Conocimientos, actitudes y prácticas sobre prevención y control de infecciones (PCI) en alumnos de Estomatología de la UAM-Xochimilco / Knowledge, attitudes and practices on infection prevention and control in Stomatology students UAM-Xochimilco

Introducción: la prevención y control de infecciones (PCI) pretenden prevenir, detener y propagar enfermedades infecciosas en pacientes y trabajadores de la salud. Este enfoque debe comenzar con la formación y capacitación del profesional de la salud, inmunizaciones recomendadas y ejecución de medidas universales de protección. Objetivos: evaluar conocimientos, actitudes y prácticas en la prevención y control de infec- ciones de los estudiantes y pasantes de servicio social de la licenciatura en Estomatología de la UAM-X. Material y métodos: se realizó un estudio descriptivo, transversal y observacional mediante una encuesta anónima como instrumento para recolección de datos a una muestra de 108 alumnos y pasantes. Resultados: se encontró que al contestar el interrogatorio sobre «prevención y control de infecciones¼, 59.3% tuvo un nivel de conocimientos aceptable y 40.7% tuvo un nivel muy bueno. De los encuestados, 87% desinfecta y cambia las barreras de protección entre cada paciente, 9.3% al comenzar y finalizar el día; el restante 3.7%, cuando está visiblemente sucio o cuando hay derrame de sustancias de líquidos corporales. Conclusión: los encuestados tienen adecuados conocimientos y prácticas, lo que infiere que tienen el sustento teórico y habilidades para enfrentarse a la etapa postpandemia para la atención de pacientes (AU)

ntroduction: infection prevention and control (IPC) efforts to prevent, stopping and spreading infectious diseases in patients and healthcare workers. This approach should begin with education and training of the health professional, recommended immunizations and implementation of universal protective measures. Objectives: to evaluate knowledge, attitudes and practices in infection prevention and control stomatology career's students and social service interns UAM-X. Material and methods: a descriptive, cross-sectional and observational study was carried out using an anonymous survey as an instrument for data collection from a sample of 108 students and interns. Results: it was found that when answering the questionnaire on «infection prevention and control¼, 59.3% had an acceptable level of knowledge and 40.7% had a very good level. 87% of the respondents disinfect and change the protective barriers between each patient, 9.3% at the beginning and end of the day; the remaining 3.7% when visibly dirty or when there is spillage of body fluids. Conclusion: respondents have adequate knowledge and practices, inferring that they have the theoretical support and skills to face the post-pandemic stage of patient care (AU)

La COVID-19 y sus repercusiones en la práctica odontológica / COVID-19 and their repercussions on dental practice

La llegada del COVID-19 generó diversos cambios significativos en el área de la salud y particularmente en el área odontológica, donde se priorizaron los tratamientos de urgencia relegando los procedimientos menores y atención de citas a la vía virtual. Otras modificaciones se concentraron en el uso de equipos de protección personal y logística de atención tanto para el paciente como para el profesional, siguiendo las indicaciones de las normas diseñadas para esta función; adicionalmente se hicieron adecuaciones en las áreas de recepción y práctica odontológica, y se capacitó a los profesionales en temas relativos a la enfermedad. El objetivo del presente artículo es analizar las implicaciones generadas en la práctica odontológica durante la pandemia por COVID-19 en los ámbitos de triaje, infraestructura, normativa y capacitación (AU)

The arrival of COVID-19 produced a series of significant changes in the health area and particularly in the dental area where emergency treatments were prioritized, relegating minor procedures and appointment care to the virtual route, other modifications focused on the use of personal protective equipment and care logistics for both the patient and the professional following the indications of the standards designed for this function, additionally adjustments were made in the reception and dental practice areas, and professionals were trained on issues related to the disease. The objective of this article is to analyze the implications generated in dental practice during the COVID-19 pandemic in the areas of triage, infrastructure, regulations and training (AU)

