RESUMO
OBJECTIVE: The present study aims to investigate the concentrations of Hg and its aspects methyl mercury (Me-Hg) and inorganic mercury (I-Hg) in the biological samples (BSs) of fluorescent lamp industries workers (FLIWs). METHODOLOGY: Different BSs including red blood cells (RBCs), plasma, urine, hair and nails were collected from the workers exposed to Hg and unexposed persons were selected as control group to measure both the T-Hg concentration as well as its species in different biological samples through quantitative analysis. Health data was collected through questionnaire survey. RESULTS: The mean concentrations of T-Hg (31.9 µg/L), Me-Hg (27.7 µg/L), and I-Hg (5.36 µg/L) in RBCs were found significantly ( P < 0.001) higher among the workers ( n = 40) as compared to the control group ( n = 40). Similarly the mean Hg concentrations in plasma, urine, hair and nails were also significantly higher among the workers than the control group. The statistical relation between Hg concentration and demographic characteristics observed that workers experience and fish consumption has increased the Hg concentration while age, weight and smoking found no significant effect on Hg concentration in the BSs. CONCLUSION: The study observed that the workers were highly exposed to high concentration of Hg and they are at a high health risk.
Assuntos
Monitoramento Ambiental , Utensílios Domésticos , Resíduos Industriais/análise , Compostos de Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo , Exposição Ocupacional/análise , Adulto , Humanos , Masculino , Adulto JovemRESUMO
Mercury (Hg) concentration in fish of the Gulf of the Mexico (GoM) is a major concern due to the importance of the GoM for U.S. fisheries. The Deepwater Horizon (DWH) oil spill in April 2010 in the northern GoM resulted in large amounts of oil and dispersant released to the water column, which potentially modified Hg bioaccumulation patterns in affected areas. We measured Hg species (methylmercury (MMHg) and inorganic Hg (IHg)) concentrations, and light (C, N and S) and Hg stable isotopes in muscle and liver tissues from tilefish (Lopholatilus chamaleonticeps) sampled in 2012 and 2013 along the shelf break of the northeastern GoM. Fish located close to the mouth of the Mississippi River (MR) and northwest of the DWH well-head (47â¯km) showed significantly lower Hg levels in muscle and liver than fish located further northeast of the DWH (>109â¯km), where 98% of tilefish had Hg levels in the muscle above US consumption advisory thresholds (50% for tilefish close to the DWH). Differences in light and Hg stable isotopes signatures were observed between these two areas, showing higher δ15N, and lower δ202Hg, Δ199Hg and δ34S in fish close to the DWH/MR. This suggests that suspended particles from the MR reduces Hg bioavailability at the base of the GoM food chains. This phenomenon can be locally enhanced by the DWH that resulted in increased particles in the water column as evidenced by the marine snow layer in the sediments. On the other hand, freshly deposited Hg associated with organic matter in more oligotrophic marine waters enhanced Hg bioaccumulation in local food webs. Comparing Hg isotopic composition in liver and muscle of fish indicates specific metabolic response in fish having accumulated high levels of MMHg.
Assuntos
Exposição Ambiental/análise , Compostos de Mercúrio/metabolismo , Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo , Perciformes/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , Isótopos de Carbono/análise , Monitoramento Ambiental , Golfo do México , Isótopos de Mercúrio/análise , Isótopos de Nitrogênio/análise , Isótopos de Enxofre/análiseRESUMO
INTRODUCTION: Ayurvedic and herbal medicines (AHM) are known to cause varying degrees of drug-induced liver injury (DILI). Clinical, biochemical, histological spectrum and outcomes of AHM linked to severe DILI are not well studied. METHODS: Out of 1440 liver disease patients, 94 were found to have a severe liver injury and associated AHM intake. Thirty-three patients were suspected to have AHM-DILI on Roussel Uclaf Causality Assessment Scoring Method. Forty-seven and 30 of retrieved AHM samples were analyzed for heavy metals and hepatotoxic volatile organic compounds (hVOCs), respectively. Eleven patients ingested AHM from unregistered traditional healers (UTH). Clinicopathological outcomes were analyzed in 27 patients (who underwent liver biopsy) and outcomes with respect to chemical analyses were studied in 33 patients. RESULTS: Males predominated (70.4%) with mean age 46.9±15.8 years. Mean follow up was 119.2±81.4 days. The median duration of drug intake was 28 days (10 - 84). Five patients died (18.5%). Hepatic encephalopathy, hypoalbuminemia, and hepatic necrosis were significantly associated with mortality (p < 0.005). Arsenic and mercury ingestion was significantly associated with death (p < 0.005). hVOCs were detected in more than 70% of samples. AHM intake from UTH was associated with higher mortality. CONCLUSION: Adequate regulation and scrutiny regarding AHM use among the general population is an unmet need. Early liver biopsy after clinical identification of at-risk patients can expedite definitive treatment with a liver transplant.