RESUMO
Background: Drug therapy for eye diseases has been limited by multiple protective mechanisms of the eye, which can be improved using well-designed drug delivery systems. Mesoporous silica nanoparticles (MSNs) had been used in many studies as carriers of therapeutic agents for ocular diseases treatment. However, no studies have focused on ocular biosafety. Considering that MSNs containing tetrasulfur bonds have unique advantages and have drawn increasing attention in drug delivery systems, it is necessary to explore the ocular biosafety of tetrasulfur bonds before their widespread application as ophthalmic drug carriers. Methods: In this study, hollow mesoporous silica nanoparticles (HMSNs) with different tetrasulfur bond contents were prepared and characterized. The ocular biosafety of HMSN-E was evaluated in vitro on the three selected ocular cell lines, including corneal epithelial cells, lens epithelial cells and retinal endothelial cells (HREC), and in vivo by using topical eye drops and intravitreal injections. Results: In cellular experiments, HMSNs caused obvious S content-dependent cytotoxic effect. HMSNs with the highest tetrasulfur bond content (HMSN-E), showed the highest cytotoxicity among all the HMSNs, and HREC was the most vulnerable cell to HMSN-E. It was shown that HMSN-E could react with intracellular GSH to generate H2S and decrease intracellular GSH concentration. Treatment of HREC with HMSN-E increased intracellular ROS, decreased mitochondrial membrane potential, and induced cell cycle arrest at the G1/S checkpoint, finally caused apoptosis and necrosis of HREC. Topical eye drops of HMSN-E could cause corneal damage. The intravitreal injection of HMSN-E could induce inflammation in the vitreum and ganglion cell layers, resulting in vitreous opacities and retinal abnormalities. Conclusion: The incorporation of tetrasulfur bonds into HMSN can have toxic effects on ocular tissues. Therefore, when mesoporous silica nanocarriers are designed for ophthalmic pharmaceuticals, the ocular toxicity of the tetrasulfur bonds should be considered.
Assuntos
Nanopartículas , Dióxido de Silício , Humanos , Animais , Nanopartículas/química , Dióxido de Silício/química , Dióxido de Silício/toxicidade , Linhagem Celular , Porosidade , Portadores de Fármacos/química , Apoptose/efeitos dos fármacos , Coelhos , Sobrevivência Celular/efeitos dos fármacos , Olho/efeitos dos fármacos , Soluções Oftálmicas/química , Soluções Oftálmicas/farmacologia , Compostos de Organossilício/química , Compostos de Organossilício/toxicidade , Espécies Reativas de Oxigênio/metabolismo , Células Epiteliais/efeitos dos fármacos , Células Endoteliais/efeitos dos fármacos , Injeções IntravítreasRESUMO
The Threshold of Toxicological Concern (TTC) for non-genotoxic substances, a risk assessment tool to establish safe exposure levels for chemicals with insufficient toxicological data, is based on the 5th percentile of cumulated distributions of Point of Departures in a high amount of repeat-dose, developmental and reproductive toxicity studies, grouped by Cramer Classes. The lack of organosilicon compounds in this dataset has resulted in regulatory concerns over the applicability of the TTC concept for this chemistry. We collected publicly available, scientifically robust oral repeat-dose and DART studies for 71 organosilicon substances for inclusion in the existing TTC dataset, using criteria for evaluation of studies and derivation of points of departure analogous to the Munro and COSMOS TTC publications. The resulting 5th percentile of this dataset was 13-fold higher than the 5th percentile for Cramer Class III compounds reported by Munro (which is the default for silicon-containing substances). Both the existing TTC for Cramer Class III compounds from Munro (1.5 µg/kg bw/day) and the COSMOS TTC (2.3 µg/kg bw/day), recommended by the SCCS for cosmetics-related substances, provide a conservative and sufficiently protective approach for this class of chemistry.
