Working with cattle slurry on farms: emission and dispersion of hydrogen sulfide gas during stirring.

Over the past 15 years, there have been numerous fatalities related to working with animal slurry. Working with cattle slurry releases toxic gases, in particular, hydrogen sulphide (H2S), which can cause acute central nervous system toxicity, breathing difficulties, and death if exposed to high concentrations. Real-time measurements of H2S gas were taken over distance and time, during the stirring of cattle slurry on farms. Gas was measured at eight slurry stores with differing typical configurations of indoor or outdoor stores and with or without slatted flooring. Highest H2S gas levels were measured from indoor stores under slatted floors, and generally at positions closest to the stirrer or the point of maximum stirring, with levels decreasing with distance from source. Most of the data indicate H2S gas levels increase very rapidly after stirring starts, and mostly decline to baseline levels within 30 min post start of stirring. There were, however, circumstances where gas levels remained high and only started to decline once the stirrer had stopped. H2S gas levels at all farms, at all positions measured were consistently below 10 ppm within 30 min of the stirrer being stopped. The current data highlight areas of the farm and ways of working that have the potential for workers and others to be at risk of exposure to toxic slurry gases. The area should be left to ventilate naturally for at least 30 min after the stirrer has been stopped before re-entering buildings. Influencing the design of stirring equipment and future slurry stores would likely reduce the risk of worker exposure to slurry gases.

Extended Sanitation Intervals for Cage Components and Automated Watering Valves: Validation and Cost Analysis.

Although the Guide suggests changing rodent cage components every 2 wk, it states that "decreased sanitation frequency may be justified if the microenvironment in the cages, under the condition of use ..., is not compromised." The purpose of this study was to evaluate extended sanitation intervals of cage components (automated watering valve, wire bar lid, and filter top) of mouse individually ventilated caging (IVCs) at our institution. We hypothesized that there would be no significant difference in relative light units measured by ATP luminometry of these cage components at the control time point of 14 d as compared with each extended time interval: 28, 56, and 84 d. In addition, for automated watering valves, the study was extended to 168 d. We also hypothesized that time-and-motion studies performed by moving to a sanitation interval of 84 d for all components would result in substantial time and cost savings. The components of a total of 24 cages containing 4 or 5 mice each were swabbed, and an ATP luminometer was used to detect organic matter. We found no significant differences in organic matter load between 14 d and all other time points for all cage components. Our time- and cost-savings analysis found that extending the sanitation interval of cage components from every 2 wk (14 d) to every 3 mo (84 d) for every 10,000 cages would save about 3,000 technician hours annually, for a total annual labor cost savings of about $100,000. This study is the first to validate the extended sanitation interval of automated watering valves and confirms the findings of previous studies that validated the extended sanitation frequency of wire bar lids and filter tops of rodent IVCs. Overall, extending the sanitation frequency of cage components reduces workload of animal care staff without compromising the cage microenvironment.

Putting zoo animal cancer into perspective.

As part of a comparative research agenda that promises insights that help extend the human lifespan and combat cancer, cancer prevalence in zoo animals has received recent attention. Here, we want to draw attention to a principle of cancer research that was introduced into the zoo world as early on as 1933, but that seems to have gone somewhat forgotten: Cancer is mainly a disease of old age, and therefore studies aiming at identifying taxa that are particularly susceptible or resistant to cancer must control for whether the respective zoo populations are 'old.' In a comparative context, 'old age' cannot be measured in absolute terms (e.g., years), but only in relation to a species' maximum lifespan: Species that achieve, across zoos, a higher mean lifespan as a percent of their maximum lifespan are 'older.' When applying this metric to former as well as more recently published data on cancer prevalence, it appears that those species that become relatively old in zoos-in particular, the carnivores-have a relatively high cancer prevalence. Any improvement in animal husbandry-which reduces premature deaths-should, by default, lead to more cancer. Cancer in zoo animals, like any other old-age condition, might therefore be embraced as a proxy for good husbandry. Rather than following a sensationalist approach that dramatizes disease and death per se, zoos should be clear about what their husbandry goals are, what relative longevities they want to achieve for which species, and what old-age diseases they should therefore expect: in the end, one has to die of something.

