Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana)

Abstract Guarana (Paullinia cupana) is a native plant from the Amazon whose seeds contain a high concentration of caffeine. Aqueous extract of guarana is widely used in the world. In this study, the objective was to develop and validate a High-Performance Liquid Chromatography method for the determination of caffeine in extracts and commercial beverages based on guarana. A sensitive, simple, and viable high performance liquid chromatographic method without the need of an analyte extraction procedure was developed and validated according to Brazilian and international requirements. The method presented high performance, fulfilling Brazilian and international requirements, in addition to allowing product compliance tests. Results confirmed high selectivity and linearity (>0.999) between 5 to 135 ug/mL, with no significant matrix effect. Detection and quantification limits were 0.02 µg/mL and 2 µg/mL, respectively. Precision was less than 4 %, and accuracy varied from 99.9-120 %. Applicability of the method was demonstrated by conducting a limited evaluation in products containing caffeine. Commercial extracts showed quite different caffeine levels, while carbonated drinks follow Brazilian and American recommendations. Our results indicate that the developed method can be used to evaluate the quality of the guarana extract and of products containing caffeine

Association between excess weight and beverage portion size consumed in Brazil / Associação entre excesso de peso e tamanho das porções de bebidas consumidas no Brasil

ABSTRACT OBJECTIVE To describe the beverage portion size consumed and to evaluate their association with excess weight in Brazil. METHODS We used data from the National Dietary Survey, which included individuals with two days of food record aged over 20 years (n = 24,527 individuals). The beverages were categorized into six groups: soft drink, 100% fruit juice, fruit drink, alcoholic beverage, milk, and coffee or tea. We estimated the average portion consumed for each group and we evaluated, using linear regression, the association between portion size per group and the variables of age, sex, income, and nutritional status. We tested the association between portion size and excess weight using Poisson regression, adjusted for age, sex, income, and total energy intake. RESULTS The most frequently consumed beverages in Brazil were coffee and tea, followed by 100% fruit juices, soft drinks, and milk. Alcoholic beverages presented the highest average in the portion size consumed, followed by soft drinks, 100% fruit juice, fruit drink, and milk. Portion size showed positive association with excess weight only in the soft drink (PR = 1.19, 95%CI 1.10-1.27) and alcoholic beverage groups (PR = 1.20, 95%CI, 1.11-1.29), regardless of age, sex, income, and total energy intake. CONCLUSIONS Alcoholic beverages and soft drinks presented the highest averages in portion size and positive association with excess weight. Public health interventions should address the issue of portion sizes offered to consumers by discouraging the consumption of large portions, especially sweetened and low nutritional beverages.

RESUMO OBJETIVO Descrever o tamanho das porções de bebidas consumidas e avaliar sua associação com excesso de peso no Brasil. MÉTODOS Utilizou-se dados do Inquérito Nacional de Alimentação, incluindo indivíduos com dois dias de registro alimentar, acima de 20 anos de idade (n = 24.527 indivíduos). As bebidas foram categorizadas em seis grupos: refrigerante; suco; refresco; bebida alcóolica; leite; e café ou chá. Estimou-se a porção média por ocasião de consumo para cada grupo e avaliou-se, por meio de regressão linear, a associação entre tamanho da porção por grupo e as variáveis idade, sexo, renda e estado nutricional. A associação entre tamanho da porção e excesso de peso foi testada por meio de regressão de Poisson, ajustada por idade, sexo, renda e ingestão total de energia. RESULTADOS As bebidas com maior frequência de consumo no Brasil foram café e chá, seguidas dos sucos, refrigerantes e leite. As bebidas alcóolicas apresentaram maior média no tamanho da porção consumida, seguidas dos refrigerantes, sucos, refrescos e leite. O tamanho da porção mostrou associação positiva com excesso de peso somente no grupo dos refrigerantes (RP = 1,19; IC95% 1,10-1,27), e bebidas alcoólicas (RP = 1,20; IC95% 1,11-1,29), independentemente da idade, sexo, renda e ingestão total de energia. CONCLUSÕES Bebidas alcoólicas e refrigerantes apresentaram as maiores médias no tamanho da porção e associação positiva com excesso de peso. Intervenções de saúde pública devem abranger a questão do tamanho das porções oferecidas aos consumidores, desencorajando o consumo de grandes porções, principalmente das bebidas adicionadas de açúcar e de baixo teor nutricional.

