RESUMO
Background: During pregnancy, pregnant women are susceptible to malaria, contributing significantly to maternal and infant mortality. Objective: This research was conducted to study the effect of Plasmodium berghei infection in pregnant mice on fetal growth retardation through placental cell apoptosis and the change of local vascularization. Methods: Eighteen pregnant Balb/c strain mice resulting from simultanously mating were divided into two groups those were nine pregnant mice used as non infected group and nine pregnant mice infected with Plasmodium berghei on day 9th post mating used as infected group respectively. On day 15th of post mating, all of the pregnant mice were killed. Fetal weights were measured using analytic balance. Apoptosis of placental cells and VEGF expression in the placental tissue were measured using immunohistochemistry. Results: Result showed that there was sequestration of parasite-infected red blood cells (PRBCs) in intervillous space. Statistical analysis showed that the fetal weights in infected pregnant mice group was significantly lower than non infected one (p = 0.01), and the placental cell apoptosis in placental tissue of infected pregnant mice was significantly higher than the non infected one (p=0.00).There was also a significant difference on VEGF expression between infected group and non infected group (p= 0,00). Conclusion: Plasmodium berghei infection in pregnant Balb/c mice can cause fetal growth retardation due to high of placental cell apoptosis and low VEGF expression.
Assuntos
Malária , Complicações Parasitárias na Gravidez , Lactente , Gravidez , Feminino , Humanos , Camundongos , Animais , Placenta , Fator A de Crescimento do Endotélio Vascular , Retardo do Crescimento Fetal , Peso Fetal , Complicações Parasitárias na Gravidez/metabolismo , Malária/metabolismo , Camundongos Endogâmicos BALB C , ApoptoseRESUMO
BACKGROUND: Maternal malarial infection leads to poor perinatal outcomes, including low birth weight from preterm delivery and/or fetal growth restriction, particularly in primigravidas. In placental malaria, Plasmodium falciparum-infected red blood cells cause an inflammatory response that can interfere with maternal-fetal exchange, leading to poor growth. The type I interferon (IFN-I) pathway plays an immunomodulatory role in viral and bacterial infections, usually by suppressing inflammatory responses. However, its role in placental malaria is unknown. This study examines the cytokine responses in placental tissue from subsets of malaria-infected and uninfected women, and attempts to correlate them with particular birth outcomes. METHODS: 40 whole placental biopsy samples were obtained from pregnant women at least 16 years of age recruited to a larger prospective chemoprevention trial against malaria. These were patients at Tororo District Hospital in Uganda, an area of high malaria endemicity where approximately 40% of women have evidence of malaria infection at delivery. They were regularly followed at a local clinic and monitored for fever, with blood smears performed then and at time of delivery to diagnose malaria infection. Placenta biopsies were taken for histological diagnosis of placental malaria, as well as quantitative PCR analysis of genes in the IFN-I pathway (IFN-ß, IL-10 and MX-1). Parameters such as infant birth weight and gestational age were also recorded. RESULTS: Histological analysis revealed placental malaria in 18 samples, while 22 were found to be uninfected. RT-PCR analysis showed a four-fold increase in IFN-ß and IL-10 expression in multigravidas with placental malaria when compared to gravidity-matched, uninfected controls. This effect was not observed in primigravidas. Interestingly, linear regression analysis showed a positive association between IFN-ß levels and higher birth weights (ß = 101.2 g per log2-fold increase in IFN-ß expression, p = 0.042). This association was strongest in primigravidas with placental malaria (ß = 339.0, p = 0.006). CONCLUSIONS: These results demonstrate differential regulation of the IFN-I pathway in placental malaria according to gravidity, with the greatest anti-inflammatory response seen in multigravidas. The association between IFN-ß levels and higher birth weight also suggests a protective role for IFN-I against fetal growth restriction in placental malaria.
Assuntos
Peso ao Nascer/fisiologia , Número de Gestações , Interferons/metabolismo , Malária/metabolismo , Placenta/parasitologia , Complicações Parasitárias na Gravidez/metabolismo , Adolescente , Adulto , Feminino , Humanos , Malária/parasitologia , Malária/fisiopatologia , Gravidez , Complicações Parasitárias na Gravidez/parasitologia , Complicações Parasitárias na Gravidez/fisiopatologia , Uganda , Adulto JovemRESUMO
Pregnancy-associated malaria (PAM) is one of the severe forms of Plasmodium falciparum infection. The main antigen VAR2CSA is the target of vaccine development. However, the large size of VAR2CSA protein and its high degree of variability limit to the efficiency of the vaccination. Using quantitative mass spectrometry method, we detected and quantified proteotypic peptides from 5 predicted PAM associated proteins. Our results confirmed that PFI1785w is over-expressed in PAM samples. Then, we investigated PFI1785w variability among a set of parasite samples from various endemic areas. PFI1785w appear to be more conserved than VAR2CSA. PFB0115w, another PAM associated protein, seems also associated with the pathology. Further vaccination strategies could integrate other proteins in addition to the major VAR2CSA antigen to improve immune response to vaccination.