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas/etiologia , Medicamentos de Ervas Chinesas/efeitos adversos , Medicina Herbária , Ayurveda/efeitos adversos , Adulto , Arsenicais/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/mortalidade , Doença Hepática Induzida por Substâncias e Drogas/patologia , Feminino , Seguimentos , Humanos , Fígado/patologia , Masculino , Compostos de Mercúrio/metabolismo , Pessoa de Meia-Idade , Risco , Índice de Gravidade de Doença , Fatores de Tempo , Compostos Orgânicos Voláteis/metabolismoRESUMO
Zuotai, also named as "gTso thal", a known Tibetan medicinal mixture containing insoluble cubic crystal mercuric sulfide (ß-HgS), has been used to treat diseases with long history. The mercury release ratio from Zuotai in gastrointestinal environment is one determinant factor for its bioavailability and biological effect. However, the information is still scarce now. Therefore, the study was designed to investigate the effect of sulfhydryl biomolecules [L-cysteine (Cys) and glutathione (GSH)] and pH on mercury dissociation from Zuotai, ß-HgS, and hexagonal crystal mercuric sulfide (α-HgS) in artificial gastrointestinal juices or pure water with a 1:100 solid-liquid ratio. And, the digestion and peristalsis of gastrointestinal tract were simulated in vitro. The results showed the following trend for the mercury release ratio of Zuotai, artificial gastric juice > artificial intestinal juice > pure water, whereas the trend for ß-HgS and α-HgS was as follows, artificial intestinal fluid > artificial gastric fluid > pure water. The mercury release ratios of Zuotai, ß-HgS, and α-HgS significantly increased in artificial intestinal juice containing L-Cys or GSH compared to those without sulfhydryl biomolecules in the juice. However, in contrast to the results observed for ß-HgS and α-HgS, the mercury release ratio of Zuotai was reduced remarkably in pure water and artificial gastric juice with Cys or GSH. And, we found that strong acidic or strong alkaline environments promoted the dissociation of mercury from Zuotai, ß-HgS, and α-HgS. Taken together, current findings may contribute to other studies regarding clinical safety and bioavailability of the traditional drug Zuotai containing ß-HgS.
Assuntos
Cisteína/farmacologia , Liberação Controlada de Fármacos/efeitos dos fármacos , Glutationa/farmacologia , Compostos de Mercúrio/farmacocinética , Mercúrio/farmacocinética , Disponibilidade Biológica , Suco Gástrico/química , Suco Gástrico/metabolismo , Trato Gastrointestinal/metabolismo , Concentração de Íons de Hidrogênio , Medicina Tradicional Tibetana , Mercúrio/química , Mercúrio/metabolismo , Compostos de Mercúrio/química , Compostos de Mercúrio/metabolismo , Água/química , Água/metabolismoRESUMO
Zuotai is composed mainly of ß-HgS, while cinnabar mainly contains α-HgS. Both forms of HgS are used in traditional medicines and their safety is of concern. This study aimed to compare the hepatotoxicity potential of Zuotai and α-HgS with mercury chloride (HgCl2) and methylmercury (MeHg) in mice. Mice were orally administrated with Zuotai (30 mg/kg), α-HgS (HgS, 30 mg/kg), HgCl2 (33.6 mg/kg), or CH3HgCl (3.1 mg/kg) for 7 days, and liver injury and gene expressions related to toxicity, inflammation and Nrf2 were examined. Animal body weights were decreased by HgCl2 and to a less extent by MeHg. HgCl2 and MeHg produced spotted hepatocyte swelling and inflammation, while such lesions are mild in Zuotai and HgS-treated mice. Liver Hg contents reached 45-70 ng/mg in HgCl2 and MeHg groups; but only 1-2 ng/mg in Zuotai and HgS groups. HgCl2 and MeHg increased the expression of liver injury biomarker genes metallothionein-1 (MT-1) and heme oxygenase-1 (HO-1); the inflammation biomarkers early growth response gene (Egr1), glutathione S-transferase (Gst-mu), chemokine (mKC) and microphage inflammatory protein (MIP-2), while these changes were insignificant in Zuotai and HgS groups. However, all mercury compounds were able to increase the Nrf2 pathway genes NAD(P)H: quinone oxidoreductase 1 (Nqo1) and Glutamate-cysteine ligase, catalytic subunit (Gclc). In conclusion, the Tibetan medicine Zuotai and HgS are less hepatotoxic than HgCl2 and MeHg, and differ from HgCl2 and MeHg in hepatic Hg accumulation and toxicological responses.