Efecto de diferentes bebidas en la estabilidad de color de las resinas compuestas para restauraciones directas / Th effect of different beverages on color stability of composite resins for direct restorations

Las resinas compuestas constituyen un grupo de materiales restauradores utilizados debido a sus propiedades estéticas, físicas y mecánicas. Sin embargo, un gran número de factores, como la dieta, oclusión, fallas en la técnica de obturación y caries, pueden llevar al fracaso. Este trabajo tiene el propósito de determinar la existencia de cambios de color de distintos tipos de resinas compuestas para restauraciones directas, luego de su exposición a diferentes bebidas de consumo habitual. Se realizó un diseño experimental “in vitro”, transfiriéndolo a un “diseño clínico”. Para la interpretación de los resultados clínicos se seleccionó un análisis estadístico que tenga en cuenta los grupos de resinas utilizadas. De ahí se obtuvieron valores en cuanto a cambios de color en los distintos grupos sumergidos en diferentes sustancias. En los grupos I (Z 350 3M), II (Brillant ) y III (Amaris) puede afirmarse que, al sumergir las piezas dentarias (in vitro) en vino tinto, infusión de mate y bebidas colas, fueron significativamente más pigmentadas que las sumergidas en una solución de cloramina T como grupo control. Sin embargo, en los tres casos clínicos hubo cambios de color, pero se mantuvo dentro de las tonalidades rojizas amarillentas y parduscas.

The composite based resins ave a group of restorative materials used due to their aesthetic, physical and mechanical properties. However, a great number of factors, such as diet, occlusion, restorative technique faults, and cavities, may lead to failure. The main of this study, is to determinate the different composite resins changing color for direct restorations, after his exposure to current drinks. It has been made an experimental “in vitro” design, transferring it to a “Clinical design”. For the interpretation of clinical results, a statistical analysis was selected, which considers the groups of used resins, getting values in terms of changes colors in the diverse groups submersed in different substances. In the groups I (Z 350 3M), II (Brillant) and III (Amaris), con be affirmed that when the teeth were submerged into red wine, mate infusion, and coke drinks, were significantly more pigmented those which were submerged in chloramine T as a control group. Nevertheless, in the three clinical cases, there was change of color, but staying within the reddish, yellowish and Brown shades.

Chemometric classification of apple juices according to variety and geographical origin based on polyphenolic profiles.

To characterize and classify apple juices according to apple variety and geographical origin on the basis of their polyphenol composition, the polyphenolic profiles of 58 apple juice samples belonging to 5 apple varieties and from 6 regions in Shaanxi province of China were assessed. Fifty-one of the samples were from protected designation of origin (PDO) districts. Polyphenols were determined by high-performance liquid chromatography coupled to photodiode array detection (HPLC-PDA) and to a Q Exactive quadrupole-Orbitrap mass spectrometer. Chemometric techniques including principal component analysis (PCA) and stepwise linear discriminant analysis (SLDA) were carried out on polyphenolic profiles of the samples to develop discrimination models. SLDA achieved satisfactory discriminations of apple juices according to variety and geographical origin, providing respectively 98.3 and 91.2% success rate in terms of prediction ability. This result demonstrated that polyphenols could served as characteristic indices to verify the variety and geographical origin of apple juices.

Factors associated with reduced compliance of children to dental preventive measures.

The aim of this study was to record self-reported factors for non-compliance to 'dental home' preventive measures among pediatric patients, and to clarify whether number or frequency of recall appointments increases their long-term compliance to dental home. Children attending recall appointments in a pediatric dental clinic were interviewed regarding their compliance as well as factors for non-compliance with the previously recommended preventive measures. Files of 651 children were analyzed. A significant positive correlation was found between compliance with regular teeth brushing twice a day (p < 0.0001), using correct fluoride concentration toothpaste (p < 0.0001), drinking water between meals (p < 0.0001), using Elmex gel (p < 0.0001), flossing (p < 0.002) and number of recall appointments. The most common factor for non-compliance to 'dental home' was 'I forgot'. Not flossing was commonly justified by performance difficulty while non-compliance to Elmex gel or to mouth rinses was attributed mainly to 'bad taste'. Additional common reasons were 'it was finished', 'my parents did not purchase', 'I did not find it at home', 'I did not know I need to'. Frequency and number of recall appointments might improve compliance with dental preventive measures. Referring to the specific factors of personal non-compliance may increase compliance.