Assuntos
Antígenos de Protozoários/análise , Vacinas Antimaláricas/química , Malária Falciparum/parasitologia , Plasmodium falciparum/imunologia , Complicações Parasitárias na Gravidez/parasitologia , Antígenos de Protozoários/química , Antígenos de Protozoários/genética , Feminino , Humanos , Malária Falciparum/metabolismo , Espectrometria de Massas , Mutação , Peptídeos/química , Peptídeos/genética , Filogeografia , Gravidez , Complicações Parasitárias na Gravidez/metabolismo , Estrutura Secundária de Proteína , Proteômica , Biologia SintéticaRESUMO
Bovine neosporosis is caused by the protozoan Neospora caninum and is one of the major causes of abortion in cows. Cattle are intermediate hosts of this parasite and may have asymptomatic or symptomatic infections. Therefore, the aim of this study was to evaluate oxidative stress marker reactive oxygen species (ROS), thiobarbituric reactive acid substances (TBARS) levels, glutathione S-transferase (GST), adenosine deaminase (ADA), and butyrylcholinesterase (BChE) activities in dairy cows seropositives for N. caninum (asymptomatic or symptomatic). Dairy cows (n=90) were tested by immunofluorescent antibody assay (IFA) for N. caninum and divided accordingly into three groups: the group A (seronegatives, n=30), the group B (seropositives and asymptomatic, n=30), and the group C (seropositives and symptomatic, n=30). It was observed increased levels of TBARS and reduced (P<0.05) BChE activity in seropositives either asymptomatic or symptomatic animals. ROS levels and ADA activity increased, and GST activity decreased (P<0.05) only in seropositives symptomatic dairy cows (the group C) compared to seronegatives dairy cows (the group A). Based on these results, it was observed that seropositive animals showed cell damage associated with oxidative stress and inflammation, mainly in those with symptomatic infections. Increased seric ROS levels and BChE activity may have influenced N. caninum pathogenesis in symptomatic animals due to increased cell damage and exacerbated inflammatory response, leading to the development of clinical signs.
Assuntos
Anticorpos Antiprotozoários/sangue , Doenças dos Bovinos/metabolismo , Coccidiose/veterinária , Neospora/patogenicidade , Estresse Oxidativo , Complicações Parasitárias na Gravidez/metabolismo , Aborto Animal , Adenosina Desaminase/sangue , Animais , Butirilcolinesterase/sangue , Bovinos , Doenças dos Bovinos/parasitologia , Coccidiose/metabolismo , Coccidiose/parasitologia , Indústria de Laticínios , Feminino , Glutationa Transferase/sangue , Inflamação , Neospora/imunologia , Gravidez , Complicações Parasitárias na Gravidez/parasitologia , Espécies Reativas de Oxigênio/sangue , Substâncias Reativas com Ácido Tiobarbitúrico/análiseRESUMO
Increased TNF-α levels have been associated with adverse pregnancy outcomes. Lipopolysaccharide (LPS), 1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane (DDT), 1,1-bis-(chlorophenyl)-2,2-dichloroethene (DDE), and 1,1-dichloro-2,2-bis(chlorophenyl)ethane (DDD) induce TNF-α release in peripheral blood mononuclear cells (PBMC). Conversely, progesterone (P4) inhibits TNF-α secretion. Pregnant women in malaria endemic areas may be co-exposure to these compounds. Thus, this study was to investigate the synergistic effect of LPS and these pesticides in PBMC and to assess P4 influence on this synergy. Cultured PBMC were exposed to each pesticide in the presence of LPS, P4, or their combination. TNF-α was measured by ELISA. All pesticides enhanced TNF-α synthesis in PBMC. Co-exposure with LPS synergizes TNF-α production, which is blocked by progesterone. These results indicate that these organochlorines act synergistically with LPS to induce TNF-α secretion in PBMC. This effect is blocked by P4.
