RESUMO
Decidualisation of the endometrium is a key event in early pregnancy, which enables embryo implantation. Importantly, the molecular processes impairing decidualisation in obese mothers are yet to be characterised. We hypothesise that impaired decidualisation in obese mice is mediated by the upregulation of leptin modulators, the suppressor of cytokine signalling 3 (SOCS3) and the protein tyrosine phosphatase non-receptor type 2 (PTPN2), together with the disruption of progesterone (P4)-signal transducer and activator of transcription (STAT3) signalling. After feeding mice with chow diet (CD) or high-fat diet (HFD) for 16 weeks, we confirmed the downregulation of P4 and oestradiol (E2) steroid receptors in decidua from embryonic day (E) 6.5 and decreased proliferation of stromal cells from HFD. In vitro decidualised mouse endometrial stromal cells (MESCs) and E6.5 deciduas from the HFD showed decreased expression of decidualisation markers, followed by the upregulation of SOCS3 and PTPN2 and decreased phosphorylation of STAT3. In vivo and in vitro leptin treatment of mice and MESCs mimicked the results observed in the obese model. The downregulation of Socs3 and Ptpn2 after siRNA transfection of MESCs from HFD mice restored the expression level of decidualisation markers. Finally, DIO mice placentas from E18.5 showed decreased labyrinth development and vascularisation and fetal growth restricted embryos. The present study revealed major defects in decidualisation in obese mice, characterised by altered uterine response to E2 and P4 steroid signalling. Importantly, altered hormonal response was associated with increased expression of leptin signalling modulators SOCS3 and PTPN2. Elevated levels of SOCS3 and PTPN2 were shown to molecularly affect decidualisation in obese mice, potentially disrupting the STAT3-PR regulatory molecular hub.
Assuntos
Decídua , Retardo do Crescimento Fetal , Leptina , Placenta , Transdução de Sinais , Animais , Feminino , Camundongos , Gravidez , Decídua/metabolismo , Decídua/patologia , Dieta Hiperlipídica/efeitos adversos , Retardo do Crescimento Fetal/metabolismo , Retardo do Crescimento Fetal/patologia , Leptina/metabolismo , Camundongos Endogâmicos C57BL , Camundongos Obesos , Obesidade/metabolismo , Obesidade/patologia , Placenta/metabolismo , Progesterona/metabolismo , Proteína Tirosina Fosfatase não Receptora Tipo 2/metabolismo , Proteína Tirosina Fosfatase não Receptora Tipo 2/genética , Fator de Transcrição STAT3/metabolismo , Células Estromais/metabolismo , Proteína 3 Supressora da Sinalização de Citocinas/metabolismo , Proteína 3 Supressora da Sinalização de Citocinas/genéticaRESUMO
Recurrent spontaneous abortion (RSA) is a common pregnancy-related disorder. Cbl proto-oncogene like 1 (CBLL1) is an E3 ubiquitin ligase, which has been reported to vary with the menstrual cycle in the endometrium. However, whether CBLL1 is involved in the occurrence and development of RSA remains unclear. This study aimed to investigate the effects of CBLL1 on RSA. We analyzed the expression of CBLL1 in the decidua of RSA patients, as well as its functional effects on cellular senescence, oxidative stress, and proliferation of human endometrial stromal cells (HESCs). RNA sequencing was employed to identify a key downstream target gene regulated by CBLL1. We found that CBLL1 was upregulated in the decidua of RSA patients. Additionally, overexpression of CBLL1 promoted HESC senescence, increased oxidative stress levels, and inhibited proliferation. Phosphatase and tensin homolog located on chromosome 10 (PTEN) was identified as one of the important downstream target genes of CBLL1. In vivo experiments demonstrated that CBLL1 overexpression in the endometrium caused higher embryo absorption rate in mice. Consequently, elevated CBLL1 expression is a potential cause of RSA, representing a novel therapeutic target for RSA.
