RESUMO
The quest for chemicals able to operate at selected genomic loci in a spatiotemporally controlled manner is desirable to create manageable DNA damages. Mounting evidence now shows that alternative DNA structures, including G-quadruplexes and branched DNA (or DNA junctions), might hamper proper progression of replication fork, thus triggering DNA damages and genomic instability. Therefore, small molecules that stabilize these DNA structures are currently scrutinized as a promising way to create genomic defects that cannot be dealt with properly by cancer cells. While much emphasis has been recently given to G-quadruplexes and related ligands, we report herein on three-way DNA junctions (TWJ) and related ligands. We first highlight the biological implications of TWJ and their strategic relevance as triggers for replicative stress. Then, we describe a new in vitro high-throughput screening assay, TWJ-Screen, which allows for identifying TWJ ligands with both high affinity and selectivity for TWJ over other DNA structures (duplexes and quadruplexes), in a convenient and unbiased manner as demonstrated by the screening of a library of 25 compounds from different chemical families. TWJ-Screen thus represents a reliable mean to uncover molecular tools able to foster replicative stress through an innovative approach, thus providing new strategic opportunities to combat cancers.
Assuntos
Replicação do DNA/efeitos dos fármacos , DNA Cruciforme/efeitos dos fármacos , Quadruplex G/efeitos dos fármacos , Ensaios de Triagem em Larga Escala , Substâncias Intercalantes/farmacologia , Bibliotecas de Moléculas Pequenas/farmacologia , Sequência de Bases , Dano ao DNA , Corantes Fluorescentes/química , Loci Gênicos , Genoma Humano , Instabilidade Genômica , Humanos , Substâncias Intercalantes/química , Ligantes , Rodaminas/química , Bibliotecas de Moléculas Pequenas/química , Relação Estrutura-AtividadeRESUMO
DNA repair pathways in bacteria that use homologous recombination involve the formation and subsequent resolution of Holliday junction (HJ) intermediates. We have previously identified several hexameric peptides that bind to HJs and interfere with HJ processing enzymes in vitro. The peptide WRWYCR and its D-amino acid stereoisomer wrwycr, are potent antibacterial agents. These hexapeptides must form homodimers in order to interact stably with HJs, and inhibit bacterial growth, and this represents a potential limitation. Herein we describe a disulfide bond-independent inhibitor, WRWYRGGRYWRW and its D-stereoisomer wrwyrggrywrw. We have characterized these single-chain, linear analogs of the hexapeptides, and show that in addition to effectively binding to HJs, and inhibiting the activity of DNA repair enzymes that process HJs, they have equal or greater potency against Gram-positive and Gram-negative bacterial growth. The analogs were also shown to cause DNA damage in bacteria, and disrupt the integrity of the bacterial cytoplasmic membrane. Finally, we found that they have little toxicity toward several eukaryotic cell types at concentrations needed to inhibit bacterial growth.
Assuntos
Bactérias/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , DNA Cruciforme/química , Peptídeos/farmacologia , Antibacterianos/química , Antibacterianos/farmacologia , Reparo do DNA/efeitos dos fármacos , Reparo do DNA/genética , DNA Cruciforme/efeitos dos fármacos , DNA Cruciforme/metabolismo , Recombinação Homóloga/efeitos dos fármacos , Peptídeos/química , Multimerização Proteica , EstereoisomerismoRESUMO
Our lab has isolated hexameric peptides that are structure-selective ligands of Holliday junctions (HJ), central intermediates of several DNA recombination reactions. One of the most potent of these inhibitors, WRWYCR, has shown antibacterial activity in part due to its inhibition of DNA repair proteins. To increase the therapeutic potential of these inhibitors, we searched for small molecule inhibitors with similar activities. We screened 11 small molecule libraries comprising over nine million individual compounds and identified a potent N-methyl aminocyclic thiourea inhibitor that also traps HJs formed during site-specific recombination reactions in vitro. This inhibitor binds specifically to protein-free HJs and can inhibit HJ resolution by RecG helicase, but only showed modest growth inhibition of bacterial with a hyperpermeable outer membrane; nonetheless, this is an important step in developing a functional analog of the peptide inhibitors.
Assuntos
Antibacterianos/química , DNA Cruciforme/efeitos dos fármacos , Nitrocompostos/química , Peptídeos/farmacologia , Propilaminas/química , Recombinação Genética/efeitos dos fármacos , Bibliotecas de Moléculas Pequenas/química , Sequência de Aminoácidos , Antibacterianos/farmacologia , Proteínas de Bactérias/metabolismo , Bacteriófago lambda/enzimologia , Técnicas de Química Combinatória , Reparo do DNA/efeitos dos fármacos , Integrases , Nitrocompostos/farmacologia , Propilaminas/farmacologia , Bibliotecas de Moléculas Pequenas/farmacologiaRESUMO
Chemotherapy has been a major approach to treat cancer. Both constituents of chromatin, chromosomal DNA and the associated chromosomal histone proteins are the molecular targets of the anticancer drugs. Small DNA binding ligands, which inhibit enzymatic processes with DNA substrate, are well known in cancer chemotherapy. These drugs inhibit the polymerase and topoisomerase activity. With the advent in the knowledge of chromatin chemistry and biology, attempts have shifted from studies of the structural basis of the association of these drugs or small ligands (with the potential of drugs) with DNA to their association with chromatin and nucleosome. These drugs often inhibit the expression of specific genes leading to a series of biochemical events. An overview will be given about the latest understanding of the molecular basis of their action. We shall restrict to those drugs, synthetic or natural, whose prime cellular targets are so far known to be chromosomal DNA.