Assuntos
DDT , Leucócitos Mononucleares/metabolismo , Lipopolissacarídeos , Progesterona/farmacologia , Fator de Necrose Tumoral alfa/metabolismo , Adulto , DDT/agonistas , DDT/farmacocinética , DDT/toxicidade , Feminino , Humanos , Leucócitos Mononucleares/patologia , Lipopolissacarídeos/agonistas , Lipopolissacarídeos/toxicidade , Malária/epidemiologia , Malária/metabolismo , Malária/patologia , Gravidez , Complicações Parasitárias na Gravidez/epidemiologia , Complicações Parasitárias na Gravidez/metabolismo , Complicações Parasitárias na Gravidez/patologiaRESUMO
Adverse pregnancy outcomes place the lives of mother and new born babies in jeopardy, especially in Sub Saharan Africa. Although a well-balanced network of the pregnancy-associated hormones and lipid fractions is necessary for healthy pregnancy, the profiles of some of these biomarkers alongside those of some cytokines in relation to placental malaria (PM) and poor pregnancy outcomes are unknown. Therefore between 2013 and 2014, paired peripheral and placental blood samples were collected from 135 Cameroonian women at delivery. Parasitaemia was determined microscopically and haemoglobin levels using Coulter counter. Plasma levels of cytokines (IFN-γ, IL-1ß and IL-7) and pregnancy-associated hormones (17ß oestradiol and progesterone) were measured by ELISA and the levels of lipid fractions: total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triglycerides (TG) determined by Colorimetric enzymatic methods. Parasitaemia was inversely related to parity, haemoglobin levels and birth weight (P≤0.019). While the levels of IFN-γ and cholesterol (total, HDL and LDL) were higher in peripheral plasma, those of IL-1ß, 17ß oestradiol, progesterone and triglyceride were higher in placental blood (P<0.001). Absence of PM was significantly associated with high plasma levels of IFN-γ, IL-7 and HDL-C and low plasma levels of 17ß oestradiol and TG. Moreover, IL-7 levels correlated positively and significantly with haemoglobin levels and with both peripheral and placental levels of progesterone. Baby birth weight increased with plasma levels of progesterone and HDL-C. Levels of IFN-γ correlated positively and significantly with HDL-C, but negatively with LDL-C; thus, might prevent pregnant women from atherogenic risk. Study of the inter-relationship between hormones, cytokines and lipids revealed that the association between IL-7 and progesterone and/or some lipid fractions followed inverse trends from that of IFN-γ. These results suggest that in PM, IFN-γ and IL-7 might protect against poor pregnancy outcomes, which decrease plasma levels of progesterone, maternal haemoglobin and HDL-C, leading to low birth weight. However, these cytokines may act differently with regards to progesterone and some lipid fractions. PM may also lower plasma levels of HDL-C and increase that of TG which is the most important risk factor for cardiovascular disorders and consequently poor pregnancy outcomes.
Assuntos
Citocinas/imunologia , Malária/imunologia , Placenta/parasitologia , Complicações Parasitárias na Gravidez/imunologia , Adolescente , Adulto , Camarões , HDL-Colesterol/metabolismo , LDL-Colesterol/metabolismo , Estradiol/metabolismo , Feminino , Hemoglobinas , Humanos , Hipertrigliceridemia/metabolismo , Recém-Nascido de Baixo Peso , Recém-Nascido , Interferon gama/imunologia , Interleucina-1beta/imunologia , Interleucina-7/imunologia , Malária/metabolismo , Parasitemia/imunologia , Parasitemia/metabolismo , Paridade , Placenta/imunologia , Placenta/metabolismo , Gravidez , Complicações Parasitárias na Gravidez/metabolismo , Resultado da Gravidez , Progesterona/metabolismo , Prognóstico , Adulto JovemRESUMO
Placental malaria, especially when complicated with intervillositis, can cause fetal growth restriction. Transplacental glucose transport by glucose transporter isoform 1 (GLUT-1) on the syncytiotrophoblast microvillous and basal plasma membranes regulates fetal growth. We found that GLUT-1 expression in the microvillous plasma membrane of Plasmodium falciparum-negative placenta biopsy specimens was comparable to that in P. falciparum-positive placenta biopsy specimens with or without intervillositis, whereas GLUT-1 expression in the basal plasma membrane was lowest in P. falciparum-positive placenta biopsy specimens with intervillositis, compared with the other 2 specimen types (P ≤ .0016). GLUT-1 expression in the basal plasma membrane also correlated negatively with monocyte infiltrate density (r = -0.43; P = .003) and positively with birth weight (r = 0.28; P = .06). These findings suggest that intervillositis, more than placental malaria per se, might cause fetal growth restriction, through impaired transplacental glucose transport.