Assuntos
Compostos de Organossilício/farmacologia , Reprodução/efeitos dos fármacos , Animais , Testes de Carcinogenicidade , Cosméticos/farmacologia , Cosméticos/toxicidade , Bases de Dados Factuais , Relação Dose-Resposta a Droga , Testes de Mutagenicidade , Nível de Efeito Adverso não Observado , Compostos de Organossilício/toxicidade , Praguicidas/farmacologia , Praguicidas/toxicidade , Coelhos , RoedoresRESUMO
Chemodynamic therapy (CDT) based on the Fenton reaction is a promising strategy for nonlight cancer treatment. However, the traditional Fenton reaction is only efficient in strongly acidic conditions (pH = 2-4), resulting in the limited curative effect in a weakly acidic tumor microenvironment (TME). Herein, we first developed a simple in situ growth method to confine FeOCl nanosheets into hollow dendritic mesoporous organosilicon (H-DMOS) nanoparticles to obtain FeOCl@H-DMOS nanospheres. Ascorbic acid (AA) was then absorbed on the nanosystem as a H2O2 prodrug and, meanwhile, was used for the regeneration of Fentons reagent for Fe2+. Finally, poly(ethylene glycol) (PEG) was coated on FeOCl@H-DMOS-AA to enhance the permeability and retention (EPR) effect in tumor tissue. The as-fabricated FeOCl@H-DMOS-AA/PEG can generate a large amount of highly toxic hydroxyl radicals (â¢OH) by catalyzing H2O2 even in neutral pH conditions with the help of AA. As a result, the effect of CDT has been markedly enhanced by the increased amount of H2O2 and the efficient Fenton reaction in mild acidic TME, which can remove almost all of the tumors in mice. In addition, FeOCl also endows the nanosystem with T2-weighted MR imaging capability (r2 = 34.08 mM-1 s-1), thus realizing the imaging-guided cancer therapy. All in all, our study may contribute a new direction and may have a bright future for enhanced CDT with a neutral pH range.
Assuntos
Antineoplásicos/uso terapêutico , Meios de Contraste/uso terapêutico , Compostos de Ferro/uso terapêutico , Nanopartículas/química , Neoplasias/tratamento farmacológico , Compostos de Organossilício/química , Animais , Antineoplásicos/química , Antineoplásicos/toxicidade , Ácido Ascórbico/química , Ácido Ascórbico/uso terapêutico , Ácido Ascórbico/toxicidade , Meios de Contraste/química , Meios de Contraste/toxicidade , Feminino , Células HeLa , Humanos , Peróxido de Hidrogênio/metabolismo , Radical Hidroxila/metabolismo , Compostos de Ferro/química , Compostos de Ferro/toxicidade , Imageamento por Ressonância Magnética , Camundongos , Nanopartículas/toxicidade , Neoplasias/diagnóstico por imagem , Compostos de Organossilício/toxicidade , Polietilenoglicóis/química , Polietilenoglicóis/toxicidade , Porosidade , Pró-Fármacos/química , Pró-Fármacos/uso terapêutico , Pró-Fármacos/toxicidade , Nanomedicina Teranóstica/métodosRESUMO
Zwitterionic complexes in aqueous solutions have been extensively explored as the most promising candidate in drug delivery systems for targeted cancer chemotherapy. A POSS-based supramolecular AD-POSS-(sulfobetaine)7/CD-PLLA zwitterionic complex has been fabricated via a combination of efficient click chemistry and host-guest interaction. The well-defined POSS-based zwitterionic polymer could self-assemble into spherical nanoparticles that encapsulated a model cancer drug (DOX) and exhibited drug release in a controlled manner in a faintly acidic environment. On account of the hydrophilic block with cationic and anionic groups in the microscopic range that can form a hydration layer via electrostatic interactions, these drug-loaded nanoparticles exhibited excellent stability in a tumor intracellular microenvironment or under other pH conditions as revealed by dynamic light scattering (DLS) and zeta potential measurements. In vitro experiments demonstrated that these POSS-based nanoparticles had high resistance to non-specific protein absorption and low cytotoxicity against normal cells. Moreover, these DOX-loaded aggregates could be accumulated and effectively internalized by HeLa and MCF-7 tumor cells, exhibiting effective cellular proliferation inhibition via the release of anticancer agents. Therefore, these POSS-based supramolecular amphiphilic zwitterionic complexes, relying on the simple supramolecular interaction and efficient click reaction, could further emerge as a potential universal anticancer drug nanocarrier system for multifunctional cancer chemotherapy.
Assuntos
Portadores de Fármacos/química , Interações Hidrofóbicas e Hidrofílicas , Compostos de Organossilício/química , Células 3T3 , Absorção Fisico-Química , Animais , Transporte Biológico , Bovinos , Proliferação de Células/efeitos dos fármacos , Doxorrubicina/química , Portadores de Fármacos/metabolismo , Portadores de Fármacos/toxicidade , Liberação Controlada de Fármacos , Humanos , Concentração de Íons de Hidrogênio , Células MCF-7 , Teste de Materiais , Camundongos , Nanopartículas/química , Compostos de Organossilício/metabolismo , Compostos de Organossilício/toxicidade , Poliésteres/química , Soroalbumina Bovina/químicaRESUMO
Near-infrared photoimmunotherapy (NIR-PIT) is a new cancer phototherapy modality using an antibody conjugated to a photosensitizer, IRDye700DX. When the conjugate binds to the plasma membrane and is exposed to NIR light, NIR-PIT-treated cells undergo swelling, and target-selective necrotic/immunogenic cell death is induced. However, the cytotoxic mechanism of NIR-PIT has not been elucidated. In order to understand the mechanism, it is important to elucidate how the damage to the plasma membrane induced by NIR light irradiation changes over time. Thus, in the present study, we investigated the changes in plasma membrane permeability using ions and molecules of various sizes. Na+ flowed into cells immediately after NIR light irradiation, even when the function of transporters or channels was blocked. Subsequently, fluorescent molecules larger than Na+ entered the cells, but the damage was not large enough for dextran to pass through at early time points. To assess these phenomena quantitatively, membrane permeability was estimated using radiolabeled ions and molecules: 111 InCl3 , 111 In-DTPA, and 3 H-H2 O, and comparable results were obtained. Although minute plasma membrane perforations usually do not induce cell death, our results suggest that the minute damage induced by NIR-PIT was irreversibly extended with time. In conclusion, minute plasma membrane damage is a trigger for the increase in plasma membrane permeability, cell swelling, and necrotic/immunogenic cell death in NIR-PIT. Our findings provide new insight into the cytotoxic mechanism of NIR-PIT.
