Health education can save the environment from medicine residues

Abstract The incorrect disposal of medicines and their environmental impact has been related to the health medicalization and the improper use of medication by society. In this sense, it is very important to know the profile of drug disposal for foster health policies. The aim was to identify the profile of disposal of medicines by the population, including the cost perspective. This is an inquiry descriptive study that began in September 2019. Medicine disposal health education program was carried out over six months in two University pharmacies. A questionnaire for sociodemographic and discarded medicines data collection was applied. Logistic regression analysis for variables association of correct disposal and the chi-square and t-student analysis for comparison between disposal programs were performed for a level of 5% and test power of 80%. Medicines weighed 23.3 kg and 28.5 kg, with the cost variation from US$ 13.5 to US$ 16.1 until the final treatment. The correct disposal was strongly associated with the disposal reason (p=0.013), source of information (p=0.006), prescription (p=0.03), form of use (p=0.01), acquisition source (p=0.001), cost with medication (p=0.0001), education (p=0.028) and age (p=0.05). The correct medicine disposal was associated with important features of the community related to education health.

Potential interactions between psychotropic drugs and alcohol and tobacco dependence

Abstract The aim of this study was to identify and analyze the potential interactions between psychotropic drugs and alcohol and tobacco addiction. A cross-sectional study was carried out on secondary data collection in a Center for Psychosocial Care in Alcohol and Other Drugs. Subjects aged 18 years old and over, with alcohol and tobacco dependence, who were taking psychotherapies were included. Medical records with the most recent prescriptions were reviewed. Potential interactions between psychotropic drugs and alcohol and tobacco were analyzed using the Micromedex database and stratified according to clinical risks and mechanisms of action. The Pearson's Chi-square test was used to find significant associations between the variables of interest. The significance level was set at 5%. Between the 2010-2018 period, 2,322 subjects were treated at the care center. Of these, 1,020 fulfilled the inclusion criteria, out of whom 515 (50.5%) were dependent on alcohol and 310 (30.4%) were dependent on tobacco. We found 1,099 potential interactions between psychotropic drugs and alcohol and 160 potential interactions between psychotropic drugs and tobacco. In relation to alcohol dependence, psychotropic drugs interacted largely with moderate clinical risk, and pharmacokinetic mechanisms of action. In relation to tobacco dependence, high clinical risk interactions and pharmacodynamic mechanisms of action predominated.

Sodium alendronate: proposal and reliability of indicators

Abstract The present study proposes and evaluates the test-retest reliability of indicators of the correct use of sodium alendronate in elderly patients. This is a test-retest reliability study for use of sodium alendronate. Six questions to evaluate the correct use of this medicine were elaborated after analysis of information in the literature. Data collection was performed through questionnaires in face-to-face in-home interviews by previously trained interviewers. The participants were initially interviewed (test) when they agreed to participate in the study, and secondly (retest), after a period of 7 to 14 days from the first interview. The reliability of the questions was evaluated by means of the agreement percentage and the Kappa coefficient. Fifty-seven pairs (test-retest) were obtained. The mean age was 69.3 (SD = 6.9) years, the majority (92.5%) completed elementary education, and declared themselves white (50.9%). All the questions presented high concordance ranging from 79.0% to 98.3%. The Kappa values ranged from 0.1 (low) to 0.83 (very good). The agreement percentage and the Kappa values suggest adequate reliability of the proposed questions. We suggest that they can be used as a simple and quick way to evaluate the quality of sodium alendronate use among the elderly.

Surgical data recording in the operating room: a systematic review of modalities and metrics.

INTRODUCTION: Operating room recording, via video, audio and sensor-based recordings, is increasingly common. Yet, surgical data science is a new field without clear guidelines. The purpose of this study is to examine existing published studies of surgical recording modalities to determine which are available for use in the operating room, as a first step towards developing unified standards for this field. METHODS: Medline, EMBASE, CENTRAL and PubMed databases were systematically searched for articles describing modalities of data collection in the operating room. Search terms included 'video-audio media', 'bio-sensing techniques', 'sound', 'movement', 'operating rooms' and others. Title, abstract and full-text screening were completed to identify relevant articles. Descriptive statistical analysis was performed for included studies. RESULTS: From 3756 citations, 91 studies met inclusion criteria. These studies described 10 unique data-collection modalities for 17 different purposes in the operating room. Data modalities included video, audio, kinematic and eye-tracking among others. Data-collection purposes described included surgical trainee assessment, surgical error, surgical team communication and operating room efficiency. CONCLUSION: Effective data collection and utilization in the operating room are imperative for the provision of superior surgical care. The future operating room landscape undoubtedly includes multiple modalities of data collection for a plethora of purposes. This review acts as a foundation for employing operating room data in a way that leads to meaningful benefit for patient care.