Assuntos
Retardo do Crescimento Fetal/metabolismo , Regulação da Expressão Gênica/fisiologia , Transportador de Glucose Tipo 1/metabolismo , Malária Falciparum/complicações , Placenta/metabolismo , Adolescente , Feminino , Transportador de Glucose Tipo 1/genética , Humanos , Recém-Nascido , Gravidez , Complicações Parasitárias na Gravidez/metabolismo , Adulto JovemRESUMO
INTRODUCTION: Alterations of apoptosis are commonly associated with pregnancy complications and abortion. Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors that are involved in the differential apoptosis of BeWo cells infected with distinct T. gondii strain types. METHODS: Human trophoblastic cells (BeWo cell line) were infected with RH or ME49 strains, the cytokine production was measured and the phosphorylation of anti-apoptotic ERK1/2 protein was analyzed. Also, cells were treated with different cytokines, infected with RH or ME49 strain, and analyzed for apoptosis index and Fas/CD95 death receptor expression. RESULTS: ME49-infected BeWo cells exhibited a predominantly pro-inflammatory cytokine profile, whereas cells infected with RH strain had a higher production of anti-inflammatory cytokines. Also, the incidence of apoptosis was higher in ME49-infected cells, which have been treated with pro-inflammatory cytokines compared to cells infected with RH and treated with anti-inflammatory cytokines. Moreover, Fas/CD95 expression was higher in cells infected with either ME49 or RH strain and treated with pro-inflammatory cytokines compared to anti-inflammatory cytokine treatment. The phosphorylation of ERK1/2 protein increased after 24 h of infection only with the RH strain. CONCLUSION: These results suggest that opposing mechanisms of interference in apoptosis of BeWo cells after infection with RH or ME49 strains of T. gondii can be associated with the differential cytokine profile secreted, the Fas/CD95 expression and the phosphorylated ERK1/2 expression.
Assuntos
Apoptose , Citocinas/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Placenta/parasitologia , Toxoplasma/patogenicidade , Receptor fas/metabolismo , Linhagem Celular , Citocinas/genética , Feminino , Humanos , Proteína Quinase 1 Ativada por Mitógeno/biossíntese , Proteína Quinase 3 Ativada por Mitógeno/biossíntese , Fosforilação , Placenta/imunologia , Placenta/metabolismo , Placentação , Gravidez , Complicações Parasitárias na Gravidez/imunologia , Complicações Parasitárias na Gravidez/metabolismo , Complicações Parasitárias na Gravidez/parasitologia , Complicações Parasitárias na Gravidez/patologia , Processamento de Proteína Pós-Traducional , Proteínas Recombinantes/metabolismo , Especificidade da Espécie , Toxoplasma/imunologia , Toxoplasmose/imunologia , Toxoplasmose/metabolismo , Toxoplasmose/parasitologia , Toxoplasmose/patologia , Trofoblastos/imunologia , Trofoblastos/metabolismo , Trofoblastos/parasitologia , Regulação para Cima , Virulência , Receptor fas/biossínteseRESUMO
Placental malaria (PM) can lead to poor neonatal outcomes, including low birthweight due to fetal growth restriction (FGR), especially when associated with local inflammation (intervillositis or IV). The pathogenesis of PM-associated FGR is largely unknown, but in idiopathic FGR, impaired transplacental amino acid transport, especially through the system A group of amino acid transporters, has been implicated. We hypothesized that PM-associated FGR could result from impairment of transplacental amino acid transport triggered by IV. In a cohort of Malawian women and their infants, the expression and activity of system A (measured by Naâº-dependent ¹4C-MeAIB uptake) were reduced in PM, especially when associated with IV, compared to uninfected placentas. In an in vitro model of PM with IV, placental cells exposed to monocyte/infected erythrocytes conditioned medium showed decreased system A activity. Amino acid concentrations analyzed by reversed phase ultra performance liquid chromatography in paired maternal and cord plasmas revealed specific alterations of amino acid transport by PM, especially with IV. Overall, our data suggest that the fetoplacental unit responds to PM by altering its placental amino acid transport to maintain adequate fetal growth. However, IV more profoundly compromises placental amino acid transport function, leading to FGR. Our study offers the first pathogenetic explanation for FGR in PM.
Assuntos
Aminoácidos/metabolismo , Malária Falciparum/metabolismo , Doenças Placentárias/metabolismo , Plasmodium falciparum/imunologia , Complicações Parasitárias na Gravidez/metabolismo , Adolescente , Adulto , Sistema A de Transporte de Aminoácidos/genética , Sistema A de Transporte de Aminoácidos/metabolismo , Aminoácidos/análise , Transporte Biológico , Estudos de Casos e Controles , Linhagem Celular Tumoral , Estudos de Coortes , Feminino , Retardo do Crescimento Fetal/etiologia , Retardo do Crescimento Fetal/imunologia , Retardo do Crescimento Fetal/metabolismo , Humanos , Lactente , Recém-Nascido de Baixo Peso , Recém-Nascido , Interleucina-1beta/sangue , Interleucina-1beta/metabolismo , Malária Falciparum/complicações , Malária Falciparum/imunologia , Malaui , Troca Materno-Fetal/imunologia , Monócitos , Placenta/imunologia , Placenta/metabolismo , Doenças Placentárias/imunologia , Plasmodium falciparum/fisiologia , Gravidez , Complicações Parasitárias na Gravidez/imunologia , Adulto JovemRESUMO
Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), a family of adhesins of the falciparum species of the malaria parasite, is exposed on the surface of the infected erythrocyte. In general, only one PfEMP1 variant is expressed at a time but switching between variants occurs, changing both host-cell receptor specificity and serotype. The PfEMP1 variant VAR2CSA causes sequestration of infected erythrocytes in the intervillous spaces of the placenta via the glycosaminoglycan chondroitin sulfate A. This leads to pregnancy-associated malaria, which has severe consequences for the fetus and mother. The extracellular region of VAR2CSA comprises six DBL (Duffy-binding-like) domains and a single CIDR (cysteine-rich inter-domain region) domain. The C-terminal domain DBL6ε, the most polymorphic domain of VAR2CSA, has seven regions of high variability termed variable blocks (VBs). Here we have determined the crystal structure of DBL6ε from the FCR3 parasite line and have compared it with the previously determined structure of that from the 3D7 line. We found significant differences particularly in the N-terminal region, which contains the first VB (VB1). Although DBL6ε is the most variable VAR2CSA domain, DBL6ε-FCR3 and DBL6ε-3D7 react with IgG purified from immune sera of pregnant women. Furthermore, IgG purified on one domain cross-reacts with the other, confirming the presence of cross-reactive epitopes. We also examined reactivity of immune sera to the four least variable VB (VB1, VB2, VB4 and VB5) using peptides with the consensus sequence closest, in turn, to the FCR3 or 3D7 domain. These results provide new molecular insights into immune escape by parasites expressing the VAR2CSA variant.