Assuntos
Permeabilidade da Membrana Celular/efeitos dos fármacos , Membrana Celular/patologia , Imunoterapia/efeitos adversos , Indóis/toxicidade , Transporte de Íons/efeitos dos fármacos , Compostos de Organossilício/toxicidade , Fototerapia/efeitos adversos , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Humanos , Imunoterapia/métodos , Indóis/uso terapêutico , Compostos de Organossilício/uso terapêutico , Fototerapia/métodos , Sódio/metabolismo , Trastuzumab/uso terapêuticoRESUMO
Dimethiconol and its esters and reaction products are used in cosmetics as either skin-conditioning agents or hair-conditioning agents. The Cosmetic Ingredient Review (CIR) Expert Panel reviewed relevant data and concluded that these ingredients are safe in the present practices of use and concentration described in this safety assessment. While there is an absence of data on reproductive and developmental toxicity and limited tumorigenicity and toxicokinetics data, the Panel reasoned that these ingredients would not be absorbed through the skin, obviating concern over potential reproductive and developmental toxicity or carcinogenicity.
Assuntos
Qualidade de Produtos para o Consumidor , Cosméticos/química , Cosméticos/toxicidade , Compostos de Organossilício/toxicidade , Testes de Toxicidade/métodos , Animais , Ésteres , Humanos , Dose Letal Mediana , Estrutura Molecular , Compostos de Organossilício/químicaRESUMO
Sandwich-like molybdenum sulfide/mesoporous organosilica nanosheets (denoted as MoS2@MOS) have been prepared for the first time via direct growth of ethane-bridged mesostructured organosilica on MoS2 nanosheets by using cetyltrimethylammonium bromide (CTAB) as structure directing agent. The obtained MoS2@MOS nanosheets possess well-defined sandwich-like structure, high surface area (â¼920cm2/g), uniform pore size (â¼4.2nm), large pore volume (â¼1.41cm3g-1). In vitro cytotoxicity assessments demonstrate that the MoS2@MOS nanosheets have excellent biocompatibility. Owing to the encapsulation of the MoS2, the obtained MoS2@MOS nanosheets have photo-thermal conversion capability and photo-thermally controlled drug release property. These properties make the MoS2@MOS nanosheets promising for biomedical applications.
Assuntos
Dissulfetos/química , Molibdênio/química , Nanoestruturas/química , Compostos de Organossilício/química , Antineoplásicos/química , Antineoplásicos/farmacologia , Materiais Biocompatíveis/química , Sobrevivência Celular/efeitos dos fármacos , Cetrimônio , Compostos de Cetrimônio/química , Dissulfetos/toxicidade , Doxorrubicina/química , Doxorrubicina/farmacologia , Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Temperatura Alta , Humanos , Lasers , Células MCF-7 , Molibdênio/toxicidade , Nanoestruturas/efeitos da radiação , Nanoestruturas/toxicidade , Compostos de Organossilício/toxicidade , Tamanho da Partícula , Porosidade , Propriedades de SuperfícieRESUMO
The toxicological effects of nanoparticles (NPs) on humans, animals, and environment are largely unknown. Assessment of NPs cytotoxicity depends on the choice of the test system. Due to NPs optical activity and absorption values, they can influence the classical cytotoxicity assay. Eight NPs were spiked in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet assays and tested with HaCaT human skin cells. The MTT assay standard curve optical density (OD) measurements were altered by the presence of trisilanol phenyl and trisilanol isooctyl polyhedral oligomeric silsesquioxane particles. The crystal violet standard curve OD measurements were significantly shifted by gold NPs, but they did not affect the MTT assay. Carbon black decreased ODs in the MTT and crystal violet assays and was localized in the cell cytoplasm. These findings strongly indicate that a careful choice of in vitro viability systems is required to avoid flawed measurement of NPs toxicity.