Necessidades pessoais de enfermeiros durante a pandemia da COVID-19 em Mato Grosso / Personal needs of nurses during the COVID-19 pandemic in Mato Grosso / Necesidades personales de enfermeras durante la pandemia da COVID-19 em Mato Grosso

Objetivo: Identificar as necessidades pessoais de Enfermeiros durante a pandemia da COVID-19 em Mato Grosso. Método: estudo exploratório e qualitativo, realizado em abril de 2020, com Enfermeiros do estado de Mato Grosso. Utilizou-se o discurso do sujeito coletivo e a Teoria das Necessidades Humanas de Maslow. Resultados: Os participantes apresentaram necessidades intermediárias (segurança e sociais) quanto as categorias da teoria de Maslow. Em relação aos discursos foram obtidas quatro ideias centrais: autocuidado, necessidade de afeto, compreensão do distanciamento e prejuízos na relação conjugal. Conclusões: As necessidades pessoais dos Enfermeiros refletem o impacto das medidas protetivas recomendadas durante a pandemia da COVID-19 tanto no contexto laboral como familiar. Descritores: Pandemias; Coronavirus; Pessoal de Saúde; Carência Psicossocial.(AU)

Objective: To identify the personal needs of Nurses during the COVID-19 pandemic in Mato Grosso. Method: exploratory and qualitative study, conducted in April 2020, with Nurses from the state of Mato Grosso. The collective subject discourse and Maslow Theory of Human Needs were used. Results: The participants presented intermediate needs (security and social) regarding the categories of Maslow theory. Regarding the speeches, four central ideas were obtained: self-care, need for affection, understanding of distance and losses in the marital relationship. Conclusions: Nurse personal needs reflect the impact of the protective measures recommended during the COVID-19 pandemic in both the work and family context. Descriptors: Pandemics; Coronavirus; Health Personnel; Psychosocial Deprivation.(AU)

Objetivo: identificar las necesidades personales de las Enfermeras durante la pandemia da COVID-19 en Mato Grosso. Metodo: estudio exploratorio y cualitativo, realizado en abril de 2020, con Enfermeras del estado de Mato Grosso. Se utilizó el discurso del sujeto colectivo y la Teoría de las Necesidades Humanas de Maslow. Resultados: Los participantes presentaron necesidades intermedias (seguridad y sociales) en relación con las categorías de la teoría de Maslow. Con respecto a los discursos, se obtuvieron cuatro ideas centrales: autocuidado, necesidad de afecto, comprensión de la distancia y pérdidas en la relación matrimonial. Conclusiones: Las necesidades personales de las Enfermeras reflejan el impacto de las medidas de protección recomendadas durante la pandemia da COVID-19, tanto en el contexto laboral como familiar. (AU)

Embedding Mobile Health Technology into the Nurses' Health Study 3 to Study Behavioral Risk Factors for Cancer.

BACKGROUND: Physical activity and sleep are behavioral risk factors for cancer that may be influenced by environmental exposures, including built and natural environments. However, many studies in this area are limited by residence-based exposure assessment and/or self-reported, time-aggregated measures of behavior. METHODS: The Nurses' Health Study 3 (NHS3) Mobile Health Substudy is a pilot study of 500 participants in the prospective NHS3 cohort who use a smartphone application and a Fitbit for seven-day periods, four times over a year, to measure minute-level location, physical activity, heart rate, and sleep. RESULTS: We have collected data on 435 participants, comprising over 6 million participant-minutes of heart rate, step, sleep, and location. Over 90% of participants had five days of ≥600 minutes of Fitbit wear-time in their first sampling week, and this percentage dropped to 70% for weeks 2 to 4. Over 819 sampling weeks, we observed an average of 7,581 minutes of heart rate and step data [interquartile range (IQR): 6,651-9,645] per participant-week, and >2 million minutes of sleep in over 5,700 sleep bouts. We have recorded location data for 5,237 unique participant-days, averaging 104 location observations per participant-day (IQR: 103-107). CONCLUSIONS: This study describes a protocol to incorporate mobile health technology into a nationwide prospective cohort to measure high-resolution objective data on environment and behavior. IMPACT: This project could provide translational insights into interventions for urban planning to optimize opportunities for physical activity and healthy sleep patterns to reduce cancer risk.See all articles in this CEBP Focus section, "Modernizing Population Science."

Improving Prediction of Fall Risk Using Electronic Health Record Data With Various Types and Sources at Multiple Times.