Assuntos
Antígenos de Protozoários/química , Malária Falciparum/imunologia , Malária Falciparum/metabolismo , Plasmodium falciparum/química , Complicações Parasitárias na Gravidez/imunologia , Proteínas de Protozoários/química , Sequência de Aminoácidos , Antígenos de Protozoários/imunologia , Cristalografia por Raios X , Feminino , Variação Genética/imunologia , Interações Hospedeiro-Parasita/imunologia , Humanos , Malária Falciparum/parasitologia , Dados de Sequência Molecular , Placenta/química , Placenta/imunologia , Placenta/parasitologia , Plasmodium falciparum/classificação , Plasmodium falciparum/imunologia , Gravidez , Complicações Parasitárias na Gravidez/metabolismo , Complicações Parasitárias na Gravidez/parasitologia , Estrutura Terciária de Proteína/genética , Proteínas de Protozoários/imunologiaRESUMO
Malarial infection during pregnancy has been associated with maternal anemia and death, abortion, still-birth and is a major cause of low birth weight, an important risk factor for infant morbidity and mortality in endemic areas. The present study was designed to delineate the oxidative stress in various organs (liver, spleen, kidney, brain and placenta) of pregnant Plasmodium berghei infected BALB/c mice. It was observed that pregnant-infected mice had higher parasitaemia than nonpregnant-infected mice. Most notably, levels of malondialdehyde (MDA), a measure of lipid peroxidation, reduced glutathione (GSH) and superoxide dismutase (SOD) levels were significantly higher in the liver, spleen, kidney and brain of pregnant-infected mice compared with pregnant mice. Although MDA levels were significantly higher, GSH and SOD levels remained unaltered in the placenta of pregnant-infected mice compared with pregnant mice. Furthermore, catalase activity was significantly lower in all the organs of pregnant-infected mice compared with pregnant mice. Histopathological observations in the organs clearly show the cellular and morphological alterations that may be occurring due to increased lipid peroxidation. Taken together, the data suggest that the increased severity of malarial infection during pregnancy may be due to accentuated oxidative stress.
Assuntos
Malária/metabolismo , Estresse Oxidativo/fisiologia , Plasmodium berghei , Complicações Parasitárias na Gravidez/metabolismo , Animais , Encéfalo/metabolismo , Encéfalo/patologia , Catalase/análise , Feminino , Glutationa/análise , Rim/metabolismo , Rim/patologia , Peroxidação de Lipídeos , Fígado/metabolismo , Fígado/patologia , Malária/patologia , Malondialdeído/análise , Camundongos , Camundongos Endogâmicos BALB C , Parasitemia/metabolismo , Parasitemia/parasitologia , Parasitemia/patologia , Placenta/metabolismo , Placenta/patologia , Gravidez , Complicações Parasitárias na Gravidez/patologia , Índice de Gravidade de Doença , Baço/metabolismo , Baço/patologia , Superóxido Dismutase/análiseRESUMO
BACKGROUND: Malaria is more common in pregnant than in non-pregnant Nigerian women, and is associated with small birth size and the attendant short- and long-term health risks. The influence of malaria on maternal metabolic status in pregnancy and in cord blood and how this relates to birth size has not been studied. The study objective was to define relationships between maternal and cord serum metabolic markers, maternal malaria status and birth size. METHODS: During pregnancy, anthropometric measurements, blood film for malaria parasites and assays for lipids, glucose, insulin and TNF were obtained from 467 mothers and these analytes and insulin-like growth factor-I (IGF-I) were obtained from cord blood of 187 babies. RESULTS: Overall prevalence of maternal malaria was 52%, associated with younger age, anaemia and smaller infant birth size. Mothers with malaria had significantly lower cholesterol (total, HDL and LDL) and higher TNF, but no difference in triglyceride. In contrast, there was no effect of maternal malaria on cord blood lipids, but the median (range) cord IGF-I was significantly lower in babies whose mothers had malaria: 60.4 (24, 145) µg/L, versus no malaria: 76.5 (24, 150) µg/L, p = 0.03. On regression analysis, the key determinants of birth weight included maternal total cholesterol, malarial status and cord insulin and IGF-I. CONCLUSIONS: Malaria in pregnancy was common and associated with reduced birth size, lower maternal lipids and higher TNF. In the setting of endemic malaria, maternal total cholesterol during pregnancy and cord blood insulin and IGF-I levels are potential biomarkers of foetal growth and birth size.