Effects and Mechanisms of Non-Thermal Plasma-Mediated ROS and Its Applications in Animal Husbandry and Biomedicine.

Non-thermal plasma (NTP) is an ionized gas composed of neutral and charged reactive species, electric fields, and ultraviolet radiation. NTP presents a relatively low discharge temperature because it is characterized by the fact that the temperature values of ions and neutral particles are much lower than that of electrons. Reactive species (atoms, radicals, ions, electrons) are produced in NTP and delivered to biological objects induce a set of biochemical processes in cells or tissues. NTP can mediate reactive oxygen species (ROS) levels in an intensity- and time-dependent manner. ROS homeostasis plays an important role in animal health. Relatively low or physiological levels of ROS mediated by NTP promote cell proliferation and differentiation, while high or excessive levels of ROS mediated by NTP cause oxidative stress damage and even cell death. NTP treatment under appropriate conditions not only produces moderate levels of exogenous ROS directly and stimulates intracellular ROS generation, but also can regulate intracellular ROS levels indirectly, which affect the redox state in different cells and tissues of animals. However, the treatment condition of NTP need to be optimized and the potential mechanism of NTP-mediated ROS in different biological targets is still unclear. Over the past ten decades, interest in the application of NTP technology in biology and medical sciences has been rapidly growing. There is significant optimism that NTP can be developed for a wide range of applications such as wound healing, oral treatment, cancer therapy, and biomedical materials because of its safety, non-toxicity, and high efficiency. Moreover, the combined application of NTP with other methods is currently a hot research topic because of more effective effects on sterilization and anti-cancer abilities. Interestingly, NTP technology has presented great application potential in the animal husbandry field in recent years. However, the wide applications of NTP are related to different and complicated mechanisms, and whether NTP-mediated ROS play a critical role in its application need to be clarified. Therefore, this review mainly summarizes the effects of ROS on animal health, the mechanisms of NTP-mediated ROS levels through antioxidant clearance and ROS generation, and the potential applications of NTP-mediated ROS in animal growth and breeding, animal health, animal-derived food safety, and biomedical fields including would healing, oral treatment, cancer therapy, and biomaterials. This will provide a theoretical basis for promoting the healthy development of animal husbandry and the prevention and treatment of diseases in both animals and human beings.

Nomenclature for standardized designation of diploid genotypes in genetically modified laboratory animals.

Information about the diploid genotype of a gene-modified or mutant laboratory animal is essential for breeding and experimental planning. It is also required for the exchange of animals between different research groups or for communication with professional genotyping service providers. While there are detailed, standardized rules for creating an allele name of a genome modification or mutation, the notation of the diploid genotype after biopsy and genotyping has not been standardized yet. Therefore, a uniform, generally understandable nomenclature for the diploid genotype of gene-modified laboratory animals is needed. With the here-proposed nomenclature recommendations from the Committee on Genetics and Breeding of Laboratory Animals of the German Society for Laboratory Animal Science (GV-SOLAS), we provide a practical, standardized representation of the genotype of gene-modified animals. It is intended to serve as a compact guide for animal care and scientific personnel in animal research facilities and to simplify data exchange between groups and with external service providers.

Cattle health in the Iron Age and Roman Netherlands.

OBJECTIVE: To investigate diachonic and regional trends in the occurrence of pathologies in cattle in the Iron Age and Roman Netherlands. A key objective is to investigate whether the intensification of cattle husbandry in the Roman period was associated with an increase in pathology. MATERIALS: The data set consists of 167 sites with a combined total of 127,373 individual specimens for cattle, sheep/goat, horse, and pig. METHODS: A quantitative approach was used, investigating the frequency of pathologies over time and per region. For cattle, pathology frequencies were also investigated per type. Several multi-period sites were considered in more detail. RESULTS: Pathology frequencies increased during the Iron Age and Roman period. In cattle, joint pathology was most common, followed by dental pathology. CONCLUSIONS: The overall frequency of pathology aligns with frequencies in other regions. Some pathological conditions in cattle can tentatively be linked to intensification, such as joint pathology at two sites in the Middle and Late Roman periods, an increase in dental pathology and trauma). SIGNIFICANCE: This review revealed diachronic trends and linked them to developments in animal husbandry and highlights the importance of recording and publishing pathological lesions. LIMITATIONS: The multi-causal origin of joint and dental pathology makes it difficult to relate them to the intensification of cattle husbandry. SUGGESTIONS FOR FURTHER RESEARCH: It is hoped that this review will stimulate further paleopathological research globally, especially systematic studies into foot pathologies.