Inpatient falls are among the most common adverse events threatening patient safety. Although many studies have developed predictive models for fall risk, there are some drawbacks. First, most previous studies have relied on an incident-reporting system alone to identify fall events. Thus, it has been found that falls are more likely to be underreported. Second, there has been a controversy on how to select accurate representative values for patient status data across multiple times and various data sources in electronic health records. Given this background, this study used nurses' progress notes as a complementary data source to detect fall events. In addition, we developed criteria including coverage, currency, and granularity in order to integrate electronic health records data documented at multiple times in various data types and sources. Based on this methodology, we developed three models, logistic regression, Cox proportional hazard regression, and decision tree, to predict risk of patient falls and evaluate the predictive performance of these models by comparing the results to results from the Hendrich II Fall Risk Model. The findings of this study will be used in a clinical decision support system to predict risk of falling and provide evidence-based tailored recommendations in the future.

Nurses' Perceptions of Implant Barcode Scanning in Surgical Services.

The US Food and Drug Administration's 2013 Unique Device Identification System Rule requires manufacturers to label devices with unique identifiers. Implantable devices are now shipped with unique identifiers, and many electronic health records have fields to incorporate them. Health policy changes have prompted hospital systems to assess implementation of implant barcode scanning systems to capture unique device identifiers. Project aims were to assess predictors of operating room nurses' acceptance of a new implant barcode scanning system, describe operating room nurses' perceptions of the system value, and identify operating room nurses' perceived gaps in system implementation. An online survey was disseminated to operating room nurses, and focus groups were conducted with orthopedic operating room nurses in an academic medical center that had recently implemented an implant barcode scanning system in surgical services. Predictors of barcode scanning acceptance included perceived usefulness for patient care, perceived ease of use, and perceived usefulness (self). Nurses perceived the system to be more accurate and valuable for patient safety. Perceived gaps in system implementation related to communication, completeness of the system, consistency in process, and training. Understanding nurse perceptions of new barcode scanning systems and engaging them in the implementation process are key areas for success and optimization of these systems.

Superficial thermal response to CrossFit® workout

Abstract Aims: Recently, high-intensity training methods have become popular, integrating the cardiovascular and neuromuscular training in a single training session, among these methods is CrossFit®. The objective of this study was to analyze the superficial thermal response to CrossFit® exercise in men and women, in order to use this knowledge to prevent overuse injuries. Methods: Nineteen volunteers involved in CrossFit® exercise for more than 6-month (12 males and 7 females) were recruited. The acquisition of the thermal images was performed in a climatized room in two moments, at rest (before exercise), and after one CrossFit® training session. The training session lasted 45min, comprising warm-up (10-min), accessory work (15-20min), and workout of the day (15-20-min). Before the first image acquisition, volunteers were acclimated for 15 min. The Wilcoxon signed-rank test was used to compare the skin temperature between pre- and post-exercise. Results: Temperatures rose significantly pre- to post-exercise in the forearm and anterior thigh regions, while it decreased in the anterior thorax and dorsal lower back regions. These results were found both, in the overall sample, and the male volunteers, but not when the female results were isolated. Conclusion: It can be concluded that superficial thermal response to one CrossFit® training session was characterized and was different for men and women. The superficial thermal responses were aligned with the physiological alterations promoted by other modalities, such as resistance training, cycling, and running.

Planejamento organizacional no contexto de pandemia por COVID-19: implicações para a gestão em enfermagem / Organizational planning in pandemic context by COVID-19: implications for nursing management / Planificación organizativa en el contexto pandémico por COVID-19: implicaciones para la gestión de enfermería

Objetivo: refletir sobre o planejamento organizacional no contexto da pandemia por COVID-19 e as implicações para a gestão em enfermagem. Método: estudo téoricoreflexivo, realizado entre os dias 01 e 09 de junho de 2020 e baseado nos documentos emanados pela Direção Geral da Saúde de Portugal para o planejamento organizacional no contexto hospitalar, durante o período de 03 de março a 03 de junho de 2020. Resultados: foram identificados nove documentos do tipo normas ou orientações emitidas pela Direção Geral da Saúde, referentes às medidas para controle da pandemia da COVID-19, sendo possível a organização dos dados em duas macrocategorias: estruturas, materiais e procedimentos e exercício profissional dos enfermeiros, com enfoque no papel do enfermeiro gestor. Conclusão: a Direção-Geral da Saúde de Portugal, através da disseminação de documentos orientadores, permitiu um planejamento organizacional, ajustado às necessidades dos pacientes com COVID-19. O enfermeiro gestor teve de reorganizar a gestão de estruturas e materiais, assim como a gestão dos enfermeiros para cumprir as orientações emanadas e continuar a assegurar cuidados de qualidade aos pacientes internados em sua unidade.(AU)

