RESUMO
Clostridium tyrobutyricum shows probiotic properties and can affect the composition of gut microbiota and regulate the intestinal immune system. Compared with other probiotics, this spore-producing bacterium shows unparalleled advantages in commercial production. In addition to being resistant to extreme living environments for extended periods, its endophytic spores are implicated in inhibiting cancer cell growth. We speculated that C. tyrobutyricum spores can also promote gut health, which mean it can maintain intestinal homeostasis. To date, the beneficial effects of C. tyrobutyricum spores on gut health have not been reported. In this study, a Spo0A-overexpressing C. tyrobutyricum strain was developed to increase spore production, and its probiotic effects on the gut were assessed. Compared with the wild-type, the engineered strain showed significantly increased sporulation rates. Mice administered with the engineered strain exhibited enhanced intestinal villi and the villus height/crypt depth ratio, weight gain and improved Firmicutes/Bacteroidetes ratio to facilitate intestinal homeostasis. This study demonstrated for the first time that enhanced spore production in C. tyrobutyricum can improve intestinal homeostasis, which is advantageous for its commercial application in food and pharmaceutical industry.
Assuntos
Bacteroidetes/crescimento & desenvolvimento , Clostridium tyrobutyricum/fisiologia , Firmicutes/crescimento & desenvolvimento , Microbioma Gastrointestinal , Intestino Delgado/microbiologia , Esporos Bacterianos/crescimento & desenvolvimento , Fatores de Transcrição/genética , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Bacteroidetes/classificação , Peso Corporal , Clostridium tyrobutyricum/genética , Clostridium tyrobutyricum/crescimento & desenvolvimento , Firmicutes/classificação , Expressão Gênica , Homeostase , Mucosa Intestinal/anatomia & histologia , Mucosa Intestinal/microbiologia , Masculino , Camundongos , Viabilidade Microbiana , Plasmídeos , Probióticos , Fatores de Transcrição/metabolismo , Transformação BacterianaAssuntos
Ácido Butírico/metabolismo , Clostridium tyrobutyricum/genética , Clostridium tyrobutyricum/metabolismo , Fosfofrutoquinase-1/genética , Piruvato Quinase/genética , Trifosfato de Adenosina/metabolismo , Clostridium tyrobutyricum/crescimento & desenvolvimento , Fermentação , Regulação Enzimológica da Expressão Gênica , Glucose/metabolismo , Glicólise , NAD/metabolismoRESUMO
The carbon metabolism of newly isolated Clostridium tyrobutyricum JM1 was investigated at varying initial glucose concentrations (27.8-333.6mM). Because an understanding of metabolic regulations was required to provide guidance for further effective metabolic design or optimization, in this case, maximizing hydrogen production, carbon material, and energy balances by C. tyrobutyricum JM1 were determined and applied in anaerobic glucose metabolism. The overall carbon distribution suggested that initial glucose concentrations had strong influence on the stoichiometric coefficients of products and the molar production of ATP on the formation of biomass. C. tyrobutyricum JM1 had a high capacity for hydrogen production at the initial glucose concentration of 222.4 mM with high concentrations of acetate and butyrate.