Assuntos
Aborto Habitual , Senescência Celular , Endométrio , PTEN Fosfo-Hidrolase , Células Estromais , Adulto , Animais , Feminino , Humanos , Camundongos , Gravidez , Aborto Habitual/metabolismo , Aborto Habitual/genética , Aborto Habitual/patologia , Proliferação de Células , Decídua/metabolismo , Decídua/patologia , Endométrio/metabolismo , Endométrio/patologia , Estresse Oxidativo , Proto-Oncogene Mas , PTEN Fosfo-Hidrolase/metabolismo , PTEN Fosfo-Hidrolase/genética , Células Estromais/metabolismoRESUMO
Polycystic ovary syndrome (PCOS) is a complex common endocrine disorder affecting women of reproductive age. Ovulatory dysfunction is recognized as a primary infertile factor, however, even when ovulation is medically induced and restored, PCOS patients continue to experience reduced cumulative pregnancy rates and a higher spontaneous miscarriage rate. Hyperandrogenism, a hallmark feature of PCOS, affects ovarian folliculogenesis, endometrial receptivity, and the establishment and maintenance of pregnancy. Decidualization denotes the transformation that the stromal compart of the endometrium must undergo to accommodate pregnancy, driven by the rising progesterone levels and local cAMP production. However, studies on the impact of hyperandrogenism on decidualization are limited. In this study, we observed that primary endometrial stromal cells from women with PCOS exhibit abnormal responses to progesterone during in vitro decidualization. A high concentration of testosterone inhibits human endometrial stromal cells (HESCs) decidualization. RNA-Seq analysis demonstrated that pyruvate dehydrogenase kinase 4 (PDK4) expression was significantly lower in the endometrium of PCOS patients with hyperandrogenism compared to those without hyperandrogenism. We also characterized that the expression of PDK4 is elevated in the endometrium stroma at the mid-secretory phase. Artificial decidualization could enhance PDK4 expression, while downregulation of PDK4 leads to abnormal decidualization both in vivo and in vitro. Mechanistically, testosterone excess inhibits IGFBP1 and PRL expression, followed by phosphorylating of AMPK that stimulates PDK4 expression. Based on co-immunoprecipitation analysis, we observed an interaction between SIRT1 and PDK4, promoting glycolysis to facilitate decidualization. Restrain of AR activation resumes the AMPK/SIRT1/PDK4 pathway suppressed by testosterone excess, indicating that testosterone primarily acts on decidualization through AR stimulation. Androgen excess in the endometrium inhibits decidualization by disrupting the AMPK/SIRT1/PDK4 signaling pathway. These data demonstrate the critical roles of endometrial PDK4 in regulating decidualization and provide valuable information for understanding the underlying mechanism during decidualization.
Assuntos
Proteínas Quinases Ativadas por AMP , Endométrio , Síndrome do Ovário Policístico , Sirtuína 1 , Células Estromais , Humanos , Feminino , Síndrome do Ovário Policístico/metabolismo , Síndrome do Ovário Policístico/patologia , Células Estromais/metabolismo , Células Estromais/patologia , Células Estromais/efeitos dos fármacos , Sirtuína 1/metabolismo , Sirtuína 1/genética , Endométrio/metabolismo , Endométrio/patologia , Endométrio/efeitos dos fármacos , Proteínas Quinases Ativadas por AMP/metabolismo , Adulto , Hiperandrogenismo/metabolismo , Hiperandrogenismo/patologia , Decídua/metabolismo , Decídua/patologia , Testosterona/metabolismo , Testosterona/farmacologia , Androgênios/farmacologia , Androgênios/metabolismo , Progesterona/metabolismo , Progesterona/farmacologia , Piruvato Desidrogenase Quinase de Transferência de Acetil/metabolismo , Piruvato Desidrogenase Quinase de Transferência de Acetil/genética , Transdução de Sinais/efeitos dos fármacosRESUMO
Endometriosis (EMs)-related infertility commonly has decreased endometrial receptivity and normal decidualization is the basis for establishing and maintaining endometrial receptivity. However, the potential molecular regulatory mechanisms of impaired endometrial decidualization in patients with EMs have not been fully clarified. We confirmed the existence of reduced endometrial receptivity in patients with EMs by scanning electron microscopy and quantitative real-time PCR. Here we identified an lncRNA, named BMPR1B-AS1, which is significantly downregulated in eutopic endometrium in EMs patients and plays an essential role in decidual formation. Furthermore, RNA pull-down, mass spectrometry, RNA immunoprecipitation, and rescue analyses revealed that BMPR1B-AS1 positively regulates decidual formation through interaction with the RNA-binding protein insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2). Downregulation of IGF2BP2 led to a decreased stability of BMPR1B-AS1 and inhibition of activation of the SMAD1/5/9 pathway, an inhibitory effect which diminished decidualization in human endometrial stromal cells (hESCs) decidualization. In conclusion, our identified a novel regulatory mechanism in which the IGF2BP2-BMPR1B-AS1-SMAD1/5/9 axis plays a key role in the regulation of decidualization, providing insights into the potential link between abnormal decidualization and infertility in patients with EMs, which will be of clinical significance for the management and treatment of infertility in patients with EMs.