Bioseguridad en el laboratorio dental / Biosafety in the dental laboratory

Una enfermedad infecciosa es aquella producida por un agente infeccioso (bacterias, hongos, virus, etc.) que ingresa y se desarrolla en el organismo de un hospedero. Posteriormente, puede trasmitirse de un individuo a otro directamente por contacto entre ambos, o bien, indirectamente, por medio de un vec-tor biológico (de naturaleza animal o vegetal), o de un fómite (objeto inanimado). Las vías por las que un agente infeccioso puede ingresar a un hospedero son: inhalación (respiración de aerosoles), ingestión (salpicaduras de gotas), penetración de mucosas (na-sal, ocular y bucal) o lesiones en la piel o mucosas. Las fuentes de infección pueden ser los pacientes, el personal del consultorio o laboratorio, las superficies e instrumental contaminados y las prótesis o com-ponentes de éstas. Para evitar la propagación de los agentes microbianos se debe interrumpir el proceso de transmisión de los mismos. Todo profesional debe fortalecer y readecuar normas y protocolos de biose-guridad en la tarea diaria, para minimizar el riesgo de transmisión directa y cruzada entre el profesional, su equipo auxiliar, el laboratorista y los pacientes (AU)

An infectious disease is one caused by an infectious agent (bacteria, fungi, virus, etc.) that enters and develops in a host. Then it can be transmitted from one individual to another directly by contact between the two or, indirectly through a biological vector (an animal or plant nature), or a fomite (an inanimate object). The routes by which an infectious agent can enter a host are: inhalation (breathing of aerosols), ingestion (splash of droplets), penetration of mucous membranes (nasal, ocular and oral) and skin or mucous lesions. Sources of infection can be patients, office or laboratory personnel, contaminated surfaces and instruments and the prosthesis or component thereof. To prevent the spread of microbial agents, the process of their transmission must be interrupted. Every professional must strengthen and readjust biosafety standards and protocols in daily work to minimize the risk of direct and cross-transmission between the professional, his auxiliary team, the laboratory technician and the patients (AU)

Consideraciones para la atención de tratamientos endodóncicos en el contexto de la pandemia de COVID-19 / Considerations for the care of endodontic treatments in the context of the COVID-19 pandemic

La enfermedad COVID-19 fue declarada pandemia por la Organización Mundial de la Salud el 11 de marzo de 2020. El síndrome respiratorio agudo severo coronavirus-2 (SARS-CoV-2) es un virus transmitido de animales a seres humanos, altamente contagioso, cuyo periodo de incubación es de uno a 14 días. Los momentos por los que atraviesan la mayoría de los países debido a la pandemia declarada del COVID-19 han llevado a la proclamación de órdenes gubernamentales y/o recomendaciones en relación a la atención dental que debe dispensarse. En el momento actual, no se dispone de protocolos específicos oficiales, ni nacionales ni internacionales, que aborden de manera clara cómo debe proceder el odontólogo en su práctica diaria en la etapa posterior al confinamiento para trabajar con las mejores garantías de protección para los pacientes y el equipo humano de la consulta odontológica. Este estudio tuvo como objetivo analizar consideraciones para la atención de tratamientos endodóncicos en el contexto de la pandemia de COVID-19. Para lograr este objetivo se realizó una revisión bibliográfica en diferentes bases de datos, como: Google Scholar, Springer Link, Scopus, PubMed, para ello se emplearon estrategias de búsqueda, utilizando descriptores como: «endodontic¼, «recomendations¼, «odontology¼, «COVID-19¼, «pandemic¼ y operadores booleanos, con la finalidad de obtener información relevante y precisa. Contribuyendo a la práctica de la endodoncia con un protocolo general para el manejo de emergencias que muestre el fundamento del diagnóstico, los procedimientos clínicos y el uso de equipos de protección personal y barreras en el consultorio odontológico durante el brote de COVID-19 (AU)