Assuntos
Bioensaio/métodos , Violeta Genciana , Nanopartículas/toxicidade , Sais de Tetrazólio , Tiazóis , Testes de Toxicidade/métodos , Compostos de Cádmio/toxicidade , Linhagem Celular , Ouro/toxicidade , Humanos , Compostos de Organossilício/toxicidade , Dióxido de Silício/toxicidade , Fuligem/toxicidade , Sulfetos/toxicidadeRESUMO
Cancer photodynamic therapy (PDT) by photosensitizers (PS)-loaded polymeric micelles (PM) is hampered by the tendency of PS to aggregate in PM and/or by premature release of PS in the blood circulation. In the present study, aromatic thermosensitive PM, characterized by π-π stacking interaction, are used to encapsulate an axially solketal-substituted silicon phthalocyanine (Si(sol)2 Pc) with enhanced loading capacity, smaller size, and significantly improved retention of Si(sol)2 Pc compared with systems based on thermosensitive PM lacking aromatic groups. Interestingly, Si(sol)2 Pc is much less prone to aggregation in the aromatic PM, i.e., the amount of Si(sol)2 Pc that could be encapsulated without aggregation is 330 times higher in the aromatic PM than in the nonaromatic PM. Furthermore, Si(sol)2 Pc in the aromatic PM in a molecularly dissolved (non-aggregated) form displays three times more efficient singlet oxygen production than Si(sol)2 Pc aggregated in the non-aromatic PM. As a result, the photocytotoxicity of Si(sol)2 Pc-loaded aromatic PM to B16F10 cells is increased, compared with that of the non-aromatic PM, while no significant cytotoxicity is observed in the dark. Fluorescence-activated cell sorting (FACS) and confocal laser scanning microscopy (CLSM) analysis shows cell uptake of Si(sol)2 Pc loaded in the aromatic PM, and the Si(sol)2 Pc is taken up by the cells together with the micelles. The efficient singlet oxygen production of Si(sol)2 Pc dissolved in the aromatic PM makes it an interesting formulation for cancer PDT.
Assuntos
Micelas , Fármacos Fotossensibilizantes/química , Polietilenoglicóis/química , Oxigênio Singlete/metabolismo , Animais , Antineoplásicos/química , Antineoplásicos/toxicidade , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Citometria de Fluxo , Indóis/química , Indóis/toxicidade , Camundongos , Compostos de Organossilício/química , Compostos de Organossilício/toxicidade , Fármacos Fotossensibilizantes/toxicidade , TemperaturaRESUMO
Aggressive forms of adult T-cell leukemia (ATL) respond poorly to conventional anticancer chemotherapy, and new lead compounds are required for the development of drugs to treat this fatal disease. Recently, we developed ATL cell-selective proliferation inhibitors based on a tetrahydrotetramethylnaphthalene (TMN) skeleton 1, and here we report the design and synthesis of silicon analogs of TMN derivatives. Among them, compound 13 showed the most potent growth-inhibitory activity towards the ATL cell line S1T, though its selectivity for S1T over the non-ATL cell line MOLT-4 was only moderate. This result, as well as computational studies, suggests that sila-substitution (C/Si exchange) is useful for structure optimization of these inhibitors.
Assuntos
Antineoplásicos/química , Compostos de Organossilício/química , Antineoplásicos/síntese química , Antineoplásicos/toxicidade , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Humanos , Leucemia de Células T/tratamento farmacológico , Naftalenos/química , Compostos de Organossilício/síntese química , Compostos de Organossilício/toxicidade , Eletricidade EstáticaRESUMO
Quantum dots (QDs) are fluorescent semiconductor nanocrystals that have the potential for major advancements in the field of nanomedicine through their unique photophysical properties. They can potentially be used as fluorescent probes for various biomedical imaging applications, including cancer localization, detection of micrometastasis, image guided surgery, and targeted drug delivery. Their main limitation is toxicity, which requires a biologically compatible surface coating to shield the toxic core from the surrounding environment. However, this leads to an increase in QD size that may lead to problems of excretion and systemic sequestration. We describe a one pot synthesis, characterization, and in vitro cytotoxicity of a novel polyhedral oligomeric silsesquioxane (POSS)-coated CdTe-cored QD using mercaptosuccinic acid (MSA) and D-cysteine as stabilizing agents. Characterization was performed using transmission electron microscopy Fourier transform infrared spectroscopy, and photoluminescence studies. POSS-coated QDs demonstrated high colloidal stability and enhanced photostability on high degrees of ultraviolet (UV) excitation compared to QDs coated with MSA and D-cysteine alone (P value < 0.05). In vitro toxicity studies showed that both POSS and MSA-QDs were significantly less toxic than ionized salts of Cd(+2) and Te(-2). Confocal microscopy confirmed high brightness of POSS-QDs in cells at both 1 and 24 hours, indicating that these QDs are rapidly taken up by cells and remain photostable in a biological environment. We therefore conclude that a POSS coating confers biological compatibility, photostability, and colloidal stability while retaining the small size and unique photophysical properties of the QDs. The amphiphilic nature of the coating allows solubility in aqueous solutions and rapid transfer across cell membranes, enabling the use of lower concentrations of the QDs for an overall reduced toxicity particularly for prolonged live cell and in vivo imaging applications.
