ER positive in 311/385 (80.8%) and 486/571 (85.1%) cases and HER2 positive in 86/283 (30.4%) and 60/385 (15.6%) cases of Tunisian and Algerian series, respectively. CONCLUSIONS: In Tunisia and Algeria, BC has poor prognostic factors with large tumor sizes and high rates of lymph nodes involvement at diagnosis.

Poor Self-Rated Oral Health Status and Associated Factors Amongst Adults in Algeria.

BACKGROUND: Understanding the proportion and correlates of self-rated oral health (SROH) can assist in prioritising interventions. The aim of this study was to assess the prevalence of poor SROH and associated factors in a national community survey amongst adults in Algeria. METHODS: This World Health Organisation (WHO) STEPS cross-sectional survey in Algeria in 2016 and 2017 included 6989 people (18-69 years; median age, 37 years) selected by multistage cluster sampling. The assessment included questionnaire information, physical measures, and biochemistry tests. The measures included questions on SROH, oral conditions, oral health behaviour, general health behaviour, and measures of health status. RESULTS: The sample included 6989 people aged 18 to 69 years. Of the total sample, 17.1% had removable dentures, 35.5% had 0 to 19 natural teeth, 43.9% had dental pain in the past year, 40.5% had impaired oral health-related quality of life (OHRQoL), 26.9% cleaned their teeth 2 times/d or more, 79.6% used toothpaste, and 41.2% had visited a dentist in the past year. The prevalence of poor SROH was 37.3%. In the final logistic regression model, older age (45-69 years) (adjusted odds ratio [AOR], 1.34; 95% confidence interval [CI], 1.09-1.65), having removable dentures (AOR, 1.46; 95% CI, 1.14-1.87), dental pain (AOR, 2.16; 95% CI, 1.82-2.57), impaired OHRQoL (AOR, 2.69; 95% CI, 2.26-3.20), current smokeless tobacco use (AOR, 1.45; 95% CI, 1.12-1.89), and inadequate fruit and vegetable intake (AOR, 2.69; 95% CI, 2.26-3.20) increased the odds of poor SROH. Men (AOR, 0.76; 95% CI, 0.65-0.90), having 20 or more teeth (AOR, 0.35; 95% CI, 0.28-0.42), twice or more a day teeth cleaning (AOR, 0.72; 95% CI, 0.60-0.86), and using toothpaste (AOR, 0.67; 95% CI, 0.55-0.82) were protective against poor SROH. CONCLUSIONS: Adults in Algeria reported a high prevalence of poor SROH, and several associated factors (sociodemographic, oral conditions, oral and general health-compromising behaviour) were found that can guide in oral health promotion strategies in Algeria.

[Evaluation of thyroid cancer data completeness and quality at a population-based cancer registry, Algeria]. / Évaluation de l'exhaustivité et de la qualité des données du cancer de la thyroïde dans un registre du cancer dans la population algérienne.

BACKGROUND: Over the last three decades the incidence of thyroid cancer (TC) has increased in many regions of the world, however little is known about TC incidence and trends in Algeria. MATERIAL AND METHODS: Using data from the Oran cancer registry (OCR) we assessed TC incidence and trends in Oran for the period 1996-2013 with the historical data method. The incidence curves were unstable and did not show any clear trend. Therefore, we actively collected data on TC for the period 1996-2013 using the multisource approach and the independent case ascertainment method. RESULTS: Analysis of actively collected and validated data showed a significant increase in the incidence of TC. We compared the two databases to identify differences. There were 558 TC cases during the period 1996-2013 in the OCR, while our active data collection enabled us to find 1,391 TC cases during the same period. The completeness rate in the OCR was 40.1%. These differences were due to our approach that consisted in the inclusion of a greater number of health facilities and laboratories (44 versus 23 in the OCR), and the active data collection in the nuclear medicine facility of the University Hospital of Tlemcen that we undertook. CONCLUSIONS: The application of the recommendations of the International Agency for Research on Cancer (IARC) to enhance data completeness and quality, and an active collection of TC data in the nuclear medicine facility of the University Hospital of Tlemcen should make the OCR an essential tool for decision-making in public health and for directing health policy towards health priorities.