Objective: to reflect on organizational planning in the context of the COVID-19 pandemic and the implications for nursing management. Method: theoretical-reflective study, carried out between 01 and 09 June 2020 and based on documents issued by the General Directorate of Health of Portugal for organizational planning in the hospital context, during the period from March 03 to June 03, 2020. Results: nine documents of the norms or guidelines issued by the Directorate-General for Health were identified, referring to the measures to control the pandemic of COVID-19, making it possible to organize the data in two macro categories: structures, materials and procedures and professional practice of nurses, focusing on the role of nurse manager. Conclusion: the Directorate-General for Health of Portugal, through the dissemination of guiding documents, allowed for organizational planning, adjusted to the needs of patients with COVID-19. The nurse manager had to reorganize the management of structures and materials, as well as the management of nurses to comply with the guidelines issued and continue to ensure quality care for patients admitted to his unit.(AU)

Objetivo: reflexionar sobre la planificación organizacional en el contexto de la pandemia COVID-19 y las implicaciones para el manejo de enfermería. Método: estudio teórico-reflexivo, realizado entre el 01 y el 09 de junio de 2020 y basado en documentos emitidos por la Dirección General de Salud de Portugal para la planificación organizativa en el contexto hospitalario, durante el período del 03 de marzo al 03 de junio. 2020. Resultados: se identificaron nueve documentos de las normas o directrices emitidas por la Dirección General de Salud, en relación con las medidas para controlar la pandemia de COVID-19, que permiten organizar los datos en dos macro categorías: estructuras, materiales y procedimientos y práctica profesional. de enfermeras, centrándose en el papel de gerente de enfermería. Conclusión: la Dirección General de Salud de Portugal, a través de la difusión de documentos guía, permitió la planificación organizacional, ajustada a las necesidades de los pacientes con COVID-19. El gerente de enfermería tuvo que reorganizar la gestión de estructuras y materiales, así como la gestión de las enfermeras para cumplir con las directrices emitidas y continuar garantizando una atención de calidad para los pacientes ingresados en su unidad.(AU)

Estadísticas vitales en la Ciudad de Buenos Aires / Vital statistics in the City of Buenos Aires

Informe que presenta como temas principales el origen de las estadísticas vitales a nivel nacional y provincial, la conformación vigente del sistema de estadísticas vitales en la Ciudad de Buenos Aires, recomendaciones internacionales para la recopilación de las estadísticas vitales, y el tratamiento de la calidad de las estadísticas vitales en la Ciudad de Buenos Aires. También informa sobre el relevamiento para actualizar el circuito de los informes estadísticos de nacimientos, defunciones, y defunciones fetales ocurridos en la Ciudad de Buenos Aires, realizado en seis hospitales públicos de distintas especialidades

Terapias biológicas del cáncer: un enfoque a favor de la accesibilidad / Biological cancer therapies: an approach towards accessibility

RESUMEN Los anticuerpos monoclonales son una poderosa herramienta para el diagnóstico de laboratorio y un instrumento cada vez más utilizado en el tratamiento de diversas enfermedades, siendo uno de los grupos más importantes de drogas en el tratamiento del cáncer. La revolución en el mundo de los anticuerpos ocurre en 1975 cuando Milstein y Köhler desarrollan la técnica de las hibridomas en Cambridge. Objetivo Hacer una revisión del uso de anticuerpos monoclonales en medicina y, en particular, en el tratamiento del cáncer. Se busca aportar una visión generalizada del concepto de anticuerpo monoclonal para explicar su aplicabilidad terapéutica y abordar un enfoque económico y sociosanitario de la obtención y acceso a las nuevas terapias. Método En la caracterización del fenómeno de investigación se empleó el estudio descriptivo, de recolección de datos documental y la correlación entre las distintas fuentes. Discusión Son aún elevados los costos tanto para el paciente como para los sistemas de salud pública, y se ha de optimizar la valoración costo-efectividad de modo que la rentabilidad y el acceso a tiempo para los pacientes puedan ser compatibles. Se deja abierto el reto del desarrollo de nuevos mAbs dirigidos a nuevas dianas, mejorar el perfil de seguridad, evitando o reduciendo las reacciones adversas inmunes y conseguir el abaratamiento del coste de producción mediante mejoras en la biotecnología.(AU)