Assuntos
Compostos de Organossilício/química , Compostos de Organossilício/toxicidade , Pontos Quânticos , Análise de Variância , Compostos de Cádmio/química , Forma Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cisteína/química , Células Hep G2 , Humanos , Microscopia Confocal , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície , Telúrio/química , Tiomalatos/química , Tiomalatos/toxicidade , Raios UltravioletaRESUMO
The application of photosensitisers (PSs) in photodynamic therapy (PDT) is often hampered by their hydrophobicity, as this complicates their formulation and results in an unfavourable biodistribution. Consequently, there is an urgent need for novel delivery vehicles for PSs. In this paper, the loading and stability of thermosensitive mPEG-b-p(HPMAm-Lac2) micelles with a hydrophobic solketal-substituted phthalocyanine (Si(sol)2Pc) photosensitiser were studied. It was shown that the Si(sol)2Pc could be loaded efficiently in the micelles (diameter 75 nm) up to a concentration of approximately 2 mg/mL. UV/Vis and fluorescence spectroscopy showed that at low concentrations (< or =0.05 microM, 0.45 mg/mL polymer), the PS was molecularly dissolved in the micellar core, whereas it was present in an aggregated form at higher concentrations. In B16F10 and 14C cells, the photocytotoxicity of Si(sol)2Pc-loaded micelles (PS<0.05 microM) was similar to free PS, i.e. IC(50) of 3.0+/-0.2 nM (10% serum). The cellular uptake of high-loaded micelles (10 microM Si(sol)2Pc) was low and independent of the serum concentration. The nanoaggregates of Si(sol)2Pc loaded in the micellar core were only released upon hydrolysis-induced micellar dissociation, which was observed after 5.5 h at pH 8.7 at 37 degrees C. The stability of the high-loaded micellar Si(sol)2Pc formulation also in the presence of serum, the controlled release of the PS upon micellar disintegration and the high photodynamic activity of Si(sol)2Pc make these micelles interesting for future in vivo studies.
Assuntos
Micelas , Compostos Organometálicos/síntese química , Compostos de Organossilício/síntese química , Fármacos Fotossensibilizantes/síntese química , Animais , Carcinoma de Células Escamosas/tratamento farmacológico , Carcinoma de Células Escamosas/patologia , Linhagem Celular Tumoral , Estabilidade de Medicamentos , Neoplasias de Cabeça e Pescoço/tratamento farmacológico , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Melanoma Experimental/tratamento farmacológico , Melanoma Experimental/patologia , Camundongos , Microscopia Eletrônica , Peso Molecular , Compostos Organometálicos/química , Compostos Organometálicos/metabolismo , Compostos Organometálicos/toxicidade , Compostos de Organossilício/química , Compostos de Organossilício/metabolismo , Compostos de Organossilício/toxicidade , Tamanho da Partícula , Fotoquimioterapia , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/metabolismo , Fármacos Fotossensibilizantes/toxicidade , Polietilenoglicóis/química , Polímeros/química , Espectrofotometria Ultravioleta , Temperatura , Fatores de TempoRESUMO
Macromolecules with defined nanosizes--nanoglobules--were synthesized and characterized as novel drug carriers with precise molecular architectures. Poly-L-lysine dendrimers with a cubic octa(3-aminopropyl)silsesquioxane (OAS) core, (L-lysine 8-OAS, (L-lysine) 16-(L-lysine) 8-OAS, (L-lysine) 32-(L-lysine) 16-(L-lysine) 8-OAS, and (L-lysine) 64-(L-lysine) 32-(L-lysine) 16-(L-lysine) 8-OAS, were divergently synthesized by solution phase peptide chemistry in good yield and purity. Matrix-assisted laser desorption time of flight (MALDI-TOF) mass spectrometry showed complete substitution of the surface amino groups of lower generation dendrimers during synthesis, as well as precisely defined molecular architectures of the nanoglobules. The structures of the nanoglobules were further characterized by (1)H- and (13)C-NMR and 2D-NMR (correlation spectroscopy (COSY) and pulsed-field-gradient heteronuclear multiple quantum correlation (gHMQC)) spectroscopy. The (1)H-NMR spectroscopy revealed that the nanoglobules had a relatively rigid molecular architecture. Cytotoxicity studies showed that these nanoglobules exhibited a size-dependent toxicity, but it was much lower than that of linear poly-L-lysine. Preliminary in vitro nucleic acid delivery studies have shown that these globular dendrimers can efficiently deliver plasmid DNA to MDA-MB-231 cells. These nanoglobules hold much promise as safe drug carriers with precisely defined molecular architecture.