Assuntos
Endometriose , RNA Longo não Codificante , Proteínas de Ligação a RNA , Adulto , Feminino , Humanos , Receptores de Proteínas Morfogenéticas Ósseas Tipo I/metabolismo , Receptores de Proteínas Morfogenéticas Ósseas Tipo I/genética , Decídua/metabolismo , Decídua/patologia , Endometriose/metabolismo , Endometriose/genética , Endometriose/patologia , Endométrio/metabolismo , Endométrio/patologia , Infertilidade Feminina/metabolismo , Infertilidade Feminina/genética , Infertilidade Feminina/patologia , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Proteínas de Ligação a RNA/metabolismo , Proteínas de Ligação a RNA/genética , Transdução de Sinais , Células Estromais/metabolismo , Proteínas Smad , Adulto JovemRESUMO
Ovarian endometriosis is a common gynecological disease, and one of its most significant symptoms is infertility. In patients with endometriosis, defects in endometrial decidualization lead to impaired endometrial receptivity and embryo implantation, thus affecting early pregnancy and women's desire to have children. However, the mechanisms underlying the development of endometriosis and its associated defective decidualization are unclear. We find that NEK2 expression is increased in the ectopic and eutopic endometrium of patients with endometriosis. Meanwhile, NEK2 interacts with FOXO1 and phosphorylates FOXO1 at Ser184, inhibiting the stability of the FOXO1 protein. Importantly, NEK2-mediated phosphorylation of FOXO1 at Ser184 promotes cell proliferation, migration, invasion and impairs decidualization. Furthermore, INH1, an inhibitor of NEK2, inhibits the growth of ectopic lesions in mouse models of endometriosis and promotes endometrial decidualization in mouse models of artificially induced decidualization. Taken together, these findings indicate that NEK2 regulates the development of endometriosis and associated disorders of decidualization through the phosphorylation of FOXO1, providing a new therapeutic target for its treatment.
Assuntos
Proliferação de Células , Endometriose , Endométrio , Proteína Forkhead Box O1 , Quinases Relacionadas a NIMA , Feminino , Endometriose/metabolismo , Endometriose/patologia , Proteína Forkhead Box O1/metabolismo , Proteína Forkhead Box O1/genética , Humanos , Animais , Fosforilação , Camundongos , Quinases Relacionadas a NIMA/metabolismo , Quinases Relacionadas a NIMA/genética , Endométrio/metabolismo , Endométrio/patologia , Movimento Celular , Decídua/metabolismo , Decídua/patologia , Adulto , Modelos Animais de DoençasRESUMO
The pathogenesis of preeclampsia (PE) has not been elucidated, but immune imbalance is known to be one of the main pathogeneses. Dysfunction of decidual macrophages can lead to PE, and the PD-1/PD-L1 signaling pathway is associated with macrophage polarization. However, the relationship between the influence of the PD-1/PD-L1 signaling pathway on macrophage polarization and the onset of PE has not been fully elucidated. In this study, we analyzed the expression of CD68, iNOS, CD206, PD-1 and PD-L1 and the coexpression of CD68+PD-1+ and CD68+PD-L1+ in the decidual tissue of PE patients (n= 18) and healthy pregnant women (n=20). We found that CD68 and iNOS expression was increased in the decidua of PE patients (P < 0.001) and that CD206, PD-1 and PD-L1 expression and CD68+PD-1+ and CD68+PD-L1+ coexpression were decreased (P < 0.001). To assess the influence of the PD-1/PD-L1 signaling pathway on macrophage polarization, we added an anti-PD-1 mAb (pembrolizumab) or an anti-PD-L1 mAb (durvalumab) during THP-1 differentiation into M1 macrophages. Then, we detected the polarization of CD68+CD80+ macrophages and the expression of iNOS. To examine the effect of macrophage polarization on the invasion ability of trophoblast cells, macrophages were cocultured with HTR8/SVneo cells, and the invasion ability of HTR8/SVneo cells was detected via transwell assays. We found that CD68+CD80+ macrophage polarization was enhanced (P<0.05) and that iNOS expression was greater (P<0.01) in the pembrolizumab group. In the durvalumab group, CD68+CD80+ macrophage polarization and iNOS expression were also increased (P<0.05 and P<0.001). Compared with that in the untreated group, the aggressiveness of HTR8/SVneo cells was decreased in both the pembrolizumab group (P < 0.01) and the durvalumab group (P < 0.001). These findings indicate that the PD-1/PD-L1 signaling pathway may play an important role in the pathogenesis of PE by influencing macrophage polarization and reducing the invasion ability of trophoblasts.