Assuntos
Biomarcadores/sangue , Sangue Fetal/química , Malária Falciparum/metabolismo , Plasmodium falciparum/fisiologia , Complicações Parasitárias na Gravidez/metabolismo , Adolescente , Adulto , Glicemia/análise , Feminino , Humanos , Recém-Nascido , Recém-Nascido Pequeno para a Idade Gestacional , Insulina/sangue , Fator de Crescimento Insulin-Like I/análise , Lipoproteínas/sangue , Malária Falciparum/parasitologia , Pessoa de Meia-Idade , Nigéria , Gravidez , Complicações Parasitárias na Gravidez/parasitologia , Fator de Necrose Tumoral alfa/sangueRESUMO
Anemia in pregnancy is a global health problem affecting nearly half of all pregnant women worldwide. High fetal demands for iron render iron deficiency the most common cause of anemia of pregnancy, with other micronutrient deficiencies contributing less frequently. In certain geographical populations, human pathogens such as hookworm, malarial parasite and human immunodeficiency virus are important factors in anemia of pregnancy. The hemoglobinopathies, sickle cell disease and thalassemia, represent diverse causes of anemia of pregnancy, requiring specialized care. Aplastic anemia is a rare, morbid cause of anemia of pregnancy and is managed with transfusions until the completion of pregnancy.
Assuntos
Anemia/etiologia , Anemia/metabolismo , Complicações Hematológicas na Gravidez/etiologia , Complicações Hematológicas na Gravidez/metabolismo , Anemia/diagnóstico , Anemia/epidemiologia , Feminino , Infecções por HIV/complicações , Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , Infecções por HIV/metabolismo , Hemoglobinas/metabolismo , Infecções por Uncinaria/complicações , Infecções por Uncinaria/diagnóstico , Infecções por Uncinaria/epidemiologia , Infecções por Uncinaria/metabolismo , Humanos , Malária/complicações , Malária/diagnóstico , Malária/epidemiologia , Malária/metabolismo , Volume Plasmático , Pré-Eclâmpsia/diagnóstico , Pré-Eclâmpsia/epidemiologia , Pré-Eclâmpsia/metabolismo , Gravidez , Complicações Hematológicas na Gravidez/diagnóstico , Complicações Hematológicas na Gravidez/epidemiologia , Complicações Neoplásicas na Gravidez/diagnóstico , Complicações Neoplásicas na Gravidez/epidemiologia , Complicações Neoplásicas na Gravidez/metabolismo , Complicações Parasitárias na Gravidez/diagnóstico , Complicações Parasitárias na Gravidez/epidemiologia , Complicações Parasitárias na Gravidez/metabolismo , Sepse/complicações , Sepse/diagnóstico , Sepse/epidemiologia , Sepse/metabolismoRESUMO
In malaria-endemic areas, iron deficiency and placental Plasmodium falciparum infection commonly coexist. In primigravidae and their newborns, hepcidin and other iron parameters were evaluated in groups and classified according to placental P. falciparum and maternal anemia status. Mothers had relatively high hepcidin levels considering their low iron status. In cord blood, levels of hepcidin, hemoglobin, and other iron parameters were also similar for groups. We conclude that maternal hepcidin is not significantly altered as a function of placental infection and/or anemia. Importantly, fetal hemoglobin and iron status were also unaffected, regardless of the presence of placental infection or maternal anemia.