ABSTRACT Monoclonal antibodies are a useful tool for laboratory diagnosis and an instrument used in the treatment of various diseases and represent one of the most important groups of new drugs for the treatment of cancer. The revolution in the world occured in 1975 when Milstein and Köhler discovered monoclonal antibodies in Cambridge. Objective To review the use of monoclonal antibodies in medicine and in the treatment of cancer. To provide a generalized vision of the concept of monoclonal antibody to explain its therapeutic applicability, and to approach an economic, health-care approach to obtaining and accessing new therapies. Method In the characterization of the research phenomenon, the descriptive study, the collection of documentary data and the correlation between the different sources were used. Discussion However, the costs for both the patient and the public health systems are still high, and the cost-effectiveness assessment must be optimized so that cost-effectiveness and access to time for patients can be compatible. And the challenge of developing new mAbs aimed at new targets, improving the safety profile, avoiding, or reducing adverse immune reactions and achieving lower production costs through improvements in biotechnology, is left open.(AU)

Challenges encountered and lessons learned during a trial of an electronic hand hygiene monitoring system.

BACKGROUND: Automated hand hygiene monitoring systems (AHHMS) are being developed to supplement direct observations of hand hygiene (HH). We compared compliance rates generated by direct observations and by a badge-based AHHMS. METHODS: Observations of HH compliance were conducted in a surgical intensive care unit (SICU) and a general medical ward (GMW) during a 7-month baseline period and a 13-week intervention period, when compliance was also estimated using an AHHMS. Compliance rates were analyzed using time-series analysis. RESULTS: During the entire pre- and postintervention period, univariate analysis of observations revealed significant improvement in overall HH compliance in the SICU (P = .001) and the GMW (P = .03), beginning before implementation of the AHHMS. Initiation of the AHHMS was associated with a transient drop in entry and exit compliance on both units. During the intervention period, observations suggested that compliance on entry and exit continued to increase in the SICU, but not in the GMW. Time series analysis of AHHMS data revealed that entry and exit compliance did not change significantly in the SICU but decreased significantly in the GMW (P = .001). The average accuracy of the AHHMS was 60%. CONCLUSIONS: Based on observations, HH compliance increased during the baseline period. Observations and the AHHMS yielded different trends in compliance. The AHHMS's accuracy of HH events and health care personnel location were suboptimal.

Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.

The ability to determine high-quality, artefact-free structures is a challenge in micro-crystallography, and the rapid onset of radiation damage and requirement for a high-brilliance X-ray beam mean that a multi-crystal approach is essential. However, the combination of crystal-to-crystal variation and X-ray-induced changes can make the formation of a final complete data set challenging; this is particularly true in the case of metalloproteins, where X-ray-induced changes occur rapidly and at the active site. An approach is described that allows the resolution, separation and structure determination of crystal polymorphs, and the tracking of radiation damage in microcrystals. Within the microcrystal population of copper nitrite reductase, two polymorphs with different unit-cell sizes were successfully separated to determine two independent structures, and an X-ray-driven change between these polymorphs was followed. This was achieved through the determination of multiple serial structures from microcrystals using a high-throughput high-speed fixed-target approach coupled with robust data processing.

Long-wavelength Mesh&Collect native SAD phasing from microcrystals.

Harnessing the anomalous signal from macromolecular crystals with volumes of less than 10 000 µm3 for native phasing requires careful experimental planning. The type of anomalous scatterers that are naturally present in the sample, such as sulfur, phosphorus and calcium, will dictate the beam energy required and determine the level of radiation sensitivity, while the crystal size will dictate the beam size and the sample-mounting technique, in turn indicating the specifications of a suitable beamline. On the EMBL beamline P13 at PETRA III, Mesh&Collect data collection from concanavalin A microcrystals with linear dimensions of ∼20 µm or less using an accordingly sized microbeam at a wavelength of 1.892 Š(6.551 keV, close to the Mn edge at 6.549 keV) increases the expected Bijvoet ratio to 2.1% from an expected 0.7% at 12.6 keV (Se K edge), thus allowing experimental phase determination using the anomalous signal from naturally present Mn2+ and Ca2+ ions. Dozens of crystals were harvested and flash-cryocooled in micro-meshes, rapidly screened for diffraction (less than a minute per loop) and then used for serial Mesh&Collect collection of about 298 partial data sets (10° of crystal rotation per sample). The partial data sets were integrated and scaled. A genetic algorithm for combining partial data sets was used to select those to be merged into a single data set. This final data set showed high completeness, high multiplicity and sufficient anomalous signal to locate the anomalous scatterers, and provided phasing information which allowed complete auto-tracing of the polypeptide chain. To allow the complete experiment to run in less than 2 h, a practically acceptable time frame, the diffractometer and detector had to run together with limited manual intervention. The combination of several cutting-edge components allowed accurate anomalous signal to be measured from small crystals.