Effect of beverages on bovine dental enamel subjected to erosive challenge with hydrochloric acid

This study evaluated by an in vitro model the effect of beverages on dental enamel previously subjected to erosive challenge with hydrochloric acid. The factor under study was the type of beverage, in five levels: Sprite® Zero Low-calorie Soda Lime (positive control), Parmalat® ultra high temperature (UHT) milk, Ades® Original soymilk, Leão® Ice Tea Zero ready-to-drink low-calorie peach-flavored black teaand Prata® natural mineral water (negative control). Seventy-five bovine enamel specimens were distributed among the five types of beverages (n=15), according to a randomized complete block design. For the formation of erosive wear lesions, the specimens were immersed in 10 mL aqueous solution of hydrochloric acid 0.01 M for 2 min. Subsequently, the specimens were immersed in 20 mL of the beverages for 1 min, twice daily for 2 days at room temperature. In between, the specimens were kept in 20 mL of artificial saliva at 37ºC. The response variable was the quantitative enamel microhardness. ANOVA and Tukey's test showed highly significant differences (p<0.00001) in the enamel exposed to hydrochloric acid and beverages. The soft drink caused a significantly higher decrease in microhardness compared with the other beverages. The black tea caused a significantly higher reduction in microhardness than the mineral water, UHT milk and soymilk, but lower than the soft drink. Among the analyzed beverages, the soft drink and the black tea caused the most deleterious effects on dental enamel microhardness.

Este estudo avaliou através de um modelo in vitro o efeito de bebidas no esmalte dental previamente submetido a desafio erosivo com ácido clorídrico. O fator em estudo foi o tipo de bebida, em cinco níveis: Sprite Zero (Coca-Cola® Brasil) controle positivo, leite integral Ultra High Temperature (Parmalat®), extrato de soja integral (Ades® Original), chá preto com baixo teor calórico sabor pêssego (Leão Ice Tea Zero Coca-Cola® Brasil) e água mineral Prata® (controle negativo). Setenta e cinco espécimes de esmalte bovino foram distribuídos entre os cinco tipos de bebidas (n = 15), de acordo com delineamento em blocos completos e casualizados. Para a formação das lesões de desgaste erosivo, os espécimes foram imersos durante 2 min em 10 mL de solução aquosa de ácido clorídrico 0,01 M. Subsequentemente foram imersos em 20 mL das bebidas por 1 min, duas vezes ao dia durante 2 dias, em temperatura ambiente. Nos intervalos entre as imersões os espécimes permaneceram em saliva artificial a 37ºC. A variável de resposta quantitativa foi microdureza superficial do esmalte. ANOVA e teste de Tukey demonstraram diferenças altamente significativas (p <0,00001) no esmalte exposto ao ácido clorídrico e às bebidas. O refrigerante provocou diminuição da microdureza superficial significativamente maior do que as demais bebidas. O chá preto provocou diminuição da microdureza significativamente maior que a água mineral, o leite UHT e o extrato de soja, porém menor que o refrigerante.Entre as bebidas analisadas, refrigerante e chá preto demonstraram efeitos mais deletérios sobre a microdureza do esmalte dental.

Dietary cadmium intake by the Belgian adult population.

The aim of this study was to estimate the dietary cadmium (Cd) intake of the Belgian adult population, to compare this dietary Cd exposure to the tolerable weekly intake (TWI) recently established by the European Food Safety Authority (EFSA) and to determine the major food groups that contribute to dietary Cd exposure in Belgium. Food consumption data were derived from the 2004 Belgian food consumption survey (two 24 h recalls, 3083 participants). Cadmium concentrations in food items (n = 4000) were gathered from the control program of the Belgian Federal Agency for the Safety of the Food Chain for the period 2006-2008. Dietary intake per individual was calculated from consumption data and median Cd concentrations. The population mean, median and 95th percentile of the dietary intake values were 0.98, 0.85 and 2.02 µg kg⁻¹ body weight per week respectively. Two percent of the Belgian adult population has a dietary Cd intake above the recent TWI of 2.5 µg kg⁻¹ body weight established by EFSA in 2009. Cereal products and potatoes contribute for more than 60% to Cd intake.

The shifting beverage landscape.