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas/etiologia , Hepatócitos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Medicina Tradicional Tibetana , Cloreto de Mercúrio/toxicidade , Compostos de Mercúrio/toxicidade , Compostos de Metilmercúrio/toxicidade , Animais , Biomarcadores/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/genética , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Regulação da Expressão Gênica/efeitos dos fármacos , Hepatócitos/metabolismo , Hepatócitos/ultraestrutura , Mediadores da Inflamação/metabolismo , Fígado/metabolismo , Fígado/ultraestrutura , Masculino , Cloreto de Mercúrio/metabolismo , Compostos de Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo , Camundongos , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Fatores de Tempo , Redução de Peso/efeitos dos fármacosRESUMO
Secretion of inorganic mercury (Hg(2+)) from proximal tubular cells into the tubular lumen has been shown to involve the multidrug resistance-associated protein 2 (Mrp2). Considering similarities in localization and substrate specificity between Mrp2 and the breast cancer resistance protein (Bcrp), we hypothesize that Bcrp may also play a role in the proximal tubular secretion of mercuric species. In order to test this hypothesis, the uptake of Hg(2+) was examined initially using inside-out membrane vesicles containing Bcrp. The results of these studies suggest that Bcrp may be capable of transporting certain conjugates of Hg(2+). To further characterize the role of Bcrp in the handling of mercuric ions and in the induction of Hg(2+)-induced nephropathy, Sprague-Dawley and Bcrp knockout (bcrp(-/-)) rats were exposed intravenously to a non-nephrotoxic (0.5 µmol · kg(-1)), a moderately nephrotoxic (1.5 µmol · kg(-1)) or a significantly nephrotoxic (2.0 µmol · kg(-1)) dose of HgCl2. In general, the accumulation of Hg(2+) was greater in organs of bcrp(-/-) rats than in Sprague-Dawley rats, suggesting that Bcrp may play a role in the export of Hg(2+) from target cells. Within the kidney, cellular injury and necrosis was more severe in bcrp(-/-) rats than in controls. The pattern of necrosis, which was localized in the inner cortex and the outer stripe of the outer medulla, was significantly different from that observed in Mrp2-deficient animals. These findings suggest that Bcrp may be involved in the cellular export of select mercuric species and that its role in this export may differ from that of Mrp2.
Assuntos
Transportadores de Cassetes de Ligação de ATP/metabolismo , Túbulos Renais Proximais/metabolismo , Rim/metabolismo , Compostos de Mercúrio/metabolismo , Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP , Transportadores de Cassetes de Ligação de ATP/genética , Animais , Nitrogênio da Ureia Sanguínea , Creatinina/sangue , Fezes/química , Técnicas de Inativação de Genes , Rim/patologia , Túbulos Renais Proximais/citologia , Fígado/metabolismo , Masculino , Membranas/metabolismo , Cloreto de Mercúrio/metabolismo , Cloreto de Mercúrio/toxicidade , Compostos de Mercúrio/urina , Ratos , Ratos Sprague-Dawley , Proteínas de Transporte Vesicular/metabolismoRESUMO
Dissolved organic matter (DOM) is generally thought to lower metal bioavailability in aquatic systems due to the formation of metal-DOM complexes that reduce free metal ion concentrations. However, this model may not be pertinent for metal nanoparticles, which are now understood to be ubiquitous, sometimes dominant, metal species in the environment. The influence of DOM on Hg bioavailability to microorganisms was examined under conditions (0.5-5.0 nM Hg and 2-10 µM sulfide) that favor the formation of ß-HgS(s) (metacinnabar) nanoparticles. We used the methylation of stable-isotope enriched (201)HgCl(2) by Desulfovibrio desulfuricans ND132 in short-term washed cell assays as a sensitive, environmentally significant proxy for Hg uptake. Suwannee River humic acid (SRHA) and Williams Lake hydrophobic acid (WLHPoA) substantially enhanced (2- to 38-fold) the bioavailability of Hg to ND132 over a wide range of Hg/DOM ratios (9.4 pmol/mg DOM to 9.4 nmol/mg DOM), including environmentally relevant ratios. Methylmercury (MeHg) production by ND132 increased linearly with either SRHA or WLHPoA concentration, but SRHA, a terrestrially derived DOM, was far more effective at enhancing Hg-methylation than WLHPoA, an aquatic DOM dominated by autochthonous sources. No DOM-dependent enhancement in Hg methylation was observed in Hg-DOM-sulfide solutions amended with sufficient l-cysteine to prevent ß-HgS(s) formation. We hypothesize that small HgS particles, stabilized against aggregation by DOM, are bioavailable to Hg-methylating bacteria. Our laboratory experiments provide a mechanism for the positive correlations between DOC and MeHg production observed in many aquatic sediments and wetland soils.