Natural photosensitizer-loaded in micellar copolymer to prevent bovine mastitis: A new post-dipping protocol on milking.

Good management practices such as post-dipping applications (post-milking immersion bath) contribute to the dairy cattle health during lactation and minimize the appearance of mastitis (an infection in the mammary gland). The post-dipping procedure is performed conventionally using iodine-based solutions. The search for therapeutic modalities that are not invasive and do not cause resistance to the microorganisms that cause bovine mastitis instigates the interest of the scientific community. In this regard, antimicrobial Photodynamic Therapy (aPDT) is highlighted. The aPDT is based on combining a photosensitizer (PS) compound, light of adequate wavelength, and molecular oxygen (3O2), which triggers a series of photophysical processes and photochemical reactions that generate reactive oxygen species (ROS) responsible for the inactivation of microorganisms. The present investigation explored the photodynamic efficiency of two natural PS: Chlorophyll-rich spinach extract (CHL) and Curcumin (CUR), both incorporated into the Pluronic® F127 micellar copolymer. They were applied in post-dipping procedures in two different experiments. The photoactivity of formulations mediated through aPDT was conducted against Staphylococcus aureus, and obtained a minimum inhibitory concentration (MIC) of 6.8 mg mL-1 for CHL-F127 and 0.25 mg mL-1 for CUR-F127. Only CUR-F127 inhibited Escherichia coli growth with MIC 0.50 mg mL-1. Concerning the count of microorganisms during the days of the application, a significant difference was observed between the treatments and control (Iodine) when the teat surface of cows was evaluated. For CHL-F127 there was a difference for Coliform and Staphylococcus (p < 0.05). For CUR-F127 there was a difference for aerobic mesophilic and Staphylococcus (p < 0.05). Such application decreased bacterial load and maintained the milk quality, being evaluated via total microorganism count, physical-chemical composition, and somatic cell count (SCC).

A blend of functional amino acids and grape polyphenols improves the pig capacity to cope with an inflammatory challenge caused by poor hygiene of housing conditions.

BACKGROUND: Dietary supplementation with a blend of functional amino acids (AA) and grape extract polyphenols contributes to preserve intestinal health and growth performance of piglets during the post-weaning period. In the present experiment, we assessed if a supplementation with a mix of AA and grape extract polyphenols during the post-weaning period would persist to improve the pig capacity to cope with a subsequent challenge caused by poor hygiene of housing conditions. Eighty pigs weaned at 28 days of age were fed a standard diet supplemented (AAP) or not (CNT) with 0.2% of a blend of AA (glutamine, arginine, cystine, valine, isoleucine, and leucine) and grape extract polyphenols during the post-weaning period (from week 0 to 6). At week 6, pigs were transferred to a growing unit where 50% of pigs previously fed AAP and CNT diets were housed in good and the other 50% in poor hygiene conditions for 3 weeks (from week 7 to 9; challenge period). All pigs were fed a standard growing diet that was not supplemented with AAP. We measured pig growth performance, plasma indicators of inflammation, digestive integrity, and oxidative status, and scored fecal consistency. Differences were considered significant at P ≤ 0.05. RESULTS: One week post-weaning, pigs fed AAP had lower plasma concentrations of haptoglobin than CNT pigs (P = 0.03). Six weeks post-weaning, plasma concentrations of diamine oxidase (DAO) were lower (P = 0.03) whereas those of vitamin E and A were greater (P ≤ 0.05) in pigs fed AAP compared to CNT pigs. The prevalence of diarrhea was higher in CNT pigs compared to AAP pigs (P < 0.01). During the challenge period, only pigs previously fed CNT diet had lower growth rate in poor than good conditions (P ≤ 0.05). They had also greater plasma concentrations of haptoglobin and oxidative stress index (OSI) and lower plasma concentrations of vitamin E in poor than good hygiene conditions (P ≤ 0.05). CONCLUSIONS: Pigs fed AAP diet during post-weaning had less diarrhea and plasma concentrations of a digestive integrity marker, as well as greater plasma concentrations of antioxidant indicators during the post-weaning period. The beneficial effects of AAP supplementation persisted after the post-weaning period as evidenced by the absence of effects of the hygiene challenge on growth and health indicators in pigs previously fed APP. This clearly indicated a greater ability of pigs fed AAP to cope with the poor hygiene conditions.