The COVID-19 disease was declared a pandemic by the World Health Organization on March 11, 2020. The severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a virus transmitted from animals to humans, highly contagious, whose incubation period is one to 14 days. The times that most countries are going through due to the declared COVID-19 pandemic have led to the proclamation of government orders and/or recommendations regarding the dental care that should be provided. At the present time, there are no specific official national or international protocols that clearly address how the dentist should proceed, in their daily practice, in the post-confinement stage, to work with the best guarantees of protection for the patients and the human team of the dental practice. The objective of this study was to analyze considerations for the care of endodontic treatments in the context of the COVID-19 pandemic. To achieve this objective, a bibliographic review was carried out in different databases, such as: Google Scholar, Springer Link, Scopus, PubMed, for this, search strategies were used, using descriptors such as: «endodontic¼, «recommendations¼, «odontology¼, «COVID-19¼, «pandemic¼ and Boolean operators, in order to obtain relevant and accurate information. Contributing to the practice of endodontics a general protocol for emergency management that shows the rationale for diagnosis, clinical procedures and the use of personal protective equipment and barriers in the dental office during the COVID-19 outbreak (AU)

Presencia de bacterias en prótesis dentales durante el proceso de elaboración / Presence of bacteria in dental prostheses during the elaboration process

Existe una creciente preocupación sobre el tema de la infección cruzada en clínicas y laboratorios dentales. El laboratorio odontológico debe seguir normas de bioseguridad que garanticen a todo el equipo de salud la prevención de estas infecciones. Los técnicos que allí laboran corren el riesgo de exponer su cara a salpicaduras, así como a rocíos de sangre y saliva. Este estudio fue diseñado para saber si los laboratorios a los que recurrimos cumplen con estas normas de bioseguridad, y qué tan confiados podemos estar de la desinfección por parte de ellos, ya que las prótesis deberían estar desinfectadas correctamente antes de colocarlas en boca (AU)

There is growing concern about the issue of cross infection in dental clinics and laboratories. The dental laboratory must follow biosafety standards that guarantee the prevention of these infections to the entire health team. The technicians who work there run the risk of exposing their face to splashes and spray of blood and saliva. This study was designed to find out if the laboratories we use comply with these biosafety standards, and how confident we can be of their disinfection by them, since the prostheses should be properly disinfected before placing them in the mouth (AU)

Profesionales de odontología y la COVID-19: resultado de una encuesta / Dental professionels and the COVID-19: results from a survey

A partir de una propuesta del Comité de Investigaciones del Ateneo Argentino de Odontología se realizó una encuesta anónima transversal para conocer el estado de los odontólogos en relación con la infección provocada por la covid-19 ya que los afecta de una manera especial debido a las características del virus SARS-CoV-2 y su forma de trasmisión (AU)

From a proposal of the Research Committee of the Argentine Athenaeum of Dentistry, an anonymous cross-sectional survey was conducted to know the status of dentists in relation to the infection caused by covid-19 and that particularly affects dentists due to the characteristics of the SARS-CoV-2 virus and its form of transmission (AU)

Procedimientos libres de aerosol ante COVID-19 en odontología pediátrica / Aerosol-free procedures against COVID-19 in pediatric dentistry

A finales de 2019 se identificó el virus SARS-CoV-2 (por su significado en inglés Severe Acute Respiratory Syndrome Coronavirus 2) como agente etiológico de la COVID-19 (por su significado en inglés coronavirus disease 2019) en la ciudad de Wuhan, China. Debido a su rápida propagación al resto del mundo durante el primer trimestre del año 2020, la Organización Mundial de la Salud (OMS) la declaró pandemia mundial en marzo del mismo año. Por el potencial de contagio de COVID-19 se ha considerado que el entorno clínico en el que se desenvuelve la odontología puede ser de alto riesgo para el paciente, el odontólogo y sus asistentes si no se tienen las medidas de bioseguridad adecuadas. En un principio se vieron suspendidas las consultas regulares; sin embargo, al volver a la actividad laboral se han adaptado protocolos para el control de infecciones como reforzar el uso de barreras de protección y minimizar tratamientos que involucren aerosoles. La caries es uno de los principales motivos de consulta en la odontología pediátrica, por lo que en este escrito se sugieren algunos protocolos basados en la mínima invasión que prescinden de instrumental rotatorio para salvaguardar al paciente en riesgo de contagio, reduciendo el número de visitas y tiempo en consulta e incluso controlando algunos aspectos de salud bucal fuera de consulta clínica por medio de estrategias preventivas que pueden llevarse a cabo desde casa. Esto significa también mantener la tranquilidad por parte de los tutores del paciente ante la pandemia que se vive actualmente (AU)