[Ocular surface disease in glaucoma patients: Expert opinion and management algorithm in Algeria]. / Atteinte de la surface oculaire chez les patients glaucomateux : avis d'experts et algorithme de prise en charge en Algérie.

INTRODUCTION: The main goal of treatment for any glaucoma patient is to reduce the intraocular pressure. Unfortunately, the long-term use of glaucoma medications causes deleterious effects on the ocular surface, leading to impaired quality of life and failed glaucoma surgery. In addition, adverse effects of medications are a barrier to adherence and compliance, and this directly affects efficacy. It is therefore essential to regularly assess the ocular surface of glaucoma patients in order to detect any signs of damage early and act accordingly. Although the TFOS DEWS 2 now offers a strategy for diagnosing ocular surface pathologies, some examinations are unfortunately not feasible in Algeria due to lack of time and/or knowledge on the part of some ophthalmologists or are simply inaccessible to others. METHODS: Algerian experts (members of the ACOS club) met for the purpose of proposing an algorithm for the management of Algerian glaucoma patients, based on the recommendations of the DEWS, their own practice and the results of a preliminary national survey of ophthalmologists. RESULTS: The proposed algorithm allows evaluation of the ocular surface of the glaucoma patient in current practice by any Algerian ophthalmologist. CONCLUSION: Management of glaucoma patients should include a systematic examination of the ocular surface. The choice of treatment should be determined by the ocular surface condition of the glaucoma patient.

Colorectal cancer in a region of western of Algeria: results of 581 cases in 5 years.

Aims: The objective of this work is to evaluate the epidemiological profile of colorectal cancers, histologically proven over a 5-year period (2012-2016) in the Tlemcen region. Methods: A retrospective study of 581 cases of colorectal cancer collected at the epidemiology department of the University Hospital Center (UHC) of Tlemcen between January 2012 and December 2016 was performed. Epidemiological data were processed using SPSS version 25 and Microsoft Excel 2010. Results: The epidemiological profile has shown that colorectal cancer in our region ranks 3rd in both sexes. There were 322 men (55.4%) affected compared to 259 women (44.6%) with a sex ratio of 1.2. A predominance of males is noted in 50-60 age group, while for the female sex, the dominance is between 60-70 years old. The mean age of CRC occurrence was 60±13 years, with an extremity ranging from16 to 90. A significantly higher rate was recorded for rectal cancer (43.7%) followed by sigmoid colon (5.7%). Variable rates were recorded during the 5 years with a peak in 2014 (27.9%). Conclusion: Regular analysis of these data, if supplemented with additional data on diagnostic modalities like circular RNA diagnostic, will contribute to the assessment of the impact over time of public policies on nationally organized CRC screening.

Ectoparasites of the common gundi (Ctenodactylus gundi Rothmann) from the Aures Region, Algeria.

Ctenodactylus gundi were captured in the southern region of Aures, Algeria from December 2015 through June 2017 to assess their ectoparasite diversity. 63.5% of the gundi had one or more ectoparasites, while 36.5% had none. Eight species of ectoparasites were identified. The most abundant taxa were species of Neotrombicula (61.5%), Trombicula (10.6%), and Caenopsylla mira (4.8%). The mean intensity for arachnids follows: Neotrombicula spp. (14.3), Trombicula spp. (10.6), and Rhipicephalus (Boophilus) annulatus (1.6). Among arachnids, mean intensity of males was greater than females in contrast to that of the flea C. mira, whose mean intensity for females was 4.8 compared to that of males with 2.8. C. mira and Xenopsylla cheopis have previously been reported on the gundi, while the mites Neotrombicula spp., Trombicula spp., Eulaelaps stabularis, O. bacoti, the tick Rh. (Boophilus) annulatus and the louse Polyplax serrata are reported here for the first time. Prevalence was influenced by host sex and was higher in males than females. The highest prevalence was recorded in species of Neotrombicula (39.2%) and Trombicula (20.3%). The abundance of these ectoparasites was directly influenced with climatic conditions specially with temperature (P=0.0002), and air humidity (P=0.0014).