A comprehensive review on natural phenolic compounds as alternatives to in-feed antibiotics.

Intensive livestock and poultry farming in China largely relied on the use of in-feed antibiotics until July 2020. The consequences of antibiotic overuse in animal feed include accumulation in animal products and the development of bacterial antibiotic resistance, both of which threaten food safety and human health. China has now completely banned the circulation of commercial feed containing growth-promoting drug additives (except Chinese herbal medicine). Therefore, alternatives to in-feed antibiotics in animal production are greatly needed. Natural phenolic compounds (NPCs) exist widely in plants and are non-toxic, non-polluting, highly reproducible, and leave little residue. Many natural flavonoids, phenolic acids, lignans, and stilbenes have polyphenol chemical structures and exhibit great potential as alternatives to antibiotics. In this review we delineate the characteristics of plant-derived NPCs and summarize their current applications as alternatives to in-feed antibiotics, aiming to provide new strategies for antibiotic-free feeding and promote the development of more sustainable animal husbandry practices.

Influence of institutional attributes on fulfillment of transfer and breeding recommendations in zoos and aquariums.

Compliance with Breeding and Transfer Plan (BTP) recommendations is important to promote long-term viability and meet the management goals of the Association of Zoos and Aquarium Species Survival Plans (SSPs®). However, individual institutions may fulfill recommendations at different rates, potentially compromising management efforts. We examined institution-level fulfillment rates of BTP recommendations (i.e, "Hold," "Send To," "Breed With," and "Do Not Breed"), and how rates differ based on institutional attributes related to resource levels, geographic location and climate, involvement in population management, and the SSPs in which institutions participated using conditional random forests and generalized linear mixed models. We analyzed 52,182 recommendations from 375 SSPs issued to 170 institutions in BTPs published from 2012 to 2019. Contrary to predictions, our results did not reveal large differences in fulfillment rates based on resources, geographic location, or climate variables at the institution level. We found modest patterns indicating that recommendation fulfillment for Hold and Do Not Breed recommendations in mammals was lower with longer interplanning periods, while fulfillment of Hold recommendations in birds increased with the average experience of individuals in SSP leadership roles. For herptiles, fulfillment of Send To recommendations was moderately higher in institutions with species spread across more herptile taxonomic advisory groups (the management unit for related species). Although our results suggest that overarching institutional factors do not have a strong influence on fulfillment rates at the scale of our study, more focused research within a specific temporal window, taxa, or suite of SSPs may reveal more informative patterns for population management.

Efecto de la deforestación ribereña sobre la materia orgánica particulada gruesa en quebradas con influencia ganadera de la región Andina de Colombia / Effect of riparian deforestation on coarse particulate organic matter in streams with livestock influence in the Andean region of Colombia