Assuntos
Antígeno B7-H1 , Decídua , Macrófagos , Pré-Eclâmpsia , Receptor de Morte Celular Programada 1 , Transdução de Sinais , Humanos , Feminino , Pré-Eclâmpsia/imunologia , Pré-Eclâmpsia/patologia , Pré-Eclâmpsia/metabolismo , Gravidez , Antígeno B7-H1/metabolismo , Antígeno B7-H1/imunologia , Receptor de Morte Celular Programada 1/metabolismo , Decídua/imunologia , Decídua/patologia , Decídua/metabolismo , Macrófagos/imunologia , Macrófagos/metabolismo , Transdução de Sinais/imunologia , Adulto , Antígenos CD/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Ativação de Macrófagos/imunologia , Células THP-1RESUMO
In brief: Failure to induce mesenchymal-epithelial transition (MET) during stromal cell decidualization can lead to consequences such as impaired fertility in patients with endometriosis. METTL3-mediated m6A modification plays an important role in attenuating MET and defective decidualization of endometrial stromal cells and contributes to the development of reduced endometrial receptivity in endometriosis. Abstract: Mesenchymal-epithelial transition (MET)-mediated endometrial decidualization is pivotal for achieving endometrial receptivity and successful pregnancy. We observed blockade of MET in the eutopic secretory endometrium of patients with endometriosis, but the underlying mechanism is unknown. In this study, real-time PCR was used to detect PRL and IGFBP1 expression, whereas western blotting was used to detect the expression of MET markers and METTL3. Phalloidin staining was used to identify changes in cell morphology. M6A levels were quantified using a colorimetric method and m6A dot blots, and functional analysis was performed using spheroid adhesion assays. We first found that increased E-cadherin expression was accompanied by decreased vimentin and Slug expression in the eutopic secretory endometrium of individuals with endometriosis. We also detected a significant increase in both the m6A level and the expression of the related methyltransferase METTL3. Finally, METTL3 expression was negatively correlated with PRL, IGFBP1, and MET markers expression. Collectively, our findings suggest that METTL3 mediates m6A modification, thereby inhibiting MET formation within the eutopic secretory endometrium of patients with endometriosis. Increased METTL3-mediated m6A modification plays a crucial role in attenuating MET formation and decidualization impairment in endometrial stromal cells, ultimately contributing to compromised endometrial receptivity in individuals with endometriosis. These insights could lead to the identification of potential therapeutic targets for improving both endometrial receptivity and pregnancy rate among individuals affected by endometriosis.
Assuntos
Endometriose , Endométrio , Transição Epitelial-Mesenquimal , Metiltransferases , Células Estromais , Feminino , Humanos , Endometriose/metabolismo , Endometriose/patologia , Endometriose/genética , Endométrio/metabolismo , Endométrio/patologia , Adulto , Metiltransferases/metabolismo , Metiltransferases/genética , Células Estromais/metabolismo , Células Estromais/patologia , Proteína 1 de Ligação a Fator de Crescimento Semelhante à Insulina/metabolismo , Proteína 1 de Ligação a Fator de Crescimento Semelhante à Insulina/genética , Decídua/metabolismo , Decídua/patologia , Adenosina/metabolismo , Adenosina/análogos & derivados , Caderinas/metabolismo , Caderinas/genéticaRESUMO
Spontaneous abortion is the most common complication in early pregnancy, the exact etiology of most cases cannot be determined. Emerging studies suggest that mutations in ciliary genes may be associated with progression of pregnancy loss. However, the involvement of primary cilia on spontaneous abortion and the underlying molecular mechanisms remains poorly understood. We observed the number and length of primary cilia were significantly decreased in decidua of spontaneous abortion in human and lipopolysaccharide (LPS)-induced abortion mice model, accompanied with increased expression of proinflammatory cytokines interleukin (IL)-1ß, IL-6, and tumor necrosis factor (TNF)-α. The length of primary cilia in human endometrial stromal cell (hESC) was significantly shortened after TNF-α treatment. Knocking down intraflagellar transport 88 (IFT88), involved in cilia formation and maintenance, promoted the expression of TNF-α. There was a reverse regulatory relationship between cilia shortening and TNF-α expression. Further research found that shortened cilia impair decidualization in hESC through transforming growth factor (TGF)-ß/SMAD2/3 signaling. Primary cilia were impaired in decidua tissue of spontaneous abortion, which might be mainly caused by inflammatory injury. Primary cilia abnormalities resulted in dysregulation of TGF-ß/SMAD2/3 signaling transduction and decidualization impairment, which led to spontaneous abortion.