Analysis of vascular endothelial growth factor (VEGF) insertion/deletion gene polymorphism and environmental risk factors in sample of Algerian population with neovascular age-related macular degeneration (nAMD) / Analyse du polymorphisme insertion/délétion du gène de facteur de croissance endothélial vasculaire (VEGF) et des facteurs de risque environnementaux dans un échantillon de la population algérienne atteinte de la dégénérescence maculaire liée à l'âge néovasculaire (DMLAn)

Background: Increasing evidence shows that genetic and environmental factors can influence neovascular age-related macular degeneration (nAMD) risk. The aim of this study was first to analyse the association of insertion/deletion polymorphism in VEGF gene and environmental factors with the risk of nAMD, and then to investigate whether these factors have an impact on the age of onset of nAMD in a sample of the Algerian population. Methods: Seventy two patients with nAMD and one hundred twenty-four controls were recruited; standardized questionnaire was used to collect information regarding underlying systemic diseases and important environmental factors. Genotyping of VEGF (I/D) SNP was conducted using PCR-based assay approach, and statistical analyses were conducted using IBM SPSS statistics 21. Results: A significant association was reported of age (p < 0.05), smoking (p = 0.02), alcohol (p < 0.01), hypertension (p = 0.04), hyperlipidaemia (p = 0.008) and thyroid disease (p = 0.03) with nAMD. Also, Thyroid disease may have a role in accelerating the development of nAMD in an earlier age in our sample (p < 0.001). No association was found between the VEGF ­ 2549 I/D genotype and the presence of nAMD (p = 0.27), neither with the age of onset of nAMD (p = 0.21). Conclusion: Our results suggest that age, smoking, alcohol, hypertension, hyperlipidaemia and thyroid diseases are possible risk factors that could increase the risk of nAMD in a sample of Algerian population. In addition, VEGF ­ 2549 I/D might not be associated with the risk of nAMD development. Finally, thyroid disease may accelerate the development of nAMD in an earlier age.

Introduction: Un nombre croissant de preuves montrent que les facteurs génétiques et environnementaux peuvent influencer le risque de la forme néovasculaire de la dégénérescence maculaire liée à l'âge (DMLAn). L'objectif de cette étude était d'abord d'analyser l'association du polymorphisme d'insertion/délétion dans le gène VEGF et des facteurs environnementaux avec le risque de la DMLAn, puis d'étudier si ces facteurs ont un impact sur l'âge d'apparition de cette maladie dans un échantillon de population algérienne. Méthodes: Soixante-douze patients atteints de la DMLA et 124 témoins ont été recrutés ; un questionnaire standardisé a été utilisé pour recueillir des informations concernant les maladies systémiques sous-jacentes et les facteurs environnementaux importants. Le génotypage du SNP VEGF (I/D) a été réalisé par une l'approche de PCR standard, et les analyses statistiques ont été réalisées à l'aide du logiciel IBM SPSS statistics 21. Résultats: Une association significative a été rapportée entre l'âge (p < 0,05), le tabagisme (p = 0,02), l'alcool (p < 0,01), l'hypertension (p = 0,04), l'hyperlipidémie (p = 0,008) et les maladies thyroïdiennes (p = 0,03) avec la DMLAn. Les maladies thyroïdiennes peuvent jouer un rôle dans l'accélération du développement de la DMLAn à un âge plus précoce dans notre échantillon (p < 0,001). Aucune association n'a été trouvée entre le génotype VEGF ­ 2549 I/D et la présence de la DMLA néovasculaire (p = 0,27), ni avec l'âge d'apparition de cette pathologie (p = 0,21). Conclusion: Nos résultats suggèrent que l'âge, le tabagisme, l'alcool, l'hypertension, l'hyperlipidémie et les maladies thyroïdiennes sont des facteurs de risque possibles qui pourraient augmenter le risque de la DMLA néovasculaire. De plus, le VEGF ­ 2549 I/D pourrait ne pas être associé au risque de développement de la DMLA. Enfin, les maladies thyroïdiennes pourraient accélérer le développement de la DMLAn à un âge plus précoce.