STOREY, M.L. The shifting beverage landscape. PHYSIOL BEHAV, 2010. - Simultaneous lifestyle changes have occurred in the last few decades, creating an imbalance in energy intake and energy expenditure that has led to overweight and obesity. Trends in the food supply show that total daily calories available per capita increased 28% since 1970. Total energy intake among men and women has also increased dramatically since that time. Some have suggested that intake of beverages has had a disproportional impact on obesity. Data collected by the Beverage Marketing Corporation between 1988-2008 demonstrate that, in reality, fewer calories per ounce are being produced by the beverage industry. Moreover, data from the National Cancer Institute show that soft drink intake represents 5.5% of daily calories. Data from NHANES 1999-2003 vs. 2003-06 may demonstrate a shift in beverage consumption for age/gender groups, ages 6 to>60years. The beverages provided in schools have significantly changed since 2006 when the beverage industry implemented School Beverage Guidelines. This voluntary action has removed full-calorie soft drinks from participating schools across the country. This shift to lower-calorie and smaller-portion beverages in school has led to a significant decrease in total beverage calories in schools. These data support the concept that to prevent and treat obesity, public health efforts should focus on energy balance and that a narrow focus on sweetened beverages is unlikely to have any meaningful impact on this complex problem.

International multidimensional authenticity specification (IMAS) algorithm for detection of commercial pomegranate juice adulteration.

The pomegranate fruit ( Punica granatum ) has become an international high-value crop for the production of commercial pomegranate juice (PJ). The perceived consumer value of PJ is due in large part to its potential health benefits based on a significant body of medical research conducted with authentic PJ. To establish criteria for authenticating PJ, a new International Multidimensional Authenticity Specifications (IMAS) algorithm was developed through consideration of existing databases and comprehensive chemical characterization of 45 commercial juice samples from 23 different manufacturers in the United States. In addition to analysis of commercial juice samples obtained in the United States, data from other analyses of pomegranate juice and fruits including samples from Iran, Turkey, Azerbaijan, Syria, India, and China were considered in developing this protocol. There is universal agreement that the presence of a highly constant group of six anthocyanins together with punicalagins characterizes polyphenols in PJ. At a total sugar concentration of 16 degrees Brix, PJ contains characteristic sugars including mannitol at >0.3 g/100 mL. Ratios of glucose to mannitol of 4-15 and of glucose to fructose of 0.8-1.0 are also characteristic of PJ. In addition, no sucrose should be present because of isomerase activity during commercial processing. Stable isotope ratio mass spectrometry as > -25 per thousand assures that there is no added corn or cane sugar added to PJ. Sorbitol was present at <0.025 g/100 mL; maltose and tartaric acid were not detected. The presence of the amino acid proline at >25 mg/L is indicative of added grape products. Malic acid at >0.1 g/100 mL indicates adulteration with apple, pear, grape, cherry, plum, or aronia juice. Other adulteration methods include the addition of highly concentrated aronia, blueberry, or blackberry juices or natural grape pigments to poor-quality juices to imitate the color of pomegranate juice, which results in abnormal anthocyanin profiles. To adjust the astringent taste of poor-quality juice or peel extract, addition of nonpomegranate sugars is a commonly detected adulteration method. The profile generated from these analyses combined with information from existing databases and published literature has been integrated into a validated IMAS for PJ, which can be utilized to detect PJ adulteration. In this survey of commercial pomegranate juices, only 6 of 23 strictly met all of the IMAS criteria.

Consumo de bebidas para una vida saludable: recomendaciones para la población mexicana / Drink consumption for a healthy life: recommendations for the general population in Mexico