Assuntos
Dendrímeros/síntese química , Portadores de Fármacos/química , Nanopartículas/química , Compostos de Organossilício/síntese química , Polilisina/química , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Dendrímeros/química , Dendrímeros/toxicidade , Técnicas de Transferência de Genes , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Compostos de Organossilício/química , Compostos de Organossilício/toxicidadeRESUMO
In this paper, we have investigated the ability to sensitize the phototoxicity toward HeLa cells in vitro, of tetra-tert-butylphthalocyaninatosilicon (SiPc) covalently linked to one or two 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) radicals (R1c or R2c), which are shown as photosensitizers efficiently producing singlet oxygen (1Delta(g)). Addition of R1c or R2c encapsulated in liposomes to cultures, followed by irradiation with a 680-nm dye laser, resulted in a highly significant phototoxicity toward HeLa cells, in contrast to negligible phototoxicity observed with (dihydroxy)SiPc (R0). EPR measurements indicate that R1c and R2c exist in some degree as nitroxide radicals even in HeLa cells. Electronic absorption spectra indicate that the degree of aggregation increases in the order R2cAssuntos
Antineoplásicos/toxicidade
, Indóis/toxicidade
, Compostos de Organossilício/toxicidade
, Fármacos Fotossensibilizantes/toxicidade
, Antineoplásicos/química
, Óxidos N-Cíclicos/química
, Células HeLa
, Humanos
, Indóis/química
, Compostos de Organossilício/química
, Fotoquímica
, Fármacos Fotossensibilizantes/química
RESUMO
The tumor-promoting activities of 5 commercial compounds used in termiticides were measured by a cell-transformation assay employing Bhas 42 cells. Their initiating activities were also measured by the microsuspension assay employing S. typhimurium TA98 and TA100 strains. The results of the transformation assay confirmed the tumor-promoting activities of fenitrothion, silafluofen and bifenthrin. Furthermore, the mutagenicity of S-421 and fenitrothion were also confirmed. Consideration of 2-stage carcinogenesis suggests that concurrent use of and long-term exposure to these compounds that have tumor-promoting and initiator activity, and compounds exhibiting either type of activity individually should be avoided as much as possible.
Assuntos
Transformação Celular Neoplásica/induzido quimicamente , Inseticidas/farmacocinética , Inseticidas/toxicidade , Biotransformação , Éteres/farmacocinética , Éteres/toxicidade , Fenitrotion/farmacologia , Fenitrotion/toxicidade , Testes de Mutagenicidade , Compostos de Organossilício/farmacocinética , Compostos de Organossilício/toxicidade , Permetrina/farmacocinética , Permetrina/toxicidade , Piretrinas/farmacocinética , Piretrinas/toxicidade , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/genéticaRESUMO
Photodynamic therapy (PDT) is a promising therapeutic modality that utilizes a combination of a photosensitizer and visible light for the destruction of diseased tissues. Using human-pigmented melanoma cells, we examined the photokilling efficacy of new silicon-phthalocyanines (SiPc) that bore bulky axial substituents. The bis(cholesteryloxy) derivate (Chol-O-SiPc) displayed the best in vitro photokilling efficacy (LD(50) = 6-8 x 10(-9) M) and was seven to nine times more potent than chloro-aluminium Pc (ClAlPc), a known photosensitizer used as a reference. Although Chol-O-SiPc was half as potent as ClAlPc for promoting photo-oxidative membrane damage in a cell-free assay, early events of mitochondrion-mediated apoptosis upon PDT were triggered much faster, as demonstrated by kinetics studies examining cells with permeabilized mitochondrial membranes, cytochrome c release and caspase-9 activation. Inhibition of caspase-9 activity by a substrate analogue argued for its central role in the proapoptotic events leading to cell death by Chol-O-SiPc PDT. In addition, immunoblots showed that Bcl-2 antiapoptotic oncoprotein was not a primary target of Chol-O-SiPc in M3Dau cells treated with PDT. Conclusively, Chol-O-SiPc is a useful new photosensitizer with the property of triggering cell apoptosis mediated by mitochondria.