Introducción: La materia orgánica particulada gruesa originada en la vegetación ribereña es la fuente principal de energía en muchas cuencas de cabecera. Sin embargo, la transferencia de dicha materia es alterada por la destrucción de los bosques. Objetivo: Evaluar diferencias en la materia orgánica en quebradas con bosques y con pastizales. Métodos: Comparar las entradas, el almacenamiento y la exportación de esta materia orgánica en el cauce de quebradas con bosques y con pastizales, en la región central de los Andes de Colombia. En cada quebrada, se midieron los aportes verticales y laterales de hojarasca con canastas; hojarasca del lecho de las quebradas con cuadrantes, y la exportación de material con redes de deriva, con un alcance de 100 m. Resultados: Las quebradas con bosques ribereños recibieron un promedio anual de 915 g m-2 de materia orgánica particulada gruesa, exportando un total de 334 g m-3 y almacenando 732 g m-2, valores que fueron significativamente más altos que en quebradas con pastizales, donde los valores correspondientes fueron: 125.4 g m-2; 128 g m-3 y 205.5 g m-2. Conclusiones: La remoción de cobertura boscosa de la zona ribereña reduce la materia orgánica en estas cuencas de cabecera en Colombia.

Introduction: Coarse particulate organic matter originated in riparian vegetation is the main source of energy in many headwater streams. However, the transfer of such material is altered by the destruction of forests. Objective: To assess flow differences of this organic matter in streams with forests and grasslands. Methods: We compared input, storage and export of this organic matter in the riparian belts of streams with forests, and streams with grasslands, in the central Andean region of Colombia. For each stream, we measured vertical and lateral litter with baskets; stream bed litter with a quadrant, and matter export with drift nets, in a 100 m reach. Results: The streams with riparian forest received an average of 915 g m-2 of coarse particulate organic matter annually, exported a total of 334 g m-3 and stored 732 g m-2, values that were significantly higher than in grassland streams, where the corresponding values were: 125.4 g m-2; 128 g m-3 and 205.5 g m-2. Conclusions: The removal of tree cover from the riparian zone reduced the organic matter in these headwater streams of Colombia.

Polyphenols for Livestock Feed: Sustainable Perspectives for Animal Husbandry?

There is growing interest in specialized metabolites for fortification strategies in feed and/or as an antioxidant, anti-inflammatory and antimicrobial alternative for the containment of disorders/pathologies that can also badly impact human nutrition. In this context, the improvement of the diet of ruminant species with polyphenols and the influence of these compounds on animal performance, biohydrogenation processes, methanogenesis, and quality and quantity of milk have been extensively investigated through in vitro and in vivo studies. Often conflicting results emerge from a review of the literature of recent years. However, the data suggest pursuing a deepening of the role of phenols and polyphenols in ruminant feeding, paying greater attention to the chemistry of the single compound or to that of the mixture of compounds more commonly used for investigative purposes.

Effect of moving from being extensively managed out in pasture into training on the incidence of equine gastric ulcer syndrome in Icelandic horses.

OBJECTIVE: To investigate equine squamous gastric disease (ESGD) and equine glandular gastric disease (EGGD) in Icelandic horses moving from pasture into training. ANIMALS: 81 horses (median age, 3 years; interquartile range, 1 year) from 10 farms representing 4 different Icelandic regions. PROCEDURES: Initial gastroscopy was undertaken within 2 weeks of moving from pasture into a training establishment. A total of 71 horses underwent endoscopic examination again 8 weeks later. Various management and behavioral factors were assessed through face-to-face questionnaires with the owners or trainers. Multivariable logistic regression was used to determine factors contributing to any change in ESGD and EGGD severity score during the 8-week training period. RESULTS: Incidence of EGGD and ESGD in this feral population was similar to that found in domesticated horses. ESGD incidence (severity score, ≥ 2; score range, 0 to 4) reduced from an initial 71.6% (58/81) to 25.4% (18/71). On multivariable analysis, sex (ie, being a stallion or a female vs gelding) increased the likelihood of ulcer grade reduction. Being fed preserved forage 3 or more times a day also improved the likelihood of ESGD reduction (odds ratio, 17.95; 95% CI, 1.67 to 193.40; P = .017). Overall, the farm explained 35% of the variance, confirming the importance of management factors. Incidence of EGGD (severity score, ≥ 1; score range, 0 to 2) reduced from 47% (38/81) to 40.8% (29/71) during the same period. No measured variables were associated significantly with EGGD incidence or reduction. CLINICAL RELEVANCE: Pasture provision (without supplementary feed or forage) does not result automatically in a low incidence of gastric ulcers. Regular provision of preserved forage is a key factor in reducing ESGD incidence.