El Secretario de Salud convocó al Comité de Expertos para la elaboración de las “Recomendaciones sobre el consumo de bebidas para la población mexicana”; la finalidad fue desarrollar lineamientos basados en evidencia científica para los consumidores, los profesionales de la salud y el sector gubernamental. Las prevalencias de sobrepeso, obesidad y diabetes han aumentado con rapidez en México y las bebidas representan la quinta parte de la energía que consumen los mexicanos. La evidencia señala que las bebidas con aporte energético incrementan el riesgo de obesidad. Considerando los beneficios y riesgos para la salud y nutrición, así como el patrón de consumo de las bebidas en México, el Comité clasificó las bebidas en seis categorías de acuerdo con su contenido energético, valor nutricio y riesgos a la salud, en una escala que clasifica las bebidas de la más (nivel 1) a la menos (nivel 6) saludable. Nivel 1: agua potable; nivel 2: leche baja en grasa (1%) y sin grasa y bebidas de soya sin azúcar; nivel 3: café y té sin azúcar; nivel 4: bebidas no calóricas con edulcorantes artificiales; nivel 5: bebidas con alto valor calórico y beneficios a la salud limitados (jugos de fruta, leche entera, licuados de fruta con azúcar o miel, bebidas alcohólicas y bebidas deportivas); y nivel 6: bebidas con azúcar y bajo contenido de nutrimentos (refrescos y otras bebidas con altas cantidades de azúcares agregadas como jugos, aguas frescas, café y té). El Comité recomienda el consumo de agua en primer lugar, seguido de bebidas sin o con bajo aporte energético y leche descremada. Éstas deben tener prioridad sobre las de mayor aporte energético o endulzadas, incluso con edulcorantes artificiales. Se presentan cantidades recomendadas para cada categoría de bebidas y se ilustran patrones de consumo saludable para adultos de ambos sexos.

The Expert Committee in charge of developing the Beverage Consumption Recommendations for the Mexican Population was convened by the Ministry of Health with the aim of drafting evidence-based guidelines for consumers, health professionals, and government officials. The prevalence of overweight, obesity and diabetes have dramatically increased in Mexico; beverages contribute a fifth of all calories consumed by Mexicans. Extensive research has documented that caloric beverages increase the risk of obesity. Taking into consideration multiple factors, including health benefits, risks, and nutritional implications associated with beverage consumption, as well as consumption patterns in Mexico, the committee classified beverages in six categories. Classifications were made based on caloric content, nutritional value, and health risks associated with the consumption of each type of beverage. Ranges included healthier (level 1) to least healthy (level 6) options as follows: Level 1: water; Level 2: skim or low fat (1%) milk and sugar free soy beverages; Level 3: coffee and tea without sugar; Level 4: non-caloric beverages with artificial sweeteners; Level 5: beverages with high caloric content and limited health benefits (fruit juices, whole milk, and fruit smoothies with sugar or honey; alcoholic and sports drinks), and Level 6: beverages high in sugar and with low nutritional value (soft drinks and other beverages with significant amounts of added sugar like juices, flavored waters, coffee and tea). The committee recommends the consumption of water as a first choice, followed by no or low-calorie drinks, and skim milk. These beverages should be favored over beverages with high caloric value or sweetened beverages, including those containing artificial sweeteners. Portion size recommendations are included for each beverage category together with healthy consumption patterns for men and women.

Consumo de bebidas para una vida saludable: recomendaciones para la población mexicana / Beverage consumption for a healthy life: recommendations for the Mexican population

El secretario de Salud convocó al Comité de Expertos para la elaboración de las "Recomendaciones sobre el consumo de bebidas para la población mexicana"; la finalidad fue desarrollar lineamientos basados en evidencia científica para los consumidores, los profesionales de la salud y el sector gubernamental. Las prevalencias de sobrepeso, obesidad y diabetes han aumentado con rapidez en México y las bebidas representan la quinta parte de la energía que consumen los mexicanos. La evidencia señala que las bebidas con aporte energético incrementan el riesgo de obesidad. Considerando los beneficios y riesgos para la salud y nutrición, así como el patrón de consumo de las bebidas en México, el Comité clasificó las bebidas en seis categorías de acuerdo con su contenido energético, valor nutricio y riesgos a la salud en una escala que clasifica las bebidas de la más (nivel 1) a la menos (nivel 6) saludable. Nivel 1: agua potable; nivel 2: leche baja en grasa (1 por ciento) y sin grasa y bebidas de soya sin azúcar; nivel 3: café y té sin azúcar; nivel 4: bebidas no calóricas con edulcorantes artificiales; nivel 5: bebidas con alto valor calórico y beneficios a la salud limitados (jugos de fruta, leche entera, licuados de fruta con azúcar o miel, bebidas alcohólicas y bebidas deportivas); y nivel 6: bebidas con azúcar y bajo contenido de nutrimentos (refrescos y otras bebidas con altas cantidades de azúcares agregadas como jugos, aguas frescas, café y té). El Comité recomienda el consumo de agua en primer lugar, seguido de bebidas sin o con bajo aporte energético y leche descremada. Éstas deben tener prioridad sobre las de mayor aporte energético o endulzadas, incluso con edulcorantes artificiales. Se presentan cantidades recomendadas para cada categoría de bebidas y se ilustran patrones de consumo saludable para adultos de ambos sexos.