Assuntos
Ferro/metabolismo , Malária Falciparum/metabolismo , Doenças Placentárias/metabolismo , Complicações Parasitárias na Gravidez/metabolismo , Adolescente , Peptídeos Catiônicos Antimicrobianos/sangue , Peptídeos Catiônicos Antimicrobianos/metabolismo , Feminino , Sangue Fetal/química , Hepcidinas , Homeostase/fisiologia , Humanos , Recém-Nascido de Baixo Peso , Recém-Nascido , Troca Materno-Fetal , Doenças Placentárias/parasitologia , Gravidez , Adulto JovemRESUMO
In Africa, approximately 25 million pregnant women are at risk of Plasmodium falciparum infection each year, one in four has evidence of placental involvement and up to half of these may be associated with low birth weight outcomes. In infected pregnant women, the placenta is an ideal site for the accumulation of the parasites, and this reduces in extent in subsequent pregnancies. Recent data indicate that terminal alpha2,3 sialic acid-dependent routes are central to the efficient invasion of erythrocytes with P. falciparum, however, the role in placental malaria of sialylated, or other glycoconjugates, on syncytiotrophoblast has not previously been assessed. Placental biopsies from Zambian women showed the Neu5Ac(alpha2,6)Gal/GalNAc sequences bound by the lectin from Sambucus nigra (SNA-1) to have greatly increased expression on microvillous membranes in samples with chronic P. falciparum infection showing, by electronic image analysis, a significant trend (p=0.002) compared to samples with past or no infection. This suggests a specific placental membrane response to falciparum malaria. Expression of alpha2,6-linked sialic acid, demonstrated by the binding of SNA-1, has been associated with intercellular repulsion in tissues from patients with cancer, and such repulsion resulting from increased alpha2,6 sialylation of chorionic villi could influence intervillous placental parasite density. Sialic acid expression should be examined in placental malaria to identify if this is a malaria-specific phenomenon, and to determine its relation to placental inflammation and pregnancy outcomes.
Assuntos
Malária Falciparum/metabolismo , Ácido N-Acetilneuramínico/metabolismo , Placenta/metabolismo , Complicações Parasitárias na Gravidez/metabolismo , Animais , Feminino , Humanos , Recém-Nascido , Estudos Longitudinais , Ácido N-Acetilneuramínico/química , Lectinas de Plantas/metabolismo , Plasmodium falciparum , Gravidez , Proteínas Inativadoras de Ribossomos/metabolismo , Regulação para CimaAssuntos
Malária/complicações , Doenças Placentárias , Complicações Parasitárias na Gravidez , Adolescente , Adulto , Feminino , Feto/metabolismo , Feto/patologia , Humanos , Malária/sangue , Malária/metabolismo , Malária/patologia , Paridade , Placenta/metabolismo , Placenta/patologia , Doenças Placentárias/sangue , Doenças Placentárias/metabolismo , Doenças Placentárias/patologia , Gravidez , Complicações Parasitárias na Gravidez/sangue , Complicações Parasitárias na Gravidez/metabolismo , Complicações Parasitárias na Gravidez/patologia , Prolactina/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
BACKGROUND: Malaria and hypertension are major causes of maternal mortality in tropical countries, especially during first pregnancies, but evidence for a relationship between these syndromes is contradictory. METHODS AND FINDINGS: In a cross-sectional survey of Tanzanian parturients, the rate of hypertension was similar in placental malaria (PM)-positive (11/85 = 13%) and PM-negative (73/602 = 12%) individuals. However, we found that PM was associated with hypertension in first-time mothers aged 18-20 y but not other mothers. Hypertension was also associated with histologic features of chronic malaria, which is common in first-time mothers. Levels of soluble vascular endothelial growth factor receptor 1 (sVEGFR1), a preeclampsia biomarker, were elevated in first-time mothers with either PM, hypertension, or both, but levels were not elevated in other mothers with these conditions. In first-time mothers with PM, the inflammatory mediator vascular endothelial growth factor (VEGF) was localized to maternal macrophages in the placenta, while sVEGFR1, its soluble inhibitor, was localized to the fetal trophoblast. CONCLUSIONS: The data suggest that maternal-fetal conflict involving the VEGF pathway occurs during PM, and that sVEGFR1 may be involved in the relationship between chronic PM and hypertension in first-time mothers. Because placental inflammation causes poor fetal outcomes, we hypothesize that fetal mechanisms that promote sVEGFR1 expression may be under selective pressure during first pregnancies in malaria-endemic areas.