Evaluation of Dunaliella salina growth and corresponding ß-carotene production in tubular photobioreactor / Avaliação do crescimento da Dunaliella salina e correspondente produção de ß-caroteno em fotobiorreator tubula

Microalgae, photosynthetic microorganisms, are rich in lipids, polyunsaturated fatty acids, carbohydrates, proteins, vitamins, as well as carotenoids, which are antioxidants that may protect human body from various diseases including obesity, cardiovascular disease, vision-related diseases such as macular degeneration and certain types of cancer. These natural pigments have applications in the pharmaceutical (nutraceutical), food (coloring, functional food, and supplements), and cosmetics industries (e.g. sunscreen), as well as in aquaculture (animal feed). The Dunaliella salina microalga can synthesize 10% of dry weight in ß-carotene (orange pigment, pro-vitamin A activity) under high light intensity and nitrogen and phosphorus limitation, among other stress conditions. The first chapter of this thesis presents a review focused on microalgae carotenoids: culture systems, mode of operation, and applications. In this bibliographic survey, the advantages of microalgae cultivation in relation to traditional sources (higher plants) were discussed, as well as a discussion of the main cultivation systems and their importance in cell growth. This review presented a critical analysis of the different operational regimes like batch, fed-batch, semi-continuous and continuous. Relevant information on the most important world producers of microalgae carotenoids were presented. Chapter II presents the development of a modified method of dispersive liquid-liquid microextraction (DLLME) for rapid extraction of ß-carotene from Dunaliella salina cultivated in tubular photobioreactor, with subsequent development of a rapid chromatographic screening method using a C4 column for separation of geometric isomer of ß-carotene. The use of benzene as extraction solvent and water with 50% acetone as dispersant provided the best condition for the extraction of this carotenoid. In HPLC (High Performance Liquid Chromatography), employing mobile phase composed of methanol and water (95:5, v/v), it was possible to detect/quantify ß-carotene at 14 min (retention time). Besides the short analysis time (<20 min), by the miniaturized extraction (< 10 mL organic waste) this method abide by green chemistry analytical principles. It is known that nitrogen, phosphorus, as well as carbon and vitamins are vital elements for the growth of microalgae, also determining the biochemical composition of biomass. In this sense, Chapter III presents the study of the influence of different amounts of sodium nitrate (1N = 75 mg L-1; 1.5N = 112.5 mg L-1, and 3N = 225 mg L-1) and phosphate monobasic dehydrate (1P = 5.65 mg L-1, 1.5P = 8.47 mg L-1, and 3P = 16.95 mg L-1) in seawater-based f/2 medium on the growth of Dunaliella salina and ß-carotene biosynthesis, by continuous process with different replenishment proportions (R = 20% and 80%). Best results of cell productivity were obtained by semicontinuous process (mean values of Px up to 6.7 x 104 cells mL-1 d-1 with medium 1N:1P; R =20%) in comparison with batch process cultivation. Maximum cell density (Xm) obtained in this work was not dependent of R, but the best results were obtained when using medium 1.5N:1.5P (mean values up to 5.6 x 105 cells mL-1 with R =80%) instead of 1N:1P. The content of ß-carotene in the cells, in general, was higher in cells grown in medium 1N:1P (mean yield values up to 57.5 mg g-1 with R =80%) in comparison with medium 1.5N:1.5P. The cultivation of D. salina with media 3N:3P led to a long lag phase, followed by decrease in cell density and cell lysis. The use of a tubular photobioreactor contributed to successfully cultivate this microalga without contamination by protozoa. The cultivation of Dunaliella salina in tubular photobioreactor with the use of 12:12 photoperiod was appropriate, as well as to induce carotenogenesis, in the second stage, by increasing the light intensity and absence of pH control