At the end of 2019, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was identified as the etiological agent of COVID-19 in the city of Wuhan China. Due to its rapid spread to the rest of the world during the first trimester of 2020, the WHO declared a global pandemic in March of the same year. Due to the contagion potential of COVID-19, it has been considered that the clinical environment in which dentistry operates may be in high risk for the patient if the appropriate biosafety measures are not taken, initially clinical practices were suspended. However, when returning to work, protocols have been adapted to the infection control procedures, reinforced the use of protective barriers, and minimize treatments that involve aerosols. Caries is one of the main reasons for consultation in Pediatric Dentistry, this article suggests some protocols based on minimal invasion that dispense with rotating instruments to safeguard the patient from the risk of contagion, reducing the number of visits and time in consultation and even controlling some aspects of the oral health outside the dental visit through preventive strategies that can be carried out from home. Modifications to Dental Home. This should include maintaining tranquility and calm on the part of the patient's tutors in the face of the pandemic that we are currently experiencing (AU)

Covid-19: una mirada hacia la seguridad del paciente en odontología / Covid-19: a look towards patient safety in dentistry

La pandemia de enfermedad por coronavirus de 2019 (Covid-19) se ha convertido en un importante desafío de salud pública para todos los países. Las medidas de control de infecciones son necesarias para disminuir la circulación del virus y evitar que se siga propagando mundialmente. Debido a las características de los entornos odontológicos, el riesgo de infección cruzada entre los pacientes y los odontólogos es alto. Es por esto que se requieren protocolos de control de infecciones estrictos y efectivos. Elaborar estrategias de gestión del riesgo en salud en odontología permitirá brindar un entorno de seguridad para el paciente, el profesional y su equipo (AU)

The Covid-19 pandemic has become a major public health challenge all over the world. Infection control measures are necessary to prevent the virus from spreading further and to help to control the situation worldwide. Due to the characteristics of the dental environment, the risk of cross infection is high between patients and dentists. It is important to develop strict infection control protocols to provide a safety environment for the patient and the dental staff (AU)

Propuesta del modelo para control de infecciones en la consulta odontológica ante la pandemia de COVID-19 / Proposal for an infection control protocol in the dental consultation against the COVID-19 pandemic

Catalogada su labor como Servicios de Salud Indispensables, el profesional de la odontología se ha mantenido al pendiente de sus pacientes durante la pandemia de COVID-19, brindando atención de urgencia a quien lo solicita. A su regreso a la práctica típica, el odontólogo encontrará un entorno distinto o NUEVA NORMALIDAD, viéndose obligado a hacer modificaciones clínicas pertinentes y preparar a su equipo de trabajo para continuar brindando atención de calidad con un margen de seguridad amplificado, que de igual manera proteja a pacientes y al equipo de trabajo. Ante la falta de una vacuna que brinde protección y de tratamiento específico contra el SARS-CoV-2 (COVID-19), elevar el nivel de control de infecciones en el consultorio se vuelve ineludible y posiblemente irreversible en el quehacer odontológico cotidiano. En este artículo se propone un Protocolo de Control de Infecciones en la consulta odontológica que permita brindar seguridad en la atención bucal, considerando COVID-19, útil también para control infeccioso de otros patógenos virales o bacterianos (AU)