Assuntos
Apoptose/efeitos dos fármacos , Indóis/toxicidade , Melanoma/patologia , Mitocôndrias/patologia , Compostos de Organossilício/toxicidade , Caspase 9 , Caspases/metabolismo , Linhagem Celular Tumoral , Permeabilidade da Membrana Celular , Humanos , Mitocôndrias/efeitos dos fármacos , Fotoquimioterapia , Fármacos Fotossensibilizantes/toxicidade , Proteínas Proto-Oncogênicas c-bcl-2/metabolismoRESUMO
Dendrimers are highly branched macromolecules of low polydispersity that provide many exciting opportunities for design of novel drug-carriers, gene delivery systems and imaging agents. They hold promise in tissue targeting applications, controlled drug release and moreover, their interesting nanoscopic architecture might allow easier passage across biological barriers by transcytosis. However, from the vast array of structures currently emerging from synthetic chemistry it is essential to design molecules that have real potential for in vivo biological use. Here, polyamidoamine (PAMAM, Starburst), poly(propyleneimine) with either diaminobutane or diaminoethane as core, and poly(ethylene oxide) (PEO) grafted carbosilane (CSi-PEO) dendrimers were used to study systematically the effect of dendrimer generation and surface functionality on biological properties in vitro. Generally, dendrimers bearing -NH(2) termini displayed concentration- and in the case of PAMAM dendrimers generation-dependent haemolysis, and changes in red cell morphology were observed after 1 h even at low concentrations (10 microg/ml). At concentrations below 1 mg/ml CSi-PEO dendrimers and those dendrimers with carboxylate (COONa) terminal groups were neither haemolytic nor cytotoxic towards a panel of cell lines in vitro. In general, cationic dendrimers were cytotoxic (72 h incubation), displaying IC(50) values=50-300 microg/ml dependent on dendrimer-type, cell-type and generation. Preliminary studies with polyether dendrimers prepared by the convergent route showed that dendrimers with carboxylate and malonate surfaces were not haemolytic at 1 h, but after 24 h, unlike anionic PAMAM dendrimers they were lytic. Cationic 125I-labelled PAMAM dendrimers (gen 3 and 4) administered intravenously (i.v.) to Wistar rats ( approximately 10 microg/ml) were cleared rapidly from the circulation (<2% recovered dose in blood at 1 h). Anionic PAMAM dendrimers (gen 2.5, 3.5 and 5.5) showed longer circulation times ( approximately 20-40% recovered dose in blood at 1 h) with generation-dependent clearance rates; lower generations circulated longer. For both anionic and cationic species blood levels at 1 h correlated with the extent of liver capture observed (30-90% recovered dose at 1 h). 125I-Labelled PAMAM dendrimers injected intraperitoneally were transferred to the bloodstream within an hour and their subsequent biodistribution mirrored that seen following i.v. injection. Inherent toxicity would suggest it unlikely that higher generation cationic dendrimers will be suitable for parenteral administration, especially if they are to be used at a high dose. In addition it is clear that dendrimer structure must also be carefully tailored to avoid rapid hepatic uptake if targeting elsewhere (e.g. tumour targeting) is a primary objective.
Assuntos
Sistemas de Liberação de Medicamentos , Poliaminas/química , Animais , Materiais Biocompatíveis , Sobrevivência Celular/efeitos dos fármacos , Dendrímeros , Portadores de Fármacos , Hemólise/efeitos dos fármacos , Indicadores e Reagentes , Injeções Intraperitoneais , Injeções Intravenosas , Radioisótopos do Iodo , Marcação por Isótopo , Microscopia Eletrônica de Varredura , Compostos de Organossilício/química , Compostos de Organossilício/toxicidade , Poliaminas/toxicidade , Polietilenoglicóis/química , Polietilenoglicóis/toxicidade , Ratos , Ratos Wistar , Succinimidas , Distribuição TecidualRESUMO
Dichlorosilicon phthalocyanine (Cl2SiPc) and bis(tri-n-hexylsiloxy) silicon phthalocyanine (HexSiPc) have been evaluated in vitro as potential photosensitizers for photodynamic therapy (PDT) against the human amelanotic melanoma cell line M6. Each photosensitizer is dissolved in a solvent-PBS mixture, or entrapped in egg-yolk lecithin liposomes or in Cremophor EL micelles. The cells are incubated for 1 h with the sensitizer and then irradiated for 20 min, 1 h or 2 h (lambda > 480 nm, 10 mW cm-2). The photocytotoxic effect is dependent on the photosensitizer concentration and the light dose. Higher phototoxicity is observed after an irradiation of 2 h: treatment with a solution of photosensitizer (2 x 10(-9) M) leads to 10% (HexSiPc in egg-yolk lecithin liposomes) or 20% (Cl2SiPc in DMF-PBS solution) cell viability. After 1 h incubation and 20 min of light exposure, the photodynamic effect is connected with the type of delivery system used. For HexSiPc, lower cell viability is found when this photosensitizer is entrapped in egg-yolk lecithin instead of solvent-PBS or for Cremophor EL micelles with Cl2SiPc. Liposome-delivered HexSiPc leads to lipid damage in M6 cells, illustrated by an increase of thiobarbituric acid-reacting substances (TBARs), but the change is not significant with Cremophor EL. The same is observed for the antioxidative defences after photodynamic stress. The cells irradiated with HexSiPc entrapped in liposomes display an increase of superoxide dismutase (SOD) activity and a decrease of glutathione (GSH) level, glutathione peroxidase (GSHPx) and catalase (Cat) activities.