As microalgas, micro-organismos fotossintetizantes, são ricas em lipídios, ácidos graxos poli-insaturados, carboidratos, proteínas, vitaminas, além de carotenoides que são antioxidantes com potencial de proteger o organismo humano de várias doenças incluindo a obesidade, doenças cardiovasculares, doenças relacionadas à visão como a degeneração macular e certos tipos de câncer, entre outras. Esses pigmentos naturais têm aplicações em indústrias farmacêuticas (nutracêuticos), alimentícias (colorantes, alimentos funcionais e suplementos) e de cosméticos (exemplo: filtro solar) e na aquacultura (ração animal). A microalga Dunaliella salina é capaz de sintetizar, sob alta intensidade luminosa e limitação de nutrientes como fontes de fósforo e nitrogênio, dentre outras condições de estresse, 10 % do peso seco em ß-caroteno (pigmento laranja com atividade pró-vitamina A). Assim, neste trabalho, numa primeira etapa, foi feita uma revisão da literatura abordando a produção de carotenoides por microalgas, bem como sua aplicação. Nesse levantamento bibliográfico abordou-se, dentre outros assuntos, as vantagens do cultivo de microalgas em relação as fontes tradicionais (plantas superiores), assim como uma discussão dos diferentes sistemas de cultivos e sua importância no crescimento celular. Esse review apresentou uma análise crítica dos principais regimes operacionais como batch, fed-batch, semicontínuo e contínuo. Apresentou-se também informações relevantes sobre os mais importantes produtores mundiais de carotenoides de microalgas. Numa segunda etapa, foi desenvolvido um método modificado de microextração líquido-líquido dispersivo modificado (DLLME) para a rápida extração de ß-caroteno de Dunaliella salina cultivada em fotobiorreatores tubulares, com subsequente desenvolvimento de método cromatográfico em uma coluna C4 para a separação do isômero geométrico de ß-caroteno. A extração ótima de ß-caroteno foi obtida com benzeno como solvente extrator e água com 50% de acetona como dispersante. Empregando uma fase móvel composta por metanol e água (95:5, v/v) em HPLC, foi possível a detecção/quantificação de ß-caroteno com 14 minutos de tempo de retenção. Além dos tempos curtos de análises (<20 min), pela extração em volume reduzido (< 10 mL resíduos orgânicos) este método obedece aos princípios da química verde. Sabe-se que nitrogênio, fósforo, assim como carbono e vitaminas são elementos vitais para o crescimento das microalgas e também exercem influência na composição bioquímica da biomassa. Assim, na terceira etapa deste trabalho, estudou-se a influência das quantidades de nitrato de sódio (75 mg L-1, denominado 1N; 112,5 mg L-1, denominado 1,5N; 225 mg L-1, denominado 3N) e de fosfato monobásico dihidratado (5,65 mg L-1, denominado 1P; 8,47 mg L-1, denominado 1,5P; 16,95 mg L-1, denominado 3P) em meio f/2, que tem como base a água do mar, no crescimento e na síntese de ß-caroteno da Dunaliella salina por processo semicontínuo, com uso de frações de corte (R) de 20% e 80%. Foram obtidas produtividades celulares mais elevadas em processos semicontínuos do que em processo descontínuo, com produtividades médias de até 6,7 x 104 células mL-1 d-1 (meio 1N:1P; R =20%). A máxima concentração celular (Xm) obtida neste trabalho não foi dependente de R. Os melhores resultados de Xm foram obtidos quando se usou meio 1,5N:1,5P em vez de meio, com 1N:1P, com valores médios de até 5,6 x 105 células m L-1 (R =80%). O conteúdo de ß-caroteno nas células, de maneira geral, foi maior nas células cultivadas em meio 1N:1P do que no meio 1,5N:1,5P, com valores até 57,5 mg g-1 (R =80%). O cultivo de D. salina com o meio 3N:3P levou a uma longa fase lag, seguida por uma diminuição na concentração celular e sua lise. O cultivo de células em um fotobiorreator tubular contribuiu para um crescimento celular sem contaminação por protozoários. O cultivo de Dunaliella salina em fotobiorreator tubular com o uso de fotoperíodo 12:12 foi apropriado, assim como induzir a carotenogênese, no segundo estágio, por meio do aumento da intensidade luminosa e ausência de controle de pH

Depressão materna e ajustamento conjugal de mães jovens e sua relação com a sintomatologia psicofuncional do bebê / Maternal depression and marital adjustment of young mothers and its relationship with baby's psychofunctional symptoms

Este estudo investigou a depressão materna e a percepção de ajustamento conjugal de mães jovens, para compreender a sua repercussão sobre a sintomatologia psicofuncional do bebê. Trata-se de estudo de casos contrastantes, transversal, no qual foram entrevistadas duas mães (20 a 21 anos) porto-alegrenses, cujos bebês tinham 8 e 11 meses. Foram aplicados também o R-DAS, a EPDS e o Symptom Check List, para avaliar, respectivamente, ajustamento conjugal, depressão materna e sintomas psicofuncionais do bebê. Os dados foram integrados na análise dos casos. Os resultados apontam que a percepção de ajustamento conjugal ruim e a presença de depressão materna podem repercutir sobre a sintomatologia psicofuncional do bebê, por meio de um processo de spillover, isto é, transbordamento dos desajustes conjugais sobre a relação parental. Esses achados são relevantes para a prática clínica, na busca de intervenções psicológicas que promovam a saúde mental de casais e bebês.(AU)