Considered dentistry as an Indispensable Health Services, during the COVID-19 pandemic the dental professional has been providing mostly emergency care. Upon return to typical dental practice, the dentist will find a different environment or NEW NORMALLY, being forced to make relevant clinical modifications and prepare his team to continue providing quality care with an amplified safety margin, which equally protects patients and dental team. In the absence of an available vaccine that provides protection or a specific treatment against SARSCoV- 2 (COVID-19), raising the level of infection control at the dental office will become unavoidable and possibly irreversible in the future quotidian dental work. This article proposes an Infection Control Protocol for the dental consultation that allows provide safety oral care, considering COVID-19, also useful for other viral or bacterial pathogens control (AU)

Manejo del paciente en atención odontológica y bioseguridad del personal durante el brote de coronavirus SARS-CoV-2 (COVID-19) / Patient management in dental care and staff biosecurity during the SARS-CoV-2 coronavirus outbreak (COVID-19)

El surgimiento de infecciones originadas por el SARS-CoV-2 en el humano ha desencadenado una serie de conflictos de salud, económicos y sociales en el entorno mundial. El área odontológica debe poseer todo el conocimiento necesario acerca de esta pandemia debido a que, como profesionales de la salud y responsables de la condición bucal de la sociedad, es de vital importancia disminuir el riesgo que presentan las enfermedades crónicas no transmisibles, cáncer, obesidad y otras, así como de los adultos mayores para que no generen un problema de salud severo y que además puedan ser asociadas con la presencia del COVID-19. Es importante conocer cuáles son las pautas por considerar en la práctica odontológica para una atención odontológica oportuna y eficaz manteniendo la bioseguridad del personal de salud. Por lo tanto, se genera esta guía de atención odontológica basada en la evidencia científica publicada para el conocimiento y dominio del profesional de salud oral (AU)

The emergence of human infections caused by SRAS-CoV-2 has triggered a series of health, economic and social conflicts in the global environment. The dental area must have all the necessary knowledge about this pandemic because, as a health professional and responsible for the oral condition of society, it is vitally important to reduce the risk of chronic non-communicable diseases, cancer, obesity and others, as well as older adults so that they do not generate a health problem and that may also be associated with the presence of COVID-19. It is important to know the problems of the guidelines to consider in dental practice for effective dental care and biosafety of health personnel. Therefore, this dental care guide is generated based on published scientific evidence for the knowledge and domain of the oral health professional (AU)

Análisis bacteriológico de piezas de mano de alta velocidad utilizadas en la práctica clínica / Bacteriological analysis of high speed handpieces used in clinical practice

Los profesionales de la salud están expuestos a una gran variedad de microorganismos desde esporas, bacterias, hongos, virus y protozoarios que pueden encontrarse en la sangre y/o saliva de los pacientes. Cualquiera de estos microorganismos puede causar una enfermedad infectocontagiosa a través de pinchazos y/o salpicaduras producidas por el aerosol durante la práctica dental. Objetivo: Determinar la presencia bacteriana en las piezas de alta velocidad utilizadas en la práctica clínica. Material y métodos: Es un estudio experimental, observacional y transversal en el que se evaluó la contaminación de 30 piezas de alta velocidad utilizadas en la práctica clínica. Previo al estudio se efectuó una estandarización obteniendo una kappa del 0.85. Se realizó una base de datos en el programa SPSS versión 22, con el que se llevó a cabo el análisis descriptivo para determinar medidas de tendencia central. Resultados: 73.3% de las muestras analizadas tuvieron crecimiento bacteriano, entre las bacterias que se encontraron resultó que 54.5% de ellas fueron bacterias Gram positivas y el resto Gram negativas. La bacteria con mayor presencia en la muestra fue el Bacillus en 45.5% seguida del Streptococcus en 27.3%, el restante 27.2% fue Staphylococcus, Coccus y Streptobacillus. Conclusiones: El uso correcto de las piezas de alta, así como su desinfección en la consulta dental es de suma importancia, ya que nos ayudan a evitar contaminaciones cruzadas y a prevenir que dentro del área de trabajo se formen focos de infección (AU)