Refinements to rodent head fixation and fluid/food control for neuroscience.

The use of head fixation in mice is increasingly common in research, its use having initially been restricted to the field of sensory neuroscience. Head restraint has often been combined with fluid control, rather than food restriction, to motivate behaviour, but this too is now in use for both restrained and non-restrained animals. Despite this, there is little guidance on how best to employ these techniques to optimise both scientific outcomes and animal welfare. This article summarises current practices and provides recommendations to improve animal wellbeing and data quality, based on a survey of the community, literature reviews, and the expert opinion and practical experience of an international working group convened by the UK's National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs). Topics covered include head fixation surgery and post-operative care, habituation to restraint, and the use of fluid/food control to motivate performance. We also discuss some recent developments that may offer alternative ways to collect data from large numbers of behavioural trials without the need for restraint. The aim is to provide support for researchers at all levels, animal care staff, and ethics committees to refine procedures and practices in line with the refinement principle of the 3Rs.

Long-Term Prognosis and Treatment of Crop Impaction in Chickens via Ingluviotomy with Local Infiltration Anesthetic: Case Report.

Two hens from two backyard chicken flocks were presented to a private veterinary practice specializing in poultry, both with a chief complaint of crop distention. Crop impaction was diagnosed based on physical examinations and radiographic findings. The hens were admitted for ingluviotomy surgery to remove the impacted fibrous material under local anesthesia. Even though one of the hens was diagnosed with pendulous crop, both hens recovered uneventfully after ingluviotomy and postoperative care. Follow-up examinations and interviews with the owners confirmed excellent long-term recovery.

Reporte de caso- Pronóstico a largo plazo y tratamiento de la impactación del buche en pollos mediante ingluviotomía con anestesia local por infiltración: Reporte de un caso. Dos gallinas de dos parvadas de pollos de traspatio fueron presentadas a una clínica veterinaria privada especializada en aves, ambas aves mostraban como problema principal la distensión del buche. La impactación del buche se diagnosticó con base en exámenes físicos y hallazgos radiográficos. Las gallinas fueron ingresadas para cirugía de inluviotomía para remover el material fibroso impactado bajo anestesia local. A pesar de que a una de las gallinas se le diagnosticó buche pendular, ambas gallinas se recuperaron sin incidentes después de la ingluviotomía y el cuidado postoperatorio. Los exámenes de seguimiento y las entrevistas con los propietarios confirmaron una excelente recuperación a largo plazo.

Validation of Sanitization Practices in Single-use Individually Ventilated Mouse Cages at Standard and Thermoneutral Temperatures.

Vivarium husbandry practices are based on performance data and adhere to applicable regulatory guidelines. Refinements in husbandry and optimization of sanitization protocols improve animal wellbeing and help standardize the microenvironment, contributing to research reproducibility. The objective of this study was to evaluate the microenvironment to establish performance standards for mouse husbandry and sanitization, including housing at standard and thermoneutral temperatures. Male C57BL/6J mice were housed singly and in groups in disposable IVCs on α-cellulose or corncob bedding and microenvironmental indicators (ammonia, carbon dioxide) were evaluated. In addition, microbial bioburden tests (ATP and RODAC) were performed on cages and cage accessories on days 0, 7, 14 and, 28 to 30 after cage change. Water testing and aerobic culture of the waterspout of bottles containing chlorinated water were performed to determine acceptable replacement schedules. Ammonia levels remained below the National Institute of Occupational Safety and Health 8-h recommended exposure limit for humans (25 ppm) at all time points for all housing conditions through day 21 for group-housed mice, and through day 30 for singly housed mice. Microbial bioburden results for cage accessories and water testing were acceptable up to 28 d after cage change (RODAC less than 50 CFU; ATP less than 100,000 RLU) at both standard and thermoneutral housing temperatures. Mice remained clinically healthy throughout the studies. These results support site operating practices and verify extended sanitization recommendations per the Guide of the Care and Use of Laboratory Animals in this disposable IVC environment: group-housed mice receive bottom cage and water bottle change up to every 14 d with full cage change (including lid and accessories) every 28 d, and singly housed mice receive full cage change every 28 to 30 d or sooner.