Molecular characterization of Echinococcus granulosus sensu lato genotypes in dromedary camels from extreme Sahara of Algeria based on analysis of nad2 and nad5 genetic markers.

Cystic echinococcosis is parasitic disease caused by the metacestodes belonging to the Echinococcus granulosus sensu lato (s.l.) species complex. Cystic echinococcosis is of considerable economic and public health importance. It is endemic in both livestock and humans in North African countries, including Algeria. The present study aimed to characterize E. granulosus s.l. genotypes in dromedary camels (Camelus dromedarius) from the extreme Sahara of Algeria, using recently developed mitochondrial genetic markers (NADH dehydrogenase subunit 2 and NADH dehydrogenase subunit 5) for reliable identification of different genotypes. A total of 75 Echinococcus cysts were collected from 49 dromedary camels, including 65 and 10 cysts from 45 and four camels originating from two slaughterhouses of Tindouf and Illizi provinces, respectively. E. granulosus sensu stricto (s.s.) G1 and G3 were identified in camels from both areas based on nad5 (649 bp) gene sequences, whereas E. granulosus s.l. G6 was identified in camels from Tindouf region based on concatenated nad5 and nad2 gene sequences (total 1336 bp). Identified samples clustered into 11 different haplotypes (ALG1-ALG11), including four haplotypes (ALG8-ALG11) for E. granulosus s.s. G1, one haplotype (ALG7) for E. granulosus s.s. G3, and six haplotypes (ALG1-ALG6) for E. granulosus s.l. G6. The present study provides valuable molecular data, including genotyping and haplotypic variability, on E. granulosus s.l. in dromedary camels from two regions in the extreme Sahara of Algeria. Future characterization of the G1, G3, and G6 samples based on sequencing of complete mitochondrial genomes would be of considerable significance for a more comprehensive understanding of molecular epidemiology of CE in Algeria.

[Cancer epidemiology in Algeria, 1996-2019]. / Épidémiologie des cancers en Algérie, 1996­2019.

INTRODUCTION: Cancer is a major public health problem in Algeria. The aim of this article was to estimate trends in cancer incidence in Oran, Algeria, over the period 1996-2019. PATIENTS AND METHODS: Nine cancer sites were analyzed. Incidence data over the 1996-2019 period were collected from the Oran cancer registry. The annual percent changes in incidence rates were evaluated with Joinpoint analysis. RESULTS: Between 1996 and 2019, unfavorable trends in incidence were observed for lung and colorectal cancers in both sexes, as well as female breast cancer. In contrast, a continuous decrease in incidence rates of cervical cancer were observed. As for the prostate, no corresponding trend was emerged for this cancer. CONCLUSION: The upward trend in incidence rates for some cancers underscores the need to strengthen prevention efforts.

Algerian Registry for Inborn Errors of Immunity in Children: Report of 887 Children (1985-2021).