Health professionals are exposed to a wide variety of microorganisms from spores, bacteria, fungi, viruses and protozoa that can be found in the blood and/or saliva of patients. Any of these microorganisms can cause an infectious disease through punctures and / or splashes produced by the aerosol during dental practice.1,2 Objective: To determine the bacterial presence in the high-speed pieces used in clinical practice. Material and methods: It are an experimental, observational and transversal study; where the contamination of 30 high-speed pieces used in clinical practice was evaluated. Prior to the study, a standardization was made obtaining a kappa of 0.85. A database was made in the program SPSS version 22, with which the descriptive analysis was carried out to determine measures of central tendency. Results: 73.3% of the analyzed samples showed bacterial growth, among the bacteria that were found, 54.5% of them were gram-positive bacteria and the rest were gram-negative. The bacterium with the highest presence in the sample was for Bacillus in 45.5% followed by Streptococcus in 27.3%, the remaining 27.2% was for Staphylococcus, Coccus and Streptobacillus. Conclusions: The correct use of the discharging parts, as well as their disinfection in the dental practice is of the utmost importance as they help us to avoid cross contamination and to prevent foci of infection from forming within the work area (AU)

Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines / Evaluación Microbiológica del Agua de Biofilm Adherido a Líneas de Agua de la Unidad Dental

ABSTRACT: The contamination of the dental units' waterlines is a reality, which can develop individual and collective disorders. The aim of this study was to evaluate the prevalence and resistance profile of bacteria on the internal surfaces of waterlines in a dental clinic from a Dentistry school of a Brazilian university. The design was an exploratory, descriptive study with quantitative and qualitative approach. Samples (n=4) were collected for analysis at different points: the portion closest to the water reservoir of the chair, and the portion closest to the triple syringe. After collection the samples were cultured in BHI medium in an oven for 24-48 h at 37 °C. For the quantitative analysis 1 ml of each sample was used for serial dilution up to the dilution value seven. The colonies were counted after pour plate and the results expressed in UFC/cm2. The qualitative analysis was initiated with the cultivation of Agar Blood, EMB-Levine and Cetrimide Agar for 24 h, and the identification of bacteria was performed by microscopic analyses. The resistance profile was verified by classical antibiogram. The internal surfaces of unit waterlines units exhibited a mean of 2.44 x 109 CFU / cm2. Bacillus subtilis and Pseudomonas aeruginosa were identified. The resistance profile of Pseudomonas aeruginosa indicated sensitivity to all tested antibiotics. A large number of microorganisms was quantified from biofilm accumulated in the dental units' waterlines. However, they were not resistant to classic antibiogram. Better management and application of decontamination protocols for waterlines need to be applied since opportunistic infections may be associated with Pseudomonas aeruginosa.

RESUMEN: La contaminación de líneas de agua en las unidades dentarias es una realidad, generando enfermedades individuales y colectivas. El objetivo de este estudio fue evaluar la prevalencia y resistencia de las bacterias en las zonas internas de las líneas de agua de la Facultad de Odontología de una universidad brasileña. El diseño del estudio fue exploratorio, descriptivo con enfoques cuantitativos y cualitativos. Las muestras para análisis (n=4) fueron selecionadas de distintos lugares: el punto más cercano al sistema de agua del sillón odontológico y el punto más cercano a la jeringa tríplice. Las muestras obtenidas fueron cultivadas en un médio BHI por 24-48 h, en un horno a 37 ºC. Para el análisis cuantitativo, se utilizó 1 ml de cada muestra para dilución hasta el valor siete. Las colonias fueran contadas y los resultados fueron expresados en UFC/cm2. El análisis cualitativo fue iniciado con el cultivo de Agar Sangre, EBM-Levine y Agar Cetrimide por 24 h y la identificación de la bacteria fue realizada por análisis microscópicos. El perfil de resistencia fue verificado por el antibiograma clásico. Las zonas internas de las unidades de líneas de agua mostraron un promédio de 2,44 x 109 UFC/cm2. Bacillus subtilis y Pseudomonas aeruginosa fueron encontrados. El perfil de resistencia de Pseudomonas aeruginosa indicó sensibilidad a todos los antibióticos testados. Un gran número de microorganismos fue cuantificado desde la biopelícula acumulada en las líneas de agua de unidades dentales. Sin embargo, no resistieron al antibiograma clásico. Se requiere una mejor gestión y aplicación de protocolos de decontaminación en las líneas de agua debido a que las infecciones oportunistas puedan estar asociadas a Pseudomonas aeruginosa.