Assuntos
Desulfovibrio desulfuricans/metabolismo , Mercúrio/metabolismo , Compostos Orgânicos/análise , Sulfetos/farmacologia , Biodegradação Ambiental/efeitos dos fármacos , Desulfovibrio desulfuricans/efeitos dos fármacos , Substâncias Húmicas/análise , Interações Hidrofóbicas e Hidrofílicas , Lagos/química , Compostos de Mercúrio/metabolismo , Metilação/efeitos dos fármacos , Compostos de Metilmercúrio/metabolismo , Modelos Químicos , Nanopartículas/química , Rios/química , Solubilidade/efeitos dos fármacosRESUMO
Mercury, one of the most toxic elements, exists in various chemical forms each with different toxicities and health implications. Some methylated mercury forms, one of which exists in fish and other seafood products, pose a potential threat, especially during embryonic and early postnatal development. Despite global concerns, little is known about the mechanisms underlying transport and toxicity of different mercury species. To investigate the impact of different mercury chemical forms on vertebrate development, we have successfully combined the zebrafish, a well-established developmental biology model system, with synchrotron-based X-ray fluorescence imaging. Our work revealed substantial differences in tissue-specific accumulation patterns of mercury in zebrafish larvae exposed to four different mercury formulations in water. Methylmercury species not only resulted in overall higher mercury burdens but also targeted different cells and tissues than their inorganic counterparts, thus revealing a significant role of speciation in cellular and molecular targeting and mercury sequestration. For methylmercury species, the highest mercury concentrations were in the eye lens epithelial cells, independent of the formulation ligand (chloride versusl-cysteine). For inorganic mercury species, in absence of l-cysteine, the olfactory epithelium and kidney accumulated the greatest amounts of mercury. However, with l-cysteine present in the treatment solution, mercuric bis-l-cysteineate species dominated the treatment, significantly decreasing uptake. Our results clearly demonstrate that the common differentiation between organic and inorganic mercury is not sufficient to determine the toxicity of various mercury species.
Assuntos
Mercúrio/metabolismo , Peixe-Zebra/metabolismo , Animais , Larva/metabolismo , Larva/ultraestrutura , Mercúrio/análise , Compostos de Mercúrio/análise , Compostos de Mercúrio/metabolismo , Compostos de Metilmercúrio/análise , Compostos de Metilmercúrio/metabolismo , Modelos Moleculares , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/metabolismo , Peixe-Zebra/anatomia & histologia , Peixe-Zebra/crescimento & desenvolvimentoRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Cinnabar (Cin), a naturally occurring mercuric sulfide (HgS), is a mineral widely used in traditional Chinese medicine throughout history. As for the toxicity of cinnabar, one important assumption is that cinnabar may be transformed into highly toxic methylmercury by gastrointestinal flora. There is no evidence in humans to support this assumption. AIM OF THE STUDY: To investigate the biotransformation of cinnabar (HgS) in the human intestinal bacteria with modern analytical techniques. MATERIALS AND METHODS: A gas chromatograph, equipped with electron capture detection (GC-ECD) and mass spectrometry (GC-MS), were used to detect the formation of methylmercury after incubation of cinnabar with human intestinal bacteria. The content of soluble mercury in the bacteria media was determined by cold vapor-atomic absorption spectrometry (CV-AAS). In addition, X-ray absorption near-edge structure spectroscopy (XANES) was used to confirm the possible transformation of cinnabar in the bacteria media, and under mimetic intestinal condition by measuring the species of sulfur and mercury in the reaction extraction of cinnabar and Na(2)S mixture. RESULTS: No methylmercury was detected by both GC-ECD and GC-MS, which suggest that cinnabar (HgS) is not methylated in the human intestine. A small amount of soluble mercury was found to be released in the flora medium of HgS or cinnabar by CV-AAS. The XANES analyses revealed that polysulfides exist in the flora medium, and the simulated results showed that the products by incubating cinnabar with Na(2)S were mercuric polysulfides. CONCLUSION: These results showed that under gut flora conditions cinnabar would be transformed into mercuric polysulfides rather than methylmercury. Our work provides evidences of nontoxic transformation of cinnabar in the human intestinal bacteria.