Assuntos
Aborto Espontâneo , Cílios , Transdução de Sinais , Proteína Smad2 , Proteína Smad3 , Fator de Crescimento Transformador beta , Feminino , Cílios/metabolismo , Cílios/patologia , Aborto Espontâneo/metabolismo , Aborto Espontâneo/patologia , Humanos , Proteína Smad2/metabolismo , Proteína Smad2/genética , Fator de Crescimento Transformador beta/metabolismo , Fator de Crescimento Transformador beta/genética , Animais , Proteína Smad3/metabolismo , Proteína Smad3/genética , Gravidez , Camundongos , Decídua/metabolismo , Decídua/patologia , Fator de Necrose Tumoral alfa/metabolismo , Células Estromais/metabolismo , Células Estromais/patologiaRESUMO
BACKGROUND: Preeclampsia is a serious disease of pregnancy that lacks early diagnosis methods or effective treatment, except delivery. Dysregulated uterine immune cells and spiral arteries are implicated in preeclampsia, but the mechanistic link remains unclear. METHODS: Single-cell RNA sequencing and spatial transcriptomics were used to identify immune cell subsets associated with preeclampsia. Cell-based studies and animal models including conditional knockout mice and a new preeclampsia mouse model induced by recombinant mouse galectin-9 were applied to validate the pathogenic role of a CD11chigh subpopulation of decidual macrophages (dMφ) and to determine its underlying regulatory mechanisms in preeclampsia. A retrospective preeclampsia cohort study was performed to determine the value of circulating galectin-9 in predicting preeclampsia. RESULTS: We discovered a distinct CD11chigh dMφ subset that inhibits spiral artery remodeling in preeclampsia. The proinflammatory CD11chigh dMφ exhibits perivascular enrichment in the decidua from patients with preeclampsia. We also showed that trophoblast-derived galectin-9 activates CD11chigh dMφ by means of CD44 binding to suppress spiral artery remodeling. In 3 independent preeclampsia mouse models, placental and plasma galectin-9 levels were elevated. Galectin-9 administration in mice induces preeclampsia-like phenotypes with increased CD11chigh dMφ and defective spiral arteries, whereas galectin-9 blockade or macrophage-specific CD44 deletion prevents such phenotypes. In pregnant women, increased circulating galectin-9 levels in the first trimester and at 16 to 20 gestational weeks can predict subsequent preeclampsia onset. CONCLUSIONS: These findings highlight a key role of a distinct perivascular inflammatory CD11chigh dMφ subpopulation in the pathogenesis of preeclampsia. CD11chigh dMφ activated by increased galectin-9 from trophoblasts suppresses uterine spiral artery remodeling, contributing to preeclampsia. Increased circulating galectin-9 may be a biomarker for preeclampsia prediction and intervention.
Assuntos
Decídua , Galectinas , Macrófagos , Pré-Eclâmpsia , Remodelação Vascular , Pré-Eclâmpsia/metabolismo , Pré-Eclâmpsia/imunologia , Gravidez , Feminino , Animais , Galectinas/metabolismo , Macrófagos/metabolismo , Macrófagos/imunologia , Macrófagos/patologia , Camundongos , Humanos , Decídua/metabolismo , Decídua/patologia , Camundongos Knockout , Útero/metabolismo , Útero/irrigação sanguínea , Modelos Animais de Doenças , Receptores de Hialuronatos/metabolismo , Receptores de Hialuronatos/genética , Estudos Retrospectivos , Camundongos Endogâmicos C57BL , Antígenos CD11RESUMO
BACKGROUND: Clinically, recurrent spontaneous abortion (RSA) is a pregnancy illness that is difficult to treat. Impaired decidualization is a documented cause of RSA, but the etiology and mechanism are still unknown. cAMP-responsive element binding protein 5 (CREB5) is a member of the ATF/CREB family. CREB5 has been reported to be related to pathological pregnancy, but there are few related studies on this topic in patients with RSA, and the underlying mechanism is unclear. METHODS: We collected decidual tissues from RSA patients and healthy pregnant women to measure the expression level of CREB5, PRL, IGFBP1, ATG5, LC3B, and SQSTM/p62. Then, the changes in CREB5 expression and autophagy levels were measured in human endometrial stromal cells (hESCs) during decidualization. The expression levels of PRL and IGFBP1 were tested in sh-CREB5/ov-CREB5 hESCs after decidualization induction, and the autophagy level in sh-CREB5/ov-CREB5 hESCs was measured without decidualization induction. The decidualization ability of sh-CREB5 and ov-CREB5 hESCs treated with an autophagy inducer or inhibitor was measured. To investigate the effect of CREB5 in hESCs on the invasion and migration of HTR8/SVneo cells, we performed a coculture experiment. Finally, we examined the expression of CREB5 and autophagy key proteins in mouse decidual tissues by constructing an abortion mouse model. RESULTS: In our study, we found that the expression of CREB5 was unusually elevated in the uterine decidua of RSA patients, but the expression of PRL, IGFBP1, and autophagy were decreased. During the decidualization of hESCs, the expression of CREB5 gradually decreases in a time-dependent manner with increasing autophagy. Moreover, by knocking down or overexpressing CREB5 in hESCs, it was found that CREB5 can impair decidualization and reduce autophagy in hESCs. Furthermore, the damage caused by CREB5 in terms of decidualization can be reversed by the addition of an autophagy inducer (rapamycin). In addition, CREB5 can increase the secretion of proteins (IL-1ß and TGF-ß1) in hESCs to inhibit trophoblast invasion and migration. CONCLUSIONS: Our data support the supposition that CREB5 disturbs the decidualization of endometrial stromal cells and interactions at the maternal-fetal interface by inhibiting autophagy and that its abnormal upregulation and dysfunction may lead to RSA. It may function as a diagnostic and therapeutic target for RSA. Similarly, we found that in the spontaneous abortion mouse model, the expression of CREB5 in the decidual tissue of the abortion group was significantly increased, and autophagy was decreased.