The Expert Committee in charge of developing the Beverage Consumption Recommendations for the Mexican Population was convened by the Secretary of Health for the purpose of developing evidence-based guidelines for consumers, health professionals, and government officials. The prevalence of overweight, obesity and diabetes have dramatically increased in Mexico; beverages contribute a fifth of all calories consumed by Mexicans. Extensive research has found that caloric beverages increase the risk of obesity. Taking into consideration multiple factors, including the health benefits, risks, and nutritional implications associated with beverage consumption, as well as consumption patterns in Mexico, the committee classified beverages into six levels. Classifications were made based on caloric content, nutritional value, and health risks associated with the consumption of each type of beverage and range from the healthier (level 1) to least healthy (level 6) options, as follows: Level 1: water; Level 2: skim or low fat (1 percent) milk and sugar free soy beverages; Level 3: coffee and tea without sugar; Level 4: non-caloric beverages with artificial sweeteners; Level 5: beverages with high caloric content and limited health benefits (fruit juices, whole milk, and fruit smoothies with sugar or honey; alcoholic and sports drinks), and Level 6: beverages high in sugar and with low nutritional value (soft drinks and other beverages with significant amounts of added sugar like juices, flavored waters, coffee and tea). The committee recommends the consumption of water as a first choice, followed by no or low-calorie drinks, and skim milk. These beverages should be favored over beverages with high caloric value or sweetened beverages, including those containing artificial sweeteners. Portion size recommendations are included for each beverage category and healthy consumption patterns for men and women are illustrated.

Food fight: the battle over redefining competitive foods.

BACKGROUND: Environmental and policy influences are potentially the most powerful-and yet the least understood-strategies for reversing the current childhood obesity epidemic. METHODS: This essay focuses on the school lunch environment and examines the key legal and policy factors that affect competitive foods or foods of minimal nutritional value (FMNV) in schools. The essay also analyzes the latest, proposed federal legislation on redefining competitive foods. RESULTS: FMNV compete for children's coins and calories in the school food environment. The emerging scientific record on the negative impact competitive foods have on children's diet and health is significantly stronger than when Congress and the United States Department of Agriculture (USDA) secretary first deemed the connection sufficient to take action. The Child Nutrition Promotion and School Lunch Protection Act of 2006, if enacted, will provide additional authority to the USDA and supply science-based federal guidance to local schools. Opposition to this legislation may come from the local school districts, the food industry, parents, and children. CONCLUSIONS: Defining competitive foods in a federally consistent manner and eliminating competitive foods from our nation's schools are food fight that will evidently be fought by legislative efforts at the local, state, and federal level. The food industry, as history illustrates, will likely work hard to weaken any regulatory efforts and could potentially demand that this fight end up requiring judicial review.

A content analysis of health and physical activity messages marketed to African American children during after-school television programming.

OBJECTIVE: To examine the number of food advertisements African American children are exposed to during children's television programming aired on predominantly African American and general television stations. DESIGN: A content analysis was conducted to identify and analyze the health-related content (HRC) and physical activity-related content (PARC) of food advertisements shown during children's television programming. SETTING: Three sets of television advertisements from 3 stations (Black Entertainment Television, The WB [Warner Bros], and Disney Channel) served as the sample during a 1-week period in July 2005 (July 11-15), from 3 pm to 9 pm. RESULTS: In total, 1098 advertisements were recorded, with 256 food and beverage commercials used for this study. Results indicate that 36.3% of all commercials were based on fast food restaurants, 31.3% were for drinks, 16.8% were for candy, 13.7% were for cereals, and 2.0% were for snacks (percentages do not total 100 because of rounding). Compared with The WB and Disney Channel, Black Entertainment Television had significantly (P=.001) more food and beverage advertisements. Few HRC or PARC advertisements were shown. Of 256 food and beverage commercials, only 8.2% contained HRC and 9.4% had PARC. Also, the HRC and PARC scenes contained messages that were implied vs explicitly talking about the health or physical benefits of the product. CONCLUSIONS: African American children are overexposed to numerous types of food and beverage advertisements. These advertisements do not provide an adequate level of positive HRC and PARC messages. Consequently, the messages that are portrayed may undermine efforts to teach African American children about the importance of healthy living and physical activity.