Assuntos
Bactérias/metabolismo , Mucosa Intestinal/metabolismo , Medicina Tradicional Chinesa , Compostos de Mercúrio/metabolismo , Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo , Sulfetos/metabolismo , Biotransformação , Humanos , Intestinos/microbiologia , Medicina Tradicional Chinesa/efeitos adversosRESUMO
Mercury toxicity is a highly interesting topic in biomedicine due to the severe endpoints and treatment limitations. Selenite serves as an antagonist of mercury toxicity, but the molecular mechanism of detoxification is not clear. Inhibition of the selenoenzyme thioredoxin reductase (TrxR) is a suggested mechanism of toxicity. Here, we demonstrated enhanced inhibition of activity by inorganic and organic mercury compounds in NADPH-reduced TrxR, consistent with binding of mercury also to the active site selenolthiol. On treatment with 5 µM selenite and NADPH, TrxR inactivated by HgCl(2) displayed almost full recovery of activity. Structural analysis indicated that mercury was complexed with TrxR, but enzyme-generated selenide removed mercury as mercury selenide, regenerating the active site selenocysteine and cysteine residues required for activity. The antagonistic effects on TrxR inhibition were extended to endogenous antioxidants, such as GSH, and clinically used exogenous chelating agents BAL, DMPS, DMSA, and α-lipoic acid. Consistent with the in vitro results, recovery of TrxR activity and cell viability by selenite was observed in HgCl(2)-treated HEK 293 cells. These results stress the role of TrxR as a target of mercurials and provide the mechanism of selenite as a detoxification agent for mercury poisoning.
Assuntos
Quelantes/farmacologia , Mercúrio/farmacologia , Selenito de Sódio/farmacologia , Tiorredoxina Dissulfeto Redutase/metabolismo , Animais , Biocatálise/efeitos dos fármacos , Domínio Catalítico , Sobrevivência Celular/efeitos dos fármacos , Quelantes/química , Cisteína/metabolismo , Relação Dose-Resposta a Droga , Ativação Enzimática/efeitos dos fármacos , Glutationa/farmacologia , Células HEK293 , Humanos , Cloreto de Mercúrio/antagonistas & inibidores , Cloreto de Mercúrio/metabolismo , Cloreto de Mercúrio/farmacologia , Mercúrio/antagonistas & inibidores , Mercúrio/metabolismo , Compostos de Mercúrio/metabolismo , Intoxicação por Mercúrio/prevenção & controle , Compostos de Metilmercúrio/antagonistas & inibidores , Compostos de Metilmercúrio/metabolismo , Compostos de Metilmercúrio/farmacologia , Estrutura Molecular , NADP/farmacologia , Ratos , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/metabolismo , Compostos de Selênio/metabolismo , Selenocisteína/metabolismo , Selenito de Sódio/metabolismo , Espectrometria de Massas por Ionização por Electrospray , Tiorredoxina Dissulfeto Redutase/antagonistas & inibidores , Tiorredoxina Dissulfeto Redutase/genéticaRESUMO
The aim of the present study was to evaluate the environmental threat to benthic species from chemical weapons dumped in the southern Adriatic Sea. An ecotoxicological approach using chemical analysis and biological responses was applied, in two sentinel species: the Blackbelly rosefish Helicolenus dactylopterus and European conger Conger conger. Specimen were collected in a stretch of sea, where had been dumped war materials and from a reference site free of ordnance. Residues of yperite, Hg and As were measured in fish fillets. Skin, liver, kidney and spleen were examined for histopathological and macroscopical lesions. Liver detoxifying capacities (EROD and UDPGT) and genotoxicity (comet assay) were also investigated. As and Hg levels were three-four times higher than those from the reference site in both species (p<0.001). Both species captured in dumping site showed clear signs of chronic illness according to the health assessment index (HAI). Deep ulcers and nodules were observed on skin and external organs. Histological lesions such as periportal and bile duct fibrosis, pericholangitis, steatosis, granuloma and elevated splenic MMCs were detected in liver and spleen. Significantly higher EROD activities were also found in both species from dumping site (p<0.01). Comet assay revealed genotoxicty in gills of C. conger from dumping site, indicating uptake of chemical warfare agents through fish gills. European conger was found to be a more sensitive bioindicator of this type of contamination than the Blackbelly rosefish.