Assuntos
Indóis/metabolismo , Compostos de Organossilício/metabolismo , Fármacos Fotossensibilizantes/metabolismo , Silanos , Catalase/metabolismo , Linhagem Celular , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Humanos , Indóis/toxicidade , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Metabolismo dos Lipídeos , Melanócitos/efeitos dos fármacos , Melanócitos/metabolismo , Estrutura Molecular , Compostos de Organossilício/toxicidade , Fármacos Fotossensibilizantes/toxicidade , Superóxido Dismutase/metabolismo , Células Tumorais CultivadasRESUMO
A decrease in the efficacy of photodynamic therapy (PDT) with phthalocyanine photosensitizers was observed for lymphoblastic murine and human cell lines as the time between the addition of the photosensitizer, aluminum phthalocyanine (AIPc), to the culture medium and exposure to light was increased from 4 h to 18 h. The total intracellular concentration of photosensitizer did not decrease significantly during this 18 h interval. For the murine cell lines, the maximum cytotoxic and mutagenic effects were observed when the time between addition of the photosensitizer and irradiation was between 1 and 4 h. The time course of the variations in efficacy did not vary greatly from one murine cell line to another, even though the cell lines differ markedly in the extent of their cytotoxic and mutagenic response. The time course of the variation was similar for cytotoxicity and mutagenicity, as well as for the induction of DNA fragmentation. The human lymphoblastic cell line, WTK1, showed less variation in survival and mutability with time than did the murine cell lines. With Pc 4 (HOSiPcOSi[CH3]2[CH2]3N[CH3]2) as the photosensitizer, the photocytotoxicity for murine L5178Y (LY)-S1 cells did not change significantly as the time between addition of Pc 4 and irradiation was increased from 2 to 18 h. However, the mutagenicity decreased by a factor of three during this interval. The mutagenicity of PDT with Pc 4 was much less in LY-S1 cells than that with AlPc. The results suggest that the variation in the efficacy observed for AlPc-induced photocytotoxicity is caused by changes in the intracellular distribution and/or the aggregation of the photosensitizer with time after its addition.
Assuntos
Indóis/farmacocinética , Indóis/toxicidade , Compostos Organometálicos/farmacocinética , Compostos Organometálicos/toxicidade , Compostos de Organossilício/farmacocinética , Compostos de Organossilício/toxicidade , Fármacos Fotossensibilizantes/farmacocinética , Fármacos Fotossensibilizantes/toxicidade , Silanos , Alumínio/farmacocinética , Alumínio/toxicidade , Animais , Linfócitos B , Transporte Biológico , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Humanos , Leucemia L5178 , Luz , Camundongos , Camundongos Endogâmicos DBA , Células Tumorais Cultivadas , Raios XRESUMO
Platinum (II) and platinum (IV) coordination complexes derived from beta-silyl-substituted amines were prepared. The solubility of selected complexes in water and physiological saline was measured, and the effect of the beta-silicon on the reactivity of the complex in aqueous solution was determined by HPLC. The stabilities of selected silyl complexes were compared to the carbon analogues. The cyclic complexes 2a ("silaplatin") and its Pt(IV) analogue, 2b, were very active against L1210 leukemia in vivo. Both the platinum (II) complex 2a and the platinum (IV) complex 2b produced a significant number of cures over the dose range 10-40 mg/kg. The platinum (II) complex 2a, silaplatin, was very active in vivo against an L1210 leukemia subline that was resistant to cisplatin; 2a was also active, when given ip, against ic implanted L1210. The cyclobutanedicarboxylic acid complex 3c was synthesized; this complex was active against both cisplatin sensitive and resistant L1210 leukemia but was less potent than the analogous dichloro compound 2a. The acyclic platinum (II) and platinum (IV) complexes 1a,b were synthesized and unexpectedly found to be inactive in vivo against L1210 leukemia. More lipophilic silaplatin analogues were prepared--Pt(II) complex 2c and Pt(IV) complex 2d have one additional methylene carbon compared to 2a,b, whereas Pt(II) complex 2e and Pt(IV) complex 2f have two additional methylene carbons. Cyclization of the alkyl groups attached to the silicon gave the spiro bicyclic Pt(II) complexes 10a and 11a and the Pt(IV) complexes 10b and 11b.