Percepção das precauções padrão, prática do reencape de agulhas e condutas frente a acidente com material biológico de equipes de saúde bucal do serviço público odontológico / Perception of the standard precautions, practice of re-capping of needles and practices when facing an accident involving biological material of dental health teams at dental public service

Resumo: O presente estudo objetivou verificar o conhecimento e adesão às precauções padrão (PP), em especial a prática de não reencapar agu lhas; as ações a serem tomadas diante acidentes e aspectos relativos à prevenção de infecções, pelas equipes de saúde bucal da rede públi ca de um município do Estado de São Paulo, Brasil. Também observar os materiais descartados nos recipientes do grupo E em todas as unidades de atendimento odontológico do município. Aplicou-se um questionário semiestruturado, com perguntas direcionadas ao tema. Dos 79 pesquisados, 60 responderam ao questionário, onde 33 (55%) eram cirurgiões-dentistas. 34 (56,6%) profissionais não sabiam o que eram as PP e 43 (71,6%) afirmaram reencapar agulhas. 27 (44,9%) já sofreram algum tipo de acidente com material perfurocortante, contaminado ou sangue e 44 (73,3%) desconheciam ou se equivocaram sobre quais os cuidados imediatos em caso de exposição cutânea ou percutânea. Em relação à análise dos recipientes de descarte, observaram-se 5.193 agulhas descartadas, sendo 3.790 (73%) reencapadas em um, ou ambos os lados. Também o descarte incorreto de materiais não perfurocortantes. Conclui-se que existe falha no conhecimento sobre precauções padrão e condutas em casos de acidentes com mate rial biológico, além do incorreto descarte de materiais nos recipientes do grupo E.

Abstract: This study aimed to verify the knowledge and adoption of the stan dard precautions (PP), especially in the practice of not re-capping needles; the actions to be taken in relation to accidents and aspects related to the prevention of infections, by the dental health teams of the public service of a city in the State of São Paulo, Brazil. We also assessed the materials discarded in the containers of group E in all dental care units of the city. A semi-structured questionnaire was applied, with questions about the theme. Of the 79 subjects surveyed, 60 answered the questionnaire, where 33 (55%) were dental surgeons. 34 (56.6%) professionals did not know what PP were and 43 (71.6%) reported re-capping needles. 27 (44.9%) had already suffered some kind of accident with sharp tools or contaminated material with blood and 44 (73.3%) were unaware or made mistakes about the immediate care in case of skin or percutaneous exposure. Regarding the analysis of the disposal containers, 5,193 discarded needles were observed, of which 3,790 (73%) were re-capped on one or both sides. Also, we observed the improper disposal of non- sharps material. We concluded that there is a lack of knowledge about standard precautions and conducts in cases of accidents with biological material, besides the incorrect disposal of materials in the containers of group E.

Antibacterial Effect of Surface Pretreatment Techniques against Streptococcus Mutans.