Assuntos
Substâncias para a Guerra Química/toxicidade , Enguias/fisiologia , Peixes/fisiologia , Resíduos Industriais/efeitos adversos , Poluentes Químicos da Água/toxicidade , Animais , Arsenicais/análise , Arsenicais/metabolismo , Substâncias para a Guerra Química/análise , Substâncias para a Guerra Química/metabolismo , Cromatografia Gasosa , Ensaio Cometa , Citocromo P-450 CYP1A1/metabolismo , Dano ao DNA , Contaminação de Alimentos/análise , Brânquias/efeitos dos fármacos , Brânquias/patologia , Glucuronosiltransferase/metabolismo , Resíduos Industriais/análise , Fígado/química , Fígado/efeitos dos fármacos , Fígado/metabolismo , Mar Mediterrâneo , Compostos de Mercúrio/análise , Compostos de Mercúrio/metabolismo , Músculo Esquelético/química , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Eliminação de Resíduos , Alimentos Marinhos/análise , Pele/efeitos dos fármacos , Pele/patologia , Baço/efeitos dos fármacos , Baço/patologia , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/metabolismoRESUMO
The knowledge of vascular toxicity is important for understanding the neurotoxicity of methylmercury. In the present study, we investigated the cell-density-dependent susceptibility of human brain microvascular pericytes to methylmercury-induced toxicity by using a cell-culture system. The susceptibility of sparse pericyte cultures to methylmercury was greater than that of the dense cultures. In addition, the sparse cultures were more susceptible to methylmercury than to inorganic mercury and cadmium. The intracellular accumulation of methylmercury in the sparse cells was significantly higher than that in the dense cells. Methylmercury is transported through the L-type large neutral amino acid transporter (LAT 1) in the form of a complex with cysteine. The mRNA- and protein-level expressions of LAT 1 in the sparse cells were markedly higher than those in the dense cells; in addition, the LAT 1 expression was increased by methylmercury. However, there was no reduction in the levels of glutathione and metallothionein, which are involved in the defense mechanisms against methylmercury, in the sparse cells. The present data revealed that pericytes are markedly susceptible to methylmercury-induced cytotoxicity at low cell densities. The susceptibility of the sparse pericytes is postulated to be due to the not only constitutively higher but also methylmercury-induced expression of LAT 1, which increased the intracellular accumulation of methylmercury.
Assuntos
Circulação Cerebrovascular/efeitos dos fármacos , Compostos de Metilmercúrio/toxicidade , Pericitos/efeitos dos fármacos , Sistemas de Transporte de Aminoácidos Básicos/metabolismo , Western Blotting , Capilares/citologia , Capilares/efeitos dos fármacos , Contagem de Células , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Glutamato-Cisteína Ligase/metabolismo , Glutationa/metabolismo , Humanos , L-Lactato Desidrogenase/metabolismo , Transportador 1 de Aminoácidos Neutros Grandes/metabolismo , Compostos de Mercúrio/metabolismo , Compostos de Mercúrio/toxicidade , Metalotioneína/metabolismo , Compostos de Metilmercúrio/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Cadmium (Cd), mercury (Hg), and lead (Pb) were determined in samples of liver and breast muscles of first-year Magellanic penguins (Spheniscus magellanicus), from two different areas on the Brazilian coast, 35 on the Rio de Janeiro coast and 12 on the Rio Grande do Sul coast. In both areas, Cd concentrations in muscle samples were <0.025 microg/g. However, the Cd and Hg concentrations found in liver and Hg concentrations found in muscle showed a significant difference between the two regions. The geometric mean of the concentrations was higher in the specimens from Rio de Janeiro (Cd--6.8 microg/g; Hg--liver, 1.6 microg/g, and muscle, 0.4 microg/g wet weight) than in those from Rio Grande do Sul (Cd--2.3 microg/g; Hg--liver, 0.9 microg/g, and muscle, 0.1 microg/g wet weight). The site differences could be related to differences in diet influenced by geographic factors. Brazil's southeastern coast is highly urbanized, and its coastal waters are contaminated by the waste of agricultural and industrial activities. There is a lack of information on the levels of heavy metals in S. magellanicus, however, their wide distribution and top position in the trophic chain make the use of stranded specimens an attractive source of information for monitoring heavy metals in the South Atlantic coast.
Assuntos
Compostos de Cádmio/metabolismo , Chumbo/metabolismo , Compostos de Mercúrio/metabolismo , Spheniscidae/metabolismo , Animais , Oceano Atlântico , Brasil , Compostos de Cádmio/análise , Monitoramento Ambiental , Chumbo/análise , Fígado/química , Fígado/metabolismo , Compostos de Mercúrio/análise , Músculo Esquelético/química , Músculo Esquelético/metabolismo , Água do MarRESUMO
In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase.