Assuntos
Aborto Habitual , Autofagia , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico , Decídua , Feminino , Autofagia/fisiologia , Humanos , Gravidez , Decídua/metabolismo , Decídua/patologia , Aborto Habitual/metabolismo , Aborto Habitual/patologia , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Animais , Adulto , Camundongos , Células Estromais/metabolismo , Relações Materno-Fetais/fisiologia , Troca Materno-Fetal/fisiologia , Endométrio/metabolismo , Endométrio/patologia , Proteína A de Ligação a Elemento de Resposta do AMP CíclicoRESUMO
A 43-year-old female presented with blood loss and persistent abdominal pain at 14 weeks of gestation. Ultrasound examination and subsequent magnetic resonance imaging (MRI) revealed bilateral multicystic uterine adnexa. Exploratory laparotomy was performed at 17 weeks of gestation and bilateral serous ovarian adenocarcinoma FIGO stage IIIC was diagnosed. Complete cytoreductive surgery (CRS) was not feasible at that moment. Nine days after the exploratory laparotomy, immature rupture of membranes and contractions occurred and she delivered a premature boy after 19 weeks of gestation. Pathological examination of the placenta revealed that her ovarian cancer metastasized to the membranes. We describe the first case of ovarian cancer metastasized to the decidua of the placental membranes with histological, immunohistochemical, and molecular confirmation. This case highlights the importance of conscientious evaluation of placenta and membranes in pregnant women with ovarian cancer.
Assuntos
Neoplasias Ovarianas , Complicações Neoplásicas na Gravidez , Humanos , Feminino , Neoplasias Ovarianas/patologia , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/secundário , Gravidez , Adulto , Complicações Neoplásicas na Gravidez/patologia , Complicações Neoplásicas na Gravidez/diagnóstico , Decídua/patologia , Cistadenocarcinoma Seroso/secundário , Cistadenocarcinoma Seroso/diagnóstico , Cistadenocarcinoma Seroso/patologia , Cistadenocarcinoma Seroso/metabolismo , Placenta/patologia , Biomarcadores Tumorais/metabolismo , Biomarcadores Tumorais/análiseRESUMO
BACKGROUND: Placenta accreta spectrum disorders are associated with severe maternal morbidity and mortality. Placenta accreta spectrum disorders involve excessive adherence of the placenta preventing separation at birth. Traditionally, this condition has been attributed to excessive trophoblast invasion; however, an alternative view is a fundamental defect in decidual biology. OBJECTIVE: This study aimed to gain insights into the understanding of placenta accreta spectrum disorder by using single-cell and spatially resolved transcriptomics to characterize cellular heterogeneity at the maternal-fetal interface in placenta accreta spectrum disorders. STUDY DESIGN: To assess cellular heterogeneity and the function of cell types, single-cell RNA sequencing and spatially resolved transcriptomics were used. A total of 12 placentas were included, 6 placentas with placenta accreta spectrum disorder and 6 controls. For each placenta with placenta accreta spectrum disorder, multiple biopsies were taken at the following sites: placenta accreta spectrum adherent and nonadherent sites in the same placenta. Of note, 2 platforms were used to generate libraries: the 10× Chromium and NanoString GeoMX Digital Spatial Profiler for single-cell and spatially resolved transcriptomes, respectively. Differential gene expression analysis was performed using a suite of bioinformatic tools (Seurat and GeoMxTools R packages). Correction for multiple testing was performed using Clipper. In situ hybridization was performed with RNAscope, and immunohistochemistry was used to assess protein expression. RESULTS: In creating a placenta accreta cell atlas, there were dramatic difference in the transcriptional profile by site of biopsy between placenta accreta spectrum and controls. Most of the differences were noted at the site of adherence; however, differences existed within the placenta between the adherent and nonadherent site of the same placenta in placenta accreta. Among all cell types, the endothelial-stromal populations exhibited the greatest difference in gene expression, driven by changes in collagen genes, namely collagen type III alpha 1 chain (COL3A1), growth factors, epidermal growth factor-like protein 6 (EGFL6), and hepatocyte growth factor (HGF), and angiogenesis-related genes, namely delta-like noncanonical Notch ligand 1 (DLK1) and platelet endothelial cell adhesion molecule-1 (PECAM1). Intraplacental tropism (adherent versus non-adherent sites in the same placenta) was driven by differences in endothelial-stromal cells with notable differences in bone morphogenic protein 5 (BMP5) and osteopontin (SPP1) in the adherent vs nonadherent site of placenta accreta spectrum. CONCLUSION: Placenta accreta spectrum disorders were characterized at single-cell resolution to gain insight into the pathophysiology of the disease. An atlas of the placenta at single cell resolution in accreta allows for understanding in the biology of the intimate maternal and fetal interaction. The contributions of stromal and endothelial cells were demonstrated through alterations in the extracellular matrix, growth factors, and angiogenesis. Transcriptional and protein changes in the stroma of placenta accreta spectrum shift the etiologic explanation away from "invasive trophoblast" to "loss of boundary limits" in the decidua. Gene targets identified in this study may be used to refine diagnostic assays in early pregnancy, track disease progression over time, and inform therapeutic discoveries.
Assuntos
Descolamento Prematuro da Placenta , Placenta Acreta , Doenças Placentárias , Gravidez , Feminino , Recém-Nascido , Humanos , Placenta Acreta/terapia , Células Endoteliais , Placenta/patologia , Doenças Placentárias/patologia , Peptídeos e Proteínas de Sinalização Intercelular , Decídua/patologia , Endotélio/patologiaRESUMO
La reacción decidual o decidousis es un fenómeno fisiológicos del embarazo que se produce por metaplasia de células pluripontenciales del mesénquima subcelómico inducida por la progesterona. Habitualmente es asintomática y se la encuentran incidentalmente en tejidos abdominales extirpados quirurgicamente pero en raras ocasiones puede manifestarse con síntomas que varían de acuerdo con su localización. La localización apendicular con clínica de abdomen agudo es excepcional. Comunicamos un caso de deciduosis apendicular que se presentó con abdomen agudo durante el tercer trimestre del embarazo.
Assuntos
Humanos , Feminino , Gravidez , Adulto , Dor Abdominal/etiologia , Apêndice , Doenças do Ceco/complicações , Decídua , Complicações na Gravidez , Apendicite/diagnóstico , Apêndice/patologia , Decídua/patologiaRESUMO
En 1994 en un estudio realizado por nuestro grupo en colabaración con la Dra. Fuksman sobre 1.200 placentas correspondientes a embarazos de alto riesgo, se halló la presencia de villitis en el 5.6 por ciento de las mismas. La histopatología detectada en ese momento fue deciduitis linfocitaria y aumento de fibrina perirvellositaria asociada con hipoirrigación e infarto placentario. Hallamos que en el 55 por ciento de las placentas con villitis los recién nacidos presentaban RCIU con respecto al 10 por ciento de los controles, con un PA de 32 por ciento en las villitis y el 83 por ciento en los controles (3). En ese material se estudiaron 68 placentas con villitis y 68 placentas sin villitis como grupo control. En 1996 demostramos en ese mismo material mediante la técnica de anticuerpos monoclonales, sobre cortes de placenta estudiando las subpoblaciones linfocitarias de las villitis, que el 50 por ciento eran CD4 (linfocitos helper), 18 por ciento CD8 (linfocitos supresoreslcitotóxicos) y 10 por ciento Leu19 (Natural Killer) pero lo significativo y anormal es que hallamos que el 65 por ciento de los linfocitos expresaban antígenos de histocompatibilidad clase II DR (40). En 1998 Jacques y Col publicaron datos similares. En 1999 comunicamos que en el informe histopatológico de material de legrado de pacientes abortadoras de causa inmunológica la descripción de villitis en un 20 por ciento de los casos. Estudios realizados en colabaración con la Dra. Zenclussen con ese material nos permitió publicar recientemente la presencia de altos niveles de Interleuquina 6(IL-6) y receptor de IL-6 en suero. El objetivo de este estudio es investigar en placentas de pacientes abortadoras recurrentes la expresión de IL-6 y sus receptores gp80 y gp130 en trece muestras de material de raspado de abortos del primer trimestre mediante la técnica de inmunofluorescencia. Como control se utilizaron cortes de placentas de embarazos normales a término. Nuestros hallazgos muestran la presencia de depósitos de IL-6 y de receptores de IL-6 con un patrón granular para las tres moléculas especificamente en el sinciciotrofoblasto mientras que fue negativo para tres en el citotrofoblasto. En los cortes de placentas normales no se hallaron en ningún caso dichos depósitos. Concluímos de todos los hallazgos antes sintetizados...