Preliminary studies for the differentiation of apple juice samples by chemometric analysis of solid-phase microextraction-gas chromatographic data.

A combination of gas chromatography (GC) and chemometrics was evaluated for its ability to differentiate between apple juice samples on the basis of apple variety and applied heat treatment. The heat treatment involved exposure of 15 mL juice samples for 30 s in a 900 W domestic microwave oven. The chromatographic results were subjected to two chemometric procedures: (1) partial least squares (PLS) regression and (2) linear discriminant analysis (LDA) applied to principal component (PC) scores. The percent correct classification of samples were obtained from PLS and LDA in terms of separation on the basis of apple variety and applied heat treatment. PLS gave the highest level of correct classification of the apple juice samples according to both variety and heat treatment, 92.5% correct classification in each case. When LDA was performed on the PC scores obtained from GC analysis, 87.5% and 80% of samples were correctly classified according to apple variety used and applied heat treatment, respectively.

Breast-feeding at 12 months of age and dietary habits among breast-fed and non-breast-fed infants.

OBJECTIVE: To analyse factors associated with breast-feeding and use of sweetened drinks at 12 months, and to compare dietary habits among breast-fed and non-breast-fed infants. DESIGN: Data were collected by a semi-quantitative food-frequency questionnaire filled in by the parents. SETTING: National dietary survey in Norway. SUBJECTS: In total, 1932 12-month-old infants were included. RESULTS: At 12 months, 36% of the infants were breast-fed. The odds of breast-feeding at this age were more than doubled both for mothers > or =35 years compared with mothers <25 years and for mothers in the highest educational group compared with mothers in the lowest. A negative association was found for maternal smoking, and the odds of breast-feeding were 40% lower for mothers who smoked than for non-smokers. Some dietary differences were observed between breast-fed and non-breast-fed infants apart from intake of milk. In particular, breast-fed infants had a significantly lower daily intake of sweetened drinks than non-breast-fed infants and a 16% lower mean daily intake of added sugars (P<0.001). Furthermore, breast-fed infants had 30% higher odds of not receiving sweetened drinks daily, compared with non-breast-fed infants. CONCLUSIONS: Maternal age, education and smoking status were important factors for breast-feeding at 12 months. Breast-fed infants had lower intakes of sweetened drinks and added sugars than non-breast-fed infants. From a public health perspective, continued promotion of breast-feeding is needed to reduce inequalities in breast-feeding. Moreover, prevention of high intakes of sweetened drinks and added sugars should start in infancy.

Fluid intake and the incidence of bladder cancer among middle-aged men and women in a three-county area of western Washington.

This population-based case-control study reports on the relationship between fluid intake and the incidence of bladder cancer among 262 bladder cancer cases from Western Washington and 405 controls identified through random-digit dialing Cases were identified from the Surveillance. Epidemiology, and End Results (SEER) registry and were diagnosed between January 1987 and June 1990. All eligible subjects were Caucasian 45- to 65-year-old residents of King, Pierce, or Snohomish counties and completed a structured telephone interview. Analyses were conducted by logistic regression with adjustment for age, county, and smoking (current, former, never). Among women there was a positive association between total fluid intake and the incidence of bladder cancer [p (trend = 0.02] and a moderate positive association between the use of decaffeinated coffee and the incidence of bladder cancer [p (trend) = 0.08]. Among men there was an inverse association between the consumption of regular soft drinks and the incidence of bladder cancer [p (trend) = 0.03]. No association was found between the incidence of bladder cancer and the intake of water, coffee, tea, diet soft drinks, alcohol, or liquids from tap for men or women. This study suggests that the intake of water and specific beverages is overall not associated with risks of bladder cancer. This study provides limited evidence of a positive association between total fluid intake and bladder cancer among women.

A choice-based method to compare alternative alcohol warning labels.

Alternative alcohol warning labels were studied with a method used in magical-thinking research. In two studies (N = 111, N = 75), subjects completed a questionnaire. A "poison," "toxic" or "causes cancer" label on a beer can was shown to have substantial effects on self-reported choice behavior. In contrast, the alcohol warning label now mandated to appear on alcohol containers did not have nearly as powerful an effect. Explanations for the lack of strong effects of the mandated warning label center around label length, type of warning and salience of the warning. The methodology described in this article appears to test gut-level or conditioned responses to labels, which may be a valuable technique for evaluating warning labels.