Assuntos
Hipertensão/complicações , Malária/complicações , Doenças Placentárias , Complicações Cardiovasculares na Gravidez , Complicações Parasitárias na Gravidez , Adulto , Estudos Transversais , Feminino , Feto/metabolismo , Feto/patologia , Humanos , Hipertensão/sangue , Macrófagos/metabolismo , Macrófagos/patologia , Malária/sangue , Malária/metabolismo , Malária/patologia , Paridade , Placenta/metabolismo , Placenta/patologia , Doenças Placentárias/sangue , Doenças Placentárias/metabolismo , Doenças Placentárias/patologia , Gravidez , Complicações Cardiovasculares na Gravidez/sangue , Complicações Parasitárias na Gravidez/sangue , Complicações Parasitárias na Gravidez/metabolismo , Complicações Parasitárias na Gravidez/patologia , Trofoblastos/metabolismo , Trofoblastos/patologia , Fator A de Crescimento do Endotélio Vascular/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/sangueRESUMO
Materno-foetal transmission causes one of the most severe forms of infection with the protozoan parasite Toxoplasma gondii. Several studies have shown T. gondii in placental trophoblast cells, which form the barrier between maternal blood circulation and foetal tissue. Parasite multiplication in trophoblast cells is thus a critical step leading to infection of the foetus. Here, we show that multiplication of T. gondii tachyzoites was slow in BeWo trophoblast cells, compared with MRC-5 fibroblast cells. However, unlike MRC-5 cells, even combined stimulation with interferon-gamma and tumor necrosis factor-alpha did not reduce T. gondii replication in BeWo cells. This was associated with a lack of indoleamine-2,3-dioxygenase induction by these cytokines. Neither low availability of iron salts, nor an immunosuppressive action of cyclooxygenase-2 could be attributed to the low T. gondii multiplication rate in BeWo cells. However, treatment with the nitric oxide synthesis inhibitor N(G)-methyl-l-arginine and addition of ornithine enhanced the proliferation rate of the intracellular pathogen. Despite detection of inducible nitric oxide synthase-II mRNA in BeWo cells, nitric oxide production could not be detected during cell culture. Thus, inhibition of arginase activity by nitric oxide synthesis may be partially responsible for the lower multiplication rate in BeWo cells.
Assuntos
Óxido Nítrico/metabolismo , Poliaminas/metabolismo , Complicações Parasitárias na Gravidez/metabolismo , Toxoplasma/fisiologia , Toxoplasmose/transmissão , Trofoblastos/parasitologia , Animais , Arginase/metabolismo , Linhagem Celular , Inibidores de Ciclo-Oxigenase 2/farmacologia , Inibidores Enzimáticos , Feminino , Fibroblastos/parasitologia , Interações Hospedeiro-Parasita , Humanos , Indometacina/farmacologia , Transmissão Vertical de Doenças Infecciosas , Molécula 1 de Adesão Intercelular/metabolismo , Interferon gama/farmacologia , Óxido Nítrico Sintase/antagonistas & inibidores , Ornitina/farmacologia , Parasitologia/métodos , Gravidez , Reprodução , Toxoplasmose/metabolismo , Trofoblastos/metabolismo , Fator de Necrose Tumoral alfa/farmacologia , ômega-N-Metilarginina/farmacologiaRESUMO
We measured natural killer (NK) cell cytotoxicity and cortisol and prolactin concentrations in peripheral venous blood samples obtained from pregnant Gabonese women at the time of delivery. The NK cell-mediated cytotoxicity against Plasmodium falciparum-infected erythrocytes in vitro was lower in samples obtained from primiparous women than in samples obtained from multiparous women; cortisol concentrations were significantly higher in primiparous women than in multiparous women, and prolactin concentrations were significantly lower. The highest cortisol concentrations were found in the plasma of P. falciparum-infected primiparous women. A positive correlation was found between cortisol concentration and parasite load; an inverse correlation was found between the magnitude of the NK cell cytolytic effect and cortisol production. A positive correlation was found between this effect and prolactin production. Thus, depressed NK cell cytotoxicity against P. falciparum-infected erythrocytes is correlated with high cortisol concentrations and may contribute to increased susceptibility to malaria during pregnancy.
Assuntos
Eritrócitos/parasitologia , Hidrocortisona/metabolismo , Células Matadoras Naturais/imunologia , Malária Falciparum/imunologia , Plasmodium falciparum/imunologia , Complicações Parasitárias na Gravidez/imunologia , Prolactina/metabolismo , Adulto , Animais , Citotoxicidade Imunológica , Feminino , Gabão/epidemiologia , Humanos , Malária Falciparum/epidemiologia , Malária Falciparum/metabolismo , Malária Falciparum/parasitologia , Parasitemia/epidemiologia , Parasitemia/imunologia , Parasitemia/metabolismo , Gravidez , Complicações Parasitárias na Gravidez/epidemiologia , Complicações Parasitárias na Gravidez/metabolismoRESUMO
The expression of tissue factor (TF), the initiator of the clotting system, was investigated by immunohistochemical staining for its role in clotting mechanisms of Plasmodium falciparum-infected placenta. Most mononuclear cells in the intervillous space of infected placentas stained with an anti-TF monoclonal antibody (MAb) and were positive for antimacrophage MAb. The intervillous space of infected placentas had significant fibrin deposition. In contrast, only small amounts of leukocytes, TF-positive cells, and fibrin were seen in the intervillous space in noninfected placentas. These results indicate that macrophages accumulated in infected placentas express TF and that subsequent perivillous fibrin clot formation leads to a narrowing and plugging of the intervillous space and disturbance of the blood supply. Macrophage TF expression in placentas could be associated with retarded placental growth and low birth weight in malaria infection and should be further investigated.