Assuntos
Proteínas de Bactérias/metabolismo , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Resistência Microbiana a Medicamentos/genética , Compostos de Mercúrio/metabolismo , Compostos de Mercúrio/farmacologia , Mycobacterium marinum/efeitos dos fármacos , Mycobacterium marinum/genética , Óperon/genética , Sequência de Aminoácidos , Sequência Conservada , Primers do DNA , Escherichia coli/genética , Dados de Sequência Molecular , Plasmídeos , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
The biotransformation of Hg(II) in pH-controlled and aerated algal cultures was investigated. Previous researchers have observed losses in Hg detection in vitro with the addition of cysteine under acid reduction conditions in the presence of SnCl2. They proposed that this was the effect of Hg-thiol complexing. The present study found that cysteine-Hg, protein and nonprotein thiol chelates, and nucleoside chelates of Hg were all fully detectable under acid reduction conditions without previous digestion. Furthermore, organic (R-Hg) mercury compounds could not be detected under either the acid or alkaline reduction conditions, and only beta-HgS was detected under alkaline and not under acid SnCl2 reduction conditions. The blue-green alga Limnothrix planctonica biotransformed the bulk of Hg(II) applied as HgCl2 into a form with the analytical properties of beta-HgS. Similar results were obtained for the eukaryotic alga Selenastrum minutum. No evidence for the synthesis of organomercurials such as CH3Hg+ was obtained from analysis of either airstream or biomass samples under the aerobic conditions of the study. An analytical procedure that involved both acid and alkaline reduction was developed. It provides the first selective method for the determination of beta-HgS in biological samples. Under aerobic conditions, Hg(II) is biotransformed mainly into beta-HgS (meta-cinnabar), and this occurs in both prokaryotic and eukaryotic algae. This has important implications with respect to identification of mercury species and cycling in aquatic habitats.
Assuntos
Clorófitas/metabolismo , Cianobactérias/metabolismo , Compostos de Mercúrio/química , Mercúrio/análise , Clorófitas/crescimento & desenvolvimento , Cianobactérias/crescimento & desenvolvimento , Concentração de Íons de Hidrogênio , Cloreto de Mercúrio/química , Cloreto de Mercúrio/metabolismo , Mercúrio/química , Mercúrio/metabolismo , Compostos de Mercúrio/metabolismo , Oxirredução , Espectrofotometria Atômica , VolatilizaçãoRESUMO
Studies were carried out to characterize organomercurial lyase genes from wild type mercury-resistant Escherichia coli isolates, previously collected from five geographically distinct regions of the Indian subcontinent. PCR amplification followed by DNA sequencing of amplified fragments showed three merB identical to the previously characterized mer B from E. coli pR831b that were thus considered as the same gene. The remaining two genes derived from E. coli isolates of an almost mercury-free site (Dal lake, Kashmir) and designated as pIAAD3 merB and pIAAD14 merB showed slight variation (2%) at base. However, this variation in pIAAD3 due to the absence of base "T" at 479 position results in complete frame shift and the predicted MerB-like polypeptide derived from it showed 21.53% divergent at its C terminal end from the previously characterized pR831b MerB. The expression profile of pIAAD3 merB in pQE30 and pUC18 vectors each demonstrated 22.2 kDa proteins. The induced DH5alpha E. coli cells possessing pIAAD3 merB cloned in pUC18 vector split phenyl mercuric acetate (PMA) into benzene and inorganic mercury efficiently, thus giving a clue that the expressed gene product is biologically active. The current study suggests that such genetic changes may take place in the continued absence of mercury pressure, and with such modifications, they finally break down to act as vestigial remnants. Further work is going on in our lab to exploit pIAAD3 merB for the bioremediation of mercury-polluted sites.
Assuntos
Proteínas de Bactérias/genética , Clonagem Molecular , Farmacorresistência Bacteriana/genética , Escherichia coli/genética , Liases/genética , Compostos de Mercúrio/metabolismo , Compostos de Mercúrio/farmacologia , Sequência de Aminoácidos , Proteínas de Bactérias/química , Benzeno/metabolismo , DNA Bacteriano/análise , DNA Bacteriano/química , DNA Bacteriano/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/isolamento & purificação , Índia , Liases/química , Dados de Sequência Molecular , Peso Molecular , Compostos de Fenilmercúrio/metabolismo , Plasmídeos/genética , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
In the past two decades, a great deal of attention has been paid to the environmental fate of mercury (Hg), and this is exemplified by the growing number of international conferences devoted uniquely to Hg cycling and its impacts on ecosystem functions and life. This interest in the biogeochemistry of Hg has resulted in a significant improvement of our understanding of its impact on the environment and human health. However, both past and current research, have been primarily oriented toward the study of direct impact of anthropogenic activities on Hg cycling. Besides a few indirect effects such as the increase in Hg methylation observed in acid-rain impacted aquatic systems or the reported enhanced Hg bioaccumulation in newly flooded water reservoirs; changes in Hg transformations/fluxes that may be related to global change have received little attention. A case in point is the depletion of stratospheric ozone and the resulting increase in solar UV-radiation reaching the Earth. This review and critical discussion suggest that increasing UV-B radiation at earth's surface could have a significant and complex impact on Hg cycling including effects on Hg volatilization (photo-reduction), solubilization (photo-oxidation), methyl-Hg demethylation, and Hg methylation. Therefore, this paper is written to provoke discussions, and more importantly, to stimulate research on potential impacts of incoming solar UV-radiation on global Hg fluxes and any toxicity aspects of Hg that may become exacerbated by UV-radiation.
