Assuntos
Antineoplásicos Alquilantes/efeitos adversos , Medula Óssea/patologia , Leucemia Mieloide Aguda/induzido quimicamente , Leucemia Mieloide Aguda/genética , Melfalan/efeitos adversos , Mieloma Múltiplo/tratamento farmacológico , Cariótipo Anormal/induzido quimicamente , Idoso , Antineoplásicos Alquilantes/uso terapêutico , Medula Óssea/efeitos dos fármacos , Medula Óssea/metabolismo , Humanos , Leucemia Mieloide Aguda/patologia , Masculino , Melfalan/uso terapêuticoRESUMO
The effect of root extract of Baikal skullcap (Scutellaria baicalensis) cultivated in vitro, on the gene structure of CBA/CaLac mice bone marrow cells damaged by anticancer drugs paclitaxel and cisplatin has been studied. It is established that the root extract exhibits gene protective property upon both single and chronic administration.
Assuntos
Cariótipo Anormal , Antineoplásicos Fitogênicos/efeitos adversos , Células da Medula Óssea/metabolismo , Cisplatino/efeitos adversos , Paclitaxel/efeitos adversos , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Cariótipo Anormal/induzido quimicamente , Cariótipo Anormal/efeitos dos fármacos , Animais , Antineoplásicos Fitogênicos/farmacologia , Células da Medula Óssea/patologia , Cisplatino/farmacologia , Feminino , Masculino , Camundongos , Paclitaxel/farmacologia , Extratos Vegetais/química , Scutellaria baicalensis/químicaRESUMO
BACKGROUND: The identification and characterization of cancer stem cells (CSCs) is imperative to understanding the mechanism of cancer pathogenesis. Growing evidence suggests that CSCs play critical roles in the development and progression of cancer. However, controversy exists as to whether CSCs arise from bone marrow-derived cells (BMDCs). METHODOLOGY AND PRINCIPAL FINDINGS: In the present study, n-nitrosodiethylamine (DEN) was used to induce tumor formation in female mice that received bone marrow from male mice. Tumor formation was induced in 20/26 mice, including 12 liver tumors, 6 lung tumors, 1 bladder tumor and 1 nasopharyngeal tumor. Through comparison of fluorescence in situ hybridization (FISH) results in corresponding areas from serial tumor sections stained with H&E, we determined that BMDCs were recruited to both tumor tissue and normal surrounding tissue at a very low frequency (0.2-1% in tumors and 0-0.3% in normal tissues). However, approximately 3-70% of cells in the tissues surrounding the tumor were BMDCs, and the percentage of BMDCs was highly associated with the inflammatory status of the tissue. In the present study, no evidence was found to support the existence of fusion cells formed form BMDCs and tissue-specific stem cells. CONCLUSIONS: In summary, our data suggest that although BMDCs may contribute to tumor progression, they are unlike to contribute to tumor initiation.