This study investigated maternal depression and perception of marital adjustment of young mothers, to understand its impact on the baby's psychofunctional symptoms. It is a transversal contrasting cases study, in which two mothers (20 and 21years), whose babies were 8 and 11 months old, from Porto Alegre/RS, were interviewed. R-DAS, EPDS and Symptom Check List were also applied to evaluate, respectively, marital adjustment, maternal depression and baby's psychofunctional symptoms. Data were integrated in the case analysis. The results suggest that perception of poor marital adjustment and presence of maternal depression may contribute to baby's psychofunctional symptoms, through a spillover process, i.e., the overflow of marital maladjustment on parental relationship. These findings are relevant to clinical practice, in searching for psychological interventions that promote the mental health of couples and babies.(AU)

Indicadores emocionais de mães de bebês com risco para o desenvolvimento / Emotional indicators of Mothers of babies facing developmental risks

Este estudo analisou os indicadores de estresse parental, ansiedade e depressão de mães de bebês que apresentavam condição de risco ao desenvolvimento e relacionou-os a variáveis maternas e do bebê. Foram aplicados em 53 mães de bebês de até 24 meses os instrumentos: Índice de Stress Parental (PSI-4), Inventário de Ansiedade Traço-Estado (IDATE) e Escala Baptista de Depressão (EBADEP-A). Os resultados apontaram, no PSI, estresse em Angústia Parental em 26% das participantes. No IDATE, 40% apresentaram Ansiedade Traço e, 18% delas apresentaram indicadores de depressão. Identificou-se correlações positivas e significativas entre os indicadores emocionais avaliados com variáveis maternas e do bebê. Os achados evidenciaram a necessidade de identificar os componentes emocionais das mães de bebês que apresentavam condição de risco ao desenvolvimento para disponibilizar atendimento especializado, com ganhos para os bebês, para elas e para as suas famílias.(AU)

This study analyzed the indicators of parental stress, anxiety and depression in the mothers of infants who presented a developmental risk condition and relate them to maternal and infant variables. The instruments: Parental Stress Index (PSI-4), Trait-State Anxiety Inventory (IDATE) and Baptist Depression Scale (EBADEP-A) were applied to 53 mothers of infants up to 24 months old. The results showed, in PSI, stress in Parental Anxiety in 26% of the participants. In IDATE, 40% showed Trait Anxiety, and, 18% of them showed indicators of depression. Positive and significant correlations were identified among the emotional indicators evaluated, as well as regarding the mothers' and the babies' variables. The findings evidenced the need to identify the emotional components of the mothers of babies facing developmental risks to provide specialized care, with gains, for babies, for them and for their families.(AU)

Luto em Santa Maria: estudo da tragédia sob um olhar sistêmico / Mourning in Santa Maria: a study of the tragedy under a systemic vision

O resultado do trabalho de luto da perda de um filho depende muito de como a família está encarando essa perda e de qual o ciclo de vida pelo qual o sistema familiar está passando. A presente pesquisa aborda alguns aspectos teóricos importantes relacionados às observações feitas em dois dias de acompanhamento das atividades da Associação dos Familiares de Vítimas e Sobreviventes da Tragédia de Santa Maria (AVTSM), no município de Santa Maria, estado do Rio Grande do Sul, nos dias 11 e 12 de abril de 2017, bem como procede à análise de conteúdo de seis entrevistas realizadas com mães e pai de vítimas. O trabalho realizado pela AVTSM é de fundamental importância aos pais e às mães que participam, pois, além de proporcionar atividades psicossociais para os membros e fortalece o vínculo coletivo entre eles. Além disso, tais atividades têm caráter terapêutico e incentivam os envolvidos a melhorar e a resgatar a vontade de viver e de se adaptar à realidade cruel que é viver sem seus filhos.(AU)

The outcome from the elaboration of mourning of the loss of a son largely depends on how the family is facing this loss and what life cycle the family system is going through. The present research addresses some important theoretical aspects related to observations made in two days of monitoring from the activities of the Association of Families of Victims and Survivors of the Santa Maria's Tragedy (AVTSM), in the city of Santa Maria, in the Rio Grande do Sul State, on 11 and 12 of April in 2017. Together with the analysis of the content of six interviews made with parents of the victims. The work made by AVTSM is of fundamental importance to mothers and fathers that participate in it, because it not only proportionate psychosocial activities to the members, it also strengthens the collective bond between them. Moreover, these activities have a therapeutic character and encourages the involved to get better and recover the will to live and adapt to the cruel reality that is living without their children.(AU)