Solar radiation limits the use of paddocks by laying hens raised in the free-range system.

Laying hens on the free-range systems are susceptible to challenging situations in relation to the rearing environment. Therefore, this work evaluated how solar radiation influences the behavior of laying hens raised in a free-range system, in the Brazilian Savanna. The activities included data collection of meteorological variables and behavioral analysis of 300 commercial laying hens in relation to the frequency of use of indoor and outdoor areas of rearing housing. The solar radiation is the main factor that directly affects the heat gain of production animals, in this experiment had a high amplitude during all day, going from 33.42 to 756.98 W m-2. It was observed that the highest frequency of 79% and 91% use of the barn areas by the hens was at 8 am and 4 pm, respectively. The internal area of the housing was more used by hens 87% and 68% at 12 h and 14 h, respectively. Hens were not observed in the paddocks at noon and 2 pm. Hens spend more than 6 h of the day inside the housing to provide shelter from solar radiation. Which the conclusion the solar radiation influences the behavior of laying hens, at times of the day of the higher incidence of radiation, and high air and global temperatures, it was not observed the presence of hens in the external areas of the housing, especially with the use of the paddocks; at these times the hens seek shelter inside the housing to get away from the incidence of direct solar radiation.

Caracterização dos sistemas de criação de caprinos e ovinos da Ilha de São Luís no estado do Maranhão, Brasil / Characterization of goat and sheep breeding systems at São Luis Island in the state of Maranhão, Brazil

Objetivou-se descrever o perfil dos sistemas de criação de caprinos e ovinos da Ilha de São Luís no estado do Maranhão. Para isso, realizou-se um estudo descritivo, exploratório e quali-quantitativo com 80 produtores rurais selecionados pela técnica Snowball, no período de fevereiro a abril de 2021. Em relação ao perfil do produtor rural, verificou-se predominância do gênero masculino (93,8%), apresentando faixa etária entre 25 a 60 anos (86,2%). A maioria dos produtores apresentavam ensino médio completo (23,8%) ou ensino superior completo (23,8%) e 88,8% dos produtores não estavam vinculados a organizações coletivas, associações, cooperativas ou sindicatos. Nas propriedades rurais o sistema de criação predominante era o semi-extensivo (73,8%) com presença de apriscos (76,2%) e as criações eram consorciadas com caprinos e ovinos (58,8%). A principal causa de morte nos animais verificada foi a verminose (58,8%). Conclui-se que se faz necessário o estabelecimento de medidas de estímulo às criações na Ilha de São Luís por meio de políticas públicas voltadas aos produtores locais, requerendo, sobretudo, assistência técnica, orientações zootécnicas, educação sanitária e acesso ao crédito, para, assim, deixar de ser apenas uma atividade de subsistência e passar a gerar maiores rendas para as famílias produtoras.

The objective of this research was to describe the profile of the goat and sheep rearing systems on the Island of São Luís in the state of Maranhão. For this, a descriptive, exploratory and qualitative-quantitative study was carried out with 80 rural producers selected by the Snowball technique, from February to April 2021. Regarding the profile of the rural producer, there was a predominance of males (93.8%), aging between 25 and 60 years (86.2%). Most producers had completed high school (23.8%) or higher education (23.8%) and 88.8% were not members of collective organizations, associations, cooperatives or unions. In rural properties, the predominant rearing system was semi-extensive (73.8%) with the presence of sheepfolds (76.2%) and mutual goats and sheep farming (58.8%). The main cause of death of animals was warm infections (58.8%). In conclusion, it is necessary to establish measures to support sheep and goat farming on the Island of São Luís through public policies focused on local producers, which require, above all, technical assistance, zootechnical guidelines, health education and access to credit in order to the farms stop being just a subsistence activity and start generating higher incomes for producing families.

Keel bone damage affects behavioral and physiological responses related to stress and fear in two strains of laying hens.