OBJECTIVE: The aim of this study was to evaluate the antibacterial surface pretreatment methods against Streptococcus mutans within the infected dentin surface using a tooth cavity model. MATERIAL AND METHODS: Seventy-two cavities were prepared on caries-free third molars (n = 8). After sterilization, teeth were inoculated with S. mutans for 48 h. One cavity of each tooth was used to evaluate the infection. Following inoculation, infected cavity surfaces were treated either with (1) Er:YAG Laser (1W; 5x5s, Smart 2940D Plus, Deka Laser), (2) Ozone (80s; HealOzone, Kavo), (3) ErYAG-Ozone combination, (4) Er:YAG-Ozone-CHX combination, (5) Chlorhexidine (CHX), (6) Clearfil Protect Bond (PB), (7) potassium-titanyl-phosphate (KTP) Laser (1W; 60 s, SMARTLITE D, Deka Laser), (8) KTP-Ozone combination, and (9) KTP-Ozone-CHX. Standardized amounts of dentin chips were obtained from the cavity walls, and the number of bacteria recovered was counted. Kruskal-Wallis test was used for statistical analyzes. RESULTS: Both sole antibacterial materials, CHX or Protect Bond application, exhibited the most effective antibacterial activity with 125 and 156 CFU is an acronym of "colony forming unit" usullay mentioned by acronym. (CFU/ml), respectively, among the groups evaluated (P < 0.05). Er:YAG laser irradiation and its combinations with other antibacterial surface pretreatment applications also inhibited the bacterial growth with, respectively, 1444, 406, and 294 CFU/ml bacterial recovery being more efficient than KTP laser irradiation and ozone combinations. CONCLUSIONS: As an alternative device with photodynamic effects, Er:YAG and KTP laser irradiations and their further combinations during the cavity pretreatment procedure with chlorhexidine and ozone treatments exerted antibacterial effect against S. mutans, whereas chlorhexidine and antibacterial dentin bonding application solely have the highest antibacterial effects.

Cleaning of used rotary nickel-titanium files in an ultrasonic bath by locally intensified acoustic cavitation.

AIM: To compare the pre-sterilization cleaning of rotary Ni-Ti files of different sizes previously used a. ex vivo and b. clinically by a washer-disinfector, a regular ultrasonic bath, and the same ultrasonic bath in combination with a recently developed cavitation intensifying method. METHODOLOGY: Two sets of two hundred rotary Ni-Ti files, one previously used ex vivo and another one used clinically, were collected from the undergraduate and postgraduate clinics of the Academic Centre for Dentistry Amsterdam (ACTA). The instruments were immersed in an enzymatic solution and subsequently cleaned either by a washer-disinfector, a regular ultrasonic bath combined with a glass beaker, the same bath combined with a beaker lined with two cavitation intensifying sheets or with two standard plastic sheets. The positive control consisted of used files that did not undergo any cleaning and the negative control included new unused files. The instruments were then stained to reveal remaining protein material and scored under a stereoscopic microscope. The results were analysed by nonparametric statistical tests (α = 0.05). RESULTS: No significant difference was found between the combination of the ultrasonic bath and the regular glass beaker and the same ultrasonic bath with the beaker lined with the cavitation intensifying sheets. The washer-disinfector left significantly more debris compared to the latter group when clinically used files were evaluated (P ≤ 0.001). The effect of instrument size on cleaning was not consistent. CONCLUSION: None of the tested methods was able to remove all residual protein material from the files; however, it could be noted that this study did not follow the reprocessing protocol provided by the manufacturer.

Uso de ultrassom e laser na descontaminação do sistema de canais radiculares: revisão de literatura / Use of ultrasound and laser in root canal decontamination: literature review

O maior obstáculo para o sucesso do tratamento endodôntico é a eliminação da microbiota instalada. A instrumentação mecânica, bem como as substâncias químicas auxiliares, é utilizada para eliminação dessa microbiota. Além das soluções irrigantes, outras técnicas complementares, como a agitação ultrassônica passiva (PUI) e o laser, estão sendo utilizadas para otimizar a eliminação dos microrganismos. Desse modo, o presente estudo objetivou o levantamento bibliográfico de diferentes tipos de agitação dos irrigantes no tratamento endodôntico. A PUI tem sido sugerida como um meio para melhorar o desbridamento do canal. Já o laser tem sido utilizado por apresentar vantagens terapêuticas ao tratamento. Todas as técnicas citadas para melhorar a limpeza dos canais radiculares mostraram alguma melhora. A técnica mais empregada ainda é a agitação ultrassônica, por apresentar eficiência e praticidade no procedimento. Pôde-se concluir que a diminuição da microbiota é mais eficiente quando se utiliza uma maior concentração da solução irrigadora, independentemente da agitação final de escolha.