INTRODUCTION: Inborn errors of immunity (IEI) represent a heterogeneous large group of genetic disorders characterized by susceptibility of affected individuals to recurrent infections, autoimmune/inflammatory diseases, allergy, and malignancy. We aimed to report for the first time the Algerian registry for IEI in children. METHODS: We described the characteristics of IEI in Algerian children from the data collected in the Algerian registry for IEI between 1985 and 2021. RESULTS: Over a period of 37 years, we included 887 children (530 male, 59.6%) with a mean age at diagnosis of 3.23 years and a mean diagnosis delay of 2 years. The prevalence rate was estimated at 1.97/100,000 inhabitants or 5.91/100,000 children. The parental consanguinity was found in 52.6%. The most prevalent category was combined immunodeficiencies (CID) (35.5%), followed by predominantly antibody deficiencies (24.5%) and CID with syndromic features (18.3%). The most predominant diseases were severe CID (134 cases), MHC II deficiency (99 cases), agammaglobulinemia (82 cases), common variable immunodeficiency (78 cases), hyper IgE syndromes (61 patients), ataxia-telangiectasia (46 patients), Wiskott-Aldrich syndrome (40 patients) and chronic granulomatous disease (39 cases). The clinical presentation was dominated by lower respiratory tract infections (69%), failure to thrive (38.3%), and chronic diarrhea (35.2%). Genetic analysis was performed in 156 patients (17.6%). The global mortality rate was 28.4% mainly caused by CID. CONCLUSION: This is the first report of the Algerian registry for IEI in children. Data is globally similar to that of the Middle East and North African (MENA) registries with high consanguinity, predominance of CID, and significant mortality. This registry highlights the weak points that should be improved in order to provide better patient care.

Genotype, Mortality, Morbidity, and Outcomes of 3ß-Hydroxysteroid Dehydrogenase Deficiency in Algeria.

Background: 3ß-hydroxysteroid dehydrogenase 2 (3ßHSD2) deficiency is a rare form of congenital adrenal hyperplasia (CAH), with fewer than 200 cases reported in the world literature and few data on outcomes. Patients and Methods: We report a mixed longitudinal and cross-sectional study from a single Algerian center between 2007 and 2021. Virilization and under-masculinization were assessed using Prader staging and the external masculinization score (EMS), pubertal development staged according to the system of Tanner. Adrenal steroids were measured using mass spectrophotometry (LC-MS/MS). A genetic analysis of HSD3B2 was performed using Sanger sequencing. Results: A 3ßHSD2 defect was confirmed in 6 males and 8 females from 10 families (8 consanguineous), with p.Pro222Gln mutation in all but two siblings with a novel deletion: c.453_464del or p.(Thr152_Pro155del). Probable 3ßHSD2 deficiency was diagnosed retrospectively in a further 6 siblings who died, and in two patients from two other centers. In the genetically confirmed patients, the median (range) age at presentation was 20 (0-390) days, with salt-wasting (n = 14) and genital anomaly (n = 10). The Prader stage for female patients was 2 (1-2) with no posterior fusion of the labia. The EMS for males was 6 (3-9). Median (range) values at diagnosis for 17-hydroxyprogesterone (17-OHP), dehydroepiandrosterone sulfate (DHEA-S), and 17-hydroxypregnenolone (17OHPreg) were elevated: 73.7 (0.37-164.3) nmol/L; 501.2(9.4-5441.3) nmol/L, and 139.7 (10.9-1500) nmol/l (NB >90 nmol/L diagnostic of 3ßHSD2 defect). Premature pubarche was observed in four patients (3F:1M). Six patients (5F:1M) entered puberty spontaneously, aged 11 (5-13) years in 5 girls and 11.5 years in one boy. Testicular adrenal rest tumors were found in three boys. Four girls reached menarche at 14.3 (11-14.5) years, with three developing adrenal masses (surgically excised in two) and polycystic ovary syndrome (PCOS), with radiological evidence of ovarian adrenal rest tumor in one. The median IQ was 90 (43-105), >100 in only two patients and <70 in three. Conclusions: The prevalence of 3ßHSD2 deficiency in Algeria appears high, with p.Pro222Gln being the most frequent mutation. Mortality is also high, with significant morbidity from adrenal tumors and PCOS in adolescence and an increased risk of learning disability. The finding of adrenal tumors in older patients with 3ßHSD2 indicates under-replacement, requiring effective hydrocortisone and fludrocortisone treatment rather than surgical removal.