Keel bone damage (KBD) is more prevalent in alternative laying hen housing systems than in conventional cages, and its incidence differs from strain to strain. However, the information of KBD in Lindian chickens, a native Chinese strain, is limited. To investigate the effect of KBD on fearfulness and physiological indicators of stress in Lindian chickens and commercial laying hens, a total of two hundred 25-wk-old chickens (100 Hy-line Brown and 100 Lindian chickens) were studied for 7 wk. The birds were housed in furnished cages with 10 birds per cage for each strain. At 32-wk of age, the birds in each strain were divided into normal (NK), deviated (DK), and fractured (FK) hens according to the keel bone status. Ten birds in each keel bone status per strain were subsequently selected to collect blood for the determination of stress and fear-related indicators, including corticosterone, serotonin, interleukin-1ß, and interleukin-6, and measure fear responses, including novel object test (NOT), human approach test (HAT), and tonic immobility (TI) test. The results showed that egg production was lower and the incidence of keel bone fractures was higher in Lindian chickens than in Hy-line Brown hens (P < 0.05). Lindian chickens showed a significantly increased whole blood serotonin content, NOT-latency, HAT-score, and TI induction times (P < 0.05) and decreased serum interleukin-6 content and TI-duration (P < 0.05) compared with Hy-line Brown hens. Additionally, FK hens had significantly elevated whole blood corticosterone, serum interleukin-1ß and interleukin-6 levels, TI-duration, and NOT-latency (P < 0.05), and a reduced whole blood serotonin content (P < 0.05) compared with NK and DK hens. Our results indicated that KBD affected stress and fear responses, and this impact was mainly reflected by FK hens compared with NK and DK hens. We suggest that keel bone fractures are the main factor impairing hen welfare. Besides, the incidence of keel bone fractures and stress and fear responses of Lindian chickens are more severe than Hy-line Brown laying hens, indicating that the strain type can affect the health and welfare of laying hens.

Keel bone damage (KBD) impairs production performance, welfare, and health in laying hens. This study aimed to compare the incidence of KBD and investigate the effects of KBD on stress and fear in two strains of laying hens. The results showed that commercial Hy-line Brown laying hens had high egg production and low incidence of KBD compared with Lindian chickens, a Chinese native breed. Besides, Lindian chickens had higher blood serotonin content and fear responses to human approach test and novel object test than Hy-line Brown laying hens. In addition, laying hens with keel bone fractures had elevated concentrations of blood corticosterone, interleukin-1ß, and interleukin-6, and had a longer duration of tonic immobility and latency to approach a novel object, as well as reduced blood serotonin content compared with laying hens with normal and deviated keel bone. Overall, keel bone fractures caused stress and fear responses, impairing hen welfare; and behavioral and physiological responses in relation to stress and fear differed between strains of hens.

Bone quality and composition are influenced by egg production, layer line, and oestradiol-17ß in laying hens.

Keel bone fractures are a serious animal welfare problem in laying hens. The aim of the current study was to assess the influence of egg production, oestradiol-17ß, and selection for high laying performance on bone quality. Hens of two layer lines differing in laying performance (WLA: 320 eggs per year, G11: 200 eggs per year) were allocated to four treatment groups. Group S received a deslorelin acetate implant that suppressed egg production. Group E received an implant with the sexual steroid oestradiol-17ß. Group SE received both implants and group C did not receive any implant. In the 63rd week of age, composition and characteristics of the tibiotarsi were assessed using histological analysis, three-point bending test, thermogravimetric analysis, infrared spectroscopy, and two-dimensional X-ray diffraction, respectively. Non-egg-laying hens showed a higher total bone area and a higher relative amount of cortical bone compared to egg-laying hens. Hens of layer line G11 showed a higher relative amount of medullary bone and a higher degree of mineralization of the cortical bone compared to hens of layer line WLA. These differences in bone composition may explain different susceptibility to keel bone fractures in non-egg-laying compared to egg-laying hens as well as in hens of layer lines differing in laying performance. The effect of exogenous oestradiol-17ß on bone parameters varied between the layer lines indicating a genetic influence on bone physiology and the way it can be modulated by hormone substitution.