Assuntos
Humanos , Recém-Nascido , Feminino , Gravidez , Aborto Espontâneo/etiologia , Interleucina-6/efeitos adversos , Placenta/patologia , Trofoblastos/patologia , Aborto Espontâneo/imunologia , Anticorpos Monoclonais , Antígenos de Histocompatibilidade Classe II , Linfócitos T CD4-Positivos , Linfócitos T CD8-Positivos , Corioamnionite/imunologia , Corioamnionite/fisiopatologia , Decídua/patologia , Antígenos de Histocompatibilidade , Imuno-Histoquímica , Primeiro Trimestre da Gravidez/efeitos dos fármacos , Gravidez de Alto Risco/imunologia , Receptores de Interleucina-6/imunologiaRESUMO
Se presentan 20 casos consecutivos de acretismo placentario en embarazadas con cesáreas previas ocurridos en el Servicio de Obstetricia del Hospital Materno Infantil de Mar del Plata desde el 1/1/92 al 31/12/93, todos con confirmación anatomopatológica en las piezas de histerectomía. En dicho período hubo 11410 nacimientos y se realizaron 27 por ciento de cesáreas abdominales, de las cuales el 14 por ciento fueron primer cesárea y el 13 por ciento restante iterativas. En los 11410 nacimientos se diagnosticaron 23 acretismos, de los cuales 20 correspondían a pacientes con cesárea anterior y 3 (que no fueron incluídos en este trabajo), no presentaban sección de cesárea previa. Nuestra incidencia de acretismo fue de 1/496 nacimientos. El porcentaje de riesgo de hacer acretismo que tuvieron las embarazadas con placenta previa segmentaria y cicatriz de cesárea anterior fue incrementándose significativamente de acuerdo al número de cesáreas previas, así con 1 ó 2 cicatrices de cesáreas previas el porcentaje de riesgo fue del 5 por ciento, con 3 cesáreas previas fue del 22 por ciento, y con 4 o más el riesgo se elevó al 70 por ciento. El estudio histopatológico de las piezas de histerectomía reveló que el factor predominante fue la ausencia de la decidua. La etiología de esta deficiencia decidual muy posiblemente haya sido la sección de cesárea previa. El objetivo de este trabajo es demostrar dicho aumento en el porcentaje de riesgo de hacer acretismo en estas embarazadas, clasificar a las mismas dentro del grupo de pacientes de "alto riesgo obstétrico", intensificar y normatizar métodos de diagnóstico anteparto, sugerir conductas prequirúrgicas especiales y una vía de abordaje uterino diferente a la habitual
Assuntos
Humanos , Feminino , Gravidez , Recesariana/efeitos adversos , Cesárea/efeitos adversos , Placenta Acreta , Decídua/patologia , Placenta Acreta/diagnóstico , Placenta Acreta/epidemiologia , Placenta Acreta/etiologia , Placenta Acreta/patologia , Placenta Acreta/cirurgiaRESUMO
Se analiza el valor de la identificación del quiste decidual con ecografía transvaginal como signo precoz en el embarazo ectópico
Assuntos
Humanos , Feminino , Gravidez , Adulto , Cistos , Decídua , Gravidez Ectópica , Cistos/etiologia , Decídua/patologia , Gravidez Ectópica/diagnósticoRESUMO
El estudio histológico del tejido decidual en la rata pseudopreñada, reveló una onda constante de figuras mitóticas que, partiendo de la decidua antimesometrial, se desplazó hacia la decidua mesometrial y, finalmente, hacia el triángulo mesometrial. Este y otros cambios morfológicos, como el estudio cualitativo de la superficie endometrial con microscopia eletrónica, fueron comparados con aquellos encontrados en la rata pseudopreñada diabética. No se encontraron diferencias estadísticamente significativas en cuanto a peso uterino, distribución de figuras mitóticas, ni distribución de glicógeno en los días 7 y 10 de pseudopreñez. Tampoco se observaron diferencias cualitativas entre la superficie epitelial del grupo control y diabético. El día 13, sin embargo, se encontraron tres diferencias estadísticamente significativas. Se observó en el grupo diabético: 1) caída en el peso uterino; 2) menor incidencia de figuras mitóticas en el triángulo mesometrial, y 3) menor grado de diferenciación de las arterias espirales