Assuntos
Planeta Terra , Substâncias Perigosas/metabolismo , Compostos de Mercúrio/metabolismo , Mercúrio/metabolismo , Raios Ultravioleta , Carbono/metabolismo , Carbono/efeitos da radiação , Substâncias Perigosas/toxicidade , Resíduos Perigosos/análise , Mercúrio/toxicidade , Compostos de Mercúrio/toxicidade , Oxirredução/efeitos dos fármacos , Oxirredução/efeitos da radiação , Solo/análise , Luz Solar , Volatilização/efeitos dos fármacos , Volatilização/efeitos da radiaçãoRESUMO
Feasibility of biological mercury removal from wastewater was examined by using alginate-immobilized cells of Escherichia coli carrying mer-ppk fusion plasmid pMKB18. Immobilized cells engineered to express mercury-transport system, organomercurial lyase and polyphosphate efficiently removed organic and inorganic mercury from contaminated wastewater over a wide concentration range of mercurials, probably via intracellular accumulation mediated by ppk-specified polyphosphate. Bioaccumulation of mercury was selective compared to other metals such as Cd(2+), Pb(2+) and Cr(6+). The immobilized cells could be used repeatedly (at least three times) without large loss of mercury removal activity. From these results, it is concluded that the mer-ppk fusion plasmid and the immobilized cells are useful for simultaneous removal of organic and inorganic mercury from contaminated wastewater.
Assuntos
Escherichia coli/genética , Escherichia coli/metabolismo , Compostos de Mercúrio/isolamento & purificação , Compostos Organomercúricos/isolamento & purificação , Poluentes Químicos da Água/isolamento & purificação , Fusão Gênica Artificial , Células Imobilizadas , Enterobacter aerogenes/genética , Genes Bacterianos , Compostos de Mercúrio/metabolismo , Modelos Biológicos , Compostos Organomercúricos/metabolismo , Fosfotransferases (Aceptor do Grupo Fosfato)/genética , Fosfotransferases (Aceptor do Grupo Fosfato)/metabolismo , Plasmídeos/genética , Pseudomonas/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Poluentes Químicos da Água/metabolismoRESUMO
A detailed model for the in-duct mercury capture in incinerator flue gas by powdered activated carbon injection is presented. Material balances on mercury in both gaseous and adsorbed phases are carried out along the duct length and inside the activated carbon particles, taking into account mass transfer resistances and adsorption kinetics. The set of the coupled partial differential equations is transformed by means of an orthogonal collocation technique and integrated using a Runge-Kutta method with adaptive stepsize control. The model has been applied to several sorbents of practical interest, whose parameters have been evaluated from available literature data. The values and range of the operating variables have been chosen in order to simulate typical incinerators operating conditions. Results of simulations indicate that large sorbent loadings in the duct are needed to obtain high mercury removal efficiencies, due to the short residence times. As a consequence very low utilization of the sorbents is achieved in any case. To minimize the sorbent feed rate it is particularly advisable to use a reactive sorbent and to lower the operating temperature as much as possible. Improvements in the mercury capture performance can be obtained also by increasing the in-duct particles residence time and by decreasing the sorbent particles size. Model results are compared with available relevant full scale data.
Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar/prevenção & controle , Carvão Vegetal/química , Compostos de Mercúrio/metabolismo , Adsorção , Algoritmos , Precipitação Química , Carvão Mineral , União Europeia , Gases , Incineração , Modelos Biológicos , Tamanho da Partícula , Estados Unidos , United States Environmental Protection Agency/normasRESUMO
Following a companion paper focused on the in-duct mercury capture in incinerator flue gas by powdered activated carbon injection, this paper is concerned with the additional mercury capture on the fabric filter cake, relevant to baghouse equipped facilities. A detailed model is presented for this process, based on material balances on mercury in both gaseous and adsorbed phases along the growing filter cake and inside the activated carbon particles,taking into account mass transfer resistances and adsorption kinetics. Several sorbents of practical interest have been considered, whose parameters have been evaluated from available literature data. The values and range of the operating variables have been chosen in order to simulate typical incinerators operating conditions. Results of simulations indicate that, contrary to the in-duct removal process, high mercury removal efficiencies can be obtained with moderate sorbent consumption, as a consequence of the effective gas/sorbent contacting on the filter. Satisfactory utilization of the sorbents is predicted, especially at long filtration times. The sorbent feed rate can be minimized by using a reactive sorbent and by lowering the filter temperature as much as possible. Minor benefits can be obtained also by decreasing the sorbent particle size and by increasing the cleaning cycle time of the baghouse compartments. Reverse-flow baghouses were more efficient than pulse-jet baghouses, while smoother operation can be obtained by increasing the number of baghouse compartments. Model results are compared with available